[A method of preparing sections for express histological diagnosis]. / Sposob izgotovleniia preparatov dlia srochnoi gistologicheskoi diagnostiki.

The method of improving quality of histological slides for intrasurgical diagnosis is proposed. The method consists in preliminary microwave processing of the slice of surgical material before putting in into cryostat for freezing and sections preparation. A slice of surgical material was first immersed in 10 ml of physiological solution (pH = 7.2). Then it was exposed to microwave radiation (2.45 GHz, 1 W/cm2) for 1.5 min. After that the slice was put into cryostat and frozen. Microwave processing is done in a home microwave oven or working chamber of microwave histoprocessor. Essential advantage in quality of slides is obtained.

[Histologic tissue processing in an automated microwave histoprocessor]. / Gistologicheskaia provodka tkanei v avtomaticheskom mikrovolnovom gistoprotsessore.

A new vacuum histoprocessor has been tested as well as new more effective protocols to work with this processor. The device allows histoprocessing of 50 samples simultaneously of surgery, biopsy or autopsy material during 1-1.5 h. Apart from the standard protocols, new protocols with a much lower (5-10 times) expenditure of the chemicals can be implemented due to combination of microwaves and vacuum. Each protocol was tested repeatedly (20 and more times) with stable good quality of microscopic image.

[Acceleration of histologic tissue processing and decalcification using a microwave oven]. / Uskorenie gistologicheskoi provodki tkanei i dekal'tsinatsii s pomoshch'iu mikrovolnovoi pechi.

Feasibility of significant acceleration of histoprocessing by using domestic microwave oven is shown. Two histoprocessing protocols for a single bit and many bits of surgery, biopsy and autopsy materials have been introduced. The design of a special container is described. The histoprocessing period was reduced to 2 hrs. Significant acceleration (10 to 20 times) of decalcification of bone tissue by using domestic microwave oven was achieved. Decalcification took from 3 to 8 hrs in good quality of the microscopic images.