Green solutions for antibiotic pollution: Assessing the phytoremediation potential of aquatic macrophytes in wastewater treatment plants.

We compared the ability of one emergent (Sagittaria montevidensis), two floating (Salvinia minima and Lemna gibba), and one heterophyllous species (Myriophyllum aquaticum) to simultaneously remove sulfamethoxazole, sulfadiazine, ciprofloxacin, enrofloxacin, norfloxacin, levofloxacin, oxytetracycline, tetracycline, doxycycline, azithromycin, amoxicillin, and meropenem from wastewater in a mesocosm-scale constructed wetland over 28 days. Antibiotic concentrations in plants and effluent were analyzed using an LC-MS/MS to assess the removal rates and phytoremediation capacities. M. aquaticum did not effectively mitigate contamination due to poor tolerance and survival in effluent conditions. S. minima and L. gibba demonstrated superior efficiency, reducing the antibiotic concentrations to undetectable levels within 14 days, while S. montevidensis achieved this result by day 28. Floating macrophytes emerge as the preferable choice for remediation of antibiotics compared to emergent and heterophyllous species. Antibiotics were detected in plant tissues at concentrations ranging from 0.32 to 29.32 ng g-1 fresh weight, highlighting macrophytes' ability to uptake and accumulate these contaminants. Conversely, non-planted systems exhibited a maximum removal rate of 65%, underscoring the persistence of these molecules in natural environments, even after the entire experimental period. Additionally, macrophytes improved effluent quality regardless of species by reducing total soluble solids and phosphate concentrations and mitigating ecotoxicological effects. This study underscores the potential of using macrophytes in wastewater treatment plants to enhance overall efficiency and prevent environmental contamination by antibiotics, thereby mitigating the harmful impact on biota and antibiotic resistance. Selecting appropriate plant species is crucial for successful phytoremediation in constructed wetlands, and actual implementation is essential to validate their effectiveness and practical applicability.

Emerging threat: Antimicrobial resistance proliferation during epidemics - A case study of the SARS-CoV-2 pandemic in South Brazil.

The escalating global concern of antimicrobial resistance poses a significant challenge to public health. This study delved into the occurrence of resistant bacteria and antimicrobial resistance genes in the waters and sediments of urban rivers and correlated this emergence and the heightened use of antimicrobials during the COVID-19 pandemic. Isolating 45 antimicrobial-resistant bacteria across 11 different species, the study identifies prevalent resistance patterns, with ceftriaxone resistance observed in 18 isolates and ciprofloxacin resistance observed in 13 isolates. The detection of extended-spectrum ß-lactamases, carbapenemases, and acquired quinolone resistance genes in all samples underscores the gravity of the situation. Comparison with a pre-pandemic study conducted in the same rivers in 2019 reveals the emergence of previously undetected new resistant species, and the noteworthy presence of new resistant species and alterations in resistance profiles among existing species. Notably, antimicrobial concentrations in rivers increased during the pandemic, contributing significantly to the scenario of antimicrobial resistance observed in these rivers. We underscore the substantial impact of heightened antimicrobial usage during epidemics, such as COVID-19, on resistance in urban rivers. It provides valuable insights into the complex dynamics of antimicrobial resistance in environmental settings and calls for comprehensive approaches to combat this pressing global health issue, safeguarding both public and environmental health.

COVID-19 pharmaceuticals in aquatic matrices: The threatening effects over cyanobacteria and microalgae.

Water contamination by pharmaceuticals is a global concern due to their potential negative effects on aquatic ecosystems and human health. This study examined the presence of three repositioned drugs used for COVID-19 treatment: azithromycin (AZI), ivermectin (IVE) and hydroxychloroquine (HCQ) in water samples collected from three urban rivers in Curitiba, Brazil, during August and September 2020. We conducted a risk assessment and evaluated the individual (0, 2, 4, 20, 100 and 200 µg.L-1) and combined (mix of the drugs at 2 µg.L-1) effects of the antimicrobials on the cyanobacterium Synechococcus elongatus and microalga Chlorella vulgaris. The liquid chromatography coupled to mass spectrometry results showed that AZI and IVE were present in all collected samples, while HCQ occurred in 78 % of them. In all the studied sites, the concentrations found of AZI (up to 2.85 µg.L-1) and HCQ (up to 2.97 µg.L-1) represent environmental risks for the studied species, while IVE (up to 3.2 µg.L-1) was a risk only for Chlorella vulgaris. The hazard quotients (HQ) indices demonstrated that the microalga was less sensitive to the drugs than the cyanobacteria. HCQ and IVE had the highest values of HQ for the cyanobacteria and microalga, respectively, being the most toxic drugs for each species. Interactive effects of drugs were observed on growth, photosynthesis and antioxidant activity. The treatment with AZI + IVE resulted in cyanobacteria death, while exposure to the mixture of all three drugs led to decreased growth and photosynthesis in the cells. On the other hand, no effect on growth was observed for C. vulgaris, although photosynthesis has been negatively affected by all treatments. The use of AZI, IVE and HCQ for COVID-19 treatment may have generated surface water contamination, which could increased their potential ecotoxicological effects. This raises the need to further investigation into their effects on aquatic ecosystems.

The phytoremediation capacity of Lemna minor prevents deleterious effects of anti-HIV drugs to nontarget organisms.

We investigated physiological responses of Lemna minor plants and their capacity to remove tenofovir (TNF; 412 ng l-1), lamivudine (LMV; 5428 ng l-1) and/or efavirenz (EFV; 4000 ng l-1) from water through phytoremediation. In addition, the toxicological safety of water contaminated with these drugs after treatment with L. minor plants to photosynthetic microorganisms (Synechococcus elongatus and Chlorococcum infusionum) was evaluated. The tested environmental representative concentrations of drugs did not have a toxic effect on L. minor, and their tolerance mechanisms involved an increase in the activity of P450 and antioxidant enzymes (catalase and ascorbate peroxidase). L. minor accumulated significant quantities of TNF, LMV and EFV from the media (>70%), and the interactive effect of LMV and EFV increased EFV uptake by plants submitted to binary or tertiary mixture of drugs. Photosynthetic microorganisms exposed to TNF + LMV + EFV showed toxicological symptoms which were not observed when exposed to contaminated water previously treated with L. minor. An increased H2O2 concentrations but no oxidative damage in S. elongatus cells exposed to non-contaminated water treated with L. minor was observed. Due to its capacity to tolerate and reclaim anti-HIV drugs, L. minor plants must be considered in phytoremediation programs. They constitute a natural-based solution to decrease environmental contamination by anti-HIV drugs and toxicological effects of these pharmaceuticals to nontarget organisms.

The Role of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Catalase) in the Tolerance of Lemna minor to Antibiotics: Implications for Phytoremediation.

We investigated the individual and combined contributions of two distinct heme proteins namely, ascorbate peroxidase (APX) and catalase (CAT) on the tolerance of Lemna minor plants to antibiotics. For our investigation, we used specific inhibitors of these two H2O2-scavenging enzymes (p-aminophenol, 3-amino,1,2,4-triazole, and salicylic acid). APX activity was central for the tolerance of this aquatic plant to amoxicillin (AMX), whereas CAT activity was important for avoiding oxidative damage when exposed to ciprofloxacin (CIP). Both monitored enzymes had important roles in the tolerance of Lemna minor to erythromycin (ERY). The use of molecular kinetic approaches to detect and increase APX and/or CAT scavenging activities could enhance tolerance, and, therefore, improve the use of L. minor plants to reclaim antibiotics from water bodies.

Crop irrigation (soybean, bean, and corn) with enrofloxacin-contaminated water leads to yield reductions and antibiotic accumulation.

The increasing use of antibiotics in animal production has become an emergent environmental problem. The large percentages of applied antibiotic doses eliminated in animal excrement often end up contaminating water resources, which are then used for irrigation - compromising agricultural production and/or food security. Here, we evaluated the effects of crop irrigation with water artificially contaminated by enrofloxacin (10 µg l-1) and its accumulation in soybean, bean, and corn tissues. Grain production was evaluated on the basis of grain dry weight plant-1, while enrofloxacin and ciprofloxacin (its breakdown metabolite) concentrations in plant tissues were evaluated by HPLC after harvesting. Diminished production was observed only in soybean plants irrigated with antibiotic-contaminated water. Enrofloxacin [1.68 ng g fresh weight (FW)-1 to 26.17 µg g FW-1] and ciprofloxacin (8.23 ng g FW-1 to 51.05 ng g FW-1), were found in all of the plant tissues (roots, leaves, and seeds) of the three species. Regardless of the species, the highest enrofloxacin concentrations were observed in their roots, followed by the leaves and seeds, while ciprofloxacin concentrations varied among the different plant tissues of the different species. The presence of enrofloxacin in the water used for irrigating soybeans can result in productivity losses and, as that antibiotic was encountered in plant tissues (leaves and seeds) of all of the three species analyzed that are consumed in the diets of both humans and animals, it can interfere with food security.

Synergistic effects between arbuscular mycorrhizal fungi and rhizobium isolated from As-contaminated soils on the As-phytoremediation capacity of the tropical woody legume Anadenanthera peregrina.

The synergistic relationship between arbuscular mycorrhizal fungi and plant species may play a key role in phytoremediation of arsenic(As)-contaminated soils. By using modified Leonard jars, we investigated both the distinct and integrative roles of arbuscular mycorrhizal fungi (AMF-Acaulospora scrobiculata) and rhizobia (BH-ICB-A8) isolated from As-contaminated soil on the capacity of Anadenanthera peregrina to reclaim arsenate [As(V)] from soil. AMF inoculation greatly increased plant phosphorous nutrition, as reflected in greater growth, and increased As-concentrations in the roots and shoots. While rhizobia inoculation alone increased nitrogen nutrition it did not promote plant growth or As-uptake. Rhizobia and AMF inoculation together had synergistic effects, however, increasing both the growth and the As-phytoremediation capacity of A. peregrina. Joint inoculation with rhizobia and AMF should therefore be considered a potential technique for rehabilitating As-contaminated areas using A. peregrina.

Emerging contaminants in water used for maize irrigation: Economic and food safety losses associated with ciprofloxacin and glyphosate.

Chemicals used to assure agricultural production and the feasibility of planting sites often end up in bodies of water used for crop irrigation. In a pot study, we investigated the consequences associated with the irrigation of maize with water contaminated by ciprofloxacin (Cipro; 0, 0.2, 0.8, 1.4 and 2.0 µg l-1) and/or glyphosate (0, 5, 25 and 50 mg l-1) on yields and food safety. Glyphosate in concentrations ≥25 mg l-1 prevented plant establishment, regardless of Cipro presence. Evaluations made at the V5 stage of plants reveal that Cipro concentrations ≥0.8 µg l-1 and glyphosate decreased photosynthesis and induced changes in leaf anatomy and stem biophysical properties that may contribute to decreased kernel yields. When those chemicals were applied together, kernel yield reductions were accentuated, evidencing their interactive effects. Irrigation with contaminated water resulted in accumulations of Cipro and glyphosate (as well as its metabolite, aminomethylphosphonic acid) in plant tissues. Accumulation of these chemicals in plant tissues such as leaves and kernels is a problem, since they are used to feed animals and humans. Moreover, these chemicals are of potential toxicological concern, principally due to residue accumulations in the food chain. Specially, the antibiotic residue accumulations in maize tissues can assist the induction of antibiotic resistance in dangerous bacteria. Therefore, we point out the urgency of monitoring the quality of water used for crop irrigation to avoid economic and food-quality losses.

Enrofloxacin and Roundup® interactive effects on the aquatic macrophyte Elodea canadensis physiology.

The co-occurrence of aquatic contaminants, such as antibiotics and herbicides, has motivated investigations into their interactive effects on aquatic organisms. We examined the combined effects of environmental concentrations of the antibiotic Enrofloxacin (Enro; 0-2.25 µg l-1) and Roundup OriginalDI (Roundup®; 0-0.75 µg active ingredient l-1), a glyphosate based-herbicide, on Elodea canadensis. Enro alone was not toxic, but the plants were highly sensitive to Roundup® whose toxicity is related to the induction of oxidative stress. The metabolism of Enro by plants into Ciprofloxacin (Cipro) was observed, and although former is not phytotoxic, oxidative events associated with Cipro generation were observed. The activity of cytochrome P450 was shown to be involved in Enro degradation in E. canadensis. As a cytochrome P450 inhibitor, Roundup® decreases Enro metabolism in plants. Enro, in turn, increases glyphosate uptake and toxicity, so that Enro and Roundup® have synergistic effects, disrupting the physiological processes of E. canadensis. Our results suggest E. canadensis as a potential candidate for the reclamation of Enro in contaminated waters, but not for Roundup® due to its high sensitivity to that herbicide.