RESUMEN
Milk is an important food in the human diet, and copper (Cu) in human milk is indispensable to children's normal growth and development. It is consequently important that Cu deficiency, occurring in malnourished women or in malabsorption following bariatric surgery, be prevented. The objective of this work is to provide hospital-based paediatricians with a tool enabling rapid measurement of Cu in human breast milk through a technique that biology laboratories can easily apply. Using electrothermal atomic absorption spectrophotometry with Zeeman correction, we have optimized this method with two chemical modifiers and without digestion for analytical procedure. Detection limits and quantification limits for Cu in human milk were found to be 0.077 and 0.26 µmol/L, respectively. Within-run (n = 30) and between-run (n = 15) variations in a pool of human milk samples were 1.50 and 3.62%, respectively. Average recoveries ranged from 98.67 to 100.61%. The reliability of this method was also confirmed by analysing certified reference material (10%). In breast milk samples collected from 100 lactating mothers, Cu mean (±1 SD) was 7.09 ± 1.60 µmol/L. In conclusion, with minimal preparation and quick determination, the method proposed is suitable for measurement of Cu in human breast milk.
Asunto(s)
Técnicas de Química Analítica , Cobre/análisis , Leche Humana/química , Espectrofotometría Atómica , HumanosRESUMEN
Aluminium hydroxide is used as an effective adjuvant in a wide range of vaccines for enhancing immune response to the antigen. The pathogenic role of aluminium hydroxide is now recognized by the presence of chronic fatigue syndrome, macrophagic myofasciitis and subcutaneous pseudolymphoma, linked to intramuscular injection of aluminium hydroxide-containing vaccines. The aim of this study is to verify if the subcutaneous pseudolymphoma observed in this patient in the site of vaccine injection is linked to an aluminium overload. Many years after vaccination, a subcutaneous nodule was discovered in a 45-year-old woman with subcutaneous pseudolymphoma. In skin biopsy at the injection site for vaccines, aluminium (Al) deposits are assessed by Morin stain and quantification of Al is performed by Zeeman Electrothermal Atomic Absorption Spectrophotometry. Morin stain shows Al deposits in the macrophages, and Al assays (in µg/g, dry weight) were 768.10±18 for the patient compared with the two control patients, 5.61±0.59 and 9.13±0.057. Given the pathology of this patient and the high Al concentration in skin biopsy, the authors wish to draw attention when using the Al salts known to be particularly effective as adjuvants in single or repeated vaccinations. The possible release of Al may induce other pathologies ascribed to the well-known toxicity of this metal.
Asunto(s)
Hidróxido de Aluminio/toxicidad , Seudolinfoma/diagnóstico , Vacunación/efectos adversos , Aluminio/toxicidad , Biopsia/efectos adversos , Femenino , Humanos , Persona de Mediana Edad , Piel/efectos de los fármacos , Piel/metabolismo , Tejido Subcutáneo/efectos de los fármacos , Tejido Subcutáneo/metabolismoRESUMEN
Aluminum salts such as aluminum chlorohydrate (ACH) are known for use as an active antiperspirant agent that blocks the secretion of sweat. A local case report of hyperaluminemia in a woman using an aluminum-containing antiperspirant for 4 years raises the problem of transdermal absorption of aluminum (Al). Only a very limited number of studies have shown that the skin is an effective barrier to transdermal uptake of Al. In accordance with our analytical procedure, the aim of this study with an in vitro Franz™ diffusion cell was to measure aluminum uptake from three cosmetic formulations of antiperspirant: the base for an "aerosol" (38.5% of ACH), a "roll-on" emulsion (14.5% ACH), and a "stick" (21.2%), by samples of intact and stripped human skin (5 donors). The Al assays were performed by Zeeman Electrothermal Atomic Absorption Spectrophotometry (ZEAAS). Following contacts lasting 6, 12 and 24h, the Al assays showed only insignificant transdermal absorption of Al (≤0.07% of the quantity of Al deposited) and particularly low cutaneous quantities that varied according to the formulations (1.8 µg/cm² for "aerosol base" and "stick" - 0.5 µg/cm² for the "roll-on"). On stripped skin, for which only the "stick" formulation was tested, the measured uptake was significantly higher (11.50 µg/cm² versus 1.81 µg/cm² for normal skin). These results offer reassurance as regards to the use of antiperspirants for topical application of ACH-containing cosmetic formulations on healthy skin over a limited time span (24h). On the other hand, high transdermal Al uptake on stripped skin should compel antiperspirant manufacturers to proceed with the utmost caution.
Asunto(s)
Aluminio/farmacocinética , Antitranspirantes/farmacocinética , Cosméticos/farmacocinética , Piel/metabolismo , Absorción , Adulto , Aluminio/farmacología , Antitranspirantes/farmacología , Cosméticos/farmacología , Emulsiones , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
A local case report of hyperaluminemia (aluminum concentration: 3.88 µmol/L) in a woman using an aluminum-containing antiperspirant for 4 years raises the question of possible transdermal uptake of aluminum salt as a future public health problem. Prior to studying the transdermal uptake of three commercialized cosmetic formulas, an analytical assay of aluminum (Al) in chlorohydrate form (ACH) by Zeeman Electrothermal Atomic Absorption Spectrophotometer (ZEAAS) in a clean room was optimized and validated. This analysis was performed with different media on human skin using a Franz(™) diffusion cell. The detection and quantification limits were set at ≤ 3 µg/L. Precision analysis as within-run (n = 12) and between-run (n = 15-68 days) yield CV ≤ 6%. The high analytic sensitivity (2-3 µg/L) and low variability should allow an in vitro study of the transdermal uptake of ACH.
Asunto(s)
Compuestos de Aluminio/análisis , Antitranspirantes/análisis , Bioensayo/métodos , Piel/química , Bioensayo/instrumentación , Bioensayo/normas , Biopsia , Calibración , Cámaras de Difusión de Cultivos , Humanos , Límite de Detección , Reproducibilidad de los Resultados , Espectrofotometría AtómicaRESUMEN
The putative role of aluminium intake in young Bangladeshi children (1.5 to 4 years of age) with calcium-deficient rickets was evaluated in a non randomised controlled eight month trial. The effects of aluminium or stainless-steel cooking pots on bone metabolism were assessed by measuring blood calcium, phosphorus, alkaline phosphatase, parathyroid hormone, 1,25 dihydroxy vitamin D, aminoterminal propeptide of type 1 collagen (PINP), cross-linked carboxyterminal telopeptide of type 1 collagen (ICTP), aluminium and albumin, and by analysis of wrist radiographs. In both groups, blood alkaline phosphatase, 1,25 dihydroxy vitamin D and aluminium decreased significantly, white serum albumin increased (p < 0.01). These results suggest that the nutrition may well be of major importance, whereas the role of aluminium appears to be insignificant.