Use of Pseudomonas protegens to Control Root Rot Disease Caused by Boeremia exigua var. exigua in Industrial Chicory (Cichorium intybus var. sativum Bisch.).

Boeremia exigua var. exigua is a recurrent pathogen causing root rot in industrial chicory. Currently, there is no chemical or varietal control for this disease, and thus, management strategies need to be developed. This study determined the biocontrol effect of strains of Pseudomonas protegens bacteria with antimicrobial compounds on the fungus B. exigua var. exigua under in vitro, in vivo, and field conditions. In addition, root colonization by these bacteria was estimated by the phlD-specific PCR-based dilution end point assay. Eighteen isolates of Pseudomonas spp were evaluated, and the strains that showed the greatest in vitro inhibition of fungal mycelial growth (mm), Ca10A and ChB7, were selected. Inoculation with the strain ChB7 showed less severity (necrotic area) under in vivo conditions (root trials) compared with the control inoculated with the pathogen (p ≤ 0.05). The molecular analysis revealed that the root colonization of plants grown in pots was equal to or greater than 70%. Similar levels were observed in the field trials conducted at the Selva Negra and Canteras experimental stations (2015-2016 season), with values ranging from 85.7 to 70.5% and from 75.0 to 79.5%, respectively. Regarding yield (ton ha-1), values were higher in the treatments inoculated with strains Ca10A and ChB7 (p ≤ 0.05) at both experimental sites, while a lower incidence and severity of root rot were observed at Selva Negra. These results suggest that the Chilean strains of P. protegens are a promising tool for the control of root diseases in industrial chicory.

Adaptación y validación de la Escala de Dificultades en la Regulación Emocional en adultos del Área Metropolitana de Buenos Aires / Adaptation and Validation of the Emotional Regulation Difficulty Scale in Adults in the Metropolitan Area of Buenos Aires / Adaptação e validação da Escala de Dificuldades na Regulação Emocional em adultos da Área Metropolitana de Buenos Aires

La desregulación emocional (de) fue identificada como uno de los principales procesos involucrados en el surgimiento y mantenimiento de los trastornos emocionales. A su vez, los trastornos emocionales son los más prevalentes mundialmente, volviendo a la deun objeto de estudio de gran relevancia. El presente trabajo consiste en la adaptación y validación de la Escala de Dificultades en la Regulación Emocional (Difficulties in Emotion Regulation Scale, ders) (Gratz & Roemer, 2004), para ser usada en población general del Área Metropolitana de Buenos Aires. Se realizó un estudio transversal con 315 participantes entre 18 y 65 años. La consistencia interna resultó adecuada (α = 0.936; ω = 0.938). Se llevó a cabo un análisis factorial confirmatorio mediante el paquete Lavaan, quedando la escala conformada por 30 ítems que se ajustan a las seis dimensiones originales. También se presenta evidencia de validez convergente y discriminante, la capacidad de discriminación de los ítems, diferencias por sexo y edad, y valores normativos para adultos del Área Metropolitana de Buenos Aires. La adaptación al español de la ders representa una herramienta válida y confiable para medir distintos aspectos del proceso de regulación emocional

Emotional dysregulation (ed) was identified as one of the main processes involved in the emergence and maintenance of emotional disorders. In turn, emotional disorders are the most prevalent worldwide, making ed an object of study of great relevance.The present study consists of the adaptation and validation of the Difficulties in Emotion Regulation Scale (ders) (Gratz & Roemer, 2004) for its use in the general population of the Buenos Aires Metropolitan Area. A cross-sectional study was conducted with 315 participants between 18 and 65 years old. Internal consistency was adequate (α = 0.936; ω = 0.938). A confirmatory factor analysis was performed using the Lavaan package, leaving the scale with 30 items which fit the six original dimensions. There is also evidence of convergent and discriminant validity, the items' discrimination ability, differences by sex and age, and normative values for adults in the Metropolitan Area of Buenos Aires. The Spanish adaptation of the ders represents a valid and reliable tool to measure different aspects of the emotional regulation process.

A desregulação emocional (de) foi identificada como um dos principais processos envolvidos no surgimento e manutenção dos transtornos emocionais. Por sua vez, os transtornos emocionais são os mais prevalentes no mundo, tornando a de um objeto de estudo de grande relevância. O presente estudo consiste na adaptação e validação da Escala de Dificuldades na Regulação Emocional (ders) (Gratz & Roemer, 2004) para sua utilização na população geral da região metropolitana de Buenos Aires. Foi realizado um estudo transver-sal com 315 participantes com idade entre 18 e 65 anos. A consistência interna foi adequada (α = 0.936; ω = 0.938). Realizou-se uma análise fatorial confirmatória utilizando o pacote Lavaan, deixando a escala com há também evidências de validade convergente e discriminante, capacidade de discriminação dos itens, diferenças por sexo e idade, e valores norma-tivos para adultos na Área Metropolitana de Buenos Aires. A adaptação espanhola do ders representa uma ferramenta válida e confiável para mensurar diferentes aspectos do processo de regulação emocional.

Identification of RP1 as the genetic cause of retinitis pigmentosa in a multi-generational pedigree using Extremely Low-Coverage Whole Genome Sequencing (XLC-WGS).

MOTIVATION: Next-generation sequencing (NGS) technologies are decisive for discovering disease-causing variants, although their cost limits their utility in a clinical setting. A cost-mitigating alternative is an extremely low coverage whole-genome sequencing (XLC-WGS). We investigated its use to identify causal variants within a multi-generational pedigree of individuals with retinitis pigmentosa (RP). Causing progressive vision loss, RP is a group of genetically heterogeneous eye disorders with approximately 60 known causal genes. RESULTS: We performed XLC-WGS in seventeen members of this pedigree, including three individuals with a confirmed diagnosis of RP. Sequencing data were processed using Illumina's DRAGEN pipeline and filtered using Illumina's genotype quality score metric (GQX). The resulting variants were analyzed using Expert Variant Interpreter (eVai) from enGenome as a prioritization tool. A nonsense known mutation (c.1625C > G; p.Ser542*) in exon 4 of the RP1 gene emerged as the most likely causal variant. We identified two homozygous carriers of this variant among the three sequenced RP cases and three heterozygous individuals with sufficient coverage of the RP1 locus. Our data show the utility of combining pedigree information with XLC-WGS as a cost-effective approach to identify disease-causing variants.

Manejo de discontinuidad pélvica debido a fractura de acetábulo mediante cirugía de revisión de artroplastia de cadera y reemplazo de componente acetabular por anillo de Burch-Schneider: reporte de un caso / Treatment for pelvic discontinuity due to acetabular fracture by means of hip arthroplasty revision surgery and acetabular component replacement using a Burch-Schneider ring: a case report

Introducción. La discontinuidad pélvica es una complicación de la artroplastia total de cadera que consiste en la separación de la hemipelvis superior e inferior a través del acetábulo.Presentación del caso. Hombre de 74 años con antecedente de artroplastia total de cadera izquierda que requirió 4 cirugías de revisión de artroplastia, quien consultó al servicio de urgencias de un hospital de segundo nivel de atención de Sogamoso (Colombia) por dolor y limitación de la movilidad de la cadera izquierda durante 7 días luego de haber sufrido trauma por caída de su propia altura en la que el miembro inferior izquierdo recibió la fuerza del impacto. En el examen físico, se evidenció limitación de los arcos de movilidad de la cadera izquierda, por lo que se realizó una radiografía de cadera, en la que se observó fractura del acetábulo, y una tomografía computarizada de cadera, en la que se evidenciaron signos de aflojamiento del componente acetabular y fractura del acetábulo (tipo IIIB según clasificación de Paprosky). Teniendo en cuenta lo anterior, se diagnosticó discontinuidad pélvica y se realizó cirugía de revisión de artroplastia y reemplazo del componente acetabular por un anillo de Burch-Schneider. El paciente tuvo una adecuada evolución posoperatoria con seguimiento a 3 meses. Conclusión. Si bien hay múltiples opciones para el manejo de la discontinuidad pélvica, no hay consenso sobre cuál es la mejor; sin embargo, el uso de componentes acetabulares de refuerzo como el anillo de Burch-Schneider fue una opción con buenos resultados posoperatorios en este caso

Introduction: Pelvic discontinuity is a complication of total hip arthroplasty, consisting of a structural bone defect of the acetabulum with separation of the upper and lower hemipelvis. Case presentation: A 74-year-old male patient with a history of total left hip arthroplasty and 4 arthroplasty revision surgeries visited the emergency department of a secondary care hospital due to pain and limited mobility of the left hip over a period of 7 days after having suffered a fall from his own height in which the left lower limb received the force of the impact. Physical examination showed limitation of the range of motion of the left hip, so a hip X-ray was performed, showing a fracture of the acetabulum. A computed tomography of the hip revealed signs of loosening of the acetabular component and fracture of the acetabulum (type IIIB according to the Paprosky classification). In view of the above, pelvic discontinuity was diagnosed and revision arthroplasty and replacement of the acetabular component with a Burch-Schneider ring was performed. The patient had an adequate postoperative progression and follow-up at 3 months.Conclusion: Although there are multiple options for the treatment of pelvic discontinuity, currently, there is no conclusive data regarding the best therapeutic option. However, the use of reinforcement acetabular components, such as the Burch-Schneider ring, was an option with good postoperative outcomes in this case

Las concepciones morales de José Martí acerca de la Educación Física, el Deporte y la Recreación / The moral conceptions of José Martí about Physical Education, Sports and Recreation / As concepções morais de José Martí sobre Educação Física, Esportes e Recreação

RESUMEN Introducción: José Martí, héroe nacional de Cuba, ha legado con el paso del tiempo, diversas ópticas progresistas, inclusive en el deporte, una faceta menos conocida en su fecunda labor periodística y literaria. Se conoce que practicó una sola especialidad: el ajedrez; pero en sus crónicas demostró conocimientos sobre carreras de fondo, fútbol americano, billar, boxeo, equitación, esgrima, caza, corridas de toros y patinaje, entre otros. Objetivo: Profundizar en las concepciones morales del Héroe Nacional de Cuba acerca del deporte y la Educación Física. Materiales y métodos: Para llevar a cabo este estudio se desarrolló una revisión bibliográfica de las obras completas de José Martí, referidas a las escenas norteamericanas donde se recoge en mayor medida las crónicas martianas acerca del deporte y la Educación Física. Resultados: El tema que se aborda por su interés trasciende las fronteras de su época y se inserta en el proyecto de las ideas que sustentan a la revolución cubana, de que el deporte es un derecho del pueblo; a ello va dedicado el presente artículo, a resaltar las opiniones que vertió José Martí sobre el deporte, la Educación Física y la Recreación, siendo un crítico de estos desde el punto de vista moral. Conclusiones: José Martí elaboró un arsenal de ideas que constituyen un alto aporte al desarrollo del deporte y la Educación Física. En el periodismo el maestro tuvo una de las visiones más certeras sobre la práctica del deporte.

SÍNTESE Introdução: José Martí, o herói nacional de Cuba, legou com o passar do tempo, várias visões progressistas, inclusive no esporte, uma faceta menos conhecida de sua prolífica obra jornalística e literária. Sabe-se que ele praticou apenas uma especialidade: xadrez; mas em suas crônicas ele demonstrou seus conhecimentos de corrida de longa distância, futebol americano, bilhar, boxe, equitação, esgrima, caça, toureio e patinação, entre outros. Objetivo: Estudar em profundidade as concepções morais do Herói Nacional de Cuba sobre esporte e Educação Física. Método: a fim de realizar este estudo, foi realizada uma revisão bibliográfica dos trabalhos completos de José Martí, referindo-se às cenas norte-americanas onde as crônicas de Marti sobre esporte e Educação Física são coletadas em maior escala. Resultados: O assunto tratado por seu interesse transcende as fronteiras de seu tempo e está inserido no projeto das idéias que sustentam a revolução cubana, que o esporte é um direito do povo; é a isso que este artigo é dedicado, para destacar as opiniões que José Martí expressou sobre esporte, Educação Física e Recreação, sendo um crítico destes do ponto de vista moral. Conclusões: José Martí elaborou um arsenal de idéias que constituem uma grande contribuição para o desenvolvimento do esporte e da Educação Física. No jornalismo, o professor tinha uma das visões mais precisas sobre a prática do esporte.

ABSTRACT Introduction: José Martí, Cuba's national hero, has bequeathed various progressive perspectives over time, including in sports, a lesser-known facet of his prolific journalistic and literary work. It is known that he practiced a single specialty: chess; but in his chronicles he demonstrated knowledge of long-distance running, American football, billiards, boxing, horse riding, fencing, hunting, bullfighting and skating, among others. Objective: To deepen the moral conceptions of the National Hero of Cuba about sports and Physical Education. Materials and methods: to carry out this study, a bibliographic review of the complete works of José Martí was developed, referring to the North American scenes where Martí's chronicles about sports and Physical Education are collected to a greater extent. Results: the topic that is addressed by his interest transcends the borders of his time and is inserted in the project of the ideas that sustain the Cuban revolution, that sport is a right of the people; This article is dedicated to this, to highlight the opinions expressed by José Martí on sports, Physical Education and Recreation, being a critic of these from the moral point of view. Conclusions: José Martí developed an arsenal of ideas that constitute a high contribution to the development of sport and Physical Education. In journalism, the teacher had one of the most accurate views on the practice of sport.

Effectiveness and adherence to closed face shields in the prevention of COVID-19 transmission: a non-inferiority randomized controlled trial in a middle-income setting (COVPROSHIELD).

BACKGROUND: The use of respiratory devices can mitigate the spread of diseases such as COVID-19 in community settings. We aimed to determine the effectiveness of closed face shields with surgical face masks to prevent SARS-CoV-2 transmission in working adults during the COVID-19 pandemic in Bogotá, Colombia. METHODS: An open-label non-inferiority randomized controlled trial that randomly assigned participants to one of two groups: the intervention group was instructed to wear closed face shields with surgical face masks, and the active control group was instructed to wear only surgical face masks. The primary outcome was a positive reverse transcription polymerase chain reaction test, IgG/IgM antibody test for SARS-CoV-2 detection, or both during and at the end of the follow-up period of 21 days. The non-inferiority limit was established at - 5%. RESULTS: A total of 316 participants were randomized, 160 participants were assigned to the intervention group and 156 to the active control group. In total, 141 (88.1%) participants in the intervention group and 142 (91.0%) in the active control group completed the follow-up. PRIMARY OUTCOME: a positive SARS-CoV-2 test result was identified in one (0.71%) participant in the intervention group and three (2.1%) in the active control group. In the intention-to-treat analysis, the absolute risk difference was - 1.40% (95% CI [- 4.14%, 1.33%]), and in the per-protocol analysis, the risk difference was - 1.40% (95% CI [- 4.20, 1.40]), indicating non-inferiority of the closed face shield plus face mask (did not cross the non-inferiority limit). CONCLUSIONS: The use of closed face shields and surgical face masks was non-inferior to the surgical face mask alone in the prevention of SARS-CoV-2 infection in highly exposed groups. Settings with highly active viral transmission and conditions such as poor ventilation, crowding, and high mobility due to occupation may benefit from the combined use of masks and closed face shields to mitigate SARS-CoV-2 transmission. TRIAL REGISTRATION: ClinicalTrials.gov NCT04647305 . Registered on November 30, 2020.

Prevalence and C-shaped root canal configuration in lower molars in the metropolitan region, Chile / Prevalencia y configuración de conductos en C en molares inferiores de la región metropolitana, Chile

Objetive: The aim of this study was to determine the prevalence, demographics, and root configuration of C-shaped canals of mandibular molars by means of cone beam computed tomography in the population of the Metropolitan Region, Chile. Material and Methods: 912 molars (456 first and 456 second molars) resulting from the analysis of 228 mandibular CT scans were evaluated. The root configuration was established by means of a panoramic reconstruction and axial tomographic sections, classifying the presence and type of canals through the analysis of five sections or cuts along the root. Data were statistically analyzed using a 5% confidence interval. Results: Of the 912 molars analyzed, 70 were classified as C-shaped canals (7.68%), corresponding to 58.33% of those molars that presented fused roots. 95.7% of this root canal configuration was observed in lower second molars, occurring more frequently in females (n=45, 64.29%). 45.65% of the cases that presented C-shaped canals were bilateral and the most frequent configuration was C3 (n=401, 66.63%) according to the Melton classification.Conclusion: The C-shaped canals of the mandibular molars in the studied population were observed mainly in second molars, showing a clear prevalence among females and a high percentage of bilaterality. The presence of fused roots significantly increases the possibility of finding this type of root configuration.

Objetivo: El objetivo del presente estudio fue determinar, por medio de tomografía computarizada de haz cónico, la prevalencia, demografía y configuración radicular de los conductos en forma de C de molares inferiores en la población de la Región Metropolitana, Chile. Material y Métodos: 912 molares (456 primeros y 456 segundos molares) resultantes del análisis de 228 tomografías mandibulares fueron evaluados. Mediante una reconstrucción panorámica y cortes tomográficos axiales se estableció la configuración radicular, clasificando la presencia y tipo de conducto por medio del análisis de 5 cortes a lo largo de la raíz. Los datos fueron analizados estadísticamente con un intervalo de confianza del 5%. Resultados: De los 912 molares analizados, 70 fueron clasificados como conductos en forma de C (7.68%), correspondiendo al 58.33% de aquellos molares que presentaron raíces fusionadas. El 95.7% de esta configuración de conductos fue observada en segundos molares inferiores, presentándose con mayor frecuencia en mujeres (n=45, 64.29%). El 45.65% de los casos que presentaron conductos en C se manifestaron bilateralmente y la configuración más frecuente observada fue la C3 (n=401, 66.63%) según la clasificación de Melton. Conclusión: Los conductos en forma de C de los molares mandibulares en la población estudiada fueron observados principalmente en segundos molares, marcando una clara predilección por el sexo femenino y un alto porcentaje de bilateralidad. La presencia de raíces fusionadas aumenta significativamente la posibilidad de encontrar este tipo de configuración radicular.

First report of Diplodia mutila causing wood necrosis in European hazelnut in Chile.

In Chile, the planted area of European hazelnut (Corylus avellana L.) reaches around 30,000 hectares, mainly concentrated in the central and southern area of the country where climate and soil provide a natural environment well suited to growing this species. Only a few diseases affect this nut tree in Chile. During the spring seasons in 2018 and 2020, European hazelnut plants (6 to 20% of incidence) exhibited wood necrosis and vascular discoloration of branches, with reduced growth, cankers and wilt branches, in orchards located in San Clemente and Curicó, Maule Region, Bulnes and El Carmen, Ñuble Region, Chile (36°45'-36°54' S; 71°03'-72°26' W). Symptomatic tissues were surface disinfected using a ~1% commercial sodium hypochlorite solution. Disinfected tissues were cut longitudinally, placed onto potato dextrose agar (PDA, Difco) plates, and incubated at 25 °C in the dark for 48 hours. Fungal hyphal tips were taken and placed on PDA medium. A fungal species was consistently isolated from these lignified tissues. The mycelium was initially translucent (turning white in appearance), while the mature mycelium was aerial, varying in color from pale to dark gray (Munsell color code: colony edge mycelium 6Y-6 4 / 5G and colony center mycelium B6-PB 7 / 5PB). The production of pycnidia and conidia was induced using pine needles in water agar medium and incubated in the dark for 10 days. Hyaline unicellular conidia of 25 ± 1.1 µm (range 23.9 to 26.1 µm) long and 11 ± 0.5 µm (Range 10.5 to 11.5 µm) wide (n = 50) were obtained from black pycnidia. Based on the cultural and morphological characteristics observed, the pathogen was identified as a possible species of the family Botryosphaeriaceae (20 isolates). Molecular techniques were used to identify the species of pathogen, and three isolates (F154, F199, and F167) were analyzed by using Multilocus sequence typing to confirm the identity of the pathogen. Genes ITS (internal transcribed spacer region), tef-1 (translation elongation factor 1-alpha) and ß-tub (ß-tubulin) were amplified using endpoint PCR, with primers ITS1/ITS4 (White et al., 1990), EF1-728F/EF1-986R (Carbone & Kohn, 1999) and Bt2a/Bt2b (Glass & Donaldson, 1995), respectively. The segments were sequenced using the same primers, deposited in Gen Bank, and the accession numbers for each isolate were OM993582, OM993583, ON003481 for ITS, ON054936, ON054938, ON054937 for tef1 and ON054939, ON054941, ON054940 for ß-tub, respectively. A phylogenetic tree was constructed using the maximum likelihood statistical method with the Tamura-Nei model based on a concatenated dataset of ITS region, tef1 and ß-tubulin gene using Mega-X, and the three Chilean isolates (F154, F199, and F167) formed a single clade with the reference isolates of Diplodia mutila (Fr.) Mont. BLAST algorithm analyses indicated 100% identity to D. mutila for ITS (accession NR_144906), for tef-1 (accession MK573559), and for ß-tubulin (accession MG952719). The pathogenicity of the three isolates was validated through Koch's postulates. For this purpose, a trial was established in 6-year-old European hazelnut plants cv. Tonda Di Giffoni. Ten healthy branches were individually inoculated using actively growing mycelial discs from each isolate, while a disc of PDA without fungus was used as a control. Holes of 5-mm diameter were inoculated, making sure the mycelium was in contact with the wood. Finally, the wounds were sealed with plastic film to prevent external contamination and improve humidity conditions. After 120 days, each branch was cut longitudinal-sectioned to verify the presence of wood necrosis which arose between 3.0 to 16.2 mm of length around the point of inoculation. No necrosis was observed in the control. To confirm pathogenicity, infected tissues were cut into small pieces with sterile knives and scalpels, and surface disinfected with a 1% sodium hypochlorite solution for 1 min. The disinfected tissues were placed on PDA medium and incubated at 25°C in the dark until fungal growth was observed. Hyphal tips were taken from the mycelia developed from the pieces of wood, and placed on PDA medium in order to obtain pure isolates. The pathogenicity of the D. mutila isolates F154 and F199 was observed in 100% of the inoculated branches, while isolate F167 showed symptoms in 85% of the branches. The reisolated strains showed similar mycelial growth and microscopic fungal structures to those observed in the isolates used for inoculation. This is the first report of D. mutila affecting European hazelnut in Chile. This fungus has been recently reported affecting hazelnut in Oregon, USA (Wiman et al., 2019), causing similar symptoms to those observed in our study. In addition, D. mutila has been reported infecting walnut in Chile (Diaz et al. 2018) and native forest trees, specifically Araucaria araucana in Chile (Besoain et al., 2017). The presence of D. mutila in commercial hazelnut orchards in Chile highlights the need for epidemiological studies in order to understand the characteristics and impact of this pathogen and, based on this, develop adequate phytosanitary programs for its control.

Contemporaneous Emotion Regulation Theoretical Models: A Systematic Review / Modelos Teóricos Contemporáneos de Regulación Emocional: Una Revisión Sistemática

ABSTRACT Background: Emotions and their regulation are a phenomenon present in everyday life. Despite its relevance and growing interest, a consensual and univocal definition has not yet been reached. Objective: This paper aims to review contemporary theoretical models of emotion regulation, looking for agreements and divergences between authors. It seeks to identify the main processes considered when working with emotional dysregulation. Method: Our team conducted a systematic review in the form of a narrative synthesis following the guidelines of the PRISMA statement. The database used were SCOPUS, PUBMED, and Dialnet. We included articles published between 2018 and 2020, which have been peer-reviewed in indexed scientific journals, whose central theme was the theoretical presentation of the construct of emotional regulation. We excluded articles that conceptualized only one dimension of the construct focused on specific populations and empirical studies without a theoretical conceptualization of the construct. The information was systematized in a table identifying authors' information, country of institutional affiliation, main characteristics of the given definition of emotion regulation, regulation skills mentioned, and underlying theoretical frameworks. Results: We identified ten different theoretical frameworks that propose models of emotion regulation. The main components found in the definition were the complexity of the construct, goal orientation, intra- or interpersonal regulation, the proposal of moderators, and its voluntary character. Discussion: There is a consensus on the use of emotion regulation strategies to adapt to environmental demands, achieve goals and increase well-being. We identify that people's learning history is an important factor in the development of emotional regulation skills. In addition, context and personality traits are proposed as moderators of the therapeutic efficacy of interventions focused on emotional regulation. Further studies along these lines would favor the implementation of preventive interventions and the personalization of treatments.

RESUMEN Antecedentes: Las emociones y su regulación son un fenómeno presente en la vida cotidiana. A pesar de su relevancia y creciente interés, aún no se ha alcanzado una definición consensuada y unívoca. Objetivo: El presente trabajo pretende revisar los modelos teóricos de regulación emocional contemporáneos buscando acuerdos y divergencias entre autores. Se busca identificar los principales procesos tomados en cuenta para el trabajo con la desregulación emocional. Método: Nuestro equipo realizó una revisión sistemática en forma de síntesis narrativa siguiendo las directrices de PRISMA. Las bases de datos utilizadas fueron SCOPUS, PUBMED y Dialnet. Se incluyeron artículos publicados entre 2018 y 2020, que han sido revisados por pares en revistas científicas indexadas, cuyo tema central fuera la presentación teórica del constructo de regulación emocional. Se excluyeron artículos que conceptualizaban una sola dimensión del constructo, se enfocaban en poblaciones específicas y estudios empíricos sin una conceptualización teórica del constructo. La información fue sistematizada en una tabla identificando información de los autores, país de afiliación institucional, características principales de la definición dada de regulación emocional, habilidades de regulación mencionadas y marco teórico de base. Resultados: Se identificaron diez marcos teóricos diferentes que proponen modelos de regulación de las emociones. Los principales componentes encontrados en las definiciones fueron la complejidad del constructo, la orientación a metas, la regulación intra o interpersonal, la propuesta de moderadores y su carácter voluntario. Discusión: Existe un consenso sobre el uso de estrategias de regulación de las emociones para adaptarse a las demandas del entorno, alcanzar metas y aumentar el bienestar. Identificamos que la historia de aprendizaje de las personas es un factor importante en el desarrollo de las habilidades de regulación emocional. Además, el contexto y los rasgos de personalidad son propuestos como moderadores de la eficacia terapéutica de las intervenciones centradas en la regulación emocional. Más estudios en esta línea favorecerían la implementación de intervenciones preventivas y la personalización de los tratamientos.

The First Cathedral on America's Pacific Coast.

New research dispels the idea that Panamá Viejo was initially founded one-half mile from the site of its visible present-day ruins. The archaeological and historical evidence, subjected to interdisciplinary analysis, demonstrates that the city remained on the same main plaza next to its natural port from its founding 500 years ago until its destruction in 1671. The data reconsidered and newly uncovered also suggest reasons for previous misinterpretations of the city's early foundational history. Unlike many colonial cities and towns, Panamá Viejo did not move during its first century of existence. However, its main church, which became the bishopric's cathedral in 1524, did relocate after 1541. The new evidence establishes and confirms the original location of the first cathedral on America's Pacific Ocean to the south of Panamá Viejo's main plaza and explains its move to an elevated, rocky area on the eastern side of the same plaza over 20 years later. Excavations undertaken in 2018 have confirmed the original building's location a mere 50 m from the visible ruins of the cathedral, the tower of which remains a symbol of Panamanian identity today.

Las nuevas investigaciones despejan la idea de que Panamá Viejo fue inicialmente fundada a milla y media del sitio en donde ahora son visibles sus ruinas. Las evidencias arqueológicas e históricas, sometidas a un análisis interdisciplinar, demuestran que la ciudad permaneció en el mismo emplazamiento, al lado de su puerto natural, fundada 500 años atrás hasta su destrucción en 1671. La revisión de la información disponible, además de la descubierta recientemente, también sugieren las razones de los errores interpretativos previos en torno a la historia fundacional de la ciudad. A diferencia de numerosas ciudades y pueblos coloniales, Panamá Viejo no fue trasladada durante su primer siglo de existencia. Sin embargo, su iglesia principal, la cual se convirtió en sede catedralicia en 1524, fue reubicada después de 1541. La nueva evidencia confirma la localización original de la primera catedral en el Pacífico americano al sur de la plaza mayor de Panamá Viejo y explica el traslado a un área rocosa más elevada, en el costado este de esta plaza, unos veinte años más tarde. Las excavaciones adelantadas en el 2018 han confirmado su ubicación original a escasos 50 m de las actuales ruinas de la catedral, cuya torre permanece como un símbolo de la identidad panameña hoy.

Une nouvelle recherche écarte l'idée que la fondation de Panama Viejo soit intervenue originellement à 800 m du site de ses ruines visibles de nos jours. Les preuves historiques et archéologiques, lorsqu'elles sont assujetties à une analyse interdisciplinaire, démontrent que la ville est demeurée sur la même place principale que son port naturel à compter de sa fondation il y a 500 ans jusqu'à sa destruction en 1671. Les données ayant fait l'objet d'un nouvel examen et qui ont été découvertes suggèrent également les motifs à l'appui des interprétations erronées de l'histoire de la fondation initiale de la ville. Contrairement à de nombreuses villes et cités coloniales, Panama Viejo ne s'est pas déplacée au cours du premier siècle de son existence. Cependant, son église principale qui est devenue en 1524 la cathédrale de l'évêché, s'est implantée ailleurs après 1541. Les preuves nouvelles établissent et confirment le site originel de la première cathédrale sur l'Océan pacifique de l'Amérique au sud de la place principale de Panama Viejo et expliquent son déplacement vers une zone élevée, rocheuse sur la partie orientale de la même place plus de 20 ans par la suite. Les fouilles entreprises en 2018 ont confirmé le site de la bâtisse d'origine à seulement 50 m des ruines visibles de la cathédrale, dont la tour demeure un symbole de l'identité panaméenne de nos jours.

Stump appendicitis after laparoscopic appendectomy; laparoscopic management and literature review.

INTRODUCTION: Appendectomy is one of the most common surgical procedures performed worldwide There are different etiologies for acute appendicitis such as obstruction of the appendiceal lumen by fecalith, lymphoid hyperplasia, or neoplasm. Laparoscopic appendectomy has become the treatment of choice for both complicated and uncomplicated appendicitis; common postoperative complications include wound infection, bleeding, intraabdominal abscess. Stump appendicitis is defined as the interval repeated inflammation of remaining residual appendiceal tissue after an appendectomy. PRESENTATION OF CASE: 38-Year-old female patient with a history of laparoscopic appendectomy performed in 2016 for acute uncomplicated appendicitis. She arrived to the emergency room due to abdominal pain 7 out of 10, located in the periumbilical region, later with migration to the right lower quadrant, abdominal CT scan evidenced the presence of a cecal and pericecal inflammatory process as well as the base and residual proximal portion of the cecal appendix laparoscopic stump appendectomy was performed. DISCUSSION: Stump appendicitis (SA) is defined as the inflammation of the remnant of the cecal appendix after an appendectomy, whether due to impaction of a fecalith or secondary to an ischemic process, the probability of developing SA is estimated to be about 1/50,000 cases throughout life. The most frequently used treatment is exploratory laparotomy to complete the previous appendectomy; however, there are 5 reported cases of stump appendicitis, where surgical resolution was performed through laparoscopic surgery. CONCLUSIONS: It is important to keep this entity in mind when evaluating a patient with acute abdomen with previous history of appendectomy, since the delay in diagnosis and treatment increases morbidity and mortality; laparoscopic stump appendectomy has been shown to be a safe treatment (Agha et al., 2020 [14]).1.

Archaeogenomic distinctiveness of the Isthmo-Colombian area.

The recently enriched genomic history of Indigenous groups in the Americas is still meager concerning continental Central America. Here, we report ten pre-Hispanic (plus two early colonial) genomes and 84 genome-wide profiles from seven groups presently living in Panama. Our analyses reveal that pre-Hispanic demographic events contributed to the extensive genetic structure currently seen in the area, which is also characterized by a distinctive Isthmo-Colombian Indigenous component. This component drives these populations on a specific variability axis and derives from the local admixture of different ancestries of northern North American origin(s). Two of these ancestries were differentially associated to Pleistocene Indigenous groups that also moved into South America, leaving heterogenous genetic footprints. An additional Pleistocene ancestry was brought by a still unsampled population of the Isthmus (UPopI) that remained restricted to the Isthmian area, expanded locally during the early Holocene, and left genomic traces up to the present day.

Argentinian Mental Health During the COVID-19 Pandemic: A Screening Study of the General Population During Two Periods of Quarantine.

Background: Due to the COVID-19 pandemic, Argentina has been under mandatory quarantine. We have aimed to investigate the state of mental health of the Argentine population and the behaviours adopted to cope with mental distress during quarantine. Method: An online survey was conducted using a probabilistic sampling technique and stratified according to the geographic regions of the country. The survey covered days 7-11 (n = 2,631) and days 50-55 (n = 2,068) after compulsory quarantine. The psychological impact was measured using the 27-item Symptom CheckList (SCL-27), which provides a Global Severity Index (GSI). An ad hoc questionnaire registered problematic, healthy and other behaviours. Two network models were estimated using a Mixed Graphical Model. Data from the two periods were compared and analysed. Outcomes: Higher GSI scores and greater risk of experiencing mental disorder were found in Period 2 as compared with Period 1. The lowest GSI scores were associated with physical activity in both periods, and meditation and yoga in Period 1. Drug users reported the highest GSI scores in both periods. The Network Comparison Test confirmed a significant change in symptomatology structure over the two quarantine periods. Conclusion: This study showed that psychological symptoms and the risk of experiencing mental disorder increased significantly from Period 1 to Period 2. Network analysis suggested that the quarantine might have brought about changes in the relationships between symptoms. Overall results revealed the relevance of mental health and the need to take mental health actions upon imposing quarantine during the current COVID-19 pandemic.

La exitosa respuesta de los consolareños a la COVID-19 en la comunidad Camilo Cienfuegos / The successful response of Consolacion del Sur inhabitants to COVID-19 in Camilo Cienfuegos community

RESUMEN Introducción: la aparición de la pandemia de la COVID-19 trajo como resultado que más de 180 países estén afectados. Cuba no ha estado ajena a esta situación. El primer evento epidemiológico registrado en la comunidad Camilo Cienfuegos, municipio Consolación del Sur, provincia Pinar del Río, que puso a prueba la capacidad de movilización del personal médico, y de los sectores que de una u otra forma colaboraron en la puesta en cuarentena entre el 31 de marzo y el 1º de mayo, para evitar la propagación. Objetivo: caracterizar el comportamiento de la comunidad Camilo Cienfuegos durante la cuarentena por la COVID-19. Métodos: se utilizaron métodos empíricos, la observación, revisión documental, entrevistas a miembros del equipo médico y a dirigentes de la comunidad. Desarrollo: se logró una caracterización de la comunidad en el proceso de la cuarentena a partir de su indicación por el Consejo de Defensa Municipal, para el enfrentamiento y la prevención del surgimiento de nuevos casos de la enfermedad. Conclusiones: la caracterización histórica de la comunidad Camilo Cienfuegos permitió al Consejo de Defensa Municipal disponer de una herramienta testimonial sobre el tratamiento de la COVID-19, lo que facilitó su aplicación o consulta a posteriores situaciones episódicas de la enfermedad en la provincia y el país. Así como su importancia para la toma de decisiones en el enfrentamiento a la pandemia.

ABSTRACT Introduction: the emergence of Covid-19 pandemic resulted in more than 180 countries being affected today. Cuba has not been exempt from this situation and its first epidemiological event took place in Camilo Cienfuegos community, Consolacion del Sur municipality, Pinar del Río province, which tested the mobilization capacity of the medical personnel, and of the sectors that in one way or another collaborated in the period of quarantine between March 31 and May 1, in order to avoid the spread and the onset of new cases. Objective: to characterize the behavior of Camilo Cienfuegos community during Covid-19 period of quarantine. Methods: empirical methods such as observation, documentary review and interviews with members of the medical team and community leaders were applied. Development: a characterization of the community in the quarantine process was accomplished complying with the indications of the Municipal Defense Council to cope with and prevent the onset of Covid-19 new cases. Conclusions: the historical characterization of Camilo Cienfuegos community allowed the Municipal Defense Council to have a testimonial tool on the treatment of Covid-19, which facilitated the further requests or enquiries of posterior episodic situations of this disease in the province and the country, as well as its importance for decision making in coping with this pandemic.

Benefits of multidisciplinary team management of obese patients with intragastric balloon placement: an analysis of 159 cases at a single center.

BACKGROUND: Intragastric balloon placement is an ideal weight loss method for those unfit or unwilling to undergo surgery. It is not known if multidisciplinary team management helps these patients the way it does with those who enroll in bariatric surgery programs. OBJECTIVES: The primary objective was to assess the efficacy of intragastric balloon on weight loss after a 6-month follow-up and the secondary objective was to assess the impact of multidisciplinary team intervention (psychological consultation, nutritional follow-up, and regular physical activity) on weight loss in the study patients. SETTING: Referral military tertiary care center, Mexico. METHODS: Retrospective study of 159 patients treated with intragastric balloon between June 2011 and December 2016 in a single institution with aims of assessing its efficacy and the impact of regular exercise, supervised diet, and psychological consultation during the intervention. RESULTS: One hundred fifty-nine patients were enrolled. There were no drop-offs nor patients lost to follow-up. The mean initial weight was 92.6 ± 12.6 kg with a decrease to a mean of 80.7 ± 12.4 kg at 6 months with a mean reduction of 11.9 kg (P < .0001). The initial mean body mass index (BMI) of the population was 33.8 ± 2.8 kg/m2, which decreased to a mean of 29.5 ± 3.3 kg/m2 of BMI. The mean BMI units lost was 4.3 kg/m2 (P < .0001). A 50.8 ± 33.8% excess weight loss was observed (P < .0001), and a mean 12.6 ± 7.6% of total weight loss was found (P < .0001). Significant interventions on BMI at 6 months were psychological consultation associated with a mean BMI reduction of 6.0 ± 3.0 kg/m2 versus a mean BMI reduction of 4.1 ± 1.9 kg/m2 of those who did not (P < .0001) and physical activity with a mean BMI reduction of 4.8 ± 2.3 kg/m2 for those who did exercise versus a mean BMI reduction of 4.1 ± 2.0 kg/m2 for those who did not (P = .041). CONCLUSIONS: Intragastric balloon managed patients get additional benefit on weight loss with psychological follow-up and exercise during the intervention. Given the retrospective nature of the study, further studies are needed to definitive conclusions.

Aislamiento e identificación de microorganismos potencialmente amilolíticos y celulolíticos de suelos de humedales de Bogotá / Isolation and identification of potential amylolytic and cellulolytic microorganisms from soil of Bogota wetlands

RESUMEN Las amilasas y celulasas de origen microbiano se han utilizado desde hace más de tres décadas en la industria. El aislamiento de microorganismos nativos con capacidad amilolítica y celulolítica es el punto de partida para aprovechar la biodiversidad microbiana en la producción de amilasas y celulasas con características específicas que permitan obtener nuevos productos y optimizar procesos industriales donde estas sean aplicables. El objetivo de este trabajo fue aislar, a partir de suelo de cinco humedales en Bogotá, cepas microbianas productoras de enzimas amilolíticas y celulolíticas. Se realizó la medición de halos de hidrólisis en agar almidón y agar carboximetilcelulosa. Se evaluó la actividad enzimática por medio de la producción de azúcares reductores, determinados mediante la técnica del ácido 3,5 dinitrosalicílico. Se seleccionaron cuatro aislamientos amilolíticos diferentes, todos identificados como Bacillus amyloliquefaciens, con actividades entre 480±35 y 752±33 U/mL a 60°C. Cinco aislamientos celulolíticos diferentes fueron seleccionado, dos identificados como Bacillus amyloliquefaciens, dos como Yersinia massiliensis y uno como Stenotrophomonas nitritireducens, con actividades enzimáticas entre 13.82 ± 2.5 y 19.11 ± 2.3 U/mL a 50°C. Estos resultados demuestran que dentro de la biodiversidad de los suelos de humedales de Bogotá existen microrganismos productores de amilasas y celulasas que podrían ser aplicadas en procesos industriales.

ABSTRACT The amylases and cellulases obtained from microorganisms have been used since more than three decades in industry. The isolation of native microbial strains with amylolytic and cellulolytic ability is the starting point to make the best of microbial biodiversity and support the production of amylases and cellulases with novel characteristics to obtain new products and optimize industrial processes where these enzymes can be applied. The objective of this work was to isolate microbial strains with the capacity to produce amylolytic and cellulolytic enzymes from the soil of five wetlands in Bogotá. Hydrolysis halos measurements in starch agar and carboxymethylcellulose agar were performed. The enzymatic activity was determined through the production of reducing sugars which were determined by 3,5-dinitrosalicylic acid method. Four different amylolytic isolations were selected and all of them were identified as Bacillus amyloliquefaciens. The amylolytic activity was between 480 ± 35 and 752±33 U/mL at 60°C. Five different cellulolytic strains were selected and two of them were identified as Bacillus amyloliquefaciens, two as Yersinia massiliensis and one as Stenotrophomonas nitritireducens. Their cellulolytic activities were from 13.82 ± 2.51 to 19.11 ± 2.3 U/mL at 50°C. These results demonstrate that as a part of the Bogota wetlands soil biodiversity there are microorganisms producing amylases and cellulases which might be applied in industrial processes.

Análisis de las lesiones encefálicas en resonancia magnética en pacientes con señales transitorias de alta intensidad detectadas por Doppler transcraneal / Analysis of Encephalic Lesions in Magnetic Resonance Imaging in patients with High Intensity Transient Signals detected on Transcranial Doppler

Resumen Objetivo: Describir los hallazgos en resonancia magnética (RM) de encéfalo en pacientes menores de 65 años que fueron estudiados por Doppler transcraneal (DTC) con contraste de microburbujas, con antecedentes de accidente cerebrovascular (ACV) criptogénico y sospecha de foramen oval permeable (FOP). Materiales y métodos: Este estudio transversal retrospectivo incluyó pacientes de ambos sexos, menores de 65 años. Resultados: Nuestra muestra (n = 47, 47% masculino y 53% femenino, edad media de 42 años) presentó señales transitorias de alta intensidad (HITS, por su sigla en inglés) positivo en el 61,7% y HITS-negativo en el 38,3%. En pacientes HITS-positivo, predominaron las lesiones a nivel de las fibras en U subcorticales, únicas o múltiples con distribución bilateralmente simétrica. En pacientes con HITS moderados, predominaron las lesiones en el territorio vascular de la circulación posterior. Conclusión: En pacientes menores de 65 años con ACV criptogénico y lesiones en fibras en U subcorticales, únicas o múltiples con distribución bilateral y simétrica, debe tenerse en cuenta un FOP como posible causa de dichas lesiones.

Abstract Objectives: To analyze the findings on brain magnetic resonance imaging (MRI) in patients less than 65 years of age with history of cryptogenic stroke and suspected patent foramen ovale (PFO) who were studied with Contrast-Transcranial Doppler. Materials and Methods: This transversal retrospective study included both, men and women less than 65 years of age. Results: Our sample (n = 47, 47% male and 53% female, average age 42 years old) had High Intensity Transient Signals (HITS)-positive in 61.7% and HITS-negative in 38.3%. In HITS-positive patients, lesions were predominantly located on the subcortical U fibers, lone or multiple bilateral symmetric distributions. In patients with moderate-severity HITS, the posterior circulation was the most affected. Conclusion: In patients less than 65 years of age with cryptogenic stroke with lesions affecting the subcortical U fibers, with unique or multiple bilateral symmetric distributions, a PFO should be considered as an underlying cause.

Maternal Metabolic Demands Caused by Pregnancy and Lactation: Association with Productivity and Offspring Phenotype in High-Yielding Dairy Ewes.

Pregnancy and lactation, especially when concurrent, create a rather metabolically demanding situation in dairy ruminants, but little is known about their effects on offspring phenotype and milk yield. Here, we evaluated the impact of pregnancy and lactation on the metabolic traits and productive performance of Lacaune dairy sheep and their offspring. Productive performance was measured in terms of milk yield, body weight (BW), body condition score (BCS), and size. Productivity was assessed during mid-pregnancy (75 ± 5 d) and late pregnancy (142 ± 4 d) and at 52 ± 5 d in the postpartum period. During pregnancy, high-yielding ewes had higher BW, BCS, plasma glucose, cholesterol, ß-OHB, and NEFA than low-yielding ewes, but lower levels of lactate and urea. High-yielding animals had lower BCS after lambing, but their lambs showed greater growth. Productivity during lactation was affected by ewe age and parity: Mature ewes (but not maiden sheep) whose BCS increased steeply during pregnancy yielded more milk in the subsequent lactation than those whose BCS did not increase. Lamb BW and size were positively associated with milk yield in the subsequent lactation. Mature ewes had higher yields than maiden sheep, and mature ewes with multiple pregnancies produced more milk than those with singleton pregnancies. Ewes with male singleton pregnancies also showed higher yield than those with female singletons. These results demonstrate that high-yielding dairy sheep, when appropriately fed and managed, can adequately cover the metabolic demands of pregnancy and high milk production (even when concurrent) without losing productivity.

Estimación de las desviaciones volumétricas de dosis entregada vs. planificada durante el tratamiento de hipertiroidismo con 131I: resultados preliminares / Estimation of volumetric dose distribution delivery deviations vs. dose planned in 131I hyperthyroidism treatment: preliminary results

Resumen En 2013, la Asociación Europea de Medicina Nuclear (AEMN) recomendó un "Procedimiento estándar" para desarrollar la dosimetría individual pretratamiento del Hipertiroidismo con 131I basada en la captación y en cinética tiroidea del paciente. Para estimar las desviaciones en la distribución de dosis entregada vs planificada al volumen tiroideo bajo este procedimiento, se desarrolló y verificó una aplicación en Matlab. Esta aplicación desarrolla el ajuste de la farmacocinética tiroidea, los cálculos de actividad acumulada, la estimación de la masa funcional tiroidea, el cálculo de la actividad a administrar para garantizar la dosis prescrita por el médico, y estimar el mapa tridimensional (3D) de dosis y los parámetros estadísticos relacionados que la caracterizan. La aplicación desarrollada se verificó empleando una imagen-maniquí y 6 farmacocinéticas conocidas. Además, se estimaron y verificaron los parámetros dosimétricos de planificación en 6 pacientes con dosis prescritas entre 150-400 Gy (promedio 241,67 Gy). Las distribuciones de actividad acumulada y de dosis absorbida fueron marcadamente heterogéneas. La distribución de dosis 3D mostró desviaciones estándar entre 18,01-27,08 % de la dosis prescrita. Las diferencias entre la dosis máxima y mínima por voxel/MBq de actividad administrada fue de 74-129 %. De acuerdo a los resultados, sólo entre el 50,2 % y 71,4 % de volumen tiroideo se tratará con la dosis prescrita ±20 %. Conclusiones: la dosis administrada al tejido tiroideo es no-homogénea y discrepa significativamente de la prescrita en algunas regiones, situación que requiere estudios posteriores más profundos con el objetivo de optimizar el tratamiento y sus resultados.

Abstract In 2013, the European Association of Nuclear Medicine Dosimetry Committee recommends a "Standard Operational Procedures for Hyperthyroidism Pre-Therapeutic Dosimetry" based on the assessment of the individual 131I uptake and kinetics. To estimate the 3D dose delivery deviations from prescribed dose during patient specific application of this SOP, a computer Matlab application was developed and verified. It was design to execute: radiopharmaceutical curve fitting, cumulated activity calculations, functional thyroid mass estimation, obtain the therapeutic planning activity to warranty the prescribed dose and produce the 3D planning dose map and related dosimetry parameters. 6 patients with 150-400Gy prescribed dose data planning (average 241,67Gy) were analysed using the developed application. The developed system was verify successfully using a test image phantom and 6 known pharmacokinetics data. The tridimensional thyroid volume cumulated activity and dose distributions were heterogeneous. 3D dose distribution showed standard deviations between 18.01-27.08 % of prescribed dose. The differences between maximum and minimum dose value per voxel/MBq were 74-129%. According to the result, between 50,2 % and 71,4 % of patient's thyroid will be treat with a dose of DP±20 % of planned dose, the rest will be overdose or sub dose. Conclusions: the 3D treatment planning dose distribution were completely no-homogenous, the significant difference observed should be study in the future more deeply in order to optimized the hyperthyroidism iodine treatment.