RESUMEN
Potent inhibitors of human cysteine proteases of the papain family have been made and assayed versus a number of relevant family members. We describe the synthesis of peptide alpha-ketoheterocyclic inhibitors that occupy binding subsites S1'-S3 of the cysteine protease substrate recognition cleft and that form a reversible covalent bond with the Cys 25 nucleophile. X-ray crystal structures of cathepsin K both unbound and complexed with inhibitors provide detailed information on protease/inhibitor interactions and suggestions for the design of tight-binding, selective molecules.
Asunto(s)
Benzoxazoles/química , Catepsinas/antagonistas & inhibidores , Catepsinas/química , Inhibidores Enzimáticos/química , Oligopéptidos/química , Animales , Ácido Aspártico/genética , Sitios de Unión/genética , Catepsina K , Catepsinas/genética , Cristalografía por Rayos X , Humanos , Cinética , Modelos Moleculares , Conejos , Proteínas Recombinantes/antagonistas & inhibidores , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Tirosina/genética , Valina/genéticaRESUMEN
Reaction alpha- and beta-xanthates 2 and 3 of sialic acid with glycosyl acceptors 5-8 in the presence phenylsulfenyl triflate (PST) as a promotor in a 2:1 mixture of CH(3)CN/CH(2)Cl(2) at low temperature affords alpha-sialosides in good yield and stereoselectivity. PST is prepared in situ by reacting benzenesulfenyl chloride with silver triflate. Less reactive acceptors 5 and 6 give a higher alpha/beta ratio than more reactive allylic alcohol 7 and primary alcohol 8; alpha-stereoselectivity is increased in a dilute solution. A possible mechanism of the reaction that involves intermediate alpha- and beta-nitrilium cations 16 and 17 is discussed.
