Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 47
Filtrar
1.
J Holist Nurs ; : 8980101231189653, 2023 Aug 07.
Artículo en Inglés | MEDLINE | ID: mdl-37545438

RESUMEN

Objectives: Normative beliefs around gender and sexuality place individuals in the Two Spirit, lesbian, gay, bisexual, trans, and queer (2SLGBTQ) community at risk for poorer health outcomes within the health care system compared with their heterosexual and cisgender counterparts, particularly within gendered areas of care including family planning and fertility intentions. The purpose of this research was to explore the effect that the normative beliefs of heteronormativity and cisnormativity had on the experiences of 2SLGBTQ people engaged in family planning, and to begin to understand how health care providers can provide appropriate, safe, and holistic care. Methods: We conducted a qualitative study using case study methodology and completing semi-structured interviews with 11 participants with diverse genders and sexualities. Findings: For members of the 2SLGBTQ community, family planning is greatly affected by ideals of normal, intersections of identities, health care systems, and community. They may face additional emotional labor and intentional decision-making when related to family planning. Heteronormativity and cisnormativity greatly impact the health care that is received. Conclusions: The findings contribute information in the limited field of research related to the 2SLGBTQ community and may support health care providers in providing holistic care.

2.
Health Promot Int ; 38(1)2023 Feb 01.
Artículo en Inglés | MEDLINE | ID: mdl-36801940

RESUMEN

Diet quality and food security are a concern in school-aged children in Canada. In 2019, the Canadian federal government announced the intention to work towards a national school food program. Understanding the factors that impact school food program acceptability can inform planning to ensure that students are willing to participate. A scoping review of school food programs in Canada completed in 2019 identified 17 peer-reviewed and 18 grey literature publications. Of these, five peer-reviewed and nine grey literature publications included a discussion of factors that impact the acceptance of school food programs. These factors were thematically analyzed into categories: stigmatization, communication, food choice and cultural considerations, administration, location and timing, and social considerations. Considering these factors while planning can help to maximize program acceptability.


Diet quality and having sufficient food to eat are concerns in school-aged children in Canada. In 2019, the Canadian federal government announced the intention to work towards a national school food program. Providing food to children in schools can only address diet concerns if children participate. Understanding the factors that impact school food program acceptability can inform planning to contribute to program acceptance. Themes of factors contributing to school food program acceptance discussed in 14 publications were identified. Themes included stigmatization, communication, food choice and cultural considerations, administration, location and timing, and social considerations. Considering these factors while planning can help to maximize school food program acceptability.


Asunto(s)
Servicios de Alimentación , Alimentos , Niño , Humanos , Canadá , Dieta , Estudiantes , Instituciones Académicas
3.
BMC Public Health ; 22(1): 210, 2022 02 01.
Artículo en Inglés | MEDLINE | ID: mdl-35100999

RESUMEN

BACKGROUND: Promoting health equity and reducing heath inequities is a foundational aim and ethical imperative in public health. There has been limited attention to and research on the ethical issues inherent in promoting health equity and reducing health inequities that public health practitioners experience in their work. The aim of the study was to explore how public health providers identified and navigated ethical issues and their management related to promoting health equity within services focused on mental health promotion and preventing harms of substance use. METHODS: Semi-structured individual interviews and focus groups were conducted with 32 public health practitioners who provided public-health oriented services related to mental health promotion and prevention of substance use harms (e.g. harm reduction) in one Canadian province. RESULTS: Participants engaged in the basic social process of navigating conflicting value systems. In this process, they came to recognize a range of ethically challenging situations related to health equity within a system that held values in conflict with health equity. The extent to which practitioners recognized, made sense of, and acted on these fundamental challenges was dependent on the degree to which they had developed a critical public health consciousness. Ethically challenging situations had impacts for practitioners, most importantly, the experiences of responding emotionally to ethical issues and the experience of living in dissonance when working to navigate ethical issues related to promoting health equity in their practice within a health system based in biomedical values. CONCLUSIONS: There is an immediate need for practice-oriented tools for recognizing ethical dilemmas and supporting ethical decision making related to health equity in public health practice in the context of mental health promotion and prevention of harms of substance use. An increased focus on understanding public health ethical issues and working collaboratively and reflexively to address the complexity of equity work has the potential to strengthen equity strategies and improve population health.


Asunto(s)
Equidad en Salud , Trastornos Relacionados con Sustancias , Canadá , Teoría Fundamentada , Promoción de la Salud , Humanos , Salud Pública , Trastornos Relacionados con Sustancias/prevención & control
5.
JMIR Res Protoc ; 10(9): e30899, 2021 09 21.
Artículo en Inglés | MEDLINE | ID: mdl-34546171

RESUMEN

BACKGROUND: Good nutrition affects children's health, well-being, and learning, and schools offer an important setting to promote healthy behaviors that can last a lifetime. Once children reach school age, they spend more of their waking hours in school than in any other environment. Children's eating habits may be easier to influence than those of adults. In Canada, households with children are more likely to experience food insecurity, and school food programs that are universally available to all children can support the development of healthy eating patterns across groups of varying socioeconomic status. There is a significant gap in the rigorous community-engaged academic research on the impact of school meal programs, especially universal ones. OBJECTIVE: The aim of this population health intervention research is to study the impact of a 2-year universal, curriculum-integrated healthy school lunch program in elementary schools in Saskatoon, Saskatchewan, Canada, on food consumption, dietary quality and food and nutrition-related knowledge, attitudes, and practices. METHODS: This population health intervention study will be conducted in 2 intervention elementary schools matched with 2 control schools. We will collect preintervention data, including objective measurements of food eaten at school and food-related knowledge, attitudes, and behaviors. This will be followed by the intervention itself, along with qualitative case studies of the intervention process in the 2 intervention schools. Then, we will collect postintervention data similar to the preintervention data. Finally, we will finish the data analysis and complete the ongoing sharing of learning from the project. RESULTS: This study was funded in April 2020 but because of the COVID-19 pandemic, data collection did not begin until May 2021. The intervention will begin in September 2021 and end in June 2023, with end point data collection occurring in May and June 2023. The case study research will begin in September 2021 and will be ongoing for the duration of the intervention. CONCLUSIONS: The opportunity we have to systematically and comprehensively study a curriculum-integrated school lunch program, as well as the promising practices for school food programs across Canada, is without precedent. INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): DERR1-10.2196/30899.

6.
BMC Public Health ; 21(1): 1567, 2021 08 18.
Artículo en Inglés | MEDLINE | ID: mdl-34407781

RESUMEN

BACKGROUND: Public health (PH) practitioners have a strong moral commitment to health equity and social justice. However, PH values often do not align with health systems values, making it challenging for PH practitioners to promote health equity. In spite of a growing range of PH ethics frameworks and theories, little is known about ethical concerns related to promotion of health equity in PH practice. The purpose of this paper is to examine the ethical concerns of PH practitioners in promoting health equity in the context of mental health promotion and prevention of harms of substance use. METHODS: As part of a broader program of public health systems and services research, we interviewed 32 PH practitioners. RESULTS: Using constant comparative analysis, we identified four systemic ethical tensions: [1] biomedical versus social determinants of health agenda; [2] systems driven agendas versus situational care; [3] stigma and discrimination versus respect for persons; and [4] trust and autonomy versus surveillance and social control. CONCLUSIONS: Naming these tensions provides insights into the daily ethical challenges of PH practitioners and an opportunity to reflect on the relevance of PH frameworks. These findings highlight the value of relational ethics as a promising approach for developing ethical frameworks for PH practice.


Asunto(s)
Equidad en Salud , Promoción de la Salud , Humanos , Principios Morales , Salud Pública , Justicia Social
7.
Can Bull Med Hist ; 38(1): 93-127, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33836136

RESUMEN

After the Second World War, health prevention work in Canada shifted from a focus on sanitation and hygiene to illness prevention and health promotion. Canada became a significant global leader, beginning with the Lalonde Report of 1974. Yet less is known about the provincial public health associations and how their work differed from that of the national body. The purpose of this article is to examine the Saskatchewan Public Health Association's (SPHA) policy work from 1954 to 1986. Utilizing meeting minutes and newsletters, we found that while both national and provincial associations made efforts to prevent accidents, reduce tobacco use, and fluoridate water, the SPHA tended to advocate more for child health, and the cautious use of nuclear power. At the same time, the SPHA's resolutions tended to ignore emerging factors shaping health, including the social determinants of health, regional inequities, lack of public trust in experts, misinformation, and human psychology. Examining the SPHA's records revealed that region mattered in preventative policy work.


Asunto(s)
Política de Salud/historia , Servicios Preventivos de Salud/historia , Salud Pública/historia , Sociedades Médicas/historia , Historia del Siglo XX , Saskatchewan
8.
Can J Diet Pract Res ; 81(4): 179-185, 2020 12 01.
Artículo en Inglés | MEDLINE | ID: mdl-32495636

RESUMEN

Purpose: Consuming nutritious food is essential to learning. The purpose of this research was to determine the diet quality of elementary school lunches, both those in meal programs and those bringing food from home, in urban and rural locations in Saskatchewan.Methods: Using a School Food Checklist and digital photography we compared food group servings and diet quality in 3 school types: urban schools with a meal program and urban and rural schools without a meal program. The total sample was 773 students.Results: Only 55% of students brought the minimum number of servings for grain products and meat and alternatives, with fewer bringing the minimum for vegetables and fruit (25.6%-34.9%), whole grains (24.1%), and milk and alternatives (14.1%). Students bringing food from home had significantly more calories in their lunches from minimally nutritious foods. Students in meal programs had the highest diet quality scores using the Healthy Eating Index adapted for school hours.Conclusions: The diet quality of elementary students' lunches needs improvement, although students in meal programs have healthier diets. Interventions targeting what children eat at school should focus on increasing the number of students meeting recommendations for healthy foods while decreasing minimally nutritious foods brought to school.


Asunto(s)
Dieta Saludable , Servicios de Alimentación , Instituciones Académicas , Niño , Dieta , Ingestión de Energía , Humanos , Almuerzo , Saskatchewan
12.
BMC Nephrol ; 19(1): 226, 2018 09 12.
Artículo en Inglés | MEDLINE | ID: mdl-30208850

RESUMEN

BACKGROUND: People with end stage renal disease (ESRD) face important health-related decisions concerning end-of-life care and the use of life-support technologies. While people often want to be involved in making decisions about their health, there are many challenges. People with advanced illness may have limited or wavering ability to participate fully in decision-making conversations - or lack decisional capacity for making decisions. Additionally, they may have a limited understanding of CPR and tend to receive inconsistent information on the process and outcome of CPR. Unfortunately, these discussions are often avoided. Shared decision-making approaches are an approach to overcoming these challenges. The objectives of this research was to design, test, and analyze a novel CPR video decision aid (VDA) with nephrology patients and their families in a clinical setting. METHODS: The Interprofessional Shared Decision-making Model was used as a framework to guide the research. A prospective quasi-experimental design included pre/posttest measures of knowledge and confidence in decision-making, and posttest only measure of uncertainty about the decision. RESULTS: Participant knowledge about CPR increased from a mean score of 4.8/9 (standard deviation [SD] = 1.65) before viewing the video to 7.5/9 (SD = 1.40) (p = 0.000) after viewing the video. Decisional self-efficacy improved slightly from 84% pre intervention (SD 17.04, range 20-100) to 86% after the intervention (SD 14.13, range 39-100) (p = 0.005) for patient participants. Before the intervention, most patients (43/49; 86%) had an order to have CPR in the physician orders and very few (7/49; 14%) had an order not to have CPR. Immediately after viewing the CPR-VDA and completing the values clarification worksheet, fewer 28/49 (57%) chose to have CPR, 13 (27%) chose not to have CPR and 8 (16%) were unsure. CONCLUSIONS: The CPR-VDA was feasible and acceptable to patients with ESRD, their families and the healthcare team. The CPR-VDA positively affected decision-making: improving patient and family knowledge about CPR, clarity of values, patients' decisional self-efficacy, the congruence between documented physician's orders and patient choice, quality of communication about CPR, while reducing decisional conflict (uncertainty) amongst patients, families, and physicians.


Asunto(s)
Reanimación Cardiopulmonar/métodos , Toma de Decisiones Clínicas/métodos , Técnicas de Apoyo para la Decisión , Fallo Renal Crónico/terapia , Participación del Paciente/métodos , Grabación en Video/métodos , Anciano , Anciano de 80 o más Años , Reanimación Cardiopulmonar/psicología , Reanimación Cardiopulmonar/normas , Estudios de Factibilidad , Femenino , Humanos , Fallo Renal Crónico/psicología , Masculino , Persona de Mediana Edad , Educación del Paciente como Asunto/métodos , Educación del Paciente como Asunto/normas , Participación del Paciente/psicología , Estudios Prospectivos , Grabación en Video/normas
13.
Int J Equity Health ; 17(1): 48, 2018 04 23.
Artículo en Inglés | MEDLINE | ID: mdl-29688855

RESUMEN

BACKGROUND: Promoting health equity within health systems is a priority and challenge worldwide. Health equity tools have been identified as one strategy for integrating health equity considerations into health systems. Although there has been a proliferation of health equity tools, there has been limited attention to evaluating these tools for their practicality and thus their likelihood for uptake. METHODS: Within the context of a large program of research, the Equity Lens in Public Health (ELPH), we conducted a concept mapping study to identify key elements and themes related to public health leaders and practitioners' views about what makes a health equity tool practical and useful. Concept mapping is a participatory mixed-method approach to generating ideas and concepts to address a common concern. Participants brainstormed responses to the prompt "To be useful, a health equity tool should…" After participants sorted responses into groups based on similarity and rated them for importance and feasibility, the statements were analyzed using multidimensional scaling, then grouped using cluster analysis. Pattern matching graphs were constructed to illustrate the relationship between the importance and feasibility of statements, and go-zone maps were created to guide subsequent action. RESULTS: The process resulted in 67 unique statements that were grouped into six clusters: 1) Evaluation for Improvement; 2) User Friendliness; 3) Explicit Theoretical Background; 4) Templates and Tools 5) Equity Competencies; and 6) Nothing about Me without Me- Client Engaged. The result was a set of concepts and themes describing participants' views of the practicality and usefulness of health equity tools. CONCLUSIONS: These thematic clusters highlight the importance of user friendliness and having user guides, templates and resources to enhance use of equity tools. Furthermore, participants' indicated that practicality was not enough for a tool to be useful. In addition to practical characteristics of the tool, a useful tool is one that encourages and supports the development of practitioner competencies to engage in equity work including critical reflections on power and institutional culture as well as strategies for the involvement of community members impacted by health inequities in program planning and delivery. The results of this study will be used to inform the development of practical criteria to assess health equity tools for application in public health.


Asunto(s)
Equidad en Salud/organización & administración , Promoción de la Salud/métodos , Desarrollo de Programa/métodos , Administración en Salud Pública/métodos , Salud Pública/estadística & datos numéricos , Análisis por Conglomerados , Humanos , Análisis Multivariante
14.
Health Promot Chronic Dis Prev Can ; 38(1): 29-35, 2018 Jan.
Artículo en Inglés, Francés | MEDLINE | ID: mdl-29323865

RESUMEN

INTRODUCTION: Many North American cities have a built environment that provides access to energy-dense food and little opportunity for active living. Urban agriculture contributes to a positive environment involving food plant cultivation that includes processing, storing, distributing and composting. It is a means to increase local food production and thereby improve community health. The purpose of this study was to understand how participating in urban agriculture can help to empower young adults and build capacity for growing food in the city. METHODS: This was a qualitative study of seven participants (five Indigenous and two non-Indigenous) between the ages of 19 and 29 years, engaged as interns in an urban agriculture project known as "askîy" in Saskatoon, Saskatchewan, Canada in 2015. We used a case-study design and qualitative analysis to describe the participants' experience based on the sustainable livelihoods framework. RESULTS: A collaborative approach had a great effect on the interns' experiences, notably the connections formed as they planned, planted, tended, harvested and sold the produce. Some of the interns changed their grocery shopping habits and began purchasing more vegetables and questioning where and how the vegetables were produced. All interns were eager to continue gardening next season, and some were planning to take their knowledge and skills back to their home reserves. CONCLUSION: Urban agriculture programs build capacity by providing skills beyond growing food. Such programs can increase local food production and improve food literacy skills, social relationships, physical activity and pride in community settings.


INTRODUCTION: De nombreuses villes d'Amérique du Nord ont construit un environnement donnant accès à des aliments riches en calories et laissant peu de possibilités de mener une vie active. L'agriculture urbaine contribue à un environnement favorable grâce à la culture de végétaux destinés à l'alimentation, ce qui implique leur transformation, leur entreposage, leur distribution et leur compostage. Elle constitue un moyen d'augmenter la production alimentaire locale et d'améliorer ainsi la santé dans les collectivités. Cette étude avait comme objectif de comprendre comment la participation à l'agriculture urbaine peut contribuer à l'autonomisation de jeunes adultes et au renforcement des compétences à cultiver des aliments en ville. MÉTHODOLOGIE: Il s'agissait d'une étude qualitative reposant sur sept participants (cinq Autochtones et deux non-Autochtones) de 19 à 29 ans, recrutés comme stagiaires dans un projet d'agriculture urbaine appelé « Askîy ¼ à Saskatoon (Saskatchewan, Canada) en 2015. Nous avons utilisé un modèle d'étude de cas et une analyse qualitative pour décrire l'expérience des participants en fonction du Cadre des moyens de subsistance durables. RÉSULTATS: L'approche collaborative a eu un effet majeur sur l'expérience des stagiaires, en particulier dans les liens qu'ils ont formés au fil de la planification, de la plantation, de l'entretien, de la récolte et de la vente des produits. Certains stagiaires ont changé leurs habitudes en matière d'épicerie et ont commencé à acheter plus de légumes et à demander où et comment sont produits les légumes. Tous les stagiaires étaient enthousiastes à l'idée de jardiner de nouveau l'année suivante, et certains d'entre eux planifiaient d'appliquer leurs connaissances et leurs compétences dans leur réserve d'origine. CONCLUSION: Les programmes d'agriculture urbaine renforcent les compétences en permettant d'acquérir des habiletés qui vont au-delà de la culture d'aliments. Ils augmentent la production alimentaire locale et améliorent les compétences en littératie alimentaire, les relations sociales, l'activité physique et la fierté au sein de la collectivité.


Asunto(s)
Agricultura , Ciudades , Dieta Saludable , Grupos de Población , Salud Urbana/normas , Adulto , Agricultura/métodos , Agricultura/organización & administración , Canadá/epidemiología , Dieta Saludable/etnología , Dieta Saludable/métodos , Dieta Saludable/psicología , Educación , Femenino , Industria de Alimentos/métodos , Conductas Relacionadas con la Salud , Promoción de la Salud/métodos , Humanos , Masculino , Plantas , Grupos de Población/psicología , Grupos de Población/estadística & datos numéricos , Salud Pública/métodos , Salud Pública/normas , Investigación Cualitativa , Mejoramiento de la Calidad
15.
BMC Public Health ; 17(1): 803, 2017 10 11.
Artículo en Inglés | MEDLINE | ID: mdl-29020953

RESUMEN

BACKGROUND: Strengthening public health systems has been a concern in Canada in the wake of public health emergencies. In one Canadian province, British Columbia, a high priority has been placed on the role of evidence to guide decision making; however, there are numerous challenges to using evidence in practice. The National Collaborating Centre for Methods and Tools therefore developed the Evidence Informed Public Health Framework (EIPH), a seven step guide to assist public health practitioners to use evidence in practice. We used this framework to examine the evidence literacy of public health practitioners in BC. METHODS: We conducted a secondary analysis of two separate qualitative studies on the public health renewal process in which the use and understanding of evidence were key interview questions. Using constant comparative analysis, we analyzed the evidence-related data, mapping it to the categories of the EIPH framework. RESULTS: Participants require both data and evidence for multiple purposes in their daily work; data may be more important to them than research evidence. They are keen to provide evidence-based programs in which research evidence is balanced with community knowledge and local data. Practitioners recognise appraisal as an important step in using evidence, but the type of evidence most often used in daily practice does not easily lend itself to established methods for appraising research evidence. In the synthesis stage of the EIPH process, synthesized evidence in the form of systematic reviews and practice guidelines is emphasized. Participants, however, need to synthesize across the multiple forms of evidence they use and see the need for more skill and resources to help them develop skill in this type of synthesis. CONCLUSIONS: Public health practitioners demonstrated a good level of evidence literacy, particularly at the collective level in the organization. The EIPH framework provides helpful guidance in how to use research evidence in practice, but it lacks support on appraising and synthesizing across the various types of evidence that practitioners consider essential in their practice. We can better support practitioners by appreciating the range of evidence they use and value and by creating tools that help them to do this.


Asunto(s)
Práctica Clínica Basada en la Evidencia , Alfabetización en Salud , Práctica de Salud Pública , Colombia Británica , Humanos , Investigación Cualitativa
16.
AIMS Public Health ; 3(1): 94-109, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-29546149

RESUMEN

Public health systems have suffered infrastructure losses worldwide. Strengthening public health systems requires not only good policies and programs, but also development of new research methodologies to support public health systems renewal. Our research team considers public health systems to be complex adaptive systems and as such new methods are necessary to generate knowledge about the process of implementing public health programs and services. Within our program of research, we have employed situational analysis as a method for studying complex adaptive systems in four distinct research studies on public health program implementation. The purpose of this paper is to demonstrate the use of situational analysis as a method for studying complex systems and highlight the need for further methodological development.

17.
Can J Diet Pract Res ; 76(3): 140-5, 2015 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-26280794

RESUMEN

The term food literacy is emergent, and as a result the literature reflects a great variety of definitions. Simultaneously, new research and food literacy programming is being developed without an agreed upon definition of what food literacy is and how food skills, food security, and health literacy may fit with the definition. We undertook a scoping review and conceptual analysis to identify how the term is understood and to determine shared components of definitions. We found that although most definitions included a nutrition and food skills component, there was great variation in how the ability to access, process, and enjoy food was affected by our complex food system. We propose a definition of food literacy that includes the positive relationship built through social, cultural, and environmental experiences with food enabling people to make decisions that support health. We offer a framework that situates food literacy at the intersection between community food security and food skills, and we assert that behaviours and skills cannot be separated from their environmental or social context. The proposed definition and framework are intended to be guiding templates for academics and practitioners to position their work in education and advocacy, bringing together separate spheres for collective action.


Asunto(s)
Dieta , Alimentos , Alfabetización en Salud , Canadá/epidemiología , Servicios de Salud Comunitaria , Servicios de Alimentación , Abastecimiento de Alimentos , Conocimientos, Actitudes y Práctica en Salud , Promoción de la Salud , Estado de Salud , Humanos , Política Nutricional , Ciencias de la Nutrición/educación , Obesidad/epidemiología
18.
J Surg Res ; 195(2): 588-95, 2015 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-25772147

RESUMEN

BACKGROUND: Endoplasmic reticulum (ER) stress and autophagy each play important roles in hepatocyte cell injury. We hypothesized that gene expression of C/EBP-homologous protein (CHOP) and the BH3 proteins Bcl2-interacting mediator of cell death (BIM) and BH3-interacting domain death agonist (BID) are involved in a complex interplay that regulates ER stress-induced autophagy and cell death. MATERIALS AND METHODS: Hepatocytes were cultured from lean Zucker rats. Confluent hepatocytes were incubated with single or combined small interfering RNA for CHOP, BIM, and/or BID for 24 h providing gene inhibition. Incubation with tunicamycin (TM) for another 24 h stimulated ER stress. Quantitative real-time polymerase chain reaction determined the expression levels of CHOP, BIM, and BID. Immunostaining with microtubule-associated protein 1 light chain 3 measured autophagy activity. Trypan blue exclusion determined the cell viability. RESULTS: TM treatment increased the messenger RNA levels of CHOP and BIM but decreased the messenger RNA levels of BID. TM increased autophagy and decreased cell viability. Individual inhibition of CHOP, BIM, or BID protected against autophagy and cell death. However, simultaneous treatment with any combination of CHOP, BIM, and BID small interfering RNAs reduced autophagy activity but increased cell death independent of ER stress induction. CONCLUSIONS: Autophagy in hepatocytes results from acute ER stress and involves interplay, at the gene expression level, of CHOP, BIM, and BID. Inhibition of any one of these individual genes during acute ER stress is protective against cell death. Conversely, inhibition of any two of the three genes results in increased nonautophagic cell death independent of ER stress induction. This study suggests interplay between CHOP, BIM, and BID expression that can be leveraged for protection against ER stress-related cell death. However, disruption of the CHOP/BH3 gene expression homeostasis is detrimental to cell survival independent of other cellular stress.


Asunto(s)
Proteínas Reguladoras de la Apoptosis/fisiología , Autofagia/fisiología , Proteína Proapoptótica que Interacciona Mediante Dominios BH3/fisiología , Hepatocitos/fisiología , Proteínas de la Membrana/fisiología , Proteínas Proto-Oncogénicas/fisiología , Factor de Transcripción CHOP/fisiología , Animales , Proteínas Reguladoras de la Apoptosis/genética , Proteína Proapoptótica que Interacciona Mediante Dominios BH3/genética , Proteína 11 Similar a Bcl2 , Células Cultivadas , Estrés del Retículo Endoplásmico , Regulación de la Expresión Génica , Masculino , Proteínas de la Membrana/genética , Proteínas Proto-Oncogénicas/genética , ARN Interferente Pequeño/genética , Ratas , Ratas Zucker , Factor de Transcripción CHOP/genética
19.
Wound Repair Regen ; 22(4): 515-20, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24898050

RESUMEN

Recurrent injury has been implicated in the development of chronic diabetic wounds. We have developed a chronic diabetic wound model based upon recurrent injury in diabetic mice. We hypothesized that dysregulation of collagen production at both the mRNA and microRNA levels contributes to the development of chronic diabetic wounds. To test this, both diabetic and nondiabetic mice were made to undergo recurrent injury. Real-time PCR for TGF-ß1, SMAD-3, Col1α1, Col3α1, microRNA-25, and microRNA-29a and Western blot for collagen I and III were performed 7 days following each injury. Diabetic wounds displayed decreased collagen at all time points. This was associated with dysregulated collagen production at both the gene and microRNA levels at all time points. Following the final injury, however, diabetic collagen production significantly improved. This appeared to be due to a substantial decrease in both microRNAs as well as an increase in the expression of collagen pathway genes. That dysregulated collagen production progressed throughout the course of wounding suggests that this is one factor contributing to the development of chronic diabetic wounds. Future studies using this model will allow for the determination of other factors that may also contribute to the development and/or persistence of chronic diabetic wounds.


Asunto(s)
Colágeno/metabolismo , Complicaciones de la Diabetes/metabolismo , Úlcera Cutánea/metabolismo , Piel/lesiones , Piel/metabolismo , Cicatrización de Heridas , Animales , Fenómenos Biomecánicos , Western Blotting , Enfermedad Crónica , Colágeno/genética , Colágeno Tipo I/metabolismo , Colágeno Tipo III/metabolismo , Diabetes Mellitus Experimental/metabolismo , Elasticidad , Ratones , Ratones Endogámicos NOD , MicroARNs/metabolismo , ARN Mensajero/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Úlcera Cutánea/etiología , Proteína smad3/metabolismo , Factor de Crecimiento Transformador beta1/metabolismo
20.
Wound Repair Regen ; 22(3): 406-14, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24844340

RESUMEN

Wound size impacts the threshold between scarless regeneration and reparative healing in the fetus with increased inflammation showed in fetal scar formation. We hypothesized that increased fetal wound size increases pro-inflammatory and fibrotic genes with resultant inflammation and fibroplasia and that transition to scar formation could be reversed by overexpression of interleukin-10 (IL-10). To test this hypothesis, 2-mm and 8-mm dermal wounds were created in mid-gestation fetal sheep. A subset of 8-mm wounds were injected with a lentiviral vector containing the IL-10 transgene (n = 4) or vehicle (n = 4). Wounds were harvested at 3 or 30 days for histology, immunohistochemistry, analysis of gene expression by microarray, and validation with real-time polymerase chain reaction. In contrast to the scarless 2-mm wounds, 8-mm wounds showed scar formation with a differential gene expression profile, increased inflammatory cytokines, decreased CD45+ cells, and subsequent inflammation. Lentiviral-mediated overexpression of the IL-10 gene resulted in conversion to a regenerative phenotype with decreased inflammatory cytokines and regeneration of dermal architecture. In conclusion, increased fetal wounds size leads to a unique gene expression profile that promotes inflammation and leads to scar formation and furthermore, these results show the significance of attenuated inflammation and IL-10 in the transition from fibroplasia to fetal regenerative healing.


Asunto(s)
Cicatriz/patología , Inflamación/patología , Interleucina-10/metabolismo , Piel/patología , Cicatrización de Heridas , Heridas y Lesiones/patología , Animales , Cicatriz/embriología , Femenino , Feto , Fibroblastos , Expresión Génica , Inmunohistoquímica , Inflamación/embriología , Fenotipo , Embarazo , Regeneración , Ovinos , Piel/embriología , Heridas y Lesiones/embriología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...