5'/3' RACE method for sequencing the 5' and 3' untranslated regions of Zika virus.

The spread of Zika virus (ZIKV) from the African continent to the Americas promoted its molecular evolution, as reflected by mutations in its RNA genome. Most of the ZIKV genome sequences in the GenBank database have incomplete 5' and 3' UTR sequences, reflecting the deficiency of whole-genome sequencing technologies to resolve the sequences of the genome ends. We modified a protocol for rapid amplification of cDNA ends (RACE) to determine the complete sequences of the 5' and 3' UTRs of a previously reported ZIKV isolate (GenBank no. MH544701.1). This strategy is useful for determining 5' and 3' UTR sequences of ZIKV isolates and will be useful for comparative genomics applications.

Striving for Equity in Community Mental Health: Opportunities and Challenges for Integrating Care for BIPOC Youth.

Supporting the mental health of youth who identify as Black, Indigenous, or Persons of Color (BIPOC) continues to be a challenge for clinicians and policymakers alike. Children and adolescents are a vulnerable population, and for BIPOC youth, these vulnerabilities are magnified by the effects of structural, interpersonal, and internalized racism. Integration of psychiatric care into other medical settings has emerged as an evidence-based method to improve access to psychiatric care, but to bridge the gap experienced by BIPOC youth, care must extend beyond medical settings to other child-focused sectors, including local governments, education, child welfare, juvenile legal systems, and beyond. Intentional policy decisions are needed to incentivize and support these systems, which typically rely on coordination and collaboration between clinicians and other stakeholders. Clinicians must be trauma-informed and strive for structural competency to successfully navigate and advocate for collaborative systems that benefit BIPOC youth.

La crítica de la psicología, trabajo y la pandemia de Covid-19* / The criticism of psychology, work, and the Covid-19 pandemic

Ante la pandemia de Covid-19 causada por el vírus SARS-CoV-2 que puso a los trabajadores de la región lationamericana en una condición de mayor vulnerabildad, en este artículo se consideran algunas de las respuestas proporcionadas por la psicología convencional de corte industrial-organizacional, las cuales han estado caracterizadas por su enfasis técnico y acrítico dirigido a responsabilizar a los trabajadores de la actual situación. Tambien son analizadas las propuestas construidas por la psicología crítica descritas como soluciones colectivas que convocan instancias Estatales, organizacionales, sindicales y gremiales para diseñar intervencinoes que contribuyan a mitigar la situacion de mayor vulnerabilidad laboral. Por último, son consideradas las respuestas derivadas de perspectivas externas a la psicologia que examinan criticamente esta disciplina para mostrar las operaciones de gobierno que son posibles gracias a las intervenciones psicológicas referidas a la contencion de la pandemia. Se señala que la llamada crisis de la crítica estaría permeándose tanto en aquellas psicologías que asumen perspectivas no hegemónicas, como en la crítica misma de la psicología cuando consideran la situación actual.(AU)

Diante da pandemia da Covid-19 causada pelo vírus SARS-CoV-2, que colocou os trabalhadores da região latino-americana em uma condição mais vulnerável, neste artigo se considera algumas das respostas fornecidas pela psicologia convencional, que tem se caracterizado por sua ênfase técnica e acrítica no sentido de responsabilizar os trabalhadores pela situação atual. Também se analisam as propostas construídas pela psicologia crítica descritas como soluções coletivas que apelam às instâncias estatais, organizacionais, sindicais e grupais para conceber intervenções que ajudem a mitigar a situação de maior vulnerabilidade no trabalho. Por fim, são consideradas as respostas derivadas de perspectivas externas à psicologia que examinam criticamente essa disciplina para mostrar as operações de governo que são possíveis por meio de intervenções psicológicas relacionadas à contenção da pandemia. Ressalta-se que a chamada crise da crítica estaria permeando tanto aquelas psicologias que assumem perspectivas não hegemônicas, como a própria crítica da psicologia em si ao se considerar a situação atual.(AU)

Faced with the Covid-19 pandemic caused by the SARS-CoV-2 virus that put workers in the Latin American region in a more vulnerable condition, this article considers some of the responses provided by conventional industrial-organizational psychology, which have been characterized by their technical and uncritical emphasis aiming at holding workers accountable for the current situation. Also, the proposals constructed by critical psychology are analyzed to indicate that these collective solutions appeal to State, organizational, union and group instances to design interventions that contribute to mitigate the situation of greater vulnerability at work. Finally, the responses derived from perspectives external to psychology that critically examine this discipline are considered to show the government operations that are possible through psychological interventions related to the containment of the pandemic. It is pointed out how the so-called crisis of criticism is permeating those psychologies that assume non-hegemonic perspectives, as well as in the criticism of psychology itself when they consider the current situation.(AU)

Determination of the Ratio of the Decantation Time and the Separation of Components in Lipoaspirate.

Decantation of the lipoaspirate is one of the most common techniques used to prepare the fat graft. The aim of the study was to determine the ideal time of decantation that provides the best separation of the components without compromising the viability of the adipocytes. METHODS: Thirty milliliters of fat were obtained from 11 healthy adults and decanted at room temperature for 0, 30, and 60 minutes. After decantation, the infiltration liquid and the remnant fat were measured with a volumetric pipette. Once the solution was removed, the remnant fat was centrifuged at 3000 rpm for 5 minutes to separate any residual solution, to measure the amount of actual fat obtained at that time point. Viability was determined with trypan blue staining for all the samples. RESULTS: After decantation, 9.4 ± 0.79 mL of fat was obtained at time 0, whereas 7.7 ± 1.56 mL was obtained at 30 minutes and 6.9 ± 0.92 mL at 60 minutes. Actual fat volume was 6.6 ± 1.56 mL, 5.5 ± 1.39, and 5.26 ± 1.3 mL, respectively. Viability at time 0 was 73.33 ± 0.06%, 72.57 ± 0.1% at 30 minutes, and 59.3 ± 0.09% at 60 minutes (P = 0.004). RESULTS: The fat grafting, processed by decantation, will have the best performance within a period of 30 minutes after harvesting, where the best rate of viability and separation of components will be achieved.

Effect of refrigeration in the viability and apoptosis of decanted adipocytes after liposuction.

OBJETIVO: Evaluar el efecto de la refrigeración en la apoptosis y la viabilidad del lipoaspirado en las primeras 2 horas después de la toma. MÉTODO: Se incluyeron 20 pacientes que fueron sometidas a una liposucción del abdomen por razones estéticas. Se obtuvieron 5 ml de grasa y se procesaron para su estudio. La viabilidad se determinó usando azul tripano. La apoptosis se determinó usando el ensayo TUNEL. RESULTADOS: Todas las pacientes eran mujeres, con una edad media de 36.5 años (rango: 21-67). Con respecto a la viabilidad, en el tiempo 0, en el grupo control fue del 59.08 ± 24% y en el grupo de refrigeración fue del 60.96 ± 22%; a los 60 minutos, los valores fueron del 50.82 ± 21% y el 55 ± 32.6%, respectivamente (p = 0.74); y a los 120 minutos, fueron del 42.69 ± 20.85% y el 50.33 ± 21%, respectivamente. En cuanto a la apoptosis, el porcentaje de células apoptóticas en el tiempo 0 fue del 37.87 ± 9.7% para el grupo de control y del 34.28 ± 9.74% para las muestras refrigeradas; a los 60 minutos, del 51.11 ± 8.64% y el 45.94 ± 9.15%, respectivamente; y a los 120 minutos, del 62.97 ± 13.33% y el 55.81 ± 9.45%, respectivamente. CONCLUSIONES: Refrigerar el lipoaspirado a 4 °C disminuyó la mortalidad y la apoptosis de los adipocitos en menos del 10% en las primeras 2 horas desde la toma.

OBJECTIVE: To evaluate the effect of refrigeration in the apoptosis and viability of the lipoaspirate in the first 2 h after harvesting. METHODS: 20 consecutive patients who underwent liposuction from the abdomen for esthetic reasons were included. 5 ml of fat were obtained and processed for study. The viability was obtained using trypan blue. Apoptosis was determined using TUNEL assay. RESULTS: All patients were female with a median age of 36.5 (21-67) years. On regard of the viability, at time 0, the viability in the control group was 59.08 ± 24% and 60.96 ± 22% in the refrigeration group. At 60 min, the values were 50.82 ± 21% versus 55 ± 32.6% (p = 0.74) and a 120 min, 42.69 ± 20.85% and 50.33 ± 21% respectively. On regard of apoptosis, the percentage of apoptotic cells at time 0 was 37.87 ± 9.7% for the control group and 34.28 ± 9.74% for refrigerated samples. At 60 min 51.11 ± 8.64% versus 45.94 ± 9.15% and at 120 min, 62.97 ± 13.33% versus 55.81 ± 9.45% respectively. CONCLUSIONS: Refrigerating the lipoaspirate at 4 °C decreased the mortality and apoptosis of the adipocytes in <10% within the first 2 h from harvesting.

A Test for the Clinical Evaluation of the Flexor Digitorum Superficialis of the Fifth Finger.

Physical examination is essential in diagnosing tendinous lesions. This is particularly true of the flexor digitorum superficialis of the little finger (FDS5), which is functionally absent in approximately 30% of the population. The objective of our study was to determine the diagnostic value of 3 clinical tests commonly used to assess the function of this tendon. METHODS: Patients with wounds of the FDS5 were included in this study. Under local or regional anesthesia, 3 described clinical tests were performed to assess the function of the FDS5: (i) the classic test; (ii) Stein's modified test, and (iii) Mecott's modified test. We determined sensitivity, specificity, and predictive values of all such tests. The integrity of the tendon was assessed surgically. Correlation among blinded observers was also established. RESULTS: A total of 28 subjects with a mean age of 28 years (ranging from 5 to 56) participated in this study. The classic test obtained a sensitivity of 100% and a specificity of 72%; Stein's test resulted in a sensitivity of 83% and a specificity of 95%, whereas Mecott's test reached a sensitivity of 100% and a specificity of 95%. CONCLUSIONS: Among the 3 tests described and used in our study, Mecott's modified test proved to be more sensitive and specific than the other two; therefore, we consider this to be the test that should be used in determining the integrity of the FDS5.

CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells.

Many secretory cells increase the synthesis and secretion of cargo proteins in response to specific stimuli. How cells couple increased cargo load with a coordinate rise in secretory capacity to ensure efficient transport is not well understood. We used thyroid cells stimulated with thyrotropin (TSH) to demonstrate a coordinate increase in the production of thyroid-specific cargo proteins and ER-Golgi transport factors, and a parallel expansion of the Golgi complex. TSH also increased expression of the CREB3L1 transcription factor, which alone caused amplified transport factor levels and Golgi enlargement. Furthermore, CREB3L1 potentiated the TSH-induced increase in Golgi volume. A dominant-negative CREB3L1 construct hampered the ability of TSH to induce Golgi expansion, implying that this transcription factor contributes to Golgi expansion. Our findings support a model in which CREB3L1 acts as a downstream effector of TSH to regulate the expression of cargo proteins, and simultaneously increases the synthesis of transport factors and the expansion of the Golgi to synchronize the rise in cargo load with the amplified capacity of the secretory pathway.

De la relación entre la Psicología y el trabajo a la luz de sus dimensiones coloniales / On the relationship between Psychology and work in the light of its colonial dimensions

Resumen Las herramientas propuestas en los marcos poscoloniales y decoloniales no han sido de mayor interés para los investigadores que se ocupan de la interrelación entre el conocimiento psicológico y el mundo laboral. Entonces, los estudios críticos han dejado de lado las implicaciones que tiene este conocimiento cuando "viaja" más allá de las fronteras de los países en donde se produce la Psicología. En este artículo se examinan algunas "dimensiones coloniales" que emergen de las articulaciones del "complejo psi" cuando se vincula con el mundo del trabajo en lugares en donde no se producen los objetos psicológicos, sino en donde estos son apropiados, replicados o hibridados para su uso cotidiano en los ámbitos laborales.

Abstract The tools proposed in the postcolonial and de-colonial frameworks have not been of interest to researchers preoccupied with the interrelation between the psychological knowledge and the world of work. Thus, critical studies have left aside the implications that this knowledge has when it goes beyond the borders in which Psychology is produced. This article examines some "colonial dimensions" that emerged from the articulations of the "psy complex" in places that do not produce psychological objects, but which are appropriate, replicated and hybridized for being used in workplaces.

Preservation of epithelial cell barrier function and muted inflammation in resistance to allergic rhinoconjunctivitis from house dust mite challenge.

BACKGROUND: An emerging paradigm holds that resistance to the development of allergic diseases, including allergic rhinoconjunctivitis, relates to an intact epithelial/epidermal barrier during early childhood. Conceivably, the immunologic and genomic footprint of this resistance is preserved in nonatopic, nonallergic adults and is unmasked during exposure to an aeroallergen. OBJECTIVE: The aim of this study was to obtain direct support of the epithelial/epidermal barrier model for allergic rhinoconjunctivitis. METHODS: Twenty-three adults allergic to house dust mites (HDMs) (M+) and 15 nonsensitive, nonallergic (M-) participants completed 3-hour exposures to aerosolized HDM (Dermatophagoides pteronyssinus) powder on 4 consecutive days in an allergen challenge chamber. We analyzed: (1) peripheral blood leukocyte levels and immune responses; and (2) RNA sequencing-derived expression profiles of nasal cells, before and after HDM exposure. RESULTS: On HDM challenge: (1) only M+ persons developed allergic rhinoconjunctivitis symptoms; and (2) peripheral blood leukocyte levels/responses and gene expression patterns in nasal cells were largely concordant between M+ and M- participants; gross differences in these parameters were not observed at baseline (pre-exposure). Two key differences were observed. First, peripheral blood CD4+ and CD8+ T-cell activation levels initially decreased in M- participants versus increased in M+ participants. Second, in M- compared with M+ participants, genes that promoted epidermal/epithelial barrier function (eg, filament-aggregating protein [filaggrin]) versus inflammation (eg, chemokines) and innate immunity (interferon) were upregulated versus muted, respectively. CONCLUSION: An imprint of resistance to HDM challenge in nonatopic, nonallergic adults was muted T-cell activation in the peripheral blood and inflammatory response in the nasal compartment, coupled with upregulation of genes that promote epidermal/epithelial cell barrier function.

Spatial-Temporal Study of Rab1b Dynamics and Function at the ER-Golgi Interface.

The GTPase Rab1b is involved in ER to Golgi transport, with multiple Rab1b effectors (located at ERES, VTCs and the Golgi complex) being required for its function. In this study, we performed live-cell dual-expression studies to analyze the dynamics of Rab1b and some effectors located at the ERES-Golgi interface. Rab1b occupied widely distributed mobile punctate and tubular structures, displaying a transient overlaps with its effectors and showing that these overlaps occurred at the same time in spatially distinct steps of ER to Golgi transport. In addition, we assessed Rab1b dynamics during cargo sorting by analyzing the concentration at ERES of a Golgi protein (SialT2-CFP) during Brefeldin A washout (BFA WO). Rab1b was associated to most of the ERES structures, but at different times during BFA WO, and recurrently SialT2-CFP was sorted in the ERES-Rab1b positive structures. Furthermore, we reveal for first time that Rab1b localization time at ERES depended on GBF1, a Rab1b effector that acts as the guanine nucleotide exchange factor of Arf1, and that Rab1b membrane association/dissociation dynamics at ERES was dependent on the GBF1 membrane association and activity, which strongly suggests that GBF1 activity modulates Rab1b membrane cycling dynamic.

Lateral diffusion, function, and expression of the slow channel congenital myasthenia syndrome αC418W nicotinic receptor mutation with changes in lipid raft components.

Lipid rafts, specialized membrane microdomains in the plasma membrane rich in cholesterol and sphingolipids, are hot spots for a number of important cellular processes. The novel nicotinic acetylcholine receptor (nAChR) mutation αC418W, the first lipid-exposed mutation identified in a patient that causes slow channel congenital myasthenia syndrome was shown to be cholesterol-sensitive and to accumulate in microdomains rich in the membrane raft marker protein caveolin-1. The objective of this study is to gain insight into the mechanism by which lateral segregation into specialized raft membrane microdomains regulates the activable pool of nAChRs. We performed fluorescent recovery after photobleaching (FRAP), quantitative RT-PCR, and whole cell patch clamp recordings of GFP-encoding Mus musculus nAChRs transfected into HEK 293 cells to assess the role of cholesterol and caveolin-1 (CAV-1) in the diffusion, expression, and functionality of the nAChR (WT and αC418W). Our findings support the hypothesis that a cholesterol-sensitive nAChR might reside in specialized membrane microdomains that upon cholesterol depletion become disrupted and release the cholesterol-sensitive nAChRs to the pool of activable receptors. In addition, our results in HEK 293 cells show an interdependence between CAV-1 and αC418W that could confer end plates rich in αC418W nAChRs to a susceptibility to changes in cholesterol levels that could cause adverse drug reactions to cholesterol-lowering drugs such as statins. The current work suggests that the interplay between cholesterol and CAV-1 provides the molecular basis for modulating the function and dynamics of the cholesterol-sensitive αC418W nAChR.

Del empleo al post-empleo: o de la plasticidad de la psicología en la producción de la subjetividad laboral / From employment to post-employment: on the plasticity of psychology as regards the production of workers' subjectivity / Do emprego para o pós-emprego: ou da plasticidade da psicologia na produção de subjetividade laboral

En este artículo se considera la re-organización del "complejo psi", en relación a las transformaciones que ocurren contemporáneamente en el mundo del trabajo. Para esto se hace un recorrido examinando los cambios en el lugar y las operaciones de la psicología con respecto a las formas sociales empleo y post-empleo. Se argumenta que el papel de la psicología en relación a la producción de la subjetividad laboral se ha ido transformando en medio a los cambios que ávidamente han ido socavando lo que ahora se conoce como el "buen trabajo". Estas mutaciones se caracterizan por el aumento acelerado de incertidumbres relacionadas con los distintos aspectos de las condiciones laborales tales como, la contratación, los horarios, los espacios laborales, y las relaciones interpersonales. Se concluye que, en este contexto, la psicología juega un papel preponderante para la producción de los nuevos sujetos que requiere la organización capitalista del trabajo.

In this article the re-organization of the "psy complex" is considered as regards the contemporary transformations that are taking place in the world of work. Toward this end, a review of the changes in the place and operations of psychology with respect to the employment and post-employment social forms is conducted. It is argued that the role of psychology in the production of workers' subjectivity is transforming amidst the mutations that have been avidly debilitating what is at present known as "good work".These mutations are characterized by an increasing rate of incertitude in aspects such as work contracts, rosters, labor spaces, and interpersonal relationships. It is concluded that in this context, psychology plays a central role for the production of new subjects that the capitalist organization of work requires.

Neste artigo, a reorganização do "complexo psi" é considerada em relação às transformações que estão ocorrendo no mundo do trabalho contemporâneo. Para isso, examinam-se as mudanças ocorridas com o ambiente e as operações da psicologia em relação às formas sociais de emprego e pós-emprego. Argumenta-se que o papel da psicologia em relação à produção da subjetividade laboral modifica-se em meio às transformações que, avidamente, vêm debilitando o que agora se conhece como o "bom trabalho". Essas mutações são caracterizadas pelo aumento acelerado das incertezas relacionadas com diferentes aspectos das condições de trabalho, como contratação, horários, espaços de trabalho e relações interpessoais. Conclui-se que, nesse contexto, a psicologia desempenha papel importante para a produção de novos sujeitos requeridos pela organização capitalista do trabalho.

Epigenetic mechanisms, T-cell activation, and CCR5 genetics interact to regulate T-cell expression of CCR5, the major HIV-1 coreceptor.

T-cell expression levels of CC chemokine receptor 5 (CCR5) are a critical determinant of HIV/AIDS susceptibility, and manifest wide variations (i) between T-cell subsets and among individuals and (ii) in T-cell activation-induced increases in expression levels. We demonstrate that a unifying mechanism for this variation is differences in constitutive and T-cell activation-induced DNA methylation status of CCR5 cis-regulatory regions (cis-regions). Commencing at an evolutionarily conserved CpG (CpG -41), CCR5 cis-regions manifest lower vs. higher methylation in T cells with higher vs. lower CCR5 levels (memory vs. naïve T cells) and in memory T cells with higher vs. lower CCR5 levels. HIV-related and in vitro induced T-cell activation is associated with demethylation of these cis-regions. CCR5 haplotypes associated with increased vs. decreased gene/surface expression levels and HIV/AIDS susceptibility magnify vs. dampen T-cell activation-associated demethylation. Methylation status of CCR5 intron 2 explains a larger proportion of the variation in CCR5 levels than genotype or T-cell activation. The ancestral, protective CCR5-HHA haplotype bears a polymorphism at CpG -41 that is (i) specific to southern Africa, (ii) abrogates binding of the transcription factor CREB1 to this cis-region, and (iii) exhibits a trend for overrepresentation in persons with reduced susceptibility to HIV and disease progression. Genotypes lacking the CCR5-Δ32 mutation but with hypermethylated cis-regions have CCR5 levels similar to genotypes heterozygous for CCR5-Δ32. In HIV-infected individuals, CCR5 cis-regions remain demethylated, despite restoration of CD4+ counts (≥800 cells per mm(3)) with antiretroviral therapy. Thus, methylation content of CCR5 cis-regions is a central epigenetic determinant of T-cell CCR5 levels, and possibly HIV-related outcomes.

Cockroach sensitization mitigates allergic rhinoconjunctivitis symptom severity in patients allergic to house dust mites and pollen.

BACKGROUND: Modifiers of symptom severity in patients with allergic rhinoconjunctivitis (AR) are imprecisely characterized. The hygiene hypothesis implicates childhood microbial exposure as a protective factor. Cockroach sensitization (C+) might be a proxy for microbial exposure. OBJECTIVE: We sought to determine whether C+ assayed by means of skin prick tests influenced AR symptom severity in controlled and natural settings. METHODS: Total symptom scores (TSSs) were recorded by 21 participants with house dust mite allergy (M+) in the natural setting and during repeated exposures of 3 hours per day to house dust mite allergen in an allergen challenge chamber (ACC). In M+ participants the peripheral blood and nasal cells were assayed for T-cell activation and transcriptomic profiles (by using RNA sequencing), respectively. Participants allergic to mountain cedar (n = 21), oak (n = 34), and ragweed (n = 23) recorded TSSs during separate out-of-season exposures to these pollens (any pollen sensitization [P+]) in the ACC; a subset recorded TSSs in the pollination seasons. RESULTS: The hierarchy of TSSs (highest to lowest) among M+ participants tracked the following skin prick test sensitization statuses: M+P+C- > M+P+C+ > M+P-C- > M+P-C+. In nasal cells and peripheral blood the immune/inflammatory responses were rapidly resolved in M+P+C+ compared with M+P+C- participants. Among those allergic to pollen, C+ was associated with a lower TSS during pollen challenges and the pollination season. After aggregated analysis of all 4 ACC studies, C+ status was associated with a 2.8-fold greater likelihood of a lower TSS compared with C- status (odds ratio, 2.78; 95% CI, 1.18-6.67; P = .02). CONCLUSIONS: C+ status is associated with mitigation of AR symptom severity in adults with AR.

El "museo de la psicología" como estrategia para la construcción de la historia oral de la psicología / The "Museum of Psychology" as a Strategy for the Construction of the Oral History of Psychology

En este artículo, se presenta la construcción de un "museo de la psicología" como estrategia para la elaboración de la historia oral de la disciplina. En primer lugar, el debate entre psicología e historia constituye el marco para considerar el puesto que pueden ocupar los relatos de la experiencia de la práctica psicológica, en relación con la constitución de la historia de esta disciplina. A continuación, se describen los elementos fundamentales de la estrategia llamada "museo de la psicología", diseñada para elicitar historias orales. Para finalizar, a manera de ilustración, se consideran ventajas y limitaciones que emergen de la aplicación de la estrategia.

In this article the construction of a "museum of psychology" is presented as a strategy for the elaboration of the oral history of the discipline. First of all, the debate between history and psychology constitutes the framework to consider the place of psychological practices as regards the construction of the history of psychology. Then, the fundamental elements of the strategy called "the museum of psychology" are introduced. Finally, as an illustration, the advantages and the limitations of the strategy are presented.

Generalizing thermodynamic properties of bulk single-walled carbon nanotubes.

The enthalpy and Gibbs free energy thermodynamical potentials of single walled carbon nanotubes were studied of all types (armchairs, zig-zags, chirals (n>m), and chiral (n

Y entonces ¿esto de la crítica qué es? De la relaciones entre la psicología y el mundo del trabajo / So, What is Critique? On the Relationships between Psychology and the World of Work

En este artículo se consideran diversas maneras en las cuales se han formulado objeciones a la relación que la psicología ha establecido con el mundo del trabajo. Se revisan las principales corrientes críticas, para establecer el foco de análisis de sus propuestas y los objetivos que persiguen al examinar dicha relación. La discusión aporta elementos para constituir una actitud de balance que difiera de aquellas posiciones catastróficas que ven en la psicología un saber que promueve el control y la subordinación y aquellas posiciones optimistas que encuentran en este conocimiento estrategias para el progreso, el bienestar y la emancipación.

This article considers different ways in which have been formulated objections to the relationship established between psychology and the world of work. The main perspectives that propose critiques are revisited to establish the core analyses and their main objectives pursued. The discussion aims to contribute to the construction of an attitude of balance that differs from those catastrophic perspectives which evaluate psychology as a knowledge that promotes control and subordination and those optimistic positions which find in this knowledge some strategies for progress, wellbeing and emancipation.

Rab1b overexpression modifies Golgi size and gene expression in HeLa cells and modulates the thyrotrophin response in thyroid cells in culture.

Rab1b belongs to the Rab-GTPase family that regulates membrane trafficking and signal transduction systems able to control diverse cellular activities, including gene expression. Rab1b is essential for endoplasmic reticulum-Golgi transport. Although it is ubiquitously expressed, its mRNA levels vary among different tissues. This work aims to characterize the role of the high Rab1b levels detected in some secretory tissues. We report that, in HeLa cells, an increase in Rab1b levels induces changes in Golgi size and gene expression. Significantly, analyses applied to selected genes, KDELR3, GM130 (involved in membrane transport), and the proto-oncogene JUN, indicate that the Rab1b increase acts as a molecular switch to control the expression of these genes at the transcriptional level, resulting in changes at the protein level. These Rab1b-dependent changes require the activity of p38 mitogen-activated protein kinase and the cAMP-responsive element-binding protein consensus binding site in those target promoter regions. Moreover, our results reveal that, in a secretory thyroid cell line (FRTL5), Rab1b expression increases in response to thyroid-stimulating hormone (TSH). Additionally, changes in Rab1b expression in FRTL5 cells modify the specific TSH response. Our results show, for the first time, that changes in Rab1b levels modulate gene transcription and strongly suggest that a Rab1b increase is required to elicit a secretory response.