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1.
J Mater Sci Mater Med ; 27(3): 52, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26787484

RESUMEN

In implantology, as an alternative approach to the use of antibiotics, direct surface modifications of the implant addressed to inhibit bacterial adhesion and to limit bacterial proliferation are a promising tactic. The present study evaluates in an in vivo normal model the osteogenic response and the osteointegration of an anodic spark deposition nanostructured titanium surface doped with gallium (ASD + Ga) in comparison with two other surface treatments of titanium: an anodic spark deposition treatment without gallium (ASD) and an acid etching treatment (CTR). Moreover the study assesses the osteoprotective potential and the antibacterial effect of the previously mentioned surface treatments in an experimentally-induced peri-implantitis model. The obtained data points out a more rapid primary fixation in ASD and ASD + Ga implants, compared with CTR surface. Regarding the antibacterial properties, the ASD + Ga surface shows osteoprotective action on bone peri-implant tissue in vivo as well as an antibacterial effect within the first considered time point.


Asunto(s)
Nanoestructuras/química , Osteogénesis , Titanio , Animales , Fracturas del Fémur/patología , Fracturas del Fémur/cirugía , Galio/efectos adversos , Galio/química , Masculino , Ensayo de Materiales , Microscopía Electrónica de Rastreo , Prótesis e Implantes , Infecciones Relacionadas con Prótesis/patología , Conejos , Propiedades de Superficie , Ingeniería de Tejidos/métodos
2.
Vet Comp Orthop Traumatol ; 28(5): 347-54, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26037208

RESUMEN

OBJECTIVE: To report clinical experiences with the tibial plateau levelling osteotomy (TPLO) procedure in small breed dogs with cranial cruciate ligament (CCL) disease using specific, conically coupled, 1.9/2.5 mm locking plates and evaluating short-term complications and outcome. METHODS: Medical records of small breed dogs (<15 kg) that underwent TPLO using 1.9/2.5 mm locking plates were reviewed retrospectively. The preoperative, postoperative and six to eight weeks postoperative tibial plateau angle (TPA) measurements were determined from the radiographic images. Lameness evaluation was assessed subjectively preoperatively and six to eight weeks postoperatively. RESULTS: Sixty-nine small breed dogs (n = 79 stifles) were included in the study. Mean (± SD) preoperative TPA was 29.0 ± 3.4°, postoperative TPA was 5.8 ± 2.5°, and six to eight weeks postoperative TPA was 7.3 ± 4.1°. Sixteen complications occurred in 12 out of 79 TPLO procedures: three were intra-operative (intra-articular screw placement) and 13 were postoperative complications, of which nine were identified as minor complications not requiring surgical reintervention, and four as major complications requiring additional surgical intervention, including tibial tuberosity fracture (n = 1), osteomyelitis (n = 1), screw failure (n = 1), and plate breakage (n = 1). Lameness scores by clinical assessment reduced from a median value of 3/4 preoperatively to 1/4 at six to eight weeks postoperatively. CLINICAL SIGNIFICANCE: 1.9/2.5 mm locking plates appear to be a valid choice of implant for the stabilization of unilateral TPLO in small breed dogs.


Asunto(s)
Placas Óseas/veterinaria , Perros/cirugía , Osteotomía/veterinaria , Tibia/cirugía , Animales , Ligamento Cruzado Anterior/cirugía , Tornillos Óseos/veterinaria , Femenino , Masculino , Osteotomía/efectos adversos , Osteotomía/métodos , Radiografía , Estudios Retrospectivos , Tibia/diagnóstico por imagen
3.
Acta Biomater ; 6(3): 1014-24, 2010 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-19800423

RESUMEN

Anodic spark deposition (ASD) is an attractive technique for improving the implant-bone interface that can be applied to titanium and titanium alloys. This technique produces a surface with microporous morphology and an oxide layer enriched with calcium and phosphorus. The aim of the present study was to investigate the biological response in vitro using primary human osteoblasts as a cellular model and the osteogenic primary response in vivo within a short experimental time frame (2 and 4 weeks) in an animal model (rabbit). Responses were assessed by comparing the new electrochemical biomimetic treatments to an acid-etching treatment as control. The in vitro biological response was characterized by cell morphology, adhesion, proliferation activity and cell metabolic activity. A complete assessment of osteogenic activity in vivo was achieved by estimating static and dynamic histomorphometric parameters at several time points within the considered time frame. The in vitro study showed enhanced osteoblast adhesion and higher metabolic activity for the ASD-treated surfaces during the first days after seeding compared to the control titanium. For the ASD surfaces, the histomorphometry indicated a higher mineral apposition rate within 2 weeks and a more extended bone activation within the first week after surgery, leading to more extensive bone-implant contact after 2 weeks. In conclusion, the ASD surface treatments enhanced the biological response in vitro, promoting an early osteoblast adhesion, and the osteointegrative properties in vivo, accelerating the primary osteogenic response.


Asunto(s)
Galvanoplastia/métodos , Fracturas del Fémur/patología , Fracturas del Fémur/cirugía , Osteoblastos/fisiología , Osteoblastos/trasplante , Osteogénesis/fisiología , Ingeniería de Tejidos/métodos , Titanio/química , Animales , Preescolar , Cristalización/métodos , Humanos , Ensayo de Materiales , Osteoblastos/patología , Conejos , Resultado del Tratamiento
5.
Vet Comp Orthop Traumatol ; 18(4): 243-5, 2005.
Artículo en Inglés | MEDLINE | ID: mdl-16594393

RESUMEN

Incomplete humeral condylar fracture was diagnosed by means of radiology, CT, scintigraphy, arthroscopy and bone biopsy in two English Pointer dogs. In both cases an acute thoracic limb lameness, unrelated to a known episode of major trauma, was observed. Incomplete humeral condylar fracture, mainly described in the Spaniel breeds, has been recently diagnosed in Labrador retrievers, Rottweiler, German Wachtel and other breeds. The pathogenesis of the condition is still unknown, but incomplete ossification of the humeral condyle and mechanical stress, alone or associated, have to be considered. However, our clinical and histopathological data lead us to believe that in Pointers, high performance dogs, the mechanical stress can assume a critical ethiopathogenetic role.


Asunto(s)
Perros/lesiones , Fracturas del Húmero/veterinaria , Estrés Mecánico , Animales , Tornillos Óseos/veterinaria , Perros/genética , Perros/cirugía , Femenino , Predisposición Genética a la Enfermedad , Fracturas del Húmero/diagnóstico , Fracturas del Húmero/diagnóstico por imagen , Fracturas del Húmero/cirugía , Cojera Animal/etiología , Masculino , Radiografía
7.
J Small Anim Pract ; 43(1): 7-11, 2002 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-11833823

RESUMEN

A modified technique is presented for surgical and diagnostic arthroscopy of the shoulder joint in the dog. The technique involves access to the joint through two points only; one was created in place of the drainage needle-cannula, which was replaced with a portal, while the second was located more caudally compared with previous techniques. Using a changing guide rod system the two portals are completely interchangeable in order to perform easier arthroscopic surgery either in the cranial or caudal aspect of the joint. The presence of only one portal caudal to the lateral collateral ligament allows more freedom of movement and avoids interference between the arthroscope and the instruments. The modified procedure was performed on 33 joints affected by osteochondritis dissecans or tenoligament diseases and facilitated straightforward diagnostic examinations, and simple and rapid surgical procedures.


Asunto(s)
Artroscopía/veterinaria , Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/cirugía , Osteocondritis Disecante/veterinaria , Articulación del Hombro/cirugía , Tendinopatía/veterinaria , Animales , Artroscopía/métodos , Cruzamiento , Perros , Femenino , Ligamentos/cirugía , Masculino , Osteocondritis Disecante/diagnóstico , Osteocondritis Disecante/cirugía , Cirugía Veterinaria/métodos , Tendinopatía/diagnóstico , Tendinopatía/cirugía
8.
Vet Surg ; 30(5): 468-75, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11555823

RESUMEN

OBJECTIVE: To evaluate the effectiveness of an extra-articular surgical technique using absorbable suture material for the stabilization of traumatic coxofemoral luxation in dogs. STUDY DESIGN: Prospective, clinical study. ANIMALS: Fourteen client-owned dogs with recent and long-standing traumatic coxofemoral luxation (13 craniodorsal and 1 ventral). METHODS: Coxofemoral luxations were surgically reduced and maintained in place with an extra-articular iliofemoral multifilamentous absorbable suture (3 to 6 strands of 2 USP Polyglactin 910). No external support was employed, and all the dogs were encouraged to use the affected limb postoperatively. The average time of clinical and radiographic follow-up was 11.6 +/- 6.3 months (from 2 to 22 months). RESULTS: During the follow-up period, no reluxations occurred and no complications associated with the surgical technique were identified. The dogs started bearing weight from 1 to 10 days after the surgery (mean, 4.3 +/- 2.9 days) and the period of lameness ranged from 7 to 30 days (20 +/- 8.6 days). At the final clinical examination, the dogs did not demonstrate any lameness or pain during passive flexo-extension movements, and there was no significant limitation of the range of motion. CONCLUSION: Extra-articular stabilization with multifilamentous absorbable sutures is a simple, effective method of treatment for acute and chronic coxofemoral luxation. The absorbable material used is strong enough to maintain articular stability during the period of scar tissue formation even in large-breed dogs. CLINICAL RELEVANCE: Absorbable sutures avoid the possible complications related to the use of nonabsorbable material and seem to be sufficient to maintain articular stability during the capsular healing process.


Asunto(s)
Perros/lesiones , Luxación de la Cadera/veterinaria , Técnicas de Sutura/veterinaria , Implantes Absorbibles/veterinaria , Animales , Cruzamiento , Perros/cirugía , Femenino , Luxación de la Cadera/cirugía , Masculino , Complicaciones Posoperatorias/veterinaria , Estudios Prospectivos , Técnicas de Sutura/instrumentación , Suturas
9.
J Exp Biol ; 134: 281-95, 1988 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-3356964

RESUMEN

Chromatophore motoneurones in Lolliguncula brevis are known to originate in the suboesophageal lobes of the brain and to project directly to the mantle and fin through bilateral stellate ganglia and fin nerves. The chromatophore motor fields of stellar and fin nerves were investigated by stimulation of the cut end of individual nerves in a semi-intact preparation. This elicited expansion of yellow and brown chromatophores in distinct motor fields. Brown chromatophores extended over the entire mantle, whereas yellow chromatophores were limited to the dorsal and lateral mantle areas. Combined nerve stimulation and lesions demonstrated substantial overlap between adjacent chromatophore motor fields and innervation of individual chromatophores by different stellar nerves.


Asunto(s)
Cromatóforos/fisiología , Decapodiformes/fisiología , Animales , Encéfalo/fisiología , Estimulación Eléctrica , Ganglios/fisiología , Neuronas Motoras/fisiología
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