Antibiotics prescribing habits of Brazilian general dental practitioners during periodontal treatments / Hábitos de prescrição de antibióticos de dentistas brasileiros durante tratamentos periodontais

Introdução: O tratamento periodontal envolve procedimentos que visam reduzir a carga bacteriana, envolvendo ou não o uso de antibióticos locais ou sistêmicos. Objetivo: O objetivo deste estudo foi avaliar o conhecimento dos cirurgiões-dentistas brasileiros sobre a prescrição de antibióticos durante o tratamento periodontal. Material e método: Foi disponibilizado um questionário online solicitando aplicação sistêmica de prescrição de antibióticos locais ou sistêmicos (frequência e sequência com tratamento periodontal mecânico) e em relação a diferentes doenças periodontais. A utilização de serviços de diagnóstico microbiano e dados demográficos dos voluntários também foram elucidados. Os dados foram analisados estatisticamente (Teste Binomial, p<0,05). Resultado: Trezentos e noventa e três voluntários responderam ao questionário. Vinte e três por cento do sexo masculino e 76,2% do sexo feminino. A idade média foi de 27,7 anos. A minoria (19,2%) dos voluntários relatou indicar antibióticos sistêmicos para tratamento de gengivite ou periodontite estágios I e II (antiga periodontite crônica leve e moderada). A maioria dos profissionais indica para tratar abscesso periodontal, gengivite ou periodontite ulcerativa necrosante, periodontite em imunodeficiências graves, periodontite agressiva antiga e periodontite estágios III e IV (periodontite crônica grave antiga). Raramente (2,5%) a prescrição foi sem a associação com terapia mecânica. Os exames microbiológicos como método auxiliar de diagnóstico raramente (3,1%) são solicitados por falta de informação ou custo. As respostas mais frequentes sobre o número de vezes que os profissionais relataram prescrever antibióticos sistêmicos como parte do tratamento periodontal por trimestre foram "pelo menos uma vez", seguido de "2 vezes". Conclusão: Pode-se concluir que os cirurgiões-dentistas brasileiros possuem conhecimento sobre a correta prescrição de antibióticos na terapia periodontal

Introduction: Periodontal treatment involves procedures aimed to reduce bacterial load, involving or not the use of local or systemic antibiotics. Objective: The purpose of this study was to assess Brazilian dentists' knowledge about antibiotics prescription during periodontal treatment. Material and method: An online questionnaire was available asking for systemic application of local or systemic antibiotics prescription (frequency, and sequence with mechanical periodontal treatment) and in relation to different periodontal diseases. The use of microbial diagnostic services and volunteers' demographic data were also elucidated. Data were statistically analyzed (Binomial Test, p<0.05). Result: Three hundred and ninety-three volunteers answered the questionnaire. Twenty-three percent males and 76.2% females. Average age was 27.7 years. Minority (19.2%) of the volunteers reported to indicate systemic antibiotics to treat gingivitis or periodontitis stages I and II (old slight and moderate chronic periodontitis). Majority of the professionals indicate it to treat periodontal abscess, necrotizing ulcerative gingivitis or periodontitis, periodontitis in severe immunodeficiencies, old aggressive periodontitis, and periodontitis stages III and IV (old severe chronic periodontitis). Rarely (2.5%) the prescription was without the combination with mechanical therapy. Microbiological tests as an auxiliary diagnostic method are rarely (3.1%) requested due to lack of information or cost. Most frequent answers about the number of times professionals reported prescribing systemic antibiotics as part of periodontal treatment per trimester was "at least once", followed by "2 times". Conclusion: It can be concluded that Brazilian dentists have knowledge about the correct prescription of antibiotics in periodontal therapy

miR-20a is upregulated in serum from domestic feline with PKD1 mutation.

Polycystic kidney disease (PKD), also known as autosomal dominant polycystic kidney disease (ADPKD) is a genetically heterogeneous condition characterized by cysts in renal parenchyma. It is the most prevalent inherited disease of domestic cats. MicroRNAs (miRNAs or ncRNA) are short, noncoding, single-stranded RNAs that may induce PKD cytogenesis by affecting numerous targets genes as well as by directly regulating PKD gene expression. We compared the relative expression profile of miR-20a, -192, -365, -15b-5p, and -16-5p from plasma and serum samples of nine domestic cats with PKD1 mutation, detected by polymerase chain reaction (PCR), and a control group (n = 10). Blood samples from cats with PKD1 mutation provide similar concentrations of microRNAs either from plasma or serum. Serum miR-20a is upregulated in PKD group with p < 0.005; Roc curve analysis showed an AUC of 90,1% with a cut-off value sensitivity of 77.8% and specificity of 100%. This data provides important information regarding renal miRNA expression in peripheral blood sampling.

Survey based assessment of diagnosis through periapical radiograph and CBCT and treatment of root resorption with Brazilian and American dentists and endodontists.

BACKGROUND: This study assesses and compares the knowledge level of endodontists (ENDs) and general dental practitioners (GPs) from Brazil and United States of America (USA) in the diagnosis and treatment of internal and external inflammatory root resorptions through periapical radiographic (PA) and cone beam computed tomography (CBCT) examinations. MATERIAL AND METHODS: A cross-sectional online questionnaire-based survey was presented to the volunteers containing questions regarding personal and professional profile, as well as three clinical cases of internal and external inflammatory root resorption. A series of multiple-choice questions about the diagnosis and treatment options were surveyed. The data collected was analysed by the Chi-square test with Yates correction with a significance level of 5 %. RESULTS: Most answers were considered adequate when all three questions about the diagnosis and all two questions relating to the treatment were answered accurately. A total of 374 dentists answered the survey (n: 229 from Brazil vs. 145 from USA) being 41% END and 59% GP. END presented higher level of knowledge than GP regarding to diagnosis and treatment of inflammatory root resorptions both in Brazil and USA (p<0.05); USA presented higher level of adequate responses than Brazil (p<0.05). CONCLUSIONS: END achieved a level of knowledge of the diagnosis and treatment of root resorption superior to the GP. Comparing the results obtained in both countries, it was observed that the USA had a higher correct response rate than Brazil. Key words:Internal root resorption, external root resorption, management, diagnosis, treatment.

RT-rtPCR quantification of circulating microRNAs in plasma and serum samples from healthy domestic cats.

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at a post-transcriptional level by silencing targeted messenger RNA (mRNA). Most studies concerning miRNA expression use solid tissue samples. However, circulating miRNAs from different body fluids have recently emerged as diagnostic and prognostic molecules, given that they hold informative value and have increased stability in cell-free form. Blood sampling of cats can be challenging given their small body size and because they often experience distress when handled. We quantified miR-20a, -192, -365, -15b-5p, and -16-5p from plasma and serum samples of 10 healthy domestic cats. Our RT-rtPCR procedure used 100 µL of either plasma or serum samples as sources of biomarker molecules. However, serum provided higher amounts of miRNA than plasma samples, with a p < 0.0001 for miR-20a and p < 0.0002 for miR-16-5p.

Biomechanical effect of an occlusal device for patients with an implant-supported fixed dental prosthesis under parafunctional loading: A 3D finite element analysis.

STATEMENT OF PROBLEM: Whether providing an occlusal device for a patient with bruxism and an implant-supported fixed dental prosthesis leads to improved biomechanics is unclear. PURPOSE: The purpose of this 3D finite element analysis (FEA) study was to evaluate the biomechanical behavior of 3-unit implant-supported prostheses under parafunctional forces with and without an occlusal device. MATERIALS AND METHODS: Eight 3D models consisting of a posterior (type IV) maxillary bone block with 3 external hexagon implants (Ø4.0×7.0 mm) and 3-unit implant-supported prostheses with different crown connections (splinted or unsplinted) and an occlusal device under functional and parafunctional loading were simulated. The abutment screws were evaluated by von Mises stress maps, and the bone tissue by maximum principal stress and microstrain maps by using a finite element software program. RESULTS: An occlusal device improved the biomechanical behavior of the prostheses by reducing stress in the abutment screws and stress and strain in the bone tissue. However, the use of an occlusal device was not sufficiently effective to negate the biomechanical benefit of splinting. CONCLUSIONS: The use of splinted crowns in the posterior maxillary region with an occlusal device was the most effective method of reducing stress in the abutment screws and stress and strain in the bone tissue when parafunction was modeled.

Influence of the Vehicle on the Tissue Reaction and Biomineralization of Fast Endodontic Cement

ABSTRACT Objective: To investigate the tissue response and the biomineralization ability of CER prepared with epoxy resin or water compared to Mineral Trioxide Aggregate (MTA). Material and Methods: Polyethylene tubes containing materials or empty tubes for control were inserted into the subcutaneous tissues of 30 rats. After 7, 15, 30, 60, and 90 days, the rats were killed and the tubes were removed for analysis using hematoxylin-eosin staining, von Kossa staining, and under polarized light. Inflammation was graded through a score system; the thickness of the fibrous capsule was classified as thin or thick; the biomineralization ability was recorded as present or absent. The results were statistically analyzed using the Kruskal-Wallis test (p<0.05). Results: Histologic analysis performed after 7 and 15 days for CER prepared with epoxy resin or water and for MTA showed moderate inflammation and a thick fibrous capsule (p>0.05). After 30, 60, and 90 days, mild inflammation, and a thin fibrous capsule were observed in all groups (p>0.05). Conclusion: All materials had structures positive for von Kossa and birefringent to polarized light. CER epoxy resin showed biocompatibility and biomineralization similar to CER water and MTA.

Uraemic Encephalopathy in a Persian Cat with Chronic Kidney Disease.

Uraemic encephalopathy (UE) is rarely associated with acute kidney injury or chronic kidney disease in domestic animals, and we now report the first case in a cat. The animal presented with hypothermia, apathy, lethargy, depression, severe dehydration, uraemic breath, elevated serum urea nitrogen and creatine concentrations, and eventual seizures and coma prior to death. Gross necropsy findings included severe bilateral renal scarring, ulcerative stomatitis and glossitis, and uraemic gastropathy. Microscopic lesions of diffuse interstitial fibrosis, multifocal mineralization and lymphoplasmacytic interstitial nephritis were seen in the kidneys. There was symmetrical, bilateral spongy vacuolation of the white matter of the basal nuclei and cerebellum and Alzheimer type II astrocytes in the cerebral cortex and hippocampus. Glial fibrillary acid protein immunolabelling was absent or faint in astrocytes of the cerebral grey matter. UE should be included in the differential diagnosis in animals with chronic kidney disease and neurological signs.

Antimicrobial action of NeoMTA Plus on mono- and dual-species biofilms of Enterococcus faecalis and Candida albicans: An in vitro study.

OBJECTIVE: To evaluate the antimicrobial action of NeoMTA Plus on mono- and dual-species biofilms of Enterococcus faecalis and Candida albicans. MATERIAL AND METHODS: A total of 171 sterile dentin blocks, measuring 4 mm × 4 mm × 1 mm, were incubated in media containing E. faecalis (3.1 × 108 cells/mL) and/or C. albicans (1 × 107 cells/mL) for 2 d. These blocks were randomly divided into three groups: the control (no treatment with biomaterials), MTA (treated with an MTA sample with width and thickness same as those of the dentin block after the material was set), and NeoMTA Plus (treated with NeoMTA Plus in a fashion similar to the treatment of the MTA group) groups. The biomaterials remained in contact with the biofilms for 24 h. Quantitative analyses of the number of colony-forming units (CFUs) and metabolic activity (XTT), were performed. Furthermore, qualitative analysis of biofilm structure was performed by scanning electron microscopy. Data were statistically analyzed considering a significance level of 5%. RESULTS: XTT and the number of CFUs were similar among the groups (p > 0.05). The type of biofilm (mono- or dual-species) or the biomaterial used (MTA or NeoMTA Plus) did not affect the results. Biofilm structure exhibited a robust architecture composed of yeast and bacterial cell multilayers and was homogeneous among the groups. CONCLUSION: NeoMTA Plus was not effective against mono- and dual-species biofilms of E. faecalis and C. albicans. Further research investigating biofilm removal methods including those involving the use of biomaterials with antiseptics and other supporting therapies is warranted.

Post-operative pain after using sodium hypochlorite and chlorhexidine as irrigation solutions in endodontics: Systematic review and meta-analysis of randomised clinical trials.

CONTEXT: Is it possible that the irrigating solutions can have the potential to cause post-operative pain? Unfortunately, the current literature does not provide clear guidance. AIM: The purpose of this systematic review and meta-analysis was to comprehensively review two different irrigation solutions (sodium hypochlorite and chlorhexidine) regarding the post-operative pain after endodontic treatment. SETTINGS AND DESIGN: This study was prepared according to the Cochrane criteria for creating a systematic review and meta-analysis and confirms the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. MATERIAL AND METHODS: This search was conducted in the PubMed/MEDLINE, SCOPUS and Cochrane Library databases until February 2018 to answer the In [(Population) what is the effect of (Intervention) on (Outcome), compared with (Comparison) Intervention] (PICO) question: could sodium hypochlorite cause more post-operative pain than chlorhexidine in teeth subjected to endodontic treatment? The primary outcome was overall post-operative pain after 24 h. RESULTS: After applying the inclusion and exclusion criteria, three randomized clinical trials fulfilled the eligibility criteria, and two were subjected to the meta-analysis. There was no difference in post-operative pain between the tested irrigating solutions. CONCLUSIONS: There are few studies published in the current literature; therefore, additional randomized clinical studies are required to on this topic to help clinicians make the best decision concerning treatment.

Reciprocating kinematics leads to lower incidences of postoperative pain than rotary kinematics after endodontic treatment: A systematic review and meta-analysis of randomized controlled trial.

BACKGROUND: Extrusion of infected debris into the periapical tissue has been cited as the major cause of postoperative pain, regardless of instrumentation technique. AIM: Comprehensively review two different kinematics of instrumentation (reciprocating and rotary) and association to the postoperative pain after endodontic treatment. METHODS: Two investigators performed a systematic review with meta-analysis. MEDLINE/PubMed, Cochrane Library, and Scopus supplied relevant data from studies published until February 2018 to answer the PICO question. Primary outcome was overall postoperative pain, and the secondary outcomes were nature of the pain (mild, moderate, and severe) at 12, 24, and 48 h. RESULTS: Ten randomized clinical trials fulfilled eligibility criteria, and five of them were submitted in the meta-analysis. Primary outcome indicated that reciprocating system results in less postoperative pain compared to rotary system (P < 0.05). As a secondary outcome, there was no statistical difference for mild, moderate, and severe pain after 12 and 24 h using reciprocating or rotary systems (P > 0.05). However, the reciprocation system showed less severe pain after 48 h (P < 0.05). CONCLUSION: Rotary motion had a negative impact on postoperative pain after endodontic treatment. Furthermore, after 48 h, more patients presented severe pain under rotary motion. More randomized clinical studies would be helpful.

Óbitos por violência na capital de Mato Grosso, Brasil: circunstâncias e fatores associados / Deaths from violence in the capital city of Mato Grosso state, Brasil: associated factors and circumstances / Muertes por violencia en la capital del estado de Mato Grosso, Brasil: las circunstancias y los factores asociados

Atualmente, a violência constitui-se grande desafio para o setor saúde, em que crianças, adolescentes e jovens têm sido reconhecidos como grupos etários de maior vulnerabilidade, o que desperta para a necessidade de entendimento das circunstâncias e dos fatores de risco neste grupo específico. O estudo teve o objetivo de identificar os fatores associados aos óbitos por violência ocorridos na capital de Mato Grosso, em 2009, no grupo de 0 a 24 anos de idade. A metodologia adotada foi o inquérito domiciliar, cujos dados foram analisados pelo EpiInfo, sendo o valor de p considerado de <0,05 para significância estatística. Foram estudados 82 óbitos: 73 por agressão (89,0%), 7 por lesão autoprovocada (8,5%) e 2 por intervenção legal (2,4%). Os resultados mostraram: quanto às vulnerabilidades, maternidade na adolescência, sobrecarga de papéis maternos, consumo de álcool e drogas, conflitos familiares, episódios de violência e morte anterior por violência na família; quanto aos fatores de proteção, o acesso à rede de saúde foi relatado como afirmativo. Concluiu-se que a presença de situações de vulnerabilidade e a ausência de fatores de proteção, bem como a concepção sobre a forma de educar os filhos, apontaram para a fragilidade das famílias estudadas no que se refere à violência.

Currently, violence constitutes a great challenge for the health sector, as children, adolescents and young people have been recognized as the most vulnerable age groups, which points at the importance of understanding the circumstances and the risk factors for this particular group. The study sought to identify factors associated with violent deaths in the capital of Mato Grosso, in 2009, from the group 0-24 years of age. It was adopted a household survey, whose data were analyzed using EpiInfo, with the value of p considered <0.05 for statistical significance. 82 deaths were studied: 73 by aggression (89.0%), 7 by self-harm (8.5%) and 2 by legal intervention (2.4%). Results show: regarding vulnerabilities, teenage motherhood, maternal roles overload, use of alcohol and drugs, family conflicts, previous episodes of violence and death from violence in the family; regarding protective factors, access to health care services was reported as affirmative. In conclusion, the presence of vulnerability situations and the absence of protective factors, as well as the conception on how to raise children, pointed to the fragility of the families studied regarding violence.

Actualmente, la violencia constituye un gran desafío para el sector de la salud, en que los niños, adolescentes y jóvenes han sido reconocidos como los grupos de edad más vulnerables, lo que despierta para la importancia de comprender las circunstancias y los factores de riesgo en este grupo particular. El estudio trató de identificar los factores asociados a las muertes violentas, que se produjeron en la capital de Mato Grosso, en 2009, en el grupo de 0-24 años de edad. La metodología adoptada fue la encuesta de hogares, cuyos datos fueron analizados utilizando EpiInfo, con el valor de p considerado <0,05 para la significación estadística. Se estudiaron 82 muertes: 73 por agresión (89,0%), 7 de autolesiones (8,5%) y 2 por intervención legal (2,4%). Los resultados mostraron: en cuanto a las vulnerabilidades, maternidad adolescente, sobrecarga de la ocupación materna, consumo de alcohol y drogas, conflictos familiares, episodios de violencia y muerte anterior a causa de la violencia en la familia; en cuanto a los factores de protección, el acceso a los servicios de salud fue reportado como afirmativo. Concluyese que la presencia de situaciones de vulnerabilidad y la ausencia de factores de protección, así como la concepción de cómo criar a los niños, apuntan a la fragilidad de las familias estudiadas en relación con la violencia.

Dragon's Blood Sap (Croton Lechleri) As Storage Medium For Avulsed Teeth: In Vitro Study Of Cell Viability.

Tooth replantation success depends on the condition of cementum periodontal ligament after tooth avulsion; which is influenced by storage medium. The dragon's blood (Croton lechleri) sap has been suggested as a promising medium because it supports collagen formation and exhibits healing, anti-inflammatory and antimicrobial properties. Thus, the aim of this study was to evaluate the efficacy of dragon's blood sap as a storage medium for avulsed teeth through evaluation of functional and metabolic cell viability. This in vitro study compared the efficacy of different storage media to maintain the viability of human peripheral blood mononuclear and periodontal ligament cells. A 10% dragon's blood sap was tested while PBS was selected as its control. Ultra pasteurized whole milk was used for comparison as a commonly used storage medium. DMEM and distilled water were the positive and negative controls, respectively. The viability was assessed through trypan blue exclusion test and colorimetric MTT assay after 1, 3, 6, 10 and 24 h of incubation. The dragon's blood sap showed promising results due to its considerable maintenance of cell viability. For trypan blue test, the dragon's blood sap was similar to milk (p<0.05) and both presented the highest viability values. For MTT, the dragon's blood sap showed better results than all storage media, even better than milk (p<0.05). It was concluded that the dragon's blood sap was as effective as milk, the gold standard for storage medium. The experimental sap preserved the membrane of all cells and the functional viability of periodontal ligament cells.

Dragon's Blood Sap (Croton Lechleri) As Storage Medium For Avulsed Teeth: In Vitro Study Of Cell Viability

Abstract Tooth replantation success depends on the condition of cementum periodontal ligament after tooth avulsion; which is influenced by storage medium. The dragon's blood (Croton lechleri) sap has been suggested as a promising medium because it supports collagen formation and exhibits healing, anti-inflammatory and antimicrobial properties. Thus, the aim of this study was to evaluate the efficacy of dragon's blood sap as a storage medium for avulsed teeth through evaluation of functional and metabolic cell viability. This in vitro study compared the efficacy of different storage media to maintain the viability of human peripheral blood mononuclear and periodontal ligament cells. A 10% dragon's blood sap was tested while PBS was selected as its control. Ultra pasteurized whole milk was used for comparison as a commonly used storage medium. DMEM and distilled water were the positive and negative controls, respectively. The viability was assessed through trypan blue exclusion test and colorimetric MTT assay after 1, 3, 6, 10 and 24 h of incubation. The dragon's blood sap showed promising results due to its considerable maintenance of cell viability. For trypan blue test, the dragon's blood sap was similar to milk (p<0.05) and both presented the highest viability values. For MTT, the dragon's blood sap showed better results than all storage media, even better than milk (p<0.05). It was concluded that the dragon's blood sap was as effective as milk, the gold standard for storage medium. The experimental sap preserved the membrane of all cells and the functional viability of periodontal ligament cells.

Resumo O sucesso do reimplante dentário depende da condição apresentada pelo ligamento periodontal cementário pós-avulsão que pode ser influenciado pelo meio de estocagem. O Sangue de Dragão (Croton lechleri) é sugerido como um meio promissor por auxiliar na formação de novo colágeno e apresentar propriedades cicatrizante, anti-inflamatória, antimicrobiana. Assim, o objetivo deste trabalho foi avaliar a eficácia da seiva Sangue de Dragão como meio de estocagem para dentes avulsionados por meio da aferição da viabilidade funcional e metabólica celular. Este estudo in vitro comparou a eficácia dos meios para a manutenção da viabilidade das células mononucleares de sangue periférico humano e do ligamento periodontal mantidas em cultura. Foi testada a diluição a 10% da seiva Sangue de Dragão enquanto que a PBS foi selecionada como seu controle. O leite ultrapasteurizado integral foi utilizado como meio comparativo por ser tradicionalmente utilizado como meio de estocagem. O DMEM e a água destilada foram os controles positivos e negativos, respectivamente. A avaliação da viabilidade foi feita por meio dos testes de exclusão por Azul de Tripan e colorimétrica a base de tetrazolato (MTT), após 1, 3, 6, 10 e 24 h de incubação. A seiva Sangue de Dragão apresentou resultados promissores devido à sua considerável manutenção da viabilidade celular. Para a metodologia com o Azul de Tripan, a seiva Sangue de Dragão foi semelhante ao leite.

Relationship between hypertension and periapical lesion: an in vitro and in vivo study.

The aim of this study was to compare potential aspects of periapical lesion formation in hypertensive and normotensive conditions using hypertensive (BPH/2J) and wild-type control (BPN/3J) mice. The mandibular first molars of both strains had their dental pulp exposed. At day 21 the mice were euthanized and right mandibular molars were used to evaluate the size and phenotype of apical periodontitis by microCT. Proteins were extracted from periapical lesion on the left side and the expressions of IL1α, IL1ß and TNFα were analyzed by ELISA. Bone marrow stem cells were isolated from adult mice femurs from 2 strains and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase (TRAP) in vitro. The amount of differentiated osteoclastic cells was nearly double in hypertensive mice when compared to the normotensive strain (p < 0.03). Periapical lesion size did not differ between hypertensive and normotensive strains (p > 0.7). IL1α, IL1ß and TNFα cytokines expressions were similar for both systemic conditions (p > 0.05). Despite the fact that no differences could be observed in periapical lesion size and cytokines expressions on the systemic conditions tested, hypertension showed an elevated number of osteoclast differentiation.

Violencia escolar entre adolescentes: condiciones de vulnerabilidad / Violência entre adolescentes escolares: condições de vulnerabilidades / Violence among school adolescents: vulnerability conditionss

La adolescencia es un período de transformación en el que el adolescente se expone a condiciones de vulnerabilidad, así como a situaciones de violencia, tanto en la condición de víctima como en la de agresor. Este estudio tuvo como objetivo identificar el consumo de alcohol, drogas y conflictos familiares, junto con situaciones de violencia, ya sea en forma sufrida, ejercida, o tanto ejercida como sufrida. Estudio transversal realizado en las escuelas públicas en Cuiabá, Mato Grosso, con estudiantes de secundaria. Los datos procesados por Epi-Info se obtuvieron mediante un cuestionario cerrado. Entre 2.786 encuestados, el 44,4% se encontraba en una situación de violencia, en la que el 16,4% eran solo víctimas, 12,0% solamente agresores y 16,0% experimentaron tanto como víctimas como agresoress, predominando la violencia física y el bullying. En cuanto a las condiciones de vulnerabilidad, el 44,1% de los que experimentaron violencia consumen alcohol y 5.5% drogas. En cuanto al alcohol, consumir bebidas destiladas en fiesta, en casa de los amigos, quedando animados cuando beben, con inicio del consumo de 16 a 17 años, se han destacado en todas las situaciones de violencia. En cuanto a las drogas, el consumo en alguna ocasión, empezar a consumir porque un amigo le ofreció, siendo la marihuana la droga de mayor consumo, principalmente porque les gusta y para deshacerse de los problemas, a partir de 16-17 años, se impuso en todas las situaciones de violencia. Los tíos son los miembros de la familia que más consumen alcohol y / o drogas. El estudio pone de relieve la necesidad de acciones integradas entre escuela, servicios de salud, sociedad y familia, como formas de prevenir este fenómeno (AU)

A adolescência é um período de transformações, em que o adolescente está exposto a condições de vulnerabilidades bem como situações de violência, tanto na condição de vítima como na de agressor. Este estudo objetivou identificar o consumo de álcool, drogas e conflito familiar junto às situações de violência, seja na forma sofrida, exercida, e tanto sofrida como exercida. Estudo transversal, desenvolvido em escolas públicas de Cuiabá, Mato Grosso, com adolescentes do ensino médio. Os dados, processados pelo Epi-Info, foram obtidos por meio de questionário fechado. Entre 2.786 pesquisados, 44,4% se encontravam em situação de violência, em que 16,4% eram apenas vítimas, 12,0% somente agressores e 16,0% vivenciaram tanto a condição de vítimas como agressores, predominando a violência física e o bullying. Quanto às condições de vulnerabilidades, 44,1% dos que sofreram violência consomem álcool e 5,5% drogas. Quanto ao álcool, consumir bebidas destiladas, em festa, na casa de amigos, ficando animados quando bebem, com inicio do consumo aos 16-17 anos, se destacaram em todas as situações de violência. Quanto às drogas, consumir de vez em quando, iniciar o consumo porque um amigo ofereceu, sendo a maconha a droga mais consumida, principalmente porque gostam e para se livrar dos problemas, com início aos 16-17 anos, prevaleceram em todas as situações de violência. Os tios foram os membros familiares que mais consomem álcool e/ou drogas. O estudo evidencia a necessidade de ações integradas entre escola, serviços de saúde, sociedade e família, como formas de prevenção deste fenômeno (AU)

Adolescence is a period of transformation, in which the teenager is exposed to conditions of vulnerabilities as well as situations of violence, both as victim and aggressor. This study aimed to identify the consumption of alcohol, drugs and family conflict along with situations of violence, whether in the form suffered, exercised, and both suffered as exercised. A cross-sectional study, developed in public schools in Cuiabá, Mato Grosso, with high school teenagers. The data processed by the Epi-Info, were obtained through a closed questionnaire. Between 2,786 surveyed, 44.4% were in a situation of violence, in which 16.4% were just victims, 12.0% aggressors only, and 16.0% have experienced both the condition of victims as offenders, predominating physical violence and bullying. As regards the conditions of vulnerabilities, 44.1% of those who suffered violence consume alcohol and 5.5% drugs. Regarding alcohol consuming distilled beverages, in party at the home of friends, getting excited when they drink, with beginning of consumption at 16-17 years old, stood out in all situations of violence. Regarding drugs, consume every once in a while, start the consumption because a friend offered, being the most consumed drug marijuana, mainly because they like and to get rid of the problems, beginning at the age of 16-17 years old, prevailed in all situations of violence. The uncles were family members who consume alcohol and/or drugs. The study highlights the need for integrated actions among school, health services, society and family as ways of preventing this phenomenon (AU)

Queimaduras domésticas na população infantojuvenil: atendimentos de urgência e emergência / Domestic burns among children, adolescents and young adults: urgency and emergency cases

Estudo cujo objetivo foi analisar as queimaduras domiciliares decorrentes de exposição à corrente elétrica/radiação/temperatura, fumaça/fogo/chamas e contato com fonte de calor/substâncias quentes, em vítimas crianças, adolescentes e jovens, atendidos em serviço de urgência/emergência de referência. Estudo descritivo, transversal, com vítimas de queimaduras domiciliares (entre zero a 24 anos) atendidas por serviço de pronto-atendimento em uma capital do centro oeste brasileiro, no ano de 2013. Foram estudadas 84 vítimas de queimaduras domiciliares, prevaleceu o sexo feminino (59,5%) e a faixa etária de 1-4 anos (27,4%). As queimaduras mais frequentes foram por contato com fonte de calor/substâncias quentes (82,1%) e exposição à fumaça/fogo/chamas (15,5%). As regiões corpóreas mais atingidas foram cabeça, tronco, membros superiores e inferiores (90,5%) e prevaleceu a queimadura de segundo grau (40,5%). Queimaduras domiciliares atingem significativamente o público infantojuvenil com destaque para o sexo feminino, apontando para a importância de atividades preventivas e educativas direcionadas para as meninas.

The objective of this study was to analyze domestic burns caused by the exposure to electric current/radiation/temperature, smoke/fire/flames and contact with a source of heat/hot substances, in children, adolescents and young adults treated at an urgency/emergency service of reference. A descriptive, cross-sectional study was conducted with victims (0 to 24 years of age) of home burns treated at an urgency/emergency service of a capital city located in the center-west region of Brazil, in 2013. In total, 84 victims of home burns were studied, with prevalence of female victims (59.5%) and age group of 1-4 years of age (27.4%). The most frequent burns were caused by contact with a source of heat/hot substances (82.1%) and exposure to smoke/fire/flames (15.5%). The body areas most commonly affected by home burns were head, trunk, and upper and lower limbs (90.5%), with prevalence of second-degree burns (40.5%). Home burns significantly affect children, adolescents and young adults, particularly female subjects, highlighting the importance of providing preventive and educational activities to female victims.

Hypertension Undermines Mineralization-inducing Capacity of and Tissue Response to Mineral Trioxide Aggregate Endodontic Cement.

INTRODUCTION: This study evaluated the effect of hypertension on tissue response to and mineralization capacity of white and gray mineral trioxide aggregate (MTA) (Angelus Industry Ontological Products, Londrina, Brazil), an endodontic reparative cement. METHODS: Polyethylene tubes containing gray MTA, white MTA, or intermediate restorative material (positive control) or an empty tube (negative control) were implanted into the dorsal connective tissue of spontaneous hypertensive and Wistar rats (n = 12 each). Six rats in each group were sacrificed after 7 days, and the remainder after 30 days. Tubes with surrounding tissue were removed, and a histologic analysis was performed using hematoxylin-eosin and von Kossa staining and examination by polarized light microscopy. RESULTS: The inflammatory response to all materials was greater in hypertensive compared with normotensive rats (P < .05). Positive von Kossa staining and birefringent structures in polarized light were observed for both gray and white MTA (P > .05), but these were more pronounced in normotensive rats (P < .05). Necrotic areas with positive von Kossa staining were observed for intermediate restorative material. CONCLUSIONS: Hypertension undermines tissue repair and mineralization, which can negatively affect treatment outcome. Nonetheless, mineralization in response to MTA was observed even under hypertensive conditions.

Interrelationship Between Periapical Lesion and Systemic Metabolic Disorders.

BACKGROUND: Periapical periodontitis, also known as periapical lesion, is a common dental disease, along with periodontitis (gum disease). Periapical periodontitis is a chronic inflammatory disease, caused by endodontic infection, and its development is regulated by the host immune/inflammatory response. Metabolic disorders, which are largely dependent on life style such as eating habits, have been interpreted as a "metabolically-triggered" low-grade systemic inflammation and may interact with periapical periodontitis by triggering immune modulation. The host immune system is therefore considered the common fundamental mechanism of both disease conditions. METHOD: We have reviewed >200 articles to discuss the interrelationship between periapical lesions and metabolic disorders including type 2 diabetes mellitus, hypertension, and non-alcoholic fatty liver diseases (NAFLD), and their common pathological background in immunology/osteoimmunology and cytokine biology. RESULTS: An elevated inflammatory state caused by metabolic disorders can impact the clinical outcome of periapical lesions and interfere with wound healing after endodontic treatment. Although additional well-designed clinical studies are needed, periapical lesions appear to affect insulin sensitivity and exacerbate non-alcoholic steatohepatitis. CONCLUSION: Immune regulatory cytokines produced by various cell types, including immune cells and adipose tissue, play an important role in this interrelationship.

Evaluation of photodynamic therapy on fibroblast viability and cytokine production.

BACKGROUND: The aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1ß and IL-6) production by mouse fibroblasts. METHODS: Sixty seconds of pre-irradiation time with curcumin 500mg/L and Led wavelength (λ) 480nm, 72Jcm(2), for 300s was used for PDT. Solutions were diluted in culture medium DMEM (1×10(4) cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6, 24 and 48h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p<0.05) for MTT and Kruskal-Wallis test and Dunn (p<0.05) for ELISA. RESULTS: PDT and saline solution presented low cytotoxic effect similar to the control group (p>0.05) while 5% NaOCl was more cytotoxic than PDT (p<0.05) in all periods of time. All materials similarly expressed IL-1ß and IL-6 regardless to the experimental period (p<0.05). CONCLUSIONS: PDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1ß and IL-6.

Relationship between hypertension and periapical lesion: an in vitro and in vivo study

Abstract The aim of this study was to compare potential aspects of periapical lesion formation in hypertensive and normotensive conditions using hypertensive (BPH/2J) and wild-type control (BPN/3J) mice. The mandibular first molars of both strains had their dental pulp exposed. At day 21 the mice were euthanized and right mandibular molars were used to evaluate the size and phenotype of apical periodontitis by microCT. Proteins were extracted from periapical lesion on the left side and the expressions of IL1α, IL1β and TNFα were analyzed by ELISA. Bone marrow stem cells were isolated from adult mice femurs from 2 strains and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase (TRAP) in vitro. The amount of differentiated osteoclastic cells was nearly double in hypertensive mice when compared to the normotensive strain (p < 0.03). Periapical lesion size did not differ between hypertensive and normotensive strains (p > 0.7). IL1α, IL1β and TNFα cytokines expressions were similar for both systemic conditions (p > 0.05). Despite the fact that no differences could be observed in periapical lesion size and cytokines expressions on the systemic conditions tested, hypertension showed an elevated number of osteoclast differentiation.