The case of successful catheter ablation using only the approach from the upper part of the subject's body, with meandering aorta and implanted IVC filter.

A 79-year-old female had paroxysmal supraventricular tachycardia. However, she was implanted with an inferior vena cava filter and her descending aorta had significant meandering. It was thought that the insertion of the catheters would be difficult from the femoral vessels. Therefore we inserted electrode catheters from the right subclavian vein and internal jugular vein. As a result of an electrophysiology study, we diagnosed atrioventricular reciprocating tachycardia with a left lateral concealed accessory pathway (AP). An ablation catheter was introduced retrogradely through the left brachial artery and it was pushed forward under the mitral valve. Furthermore, it was put into the part where the earliest retrograde atrial deflection was recorded under the right ventricular apex pacing, and we succeeded in ablation of the AP. All catheters were inserted only from the upper part of the person's body. As for catheter operability, electric potential, operation time, and fluoroscopy time, there was no change in the case of either approach from the femoral vessels. Because we did not puncture the inguinal region, the patient was able to return to her ward on foot after the operation. In addition, we were able to perform a radical cure without complications.

Controlled release of FGF-2 using fragmin/protamine microparticles and effect on neovascularization.

Water-insoluble fragmin/protamine microparticles of about 0.5-1 mum in diameter were prepared by simple mixing of low-molecular-weight heparin (fragmin) with protamine. We investigated the capability of these microparticles to immobilize fibroblast growth factor (FGF)-2, to protect FGF-2 against degradation, to enhance FGF-2 activity, and to facilitate controlled release of FGF-2. FGF-2 bound to the fragmin/protamine microparticles with high affinity (Kd = 2.08 x 10(-9) M) and the half-life of FGF-2-activity was prolonged substantially through binding of FGF-2 to the microparticles, by protection of FGF-2 from inactivation by heat and proteolysis. After subcutaneous injection into the back of mice, the fragmin/protamine microparticles underwent biodegradation and disappeared in about 2 weeks. A similar injection of FGF-2-containing microparticles resulted in significant neovascularization and fibrous tissue formation near the injection site after 1 week. These results indicate that controlled release of biologically active FGF-2 occurs through both slow diffusion and biodegradation of the microparticles, with subsequent induction of neovascularization. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009.

Penetration analysis of elements and bioleaching treatment of spent refractory for recycling.

Acidithiobacillus ferrooxidans ATCC23270 and Acidithiobacillus thiooxidans TM-32 were used for bioleaching of spent refractories of aluminium and copper melting furnaces for their recycling. Firstly, penetration of elements into aluminium melting furnace refractory was investigated and it was found that up to 7 cm from surface was contaminated. Comparison on leaching efficiency by the strains ATCC23270 and TM-32 found that the strain ATCC23270 could treat larger amount of the refractories than the strain TM-32 could do. In the experiment of bioleaching of spent refractory aluminium melting furnace by the strain ATCC23270, high leaching efficiency were obtained on Al, Si, and Ca, and extremely low leaching performance was, however, shown on the rest of elements i.e., Na, Mn, and Zn. Under the strain TM-32 use, relatively high leaching performance was recognized on Al, Si, Ca, Na, Mn, and Zn. In the experiment of bioleaching for spent refractory copper melting furnace, almost the same leaching trends were shown on Cu, Zn, Al, and Si under the strains ACTT23270 and TM-32 uses.