Characterisation of inorganic microparticles in pigment cells of human gut associated lymphoid tissue.

Macrophages at the base of human gut associated lymphoid tissue (GALT), become loaded early in life with dark granular pigment that is rich in aluminium, silicon, and titanium. The molecular characteristics, intracellular distribution, and source of this pigment is described. Laser scanning and electron microscopy showed that pigmented macrophages were often closely related to collagen fibres and plasma cells in GALT of both small and large intestine and contained numerous phagolysosomes, previously described as granules, that are rich in electron dense submicron sized particles. Morphological assessment, x ray microanalysis, and image electron energy loss spectroscopy showed three distinct types of microparticle: type I - spheres of titanium dioxide, 100-200 nm diameter, characterised as the synthetic food-additive polymorph anatase; type II - aluminosilicates, < 100-400 nm in length, generally of flaky appearance, often with adsorbed surface iron, and mostly characteristic of the natural clay mineral kaolinite; and type III - mixed environmental silicates without aluminium, 100-700 nm in length and of variable morphology. Thus, this cellular pigment that is partly derived from food additives and partly from the environment is composed of inert inorganic microparticles and loaded into phagolysosomes of macrophages within the GALT of all human subjects. These observations suggest that the pathogenicity of this pigment should be further investigated since, in susceptible individuals, the same intracellular distribution of these three types of submicron particle causes chronic latent granulomatous inflammation.

Blackbody calibration sources of high accuracy for a spaceborne infrared instrument: the Along Track Scanning Radiometer.

We describe the two 140-mm-aperture simulated blackbody sources used for the on-board calibration of the Along Track Scanning Radiometer, a spaceborne thermal infrared instrument for the accurate remote sensing of sea surface temperature, in operation since 1991. The design of these spaceborne sources, which operate at ≈ -10 °C and ≈ +30 °C, allows them to meet their unprecedented accuracy goal, namely a 3σ uncertainty in their brightness temperature of <0.1 K for the whole mission. This performance is empirically demonstrated in the laboratory and in flight by long-term temperature readout tests, temperature uniformity measurements, and direct emissivity measurements.