RESUMEN
The aim of the present study was to evaluate the effect of the hydroxyethyl-methacrylate (HEMA) concentration and solvent content of dental adhesives on cell viability and cytokine (IL-1b, IL-6, IL-10, TNF-α) release by human dental pulp cells (HDPCs). HDPCs were obtained from fresh extracted human third molars. Experimental adhesives were prepared containing different concentrations of HEMA (0%, 10%, and 20%) with and without solvent (ethanol 10%). Cylindrical specimens were immersed on culture medium during 24 h to obtain the extracts. The cells were incubated with extracts (culture medium + components leached from the adhesives) of different adhesives, and cell viability and cytokine release were evaluated after 6 and 24 h of exposure. Adhesives containing HEMA promoted high cell viability reduction after 6 h of exposure; but after 24 h, the results were similar to the ones found among control group cells. These effects on cell viability were prominently increased with the addition of solvent. Although IL-1b release was not affected by exposure to eluates, other cytokines (IL-10, IL-6, TNF-α) were modulated by the different experiment conditions, directly influenced by the HEMA concentration and presence of solvent. Higher HEMA concentrations, combined with the presence of solvent, can promote significant reduction on HDPC viability, increasing the release of anti- and pro-inflammatory mediators.
RESUMEN
Objetivo - O cimento MTA tem sido utilizado em perfurações radiculares e obturações retrógradas, devido suas excelentes propriedades de selamento e biocompatibilidade, entretanto, seu custo é elevado. Desta maneira, a proposta deste estudo foi comparar qualitativamente os elementos químicos contidos no cimento de Portland e MTA-Angelus® (Mineral Trióxido Agregado). Métodos - Cem gramas de cimentos MTA e Portland foram submetidas à difração de raios X, com passo de 0,05°/segundos e leitura de intervalo (2?) entre os ângulos 3 e 65°. Difratogramas foram obtidos pelo programa Eva-msc Application e os elementos químicos identificados utilizando fichas de identificação Joint Commitee on Powder Difractin Standard. Resultados - Os minerais calcita (CaCO3), silicato de cálcio (Ca3SiO5) e periclásio (MgO) foram encontrados em ambas as amostras. Não foi observada a presença da fase sulfato de cálcio e potássio (K2Ca2(SO4)3) na amostra de cimento de Portland. Fase amorfa foi observada em ambas as amostras. Conclusão - Os elementos identificados mostraram semelhança química entre o MTA-Angelus® e o cimento de Portland.
Objective - MTA has been used in root perforation and retrograde obturation, due to excellent sealing properties and biocompatibility. However, it is expensive. This way, the purpose of this study was to compare qualitatively the chemical elements in the MTA-Angelus® (Mineral Trioxide Aggregate) and Portland cement. Methods - One hundred grams of MTA and Portland cement were analyzed by x-ray diffraction setting at 3-65° and continuous scans for the (2?) were run a speed of 0.05°/seconds. The diffractograms were obtained by Eva-msc Application Software and chemical elements were identify using Joint Commitee on Powder Difractin Standard identification. Results - Calcite, calcium silicate and periclase were found in the both samples. It was not found potassium sulfate and calcium sulfate in the Portland cement. Amorphous phase was found in both samples. Conclusion - The identified elements showed similar chemical between MTA-Angelus® and Portland cement.
