Adsorption of copper by naturally and artificially aged polystyrene microplastics and subsequent release in simulated gastrointestinal fluid.

Microplastics, especially aged microplastics can become vectors of metals from environment to organisms with potential negative effects on food chain. However, a few studies focused on the bioavailability of adsorbed metals and most studies related to aged microplastics used artificial method that cannot entirely reflect actual aging processes. In this study, virgin polystyrene was aged by ozone (PS-O3), solar simulator (PS-SS) and lake (PS-lake) to investigate adsorption of Cu by virgin, artificially and naturally aged microplastics and subsequent release in simulated gastrointestinal fluids (SGF). Characterization results show carbonyl was formed in PS-O3 and PS-SS, and the oxidation degree was PS-O3 > PS-SS > PS-lake. However, Cu adsorption capacity followed this order PS-lake (158 µg g-1) > PS-SS (117 µg g-1) > PS-O3 (65 µg g-1) > PS-virgin (0). PS-O3 showed highest Cu adsorption capacity at 0.5 h (71 µg g-1), but it dropped dramatically later (10 µg g-1, 120 h), because PS-O3 could break up and the adsorbed Cu released in solutions subsequently. For PS-lake, precipitation of metallic oxides contributes to the accumulation of Cu. The addition of dissolved organic matter (DOM) could occupy adsorption sites on PS and compete with Cu, but also can attach PS and adsorb Cu due to its rich functional groups. The simultaneous ingestion of microplastics with food suggested that adsorbed Cu is solubilized mostly from aged PS to SGF.

Bioaccessibility of Organic Compounds Associated with Tire Particles Using a Fish In Vitro Digestive Model: Solubilization Kinetics and Effects of Food Coingestion.

Tire and road wear particles (TRWP) account for an important part of the polymer particles released into the environment. There are scientific knowledge gaps as to the potential bioaccessibility of chemicals associated with TRWP to aquatic organisms. This study investigated the solubilization and bioaccessibility of seven of the most widely used tire-associated organic chemicals and four of their degradation products from cryogenically milled tire tread (CMTT) into fish digestive fluids using an in vitro digestion model based on Oncorhynchus mykiss. Our results showed that 0.06-44.1% of the selected compounds were rapidly solubilized into simulated gastric and intestinal fluids within a typical gut transit time for fish (3 h in gastric and 24 h in intestinal fluids). The environmentally realistic scenario of coingestion of CMTT and fish prey was explored using ground Gammarus pulex. Coingestion caused compound-specific changes in solubilization, either increasing or decreasing the compounds' bioaccessibility in simulated gut fluids compared to CMTT alone. Our results emphasize that tire-associated compounds become accessible in a digestive milieu and should be studied further with respect to their bioaccumulation and toxicological effects upon passage of intestinal epithelial cells.

In Vitro Digestion of Tire Particles in a Fish Model (Oncorhynchus mykiss): Solubilization Kinetics of Heavy Metals and Effects of Food Coingestion.

Tire and road wear particles (TRWP) have been shown to represent a large part of anthropogenic particles released into the environment. Nevertheless, the potential ecological risk of TRWP in the different environmental compartments and their potential toxic impacts on terrestrial and aquatic organisms remain largely underinvestigated. Several heavy metals compose TRWP, including Zn, which is used as a catalyst during the vulcanization process of rubber. This study investigated the solubilization potential of metals from cryogenically milled tire tread (CMTT) and TRWP in simulated gastric fluids (SFGASTRIC) and simulated intestinal fluids (SFINTESTINAL) designed to mimic rainbow trout (Oncorhynchus mykiss) gastrointestinal conditions. Our results indicate that the solubilization of heavy metals was greatly enhanced by gastrointestinal fluids compared to that by mineral water. After a 26 h in vitro digestion, 9.6 and 23.0% of total Zn content of CMTT and TRWP, respectively, were solubilized into the simulated gastrointestinal fluids. Coingestion of tire particles (performed with CMTT only) and surrogate prey items (Gammarus pulex) demonstrated that the animal organic matter reduced the amount of bioavailable Zn solubilized from CMTT. Contrastingly, in the coingestion scenario with vegetal organic matter (Lemna minor), high quantities of Zn were solubilized from L. minor and cumulated with Zn solubilized from CMTT.

Adsorption of progesterone onto microplastics and its desorption in simulated gastric and intestinal fluids.

The sorption of hydrophobic organic compounds (HOC) onto microplastics is relatively well reported in the literature, while their desorption remains poorly investigated, especially in biological fluids. The present study investigated the sorption and desorption of progesterone on polyethylene (PE), polypropylene (PP), and polystyrene (PS) microplastics. The sorption experiments showed that the equilibrium was reached in a few hours for all plastics. A sorption efficiency of 357.1 µg g-1 was found for PE and PS, and 322.6 µg g-1 for PP. Sorption experiments indicated that adsorption would certainly happen via surface sorption and a potentially pore-filling mechanism. The desorption was carried out in Simulated Gastric Fluid (SGF) and Simulated Intestinal Fluid (SIF), whose formulations were more complex than similar models reported so far. It has been found that the desorption was higher in SIF as compared to SGF, due to micelle formation in SIF promoting the pollutant solubilization. The sorption of pepsin onto microplastics has also been revealed, suggesting a competition between pollutants and pepsin for sorption sites and a potent reduction in pollutant solubilization. This study indicates that the ingestion of microplastics could be considered as an additional route of exposure to pollutants and therefore emphasizes pollutant bioavailability for aquatic organisms.

PCB mass budget in a perialpine lake undergoing natural decontamination in a context of global change.

Despite the fact that PCB contamination of the global environment has been extensively studied in the last decades, the fate of these compounds in freshwater ecosystems is not fully understood and an important knowledge gap remains regarding the understanding of PCB dynamics and fate in perialpine lakes. This study relied on both field sampling performed and modeling to accurately identify the main fluxes involved in the PCB dynamics into the French perialpine Lake Bourget from 2013 to 2017. Our results show that the main inputs responsible for the PCB loading of the water column are tributaries inflows (~90%) rather than atmospheric inputs which could be related to the high catchment area over lake surface area ratio (i.e., 13). The main mechanism responsible for the lake natural decontamination was sediment burial (76%) due to the effect of the biological pump coupled with a high sedimentation rate. Volatilization represented 19% of the loss of PCBs from the water column and was mainly controlled by the high PCB concentration in water. These mechanisms are susceptible to be affected by the impact of the global change (increase of temperature, modification of the primary production rate) in the near future.