A novel diagnostic approach to Paratuberculosis in dairy cattle using near-infrared spectroscopy and aquaphotomics.

Introduction: As a contagious and chronic disease in the livestock industry, Paratuberculosis is a significant threat to dairy herds' genetic and economic resources. Due to intensive breeding and high production of dairy cattle, the incidence and prevalence are higher. Developing non-destructive diagnostic methods for the early detection and identification of healthy animals is paramount for breeding programs. Conventional methods are almost entirely destructive, have low accuracy, lack precision, and are time-consuming. Near-infrared spectroscopy (NIRS) and aquaphotomics can detect changes in biofluids and thus have the potential to diagnose disease. This study aimed to investigate the diagnostic ability of NIRS and aquaphotomics for Paratuberculosis in dairy cattle. Methods: Blood plasma from dairy cattle was collected in the NIR range (1,300 nm to 1,600 nm) 60 days before and 100 days to 200 days after calving in two groups, positive and negative, using the same consecutive enzyme-linked immunosorbent assay test results three times as a reference test. Results: NIRS and aquaphotomics methods invite 100% accuracy, sensitivity, and specificity to detect Paratuberculosis using data mining by unsupervised method, Principal Component Analysis, and supervised methods: Soft Independent Modeling of Class Analogiest, Linear Discriminant Analysis, Quadratic Discriminant Analysis, Partial Least Square-Discriminant Analysis, and Support Vector Machine models. Discussion: The current study found that monitoring blood plasma with NIR spectra provides an opportunity to analyze antibody levels indirectly via changes in water spectral patterns caused by complex physiological changes, such as the amount of antibodies related to Paratuberculosis by aquagram.

An Analytical Quantum Model to Calculate Fluorescence Enhancement of a Molecule in Vicinity of a Sub-10 nm Metal Nanoparticle.

An analytical quantum model is used to calculate electrical permittivity of a metal nanoparticle located in an adjacent molecule. Different parameters, such as radiative and non-radiative decay rates, quantum yield, electrical field enhancement factor, and fluorescence enhancement are calculated by such a model and they are compared with those obtained by using the classical Drude model. It is observed that using an analytical quantum model presents a higher enhancement factor, up to 30%, as compared to classical model for nanoparticles smaller than 10 nm. Furthermore, the results are in better agreement with those experimentally realized.

Nonlinear optical microscopy improvement by focal-point axial modulation.

Among the most important challenges of microscopy­even more important than the resolution enhancement, especially in biological and neuroscience applications­is noninvasive and label-free imaging deeper into live scattering samples. However, the fundamental limitation on imaging depth is the signal-to-background ratio in scattering biological tissues. Here, using a vibrating microscope objective in conjunction with a lock-in amplifier, we demonstrate the background cancellation in imaging the samples surrounded by turbid and scattering media, which leads to more clear images deeper into the samples. Furthermore, this technique offers the localization and resolution enhancement as well as resolves ambiguities in signal interpretation, using a single-color laser. This technique is applicable to most nonlinear as well as some linear point-scanning optical microscopies.

Comparison of clinical bracket point registration with 3D laser scanner and coordinate measuring machine.

OBJECTIVE: The aim of the present study was to assess the diagnostic value of a laser scanner developed to determine the coordinates of clinical bracket points and to compare with the results of a coordinate measuring machine (CMM). METHODS: This diagnostic experimental study was conducted on maxillary and mandibular orthodontic study casts of 18 adults with normal Class I occlusion. First, the coordinates of the bracket points were measured on all casts by a CMM. Then, the three-dimensional coordinates (X, Y, Z) of the bracket points were measured on the same casts by a 3D laser scanner designed at Shahid Beheshti University, Tehran, Iran. The validity and reliability of each system were assessed by means of intraclass correlation coefficient (ICC) and Dahlberg's formula. RESULTS: The difference between the mean dimension and the actual value for the CMM was 0.0066 mm. (95% CI: 69.98340, 69.99140). The mean difference for the laser scanner was 0.107 ± 0.133 mm (95% CI: -0.002, 0.24). In each method, differences were not significant. The ICC comparing the two methods was 0.998 for the X coordinate, and 0.996 for the Y coordinate; the mean difference for coordinates recorded in the entire arch and for each tooth was 0.616 mm. CONCLUSION: The accuracy of clinical bracket point coordinates measured by the laser scanner was equal to that of CMM. The mean difference in measurements was within the range of operator errors.

Comparison of clinical bracket point registration with 3D laser scanner and coordinate measuring machine

OBJECTIVE: The aim of the present study was to assess the diagnostic value of a laser scanner developed to determine the coordinates of clinical bracket points and to compare with the results of a coordinate measuring machine (CMM). METHODS: This diagnostic experimental study was conducted on maxillary and mandibular orthodontic study casts of 18 adults with normal Class I occlusion. First, the coordinates of the bracket points were measured on all casts by a CMM. Then, the three-dimensional coordinates (X, Y, Z) of the bracket points were measured on the same casts by a 3D laser scanner designed at Shahid Beheshti University, Tehran, Iran. The validity and reliability of each system were assessed by means of intraclass correlation coefficient (ICC) and Dahlberg's formula. RESULTS: The difference between the mean dimension and the actual value for the CMM was 0.0066 mm. (95% CI: 69.98340, 69.99140). The mean difference for the laser scanner was 0.107 ± 0.133 mm (95% CI: -0.002, 0.24). In each method, differences were not significant. The ICC comparing the two methods was 0.998 for the X coordinate, and 0.996 for the Y coordinate; the mean difference for coordinates recorded in the entire arch and for each tooth was 0.616 mm. CONCLUSION: The accuracy of clinical bracket point coordinates measured by the laser scanner was equal to that of CMM. The mean difference in measurements was within the range of operator errors. .

OBJETIVO: o objetivo do presente estudo foi avaliar o valor diagnóstico de um scanner a laser desenvolvido para determinar as coordenadas dos pontos de colagem de braquetes, comparando seus resultados aos resultados obtidos com uma máquina de medição coordenada (MMC). MÉTODOS: esse estudo experimental diagnóstico foi conduzido com modelos ortodônticos obtidos a partir da arcada superior de 18 pacientes adultos, com oclusão normal de Classe I. Inicialmente, as coordenadas dos pontos de colagem de braquetes de todos os modelos foram mensuradas por uma MMC. Em seguida, as coordenadas tridimensionais (X, Y, Z) dos pontos foram mensuradas nos mesmos modelos por um scanner a laser 3D, desenvolvido na Universidade de Shahid Beheshti. A eficácia e confiabilidade dos dois sistemas foram avaliadas pelo Coeficiente de Correlação Intraclasse (CCI) e pela fórmula de Dahlberg. RESULTADOS: a diferença entre a média da dimensão mensurada pela MMC e o valor real obtido foi de 0,0066mm (IC 95%: 69,98340 - 69,99140). A diferença média para o scanner a laser foi de 0,107 ± 0,133 (95% IC: -0,002 - 0,24). Em cada método, as diferenças não foram significativas. Ao comparar os dois métodos, o CCI gerou um valor de 0,998 para a coordenada X e de 0,996 para a coordenada Y. A diferença média para as coordenadas registradas em cada dente da arcada foi de 0,616mm. CONCLUSÃO: a precisão das coordenadas do ponto de colagem dos braquetes foi a mesma no scanner a laser e na MMC. A diferença média entre as medições manteve-se dentro dos limites de erros operacionais. .

Validity and reliability of a three-dimensional dental cast simulator for arch dimension measurements.

BACKGROUND: The accuracy and reproducibility of measurements in a locally made three dimensional (3D) simulator was assessed and compared with manual caliper measurements. MATERIALS AND METHODS: A total of 20 casts were scanned by our laser scanner. Software capabilities included dimensional measurements, transformation and rotation of the cast as a whole, separation and rotation of each tooth and clip far. Two orthodontists measured the intercanine width, intermolar width and canine, molar and arch depth on the casts and in 3D simulator. For calculating the reliability coefficient and comparing random and systematic errors between the two methods, intra-class correlation coefficient of reliability (ICC), Dahlberg and paired t-test were used, respectively. The ICC and Dahlberg's formula were also applied to assess intra-examiner and inter-examiner reliability of measurements on the casts and in the simulator (P < 0.05). RESULTS: Canine and molar depth measurements had low reliability on the casts. Reliability between methods for the remaining three variables was 0.87, 0.98 and 0.98 in the maxilla and 0.92, 0.77 and 0.94 in the mandible, respectively. The method error was between 0.31 and 0.48 mm. The mean intra-observer difference were 0.086 and 0.23 mm in the 3D method and caliper. The inter-observer differences were 0.21 and 0.42 mm, respectively. CONCLUSION: The maximum average absolute difference between the two methods was <0.5 mm, indicating that the new system is indeed clinically acceptable. The examiner reliability was higher in 3D measurements.

Modeling of femtosecond pulse propagation inside x-cut and z-cut MgO doped LiNbO3 anisotropic crystals.

A four-dimensional spatiotemporal nonlinear Schrödinger equation coupled with a plasma equation is solved for anisotropic crystal of MgO doped lithium niobate. The modeling is performed for x-cut and z-cut lithium niobate crystals commonly used for waveguide writing by femtosecond lasers. The effect of various parameters such as energy, duration, and polarization of the incident laser pulse on distribution of the plasma density in the vicinity of focus is studied. Our simulations reveal that the maximum plasma density in the vicinity of the focus is higher for longer pulse durations and higher pulse energies. Furthermore, it is observed that for the same peak powers of the incident pulse, the plasma density generated inside the crystal is higher for linear polarization as compared to circular polarization.

Spatially resolved laser-induced breakdown spectroscopy in methane-air diffusion flames.

A new setup for spatially resolved laser-induced breakdown spectroscopy (SR-LIBS) is used for the first time to analyze methane-air diffusion flames. Using this configuration, background continuum emission is reduced, signal-to-background noise ratio is increased up to eight times, and spatial resolution is enhanced. The local equivalence ratio is also quantitatively estimated and the width of the secondary combustion region at a specified height above the burner is determined for two different methane flow rates. Furthermore, the threshold energy for spark formation is measured for regions inside and outside the flame. The results show that threshold energy is larger at the secondary combustion region, near the border of the flame, than inside the flame.

Study of short-pulse laser propagation in biological tissue by means of the boundary element method.

Propagation of short pulses of light through biological tissues can be studied by numerically solving the diffusion equation. The boundary integral method was used to convert the differential equation to integral form and the result was solved using the boundary element method. The effects of different optical parameters of the tissue, i.e. scattering, absorption coefficients and anisotropic factor, on temporal evolution of the diffusely reflected pulse were studied. The results were compared with those obtained using the finite difference time domain method and the boundary integral method was found to be more precise and faster than the last method. The method can be used to investigate reflected pulses in the study of cell morphology and tumours in different types of tissue.

Study on scattering coefficient of surface plasmon polariton waves at interface of two metal-dielectric waveguides by using G-GFSIEM method.

Generalized Green's Function Surface Integral Equation Method (G-GFSIEM) is used to study propagation of surface plasmon polariton waves at interface of two semi-infinite metal-dielectric waveguides. Reflection, transmission, and scattering coefficients for structures with different dielectric constants are calculated by using this method and by using energy conservation law. Conditions where scattering coefficient is maximized or minimized are studied. It is found that by using appropriate materials with specified dielectric constants, structures with required reflection, transmission, and scattering coefficients can be designed.

Boundary integral method for simulating laser short-pulse penetration into biological tissues.

The study of short-pulse propagation through biological tissues is important due to the medical applications of short-pulse lasers. Techniques used for numerical study of short pulses through human tissues include the Monte Carlo (MC) method, the finite-element method, and the finite-difference time-domain (FDTD), but these are often time consuming. Recently, the boundary integral method (BIM) was applied to overcome this problem. The literature shows that the BIM is faster than the other mentioned methods. We first investigate the precision of results obtained by the BIM by comparison with those results obtained by the MC and FDTD methods. Then we use the BIM to investigate the short-pulse penetration into biological tissues. We also study the effects of optical properties of tissues such as scattering, the absorption coefficient, the anisotropic factor on the penetrating pulse. We also, consider the propagation of pulses emitted from extended sources with different temporal evolutions.

The accuracy of a 3-D laser scanner for crown width measurements.

BACKGROUND: Virtual dental casts have been recently introduced to orthodontics. The problem of capturing the shapes of teeth on study casts may be complicated by the presence of undercut areas and deep grooves. AIMS: This study aimed to develop a 3-D laser scanner and associated software, and to evaluate the reproducibility and validity of mesiodistal crown width measurements based on slice distinction. METHODS: To evaluate reproducibility: a cube was scanned with readily available equipment and the digital measurements compared to caliper measurements of the cube; and the mesiodistal widths of artificial upper teeth were measured, set-up in a dental model, scanned, measured on the virtual study models and compared with the reference measurements. Custom software was used to capture and process the resulting images. To determine validity artificial teeth were measured with calipers, set-up in 20 different malocclusions, duplicated and scanned. The caliper measurements of groups of teeth (incisors, canines, premolars, molars) were compared with digital measurements of the same teeth. In the second method to determine validity, 10 dental casts were scanned and measured by two examiners and the mesio-distal widths compared with the reference values, and with each other. RESULTS: The digital measurements of the cube fell within 0.1 mm of the reference value. The absolute error in repeated measures of the dental model was 0.32 +/- 0.25 mm. The overall correlation between 3-D images of the teeth and the reference values, using the intraclass correlation coefficient (ICC), was 0.84. The most and least valid results for groups of teeth were the premolars and canines, respectively. The inter-observer ICC was 0.60, and each examiner's ICC versus the reference values were 0.833 and 0.855. CONCLUSIONS: The mesiodistal widths of teeth and groups of teeth can be reliably and validly measured on virtual study models using the scanner and software developed for this purpose. Inaccuracy in the canine region may be overcome by using a smaller rotational angle during scanning.