RESUMEN
The dynamic evolution of photogrammetry led to the development of numerous methods of geometric calibration of cameras, which are mostly based on building flat targets (fields) with features that can be distinguished in the images. Geometric calibration of thermal cameras for UAVs is an active research field that attracts numerous researchers. As a result of their low price and general availability, non-metric cameras are being increasingly used for measurement purposes. Apart from resolution, non-metric sensors do not have any other known parameters. The commonly applied process is self-calibration, which enables the determining of the approximate elements of the camera's interior orientation. The purpose of this work was to analyze the possibilities of geometric calibration of thermal UAV cameras using proposed test field patterns and materials. The experiment was conducted on a FLIR VUE PRO thermal camera dedicated to UAV platforms. The authors propose the selection of various image processing methods (histogram equalization, thresholding, brightness correction) in order to improve the quality of the thermograms. The consecutive processing methods resulted in over 80% effectiveness on average by 94%, 81%, and 80 %, respectively. This effectiveness, for no processing and processing with the use of the filtering method, was: 42% and 38%, respectively. Only high-pass filtering did not improve the obtained results. The final results of the proposed method and structure of test fields were verified on chosen geometric calibration algorithms. The results of fast and low-cost calibration are satisfactory, especially in terms of the automation of this process. For geometric correction, the standard deviations for the results of specific methods of thermogram sharpness enhancement are two to three times better than results without any correction.
RESUMEN
The impact of planktonic and biofilm lifestyles of the clinical isolate Proteus mirabilis 9B-m on its lipopolysaccharide (O-polysaccharide, core region, and lipid A) was evaluated. Proteus mirabilis bacteria are able to form biofilm and lipopolysaccharide is one of the factors involved in the biofilm formation. Lipopolysaccharide was isolated from planktonic and biofilm cells of the investigated strain and analyzed by SDS-PAGE with silver staining, Western blotting and ELISA, as well as NMR and matrix-assisted laser desorption ionization time-of-flight mass spectrometry techniques. Chemical and NMR spectroscopic analyses revealed that the structure of the O-polysaccharide of P. mirabilis 9B-m strain did not depend on the form of cell growth, but the full-length chains of the O-antigen were reduced when bacteria grew in biofilm. The study also revealed structural modifications of the core region in the lipopolysaccharide of biofilm-associated cells-peaks assigned to compounds absent in cells from the planktonic culture and not previously detected in any of the known Proteus core oligosaccharides. No differences in the lipid A structure were observed. In summary, our study demonstrated for the first time that changes in the lifestyle of P. mirabilis bacteria leads to the modifications of their important virulence factor-lipopolysaccharide.