RESUMEN
Background: Chronic periodontitis is the most common dental disease reported globally as well as in India. Periodontal pathogens are usually seen in samples of gingival tissues, crevicular blood, GCF (gingival crevicular fluid), and dental plaque. Apart from the conventional mechanical treatment, laser disinfection is a recent advancement that change shows greater efficacy in reducing the disease progression and changing the bacterial flora. Aim: The present study aimed to assess the Immediate response of diode laser on the microbial load in subjects with chronic Periodontitis as assessed in saliva, crevicular blood, and GCF (gingival crevicular fluid) samples. Materials and Methods: The study recruited 90 subjects with chronic periodontitis. For split-mouth fashion, the mouth, of each participant was divided into two halves and was divided into two groups randomly. Group I (test group) subjects underwent laser disinfection (970 ± 15 nm). Group II subjects served as controls and underwent saline irrigation. For all participants, crevicular blood, saliva, and GCF samples were collected before and immediately following disinfection for microbial analysis. Results: Microbial load reduction was seen in both groups following treatment. However, a significantly higher reduction was seen in the test group with laser disinfection. Compared to the crevicular blood sample, a greater reduction was seen in saliva and GCF samples. Conclusion: The present study concludes that Diode Laser (970 ± 15 nm) application shows an immediate reduction of the bacterial load in subjects with chronic periodontitis.
RESUMEN
Background: Dental implants are considered better, latest, and most advanced technique of teeth replacement in present times with more teeth loss and increased related concerns. Aims: The present clinical trial was carried out to assess marginal bone loss and implant failure in immediate and delayed loading implants. The study also evaluated healing using Polymerase Chain Reaction (PCR) and the effect of risk factors on marginal bone loss. Materials and Methods: The 44 subjects were randomly divided into two groups with immediate loading and delayed loading protocols. Various soft-tissue parameters were seen clinically. Quantitative PCR was done to detect biomarkers. The collected data were subjected to statistical evaluation with a level of significance at P < 0.05 and the results were formulated. Results: Concerning marginal bone loss, it was seen that for delayed loading, the bone loss at the implant level was 1.52 ± 0.14, 0.19 ± 0.11, and 0.40 ± 0.12, respectively, at placement, 1 and 2 years. Plaque and mucosal bleeding scores were low at the time of placement with respective values of 0.96 ± 0.12 and 28.42 ± 3.15 for the delayed loading group and 0.98 ± 0.11 and 30.24 ± 3.15 for the immediate loading group. Tartrate-resistant acid phosphatase (TRAP) showing remodeling was high at 3 months in delayed loading (13.3 ± 8.5). Alkaline Phosphatase (ALP) was highest in delayed loading at 3 months (25.2 ± 7.7) and immediate loading at 2 days (32.6 ± 13). Conclusion: Both immediate loading and delayed loading implants show similar results in terms of bone loss, soft-tissue parameters, and biomarkers in sulcular fluids with relatively few and manageable complications.