RESUMEN
Since the publication of the modified Prospective Investigation of Pulmonary Embolism Diagnosis (PIOPED) criteria for the diagnosis of pulmonary embolism (PE), new clinical and scintigraphic diagnostic algorithms (the McMaster clinical criteria, the PisaPED simplified scintigraphic grading and the Miettinen logistic regression analysis) have been reported although the results have not been reproduced in other sites. Ventilation-perfusion lung scintigraphy was performed in 238 consecutive patients with a provisional diagnosis of PE. Scans were reported as normal/very low, low, intermediate or high probability for PE using standardized criteria. Each patient received a clinical grading of probability of PE as low, moderate or high using the McMaster clinical criteria. Using the PisaPED criteria (an alternate simplified scintigraphic grading system using the perfusion scan alone) each scan was also graded as normal/near normal, abnormal but not PE, or abnormal and PE. Using the logistic regression algorithm of Miettinen each scan received a numerical probability of PE. Frequencies for differing levels of probability of PE varied widely between the various algorithms. Cross tabulations revealed correlation of the standardized criteria with the Miettinen grading but not with the McMaster or the PisaPED gradings. We were unable to reproduce similar results using the McMaster clinical grading or the PisaPED simplified scintigraphic grading although the Miettinen logistic regression formula gave comparable results. New algorithms are not automatically transferable to new environments.
Asunto(s)
Algoritmos , Embolia Pulmonar/diagnóstico por imagen , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Modelos Logísticos , Masculino , Persona de Mediana Edad , Embolia Pulmonar/diagnóstico , Cintigrafía , Reproducibilidad de los Resultados , Índice de Severidad de la Enfermedad , Pertecnetato de Sodio Tc 99m , Agregado de Albúmina Marcado con Tecnecio Tc 99mRESUMEN
WN virus is one of the most ubiquitous arboviruses occurring over a broad geographical range and in a wide diversity of vertebrate host and vector species. The virus appears to be maintained in endemic foci on the African continent and is transported annually to temperate climates to the north in Europe and to the south in South Africa. Reports of clinical disease due to natural WN virus infection in wild or domestic animals were much less common than reports of infection (virus isolation or antibody detection). Until recently, records of morbidity and mortality in wild birds were confined to a small number of cases and infections causing encephalitis, sometimes fatal, in horses were reported infrequently. In the period 1996-2001, there was an increase in outbreaks of illness due to WN virus in animals as well as humans. Within the traditional range of WN virus, encephalitis was reported in horses in Italy in 1998 and in France in 2000. The first report of disease and deaths caused by WN virus infection in domestic birds was reported in Israel in 1997-1999, involving hundreds of young geese. In 1999 WN virus reached North America and caused an outbreak of encephalitis in humans in the New York area at the same time as a number of cases of equine encephalitis and deaths in American crows and a variety of other bird species, both North American natives and exotics. Multi-state surveillance for WN virus has been in place since April 2000 and has resulted in the detection of WN virus in thousands of dead birds from an increasing number of species in North America, and also in several species of mammals. The surveillance system that has developed in North America because of the utility of testing dead birds for the rapid detection of WN virus presence has been a unique integration of public health and wildlife health agencies. It has been suggested that the recent upsurge in clinical WN virus infection in wild and domestic animals as well as in humans may be related to the emergence of one or more new strains of WN virus. Virus isolated in New York in 1999 was found to be identical to that from Israel. It was alarming for WN virus to so easily invade the United States and surprising that it became established so quickly in the temperature climate of New York. Its persistence and rapid expansion in the United States leave a number of unanswered questions. New disease characteristics and patterns have occurred and more are evolving as WN virus further invades the western hemisphere. Additional animal research is needed to answer these questions. Some of the research needs include bird migration as a mechanism of virus dispersal, vector and vertebrate host relationships, virus persistence mechanisms, laboratory diagnosis, viral pathogenesis, risk factor studies, vaccine development, and WN virus impact on wildlife (CDC 2001a). Determination of the primary reservoir host species that are involved in the epidemiology of WN virus and the suitable sentinel species for active surveillance are also important research areas.
Asunto(s)
Animales Domésticos/virología , Animales Salvajes/virología , Fiebre del Nilo Occidental/veterinaria , Virus del Nilo Occidental/aislamiento & purificación , Virus del Nilo Occidental/patogenicidad , Anfibios/virología , Animales , Aves/virología , Brotes de Enfermedades/veterinaria , Reservorios de Enfermedades , Ecosistema , Enfermedades de los Caballos/etiología , Caballos/virología , Humanos , Control de Insectos , Mamíferos/virología , Vigilancia de la Población , Reptiles/virología , Fiebre del Nilo Occidental/etiologíaRESUMEN
The Brucella abortus vaccine strain RB51 (SRB51) is being considered for use in the management of bnucellosis in wild bison (Bison bison) and elk (Cervus elaphus) populations in the Greater Yellowstone Area (USA). Evaluation of the vaccines safety in non-target species was considered necessary prior to field use. Between June 1998 and December 1999, ground squirrels (Spermophilus richardsonii, n = 21), deer mice (Peromyscus maniculatus, n = 14), prairie voles (Microtus ochrogaster, n = 21), and ravens (Corvus corax, n = 13) were orally inoculated with SRB51 or physiologic saline. Oral and rectal swabs and blood samples were collected for bacteriologic evaluation. Rodents were necropsied at 8 to 10 wk and 12 to 21 wk post inoculation (PI), and ravens at 7 and 11 wk PI. Spleen, liver and reproductive tissues were collected for bacteriologic and histopathologic evaluation. No differences in clinical signs, appetite, weight loss or gain, or activity were observed between saline- and SRB51-inoculated animals in all four species. Oral and rectal swabs from all species were negative throughout the study. In tissues obtained from SRB51-inoculated animals, the organism was isolated from six of seven (86%) ground squirrels, one of six (17%) deer mice, none of seven voles, and one of five (20%) ravens necropsied at 8, 8, 10, and 7 wk PI, respectively. Tissues from four of seven (57%) SRB51-inoculated ground squirrels were culture positive for the organism 12 wk PI; SRB51 was not recovered from deer mice, voles. or ravens necropsied 12, 21, or 11 wk, respectively, PI. SRB51 was not recovered from saline-inoculated ground squirrels, deer mice, or voles at any time but was recovered from one saline-inoculated raven at necropsy, 7 wk PI, likely attributable to contact with SRB51-inoculated ravens in an adjacent aviary room. Spleen was time primary tissue site of colonization in ground squirrels, followed by the liver and reproductive organs. The results indicate oral exposure to SRB51 does not produce morbidity or mortality in ravens, ground squirrels, deer mice, or prairie voles.
Asunto(s)
Enfermedades de las Aves/prevención & control , Vacuna contra la Brucelosis/administración & dosificación , Brucella abortus/inmunología , Brucelosis/veterinaria , Enfermedades de los Roedores/prevención & control , Animales , Arvicolinae , Enfermedades de las Aves/etiología , Vacuna contra la Brucelosis/efectos adversos , Brucella abortus/aislamiento & purificación , Brucelosis/etiología , Brucelosis/prevención & control , Recuento de Colonia Microbiana , Femenino , Hígado/microbiología , Hígado/patología , Masculino , Peromyscus , Enfermedades de los Roedores/etiología , Seguridad , Sciuridae , Pájaros Cantores , Bazo/microbiología , Bazo/patología , Resultado del TratamientoRESUMEN
The ecology of the strain of West Nile virus (WNV) introduced into the United States in 1999 has similarities to the native flavivirus, St. Louis encephalitis (SLE) virus, but has unique features not observed with SLE virus or with WNV in the old world. The primary route of transmission for most of the arboviruses in North America is by mosquito, and infected native birds usually do not suffer morbidity or mortality. An exception to this pattern is eastern equine encephalitis virus, which has an alternate direct route of transmission among nonnative birds, and some mortality of native bird species occurs. The strain of WNV circulating in the northeastern United States is unique in that it causes significant mortality in exotic and native bird species, especially in the American crow (Corvus brachyrhynchos). Because of the lack of information on the susceptibility and pathogenesis of WNV for this species, experimental studies were conducted at the USGS National Wildlife Health Center. In two separate studies, crows were inoculated with a 1999 New York strain of WNV, and all experimentally infected crows died. In one of the studies, control crows in regular contact with experimentally inoculated crows in the same room but not inoculated with WNV succumbed to infection. The direct transmission between crows was most likely by the oral route. Inoculated crows were viremic before death, and high titers of virus were isolated from a variety of tissues. The significance of the experimental direct transmission among captive crows is unknown.
Asunto(s)
Enfermedades de las Aves/epidemiología , Enfermedades de las Aves/transmisión , Pájaros Cantores , Fiebre del Nilo Occidental/veterinaria , Virus del Nilo Occidental/patogenicidad , Animales , Culex , Humanos , Estados Unidos/epidemiología , Fiebre del Nilo Occidental/epidemiología , Fiebre del Nilo Occidental/transmisiónRESUMEN
A serologic survey of swift fox (Vulpes velox) and kit fox (V. macrotis) from the western USA was conducted for 12 infectious diseases. Samples from swift fox were collected between 1987 and 1992 from Colorado (n = 44), Kansas (n = 10), and Wyoming (n = 9). Samples from kit fox were collected in California (n = 86), New Mexico (n = 18), Utah (n = 9), and Arizona (n = 6). Overall antibody prevalence rates were 33 of 110 (30%) for canine parvovirus (CPV), 9 of 72 (13%) for canine distemper virus (CDV), 23 of 117 (20%) for vesicular stomatitis New Jersey, 16 of 117 (14%) for vesicular stomatitis Indiana, six of 117 (5%) for Cache Valley virus, five of 117 (4%) for Jamestown Canyon virus, one of 97 (1%) for rabies virus, one of 117 (1%) for Colorado tick fever virus, and one of 117 (1%) for western equine encephalitis virus. In addition, antibodies were not found to Yersinia pestis, Francisella tularensis, and Borrelia burgdorferi in serum from 25 Colorado swift fox. Adult swift fox from Colorado had serologic evidence of exposure to CPV more often than juveniles. No juvenile swift fox from Colorado had serum antibodies to CDV. There were season-specific differences in serum antibody prevalence for CPV for swift fox from Colorado. No viruses were isolated from ectoparasites or fox from Colorado.
Asunto(s)
Conservación de los Recursos Naturales , Zorros/virología , Vesiculovirus , Virosis/veterinaria , Animales , Anticuerpos Antivirales/sangre , Colorado/epidemiología , Fiebre por Garrapatas del Colorado/epidemiología , Fiebre por Garrapatas del Colorado/veterinaria , Virus de la Fiebre por Garrapatas del Colorado/inmunología , Moquillo/epidemiología , Virus del Moquillo Canino/inmunología , Virus de la Encefalitis Equina del Oeste/inmunología , Infecciones por Parvoviridae/epidemiología , Infecciones por Parvoviridae/veterinaria , Parvovirus Canino/inmunología , Virus de la Rabia/aislamiento & purificación , Estudios Seroepidemiológicos , Virus de la Estomatitis Vesicular Indiana/inmunología , Virosis/epidemiologíaRESUMEN
We performed a retrospective utilization study covering a four-year period to determine how physicians familiar with colon transit scintigraphy (CTS) use it to manage patients with chronic constipation. Following CTS, there was a change is both frequency and likelihood of diagnosis. The diagnosis was changed in 51% of patients-37% of those considered to have slow transit constipation (STC) before CTS, 43% with obstructed defecation, and 64% with functional bowel disease (FBD). CTS increased the diagnostic likelihood in all groups. Of patients with the diagnosis of STC, 16% were considered "almost certain" before CTS while 83% were considered "almost certain" after CTS. For FBD comparable percentages were 13% and 62%. CTS may play a major role in the diagnostic work-up of patients with chronic constipation, both altering diagnosis and increasing diagnostic certainty.
Asunto(s)
Algoritmos , Estreñimiento/diagnóstico por imagen , Tránsito Gastrointestinal/fisiología , Adolescente , Adulto , Anciano , Enfermedad Crónica , Estreñimiento/fisiopatología , Femenino , Humanos , Masculino , Métodos , Persona de Mediana Edad , Cintigrafía , Estudios Retrospectivos , Encuestas y CuestionariosRESUMEN
BACKGROUND: No randomized controlled trial of prostate cancer screening has been reported and none is likely to be completed in the near future. In the absence of direct evidence, the decision to screen must therefore be based on estimates of benefits and risks. The main risk of screening is overdetection--the detection of cancer that, if left untreated, would not cause death. In this study the authors estimate the level of overdetection that might result from annual screening of men aged 50-70. METHODS: The annual rate of lethal screen-detectable cancer (detectable cancer that would prove fatal before age 85 if left untreated) was calculated from the observed prostate cancer mortality rate in Quebec; the annual rate of all cases of screen-detectable prostate cancer was calculated from 2 recent screening studies. RESULTS: The annual rate of lethal screen-detectable prostate cancer was estimated to be 1.3 per 1000 men. The annual rate of all cases of screen-detectable prostate cancer was estimated to be 8.0 per 1000 men. The estimated case-fatality rate among men up to 85 years of age was 16% (1.3/8.0) (sensitivity analysis 13% to 22%). INTERPRETATION: Of every 100 men with screen-detected prostate cancer, only 16 on average (13 to 22) could have their lives extended by surgery, since the prostate cancer would not cause death before age 85 in the remaining 84 (78 to 87).
Asunto(s)
Tamizaje Masivo/efectos adversos , Tamizaje Masivo/normas , Selección de Paciente , Neoplasias de la Próstata/diagnóstico , Anciano , Anciano de 80 o más Años , Sesgo , Biopsia , Causas de Muerte , Femenino , Humanos , Masculino , Tamizaje Masivo/métodos , Persona de Mediana Edad , Antígeno Prostático Específico/sangre , Neoplasias de la Próstata/sangre , Neoplasias de la Próstata/mortalidad , Quebec/epidemiología , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
In October 1995, epidemic "hemorrhagic fever," without jaundice or renal manifestations, was reported in rural Nicaragua following heavy flooding; 2259 residents were evaluated for nonmalarial febrile illnesses (cumulative incidence, 6.1%) and 15 (0.7%) died with pulmonary hemorrhage. A case-control study found that case-patients were more likely than controls to have ever walked in creeks (matched odds ratio [MOR], 15.0; 95% confidence interval [CI], 1.7-132.3), have household rodents (MOR, 10.4; 95% CI, 1.1-97.1), or own dogs with titers >/=400 to Leptospira species (MOR, 23.4; 95% CI, 3.6-infinity). Twenty-six of 51 case-patients had serologic or postmortem evidence of acute leptospirosis. Leptospira species were isolated from case-patients and potential animal reservoirs. This leptospirosis epidemic likely resulted from exposure to flood waters contaminated by urine from infected animals, particularly dogs. Leptospirosis should be included in the differential diagnosis for nonmalarial febrile illness, particularly during periods of flooding or when pulmonary hemorrhage occurs.
Asunto(s)
Hemorragia/complicaciones , Leptospirosis/epidemiología , Enfermedades Pulmonares/complicaciones , Adolescente , Adulto , Animales , Estudios de Casos y Controles , Bovinos , Niño , Preescolar , Desastres , Brotes de Enfermedades , Vectores de Enfermedades , Perros , Hemorragia/microbiología , Caballos , Humanos , Incidencia , Lactante , Leptospira/clasificación , Leptospira/aislamiento & purificación , Leptospirosis/complicaciones , Leptospirosis/microbiología , Enfermedades Pulmonares/microbiología , Nicaragua/epidemiología , Roedores , Porcinos , Microbiología del AguaRESUMEN
Ticks and blood samples were collected from wild birds mist-netted on St. Catherine's Island, Georgia, and at the Wedge Plantation in coastal South Carolina in 1994 and 1995. Immature stages of 5 species of ixodid ticks were recovered from 10 of 148 (7%) birds belonging to 6 species in Georgia, whereas 6 ixodid species were recovered from 45 of 259 (17%) birds representing 10 avian species in South Carolina. Borrelia burgdorferi sensu lato was isolated from 27 of 120 (23%) screened ticks (Ixodes scapularis and Ixodes minor) recovered from South Carolina birds, but from none of 16 screened ticks removed from Georgia birds. This spirochete was also isolated from 1 of 97 (1%) birds in South Carolina. In 1995, neither eastern equine encephalitis (EEE) virus nor St. Louis encephalitis (SLE) virus was isolated from any of 218 bird sera screened, but serum neutralizing antibodies were found to EEE virus in 4 of 121 (3%) sera and to SLE virus in 2 of 121 (2%) sera from South Carolina. No antibody to either virus was detected in 51 avian sera screened from Georgia. Trypanosomes (probably Trypanosoma avium) were isolated from 1 of 51 (2%) birds from Georgia and from 13 of 97 (13%) birds from South Carolina. Our data suggest that some wild birds may be reservoir hosts for the Lyme disease spirochete and for encephalitis viruses in coastal Georgia and South Carolina and that migrating birds can disperse immature ticks infected with B. burgdorferi.
Asunto(s)
Anticuerpos Antivirales/aislamiento & purificación , Vectores Arácnidos , Aves/parasitología , Virus de la Encefalitis/inmunología , Ixodes , Enfermedad de Lyme/veterinaria , Trypanosoma/aislamiento & purificación , Animales , Animales Salvajes/parasitología , Vectores Arácnidos/microbiología , Vectores Arácnidos/parasitología , Vectores Arácnidos/virología , Grupo Borrelia Burgdorferi/aislamiento & purificación , Reservorios de Enfermedades/veterinaria , Encefalitis de San Luis/epidemiología , Encefalitis de San Luis/inmunología , Encefalitis de San Luis/veterinaria , Encefalitis Transmitida por Garrapatas/epidemiología , Encefalitis Transmitida por Garrapatas/inmunología , Encefalitis Transmitida por Garrapatas/veterinaria , Encefalomielitis Equina/epidemiología , Encefalomielitis Equina/inmunología , Georgia/epidemiología , Ixodes/microbiología , Ixodes/parasitología , Ixodes/virología , Enfermedad de Lyme/epidemiología , Enfermedad de Lyme/transmisión , South Carolina/epidemiologíaRESUMEN
Potosi (POT) virus (Bunyaviridae) was isolated from Aedes albopictus, an introduced Asian mosquito species, collected at a used tire yard in Potosi, Missouri (USA), in August and September, 1989. In September, 1990, small animals were trapped at the tire yard and six cattle were sampled at an adjacent farm; in November 1990 and 1991, blood samples were collected with filter paper strips from 364 hunter-killed, white-tailed deer (Odocoileus virginianus) in the region to determine the possible reservoir hosts of the virus. Deer specimens from Arkansas (n = 70), Colorado (n = 29), and Iowa (n = 763) (USA) were also analyzed. Specimens from 33 small vertebrates captured at the tire yard were negative for viruses. Only one eastern chipmunk (Tamias striatus) and none of six cattle had neutralizing (N) antibody against POT virus by the plaque-reduction serum neutralization test in Vero cell culture but 45 (25%) of 178 deer specimens in 1990 and 55 (30%) of 186 in 1991 were antibody positive. The 186 deer sera from 1991 were tested further and 29 (16%) were also N antibody positive to Cache Valley (CV) virus. From the 763 deer specimens tested from Iowa in 1993, 114 (15%) had N antibody to POT virus. Of 70 serum specimens from Arkansas deer in 1990, 33 (47%) had N antibody to POT and 15 (21%) to CV viruses; two (7%) of 29 CV negative serum specimens from Colorado deer in 1981 were serologically positive to POT virus. Three eastern chipmunks were experimentally inoculated with POT virus to determine their reservoir potential; none became viremic but all developed N antibody. Thus we propose that POT virus may be another virus regularly infecting wild deer populations but its impact on the health of these animals is unknown.
Asunto(s)
Infecciones por Arbovirus/veterinaria , Arbovirus/aislamiento & purificación , Infecciones por Bunyaviridae/veterinaria , Ciervos , Reservorios de Enfermedades , Orthobunyavirus/aislamiento & purificación , Animales , Anticuerpos Antivirales/sangre , Infecciones por Arbovirus/epidemiología , Arbovirus/inmunología , Infecciones por Bunyaviridae/epidemiología , Bovinos , Femenino , Masculino , Missouri/epidemiología , Orthobunyavirus/inmunología , Prevalencia , Sciuridae , Siphonaptera/virología , Garrapatas/virologíaRESUMEN
To determine the susceptibility of some common Colorado (USA) rodent species to Borrelia burgdorferi, pregnant Peromyscus maniculatus, Tamias minimus, and Spermophilus lateralis were trapped in May 1990 and kept in quarantine until their young were old enough to be used in the experiment. Six to eight 8-wk-old individuals of each of the Colorado species and, for comparison, eight laboratory raised P. leucopus were subcutaneously inoculated with > or = 10(5) spirochetes in 0.1 ml in July 1990. Tissue specimens were collected for isolation from these animals through April 1991. Spirochetes were isolated from blood, ear, bladder, kidney, spleen, liver, and eye in Barbour-Stoener-Kelly (BSK) medium from P. maniculatus, P. leucopus and T. minimus. Spirochetes were isolated from at least one tissue from all of these animals and no isolations were obtained from any of the S. lateralis. Thus, three of the four rodent species tested are susceptible to, and could harbor, B. burgdorferi.
Asunto(s)
Bacteriemia/veterinaria , Enfermedad de Lyme/veterinaria , Peromyscus , Enfermedades de los Roedores/inmunología , Sciuridae , Animales , Bacteriemia/inmunología , Bacteriemia/microbiología , Grupo Borrelia Burgdorferi/aislamiento & purificación , Susceptibilidad a Enfermedades , Oído/microbiología , Ojo/microbiología , Femenino , Riñón/microbiología , Hígado/microbiología , Enfermedad de Lyme/inmunología , Enfermedad de Lyme/microbiología , Masculino , Embarazo , Bazo/microbiología , Vejiga Urinaria/microbiologíaRESUMEN
Populations of the blacklegged tick, Ixodes scapularis Say, are established in western and central Wisconsin in the upper midwestern United States, but appear to be expanding geographically there. Here, we report a previously unknown population in northeastern Wisconsin. Questing I. scapularis nymphs and adults were collected by flagging vegetation from a riverine site in Marinette County, Wisconsin, in spring of 1993 and 1994. Dissection and culture of tick guts in modified Barbour-Stoenner-Kelley II medium showed that some of the ticks were infected with Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwald & Brenner, causative agent of Lyme disease. Fifteen of 30 white-footed mice, Peromyscus leucopus (Rafinesque), live-trapped at the site on 23-24 August 1994 were infested with immature I. scapularis, and ear-punch biopsies yielded B. burgdorferi cultures from 2 of the mice. However, none of 50 white-tailed deer, Odocoileus virginianus (Zimmermann), shot by hunters in Marinette County in November 1994 had I. scapularis on them, probably because no deer were shot at the same site where the tick population is located. These findings document existence of an established population of I. scapularis and a focus of Lyme disease in northeastern Wisconsin.
Asunto(s)
Grupo Borrelia Burgdorferi/aislamiento & purificación , Ixodes/microbiología , Peromyscus/parasitología , Infestaciones por Garrapatas/parasitología , Animales , Ciervos , Femenino , Masculino , Infestaciones por Garrapatas/veterinaria , WisconsinRESUMEN
To study the susceptibility of wading birds to eastern equine encephalitis (EEE) virus and to determine their potential as reservoir or amplifying hosts, fledgling glossy ibises (Plegadis falcinellus) and snowy egrets (Egretta thula) were captured in New Jersey (USA) and shipped to Colorado (USA) where they were experimentally inoculated with EEE virus. All 16 snowy egrets and 14 (93%) of 15 of the glossy ibises inoculated became viremic with moderate titers, and all survivors developed neutralizing antibody. Six ibises and two egrets died during the first week after inoculation, and EEE virus was isolated from the tissues of three birds. Our experimental results support field evidence about the relative involvement of glossy ibises and snowy egrets in the epizootiology of EEE virus in New Jersey.
Asunto(s)
Enfermedades de las Aves/inmunología , Reservorios de Enfermedades , Virus de la Encefalitis Equina del Este , Encefalomielitis Equina/veterinaria , Viremia/veterinaria , Animales , Anticuerpos Antivirales/sangre , Aves , Encéfalo/virología , Chlorocebus aethiops , Susceptibilidad a Enfermedades , Virus de la Encefalitis Equina del Este/inmunología , Virus de la Encefalitis Equina del Este/aislamiento & purificación , Encefalomielitis Equina/inmunología , Corazón/virología , Hígado/virología , Pruebas de Neutralización/veterinaria , Bazo/virología , Células Vero , Viremia/inmunologíaRESUMEN
Neotropical bats were collected from different life zones in Guatemala in 1983 and 1984 to determine the presence and distribution of antibody against 10 viruses. Bats were collected with mist nets at 13 sites in eight departments and 332 serum specimens were obtained for testing for neutralizing (N) antibody by the plaque-reduction neutralization test. Eighty-seven (26%) of the 332 bats from 16 (38%) of 42 bat species sampled were serologically positive for five of six arboviruses and for two other viruses tested. Antibodies against Venezuelan equine encephalitis (VEE) variant I-A/B, eastern equine encephalitis, western equine encephalitis, St. Louis encephalitis, vesicular stomatitis, Tacaribe, and Rio Bravo viruses were detected in resident species of bats. However, N antibodies against the enzootic strain of VEE (Mena II, variant I-E) or Nepuyo viruses were not detected.
Asunto(s)
Anticuerpos Antivirales/sangre , Quirópteros , Reservorios de Enfermedades , Virosis/veterinaria , Animales , Infecciones por Arbovirus/epidemiología , Infecciones por Arbovirus/veterinaria , Arbovirus/inmunología , Guatemala/epidemiología , Pruebas de Neutralización/veterinaria , Prevalencia , Estudios Seroepidemiológicos , Virosis/epidemiologíaRESUMEN
The vector competence of Aedes albopictus from Pine Bluff, AR, was assessed for a St. Louis encephalitis (SLE) virus strain isolated during the 1991 epidemic. Aedes albopictus were fed on hamsters with viremia levels of 10(4.6)-10(4.9) Vero cell plaque-forming units (PFU)/ml. At 7 and 15 days postbloodfeeding, transmission trials were conducted using individual suckling mice. Three of 313 Ae. albopictus were determined to be infected with SLE virus with titers of 10(6.3)-10(7.0) PFU/mosquito. At 15 days postbloodfeeding, one of 209 Ae. albopictus that refed transmitted virus resulting in a 15-day population transmission rate of 0.5%. The infection threshold (i.e., the amount of virus required to infect from 1 to 5% of mosquitoes) was determined to be approximately 10(2.3) PFU/mosquito. Virus inoculated intracoelomically into Ae. albopictus replicated and reached mean titers above 10(6.0) PFU/mosquito on day 6. The combination of low susceptibility to infection and a mammalophilic bloodfeeding pattern suggests that Ae. albopictus is unlikely to play a significant role in SLE transmission.
Asunto(s)
Aedes/virología , Virus de la Encefalitis de San Luis/aislamiento & purificación , Encefalitis de San Luis/transmisión , Insectos Vectores , Animales , Arkansas/epidemiología , Aves/virología , Pollos/virología , Cricetinae , Brotes de Enfermedades , Encefalitis de San Luis/epidemiología , Femenino , Humanos , Larva/virología , RatonesRESUMEN
Host mice (Peromyscus leucopus and Peromyscus maniculatus) were sampled throughout the state of Pennsylvania to determine the geographical and ecological distribution of the Lyme disease spirochete Borrelia burgdorferi. All 67 counties of the state were sampled. A total of 1,619 mice were captured from a total of 157 sites during the period 1990 to 1993 for an overall capture rate of 29.69%. A total of 112 (6.92%) isolations of B. burgdorferi were made. The distribution of isolations revealed the reason for the correlated distribution of human cases of Lyme disease in the state. Significantly more mice were captured and significantly more isolations were made from hemlock (Tsuga canadensis) habitat than from deciduous species forest. Nevertheless, high isolation rates from counties of the southeastern corner of the state illustrate well that hemlock habitat is not essential. Evidence suggests that in some areas, transmission between mice is occurring in some way other than through ticks as vectors. Host mice proved useful for determining the geographical and ecological distribution of B. burgdorferi.
Asunto(s)
Grupo Borrelia Burgdorferi/aislamiento & purificación , Peromyscus/microbiología , Animales , Reservorios de Enfermedades , PennsylvaniaRESUMEN
The Lyme disease spirochete, Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner, was recovered from the guts of questing Ixodes scapularis Say adults and nymphs and adult Dermacentor variabilis (Say), collected in Menominee County, Michigan, in 1992. Spirochetes were cultured successfully from guts placed in modified Barbour-Stoenner-Kelly II medium held in 5-ml cultures tubes or in 0.3-ml wells of microtiter plates. Most isolates were recovered within 2 wk of culturing at an incubation temperature of 34 degrees C. Spirochetes were recovered more rapidly in culture tubes than in microtiter plates. For determination of tick infection with B. burgdorferi, culturing was equivalent to examination of gut smears by immunofluorescence. We found the following infection rates: 57/179 I. scapularis females (31.8%), 62/204 I. scapularis males (30.4%), 9/54 I. scapularis nymphs (16.7%), and 5/383 D. variabilis adults (1.3%).
Asunto(s)
Grupo Borrelia Burgdorferi/aislamiento & purificación , Garrapatas/microbiología , Animales , Dermacentor/microbiología , Femenino , Enfermedad de Lyme/epidemiología , Enfermedad de Lyme/transmisión , Masculino , Michigan/epidemiologíaRESUMEN
Pseudorabies virus was isolated in cell culture from the brain tissue of a 3.5-year-old male Florida panther (Felis concolor coryi). The virus was not isolated from other tissues collected at necropsy. Based upon a nested polymerase chain reaction (PCR), the virus was determined to have the classical wild-type virulent genotype, glycoprotein I+ (gI+) and thymidine kinase+ (TK+).