The impact of selfies on body image satisfaction and the chain mediating role of self-objectification and narcissistic personality.

This study explores the mediating effect of self-objectification and narcissistic personality on the relationship between selfies and body image satisfaction. A total of 368 college students were administered a survey that included general information, selfie-related questions, a body image satisfaction scale, a body surveillance scale, and a narcissistic personality scale. Selfies, body image satisfaction, self- objectifica- tion, and narcissistic personality were shown to be positively correlated. Mediation modeling analysis found that selfies had a direct effect on body image satisfaction and that self-objectification and narcissistic personality mediated this relationship. Consequently, this study holds suggestions for researchers and educators searching for better exploration and attention to improve the content of education, guide students to set up a correct moral outlook, outlook on life and values.

Factors promoting resilience among breast cancer patients: a qualitative study.

OBJECTIVE: The purpose of this study was to investigate resilience factors that helped Chinese breast cancer patients adapt to the trauma in the traditional Chinese cultural context. METHOD: Checklist-guided in-depth interviews were conducted on 15 Chinese breast cancer patients recruited from two affiliated teaching hospitals. All had adapted to the illness successfully. All interviews were guided by checklist-guided interview guidelines based on the social ecosystem theory. They were recorded digitally and transcribed verbatim. Data analysis was performed using published content analysis approach. FINDINGS: The main themes found to be relevant included hope for rehabilitation, hardiness, confidence in situation, optimistic attitude to the disease, gratitude to supporters, mastery of life. Each level of these individual resource interacted with each other, which was consistent with the micro system of social ecosystem theory. CONCLUSION: This study showed that individual resource played a significant role in helping the Chinese breast cancer patients deal with the illness. There is a clear need to develop a comprehensive evaluation system to help clinical nurses assess patients' individual resource and promote better adaption to breast cancer.

Metal-to-Ligand Charge-Transfer-based Visual Detection of Alkaline Phosphatase Activity.

The ability to directly detect alkaline phosphatase (ALP) activity in undiluted serum samples is of great importance for clinical diagnosis. In this work, we report the use of the distinctive metal-to-ligand charge-transfer (MLCT) absorption properties of the Cu(BCA)2+ (BCA = bicinchoninic acid) reporter for the visual detection of ALP activity. In the presence of ALP, the substrate ascorbic acid 2-phosphate (AAP) can be enzymatically hydrolyzed to release ascorbic acid (AA), which in turn reduces Cu2+ to Cu+. Subsequently, the complexation of Cu+ with the BCA ligand generates the chromogenic Cu(BCA)2+ reporter, accompanied by a color change of colorless-to-purple of the solution with a sharp absorption band at 562 nm. The underlying MLCT-based mechanism has been demonstrated on the basis of density functional theory (DFT) calculations. Needless of any sequential multistep operations and elaborately designed colorimetric probe, the proposed MLCT-based method allows for a fast and sensitive visual detection of ALP activity within a broad linear range of 20 - 200 mU mL-1 (R2 = 0.999), with a detection limit of 1.25 mU mL-1. The results also indicate that it is highly selective and has great potential for the screening of ALP inhibitors in drug discovery. More importantly, it shows a good analytical performance for the direct detection of the endogenous ALP levels of undiluted human serum samples. Owing to the prominent simplicity and practicability, it is reasonable to conclude that the proposed MLCT-based method has a high application prospect in clinical diagnosis.

Fluorescence quenching based alkaline phosphatase activity detection.

Simple and fast detection of alkaline phosphatase (ALP) activity is of great importance for diagnostic and analytical applications. In this work, we report a turn-off approach for the real-time detection of ALP activity on the basis of the charge transfer induced fluorescence quenching of the Cu(BCDS)22- (BCDS = bathocuproine disulfonate) probe. Initially, ALP can enzymatically hydrolyze the substrate ascorbic acid 2-phosphate to release ascorbic acid (AA). Subsequently, the AA-mediated reduction of the Cu(BCDS)22- probe, which displays an intense photoluminescence band at the wavelength of 402nm, leads to the static quenching of fluorescence of the probe as a result of charge transfer. The underlying mechanism of the fluorescence quenching was demonstrated by quantum mechanical calculations. The Cu(BCDS)22- probe features a large Stokes shift (86nm) and is highly immune to photo bleaching. In addition, this approach is free of elaborately designed fluorescent probes and allows the detection of ALP activity in a real-time manner. Under optimal conditions, it provides a fast and sensitive detection of ALP activity within the dynamic range of 0-220mUmL-1, with a detection limit down to 0.27mUmL-1. Results demonstrate that it is highly selective, and applicable to the screening of ALP inhibitors in drug discovery. More importantly, it shows a good analytical performance for the direct detection of the endogenous ALP levels of undiluted human serum and even whole blood samples. Therefore, the proposed charge transfer based approach has great potential in diagnostic and analytical applications.

Metal-to-ligand charge-transfer: Applications to visual detection of ß-galactosidase activity and sandwich immunoassay.

In this work, we report a novel use of the distinctive metal-to-ligand charge-transfer (MLCT) absorption properties of the chromogenic Fe(BPDS)34- (BPDS=bathophenanthroline disulfonic acid) reporter for the visual detection of ß-galactosidase (ß-Gal) activity and sandwich immunoassay. The enzymatic hydrolysis of the substrate p-aminophenyl-ß-D-galactopyranoside can switch on the reduction of Fe3+ to Fe2+ and the subsequent complexation of Fe2+ with the BPDS ligand to generate the Fe(BPDS)34- reporter, leading to the appearance of the intense MLCT absorption band at 535nm and the colorless-to-red color change of the solution. Simply through a single step, the activity of ß-Gal can be sensitively and selectively detected within the dynamic range of 0-220mUmL-1, with a limit of detection (LOD) of 1.69mUmL-1. This approach is applicable for the visual detection of ß-Gal activities in the presence of complex human serum samples. Besides, when integrated with the sandwich immunoassay of carcinoembryonic antigen, a LOD of 1.16ngmL-1 can be achieved. In light of its prominent simplicity and practicality, our MLCT-based approach holds great potential in diagnostic and analytical applications.

Sensitive and selective colorimetric assay of alkaline phosphatase activity with Cu(II)-phenanthroline complex.

Alkaline phosphatase (ALP) plays a vital role in dephosphorylation- and phosphorylation-related cellular regulation and signaling processes. Accordingly, the development of efficient methods for ALP activity assay is of significant importance in clinical diagnosis. In this work, a simple and practical method is reported for the first time for the sensitive and selective colorimetric assay of ALP activity by exploiting a water-soluble Cu(II)-phenanthroline complex as the probe, on the basis of the distinctive metal-to-ligand charge-transfer (MLCT) properties. This method is simply built on a two-step chromogenic reaction: the enzymatic hydrolysis of the substrate ascorbic acid 2-phosphate to ascorbic acid (AA), followed by the reduction of the colorimetric probe Cu(BPDS)22- (BPDS=bathophenanthroline disulfonate) by AA to its cuprous form. The latter process triggers a turn-on spectral absorption at 424nm and a striking color change of the solution from colorless to blackish-green. Needless of complicated protocols and instrumentation, this method allows a sensitive readout of ALP activity within a wide linear range of 0-200mUmL-1, with a detection limit down to 1.25mUmL-1. Results also reveal that it is highly selective and holds great potential in ALP inhibitor efficiency evaluation. In addition, quantitative analysis of ALP activity in spiked serum samples has been realized successfully in the linear range of 0-200mUmL-1, with a detection limit of 1.75mUmL-1. Advantages of simplicity, wide linear range, high sensitivity and selectivity, low cost, and little background interference render this method great potential in practical applications.