[Chemical-toxicological diagnosis of hellebore (veratrum) poisoning]. / Khimiko-toksikologicheskaya diagnostika otravlenii chemeritsei.

The problem of laboratory diagnosis of acute and fatal poisoning by hellebore, which is possible when used in traditional medicine, the erroneous use of hellebore preparations orally or use of various types of this plant for food, remains relevant. Currently, in the practice of chemical-toxicological laboratories and the bureau of forensic medical examination there is no single approach to the laboratory diagnosis of such poisoning. The diagnosis is most often based on anamnesis. In this regard, the development and validation of a legally significant methodology for the determination of hellebore alkaloids in various biological objects seems relevant. The physicochemical and toxic properties of alkaloids of various types of hellebore are characterized. It was shown that for the identification of hellebore alkaloids, it is advisable to use HPLC-MS/MS as the most sensitive and specific instrumental method corresponding to the characteristics of hellebore alkaloids (high molecular weight, high thermal lability, high polarity).

Specifics of psychological and pedagogic correction of deviani behavior in younger adolescents with intellectual disability

Problem and aim. Deviant behavior is not only an alarming phenomenon but also a multitude of social, pedagogic and psychological problems that threaten the society and that became particularly relevant for the young adolescents with intellectual disability. Therefore, the aim of the present article is to reveal the specifics of correctional and developmental work with such children that would provide appropriate interpersonal interaction in the adolescence and the further positive socialization. Methods. The presented study was conducted in three stages (exploratory-preparatory, experimental and control-generalizing stages) with the use of the Buss-Durkee Hostility Inventory. Results and discussion. The results showed that the predominant from of the deviant behavior was hostility and violence, as well as a tendency for addictive behavior. Diagnostics of the dominant indices of hostility and aggressiveness demonstrated that the aggressiveness index dominated in the majority of adolescents. The majority of adolescents had a very high or high level of indirect hostility and assault, along with suspicion. The correctional program provides correctional effect for the adolescents with low and medium level of proneness to the deviant behavior

[Phospholipase D: its role in metabolism processes and disease development]. / Fosfolipaza D: ee rol' v protsessakh metabolizma i razvitii patologicheskikh sostoianii.

Phospholipase D (PLD) is one of the key enzymes that catalyzes the hydrolysis of cell membrane phospholipids. In this review current knowledge about six human PLD isoforms, their structure and role in physiological and pathological processes is summarized. Comparative analysis of PLD isoforms structure is presented. The mechanism of the hydrolysis and transphosphatidylation performed by PLD is described. The PLD1 and PLD2 role in the pathogenesis of some cancer, infectious, thrombotic and neurodegenerative diseases is analyzed. The prospects of PLD isoform-selective inhibitors development are shown in the context of the clinical usage and the already-existing inhibitors are characterized. Moreover, the formation of phosphatidylethanol (PEth), the alcohol abuse biomarker, as the result of PLD-catalyzed phospholipid transphosphatidylation is considered.