Asunto(s)
Neoplasias Óseas/diagnóstico por imagen , Carcinoma/diagnóstico , Neoplasias Pulmonares/diagnóstico por imagen , Células Oxífilas/patología , Neoplasias de la Tiroides/diagnóstico , Anciano , Neoplasias Óseas/secundario , Resultado Fatal , Humanos , Neoplasias Pulmonares/secundario , Masculino , Tomografía Computarizada por Rayos XRESUMEN
The genetically heterogeneous taxon Candida parapsilosis was recently reclassified into three species: Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis. The prevalences of these species among 141 bloodstream isolates tested in Brazil were 88% for C. parapsilosis, 9% for C. orthopsilosis, and 3% for C. metapsilosis. Except for three C. orthopsilosis isolates that were considered resistant to 5-flucytosine, all isolates representing the different species of this complex were susceptible to polyenes, triazoles and caspofungin.
Asunto(s)
Antifúngicos/farmacología , Candida/efectos de los fármacos , Candidiasis/epidemiología , Fungemia/epidemiología , Antifúngicos/uso terapéutico , Brasil/epidemiología , Candida/clasificación , Candida/aislamiento & purificación , Candidiasis/microbiología , Caspofungina , ADN de Hongos/análisis , Farmacorresistencia Fúngica , Equinocandinas/farmacología , Equinocandinas/uso terapéutico , Flucitosina/farmacología , Flucitosina/uso terapéutico , Fungemia/microbiología , Humanos , Lipopéptidos , Pruebas de Sensibilidad Microbiana , Técnicas de Tipificación Micológica , Polienos/farmacología , Polienos/uso terapéutico , Vigilancia de la Población , Prevalencia , Triazoles/farmacología , Triazoles/uso terapéuticoRESUMEN
Proper morphology is essential for the ability of Candida albicans to switch between yeast and hyphae and thereby sustain its virulence. Here we identified, by differential screening, a novel C. albicans AAA ATPase encoding gene, CaYLL34 (RIX7), with enhanced expression in hyphae. Phylogenetic analysis suggests that CaYLL34 belongs to a "VCP-like" subgroup of AAA ATPases essential for yeast viability and contains a bipartite nuclear localization signal. Inactivation of one copy of CaYLL34, by the URA-Blaster method, generated the heterozygous mutant strain M61. This strain has severe phenotypic alterations, such as a highly increased vacuole, abnormal cell shape and reduced growth in different conditions. Also, major pathogenicity factors are affected in M61, for instance, a significant decrease of hypha formation (>90%), surface biofilm adhesion (86%) and secreted aspartyl proteinase activity (76.5%). Our results show that the partial impairment of CaYll34p cellular levels is sufficient to affect the proper cellular morphology and pathogenicity factors and suggest that this protein is required for biogenesis of ribosomal subunits. Accordingly, we propose that the product of CaYLL34 could be tested as a novel target for antifungal drugs.