Molecular characterization of Dipetalonema yatesi from the black-faced spider monkey (Ateles chamek) with phylogenetic inference of relationships among Dipetalonema of Neotropical primates.

Species of the genus Dipetalonema are parasitic nematodes of the family Onchocercidae (Nematoda; Filarioidea) which infect the peritoneal cavity of Neotropical primates. Of these, six species have been taxonomically described, two of these have been reported infecting the black-faced spider monkey (Ateles chamek): Dipetalonema gracile and Dipetalonema yatesi. Description of Dipetalonema species have been based on morphological characteristics, and their phylogenetic relationships remain unresolved. A few molecular studies have been carried out in Dipetalonema spp. infecting Neotropical primates. Seven filarioid nematodes (6 females and one male) recovered from one A. chamek in the Peruvian Amazon rainforest were morphologically identified as D. yatesi and molecularly characterized. A multi-locus genetic analysis of nuclear ribosomal region (18S) and mitochondrial (cox1, 12S, and nad5) gene sequences supported D. yatesi as a distinct lineage and yielded a highly resolved phylogenetic lineage tree for this filarioid genus of Neotropical primates. Our results highlighted that Dipetalonema species are divided in two well-supported clades, one containing D. yatesi and D. caudispina, and the second containing D. robini, D. gracile, and D. graciliformis. Due to sequence ambiguities from GenBank entries, relationships among isolates of D. gracile and D. graciliformis cannot be fully resolved, which requires further investigation. However, this suggests that these could represent a species complex. Our study confirms that D. yatesi is a valid species and constitutes the first molecular phylogenetic analysis of this parasite in black-faced spider monkeys.

Social Support in Patients With Sexual Dysfunction After Non-Nerve-Sparing Radical Prostatectomy: A Qualitative Study.

This study aimed to explore men's experiences of social support after non-nerve-sparing radical prostatectomy. A qualitative study based on Gadamer's hermeneutic phenomenology was designed. In-depth interviews were conducted with 16 men who had undergone a non-nerve-sparing radical prostatectomy. Data analysis was performed using ATLAS.ti software. From this analysis, two main themes emerged: "The partner as a source of support and conflict after a prostatectomy," which includes empathetic reconnection with the partner and changes in sexual and cohabitation patterns and "The importance of social and professional circles," which addresses the shortcomings of the healthcare system in terms of sexual information and counseling as well as the role of friends within social support. The study suggests the need to establish interventions that address interpersonal communication and attention to social and informational support and include both the patient and those closest to them.

Experiências sexuais após prostatectomia radical não poupadora de nervos / Experiencias sexuales después de prostatectomía radical sin conservación de bandeletas neurovasculares / Sexual experiences after non-nerve sparing radical prostatectomy

Resumo Objetivo O objetivo deste estudo foi explorar as experiências e expectativas de pacientes submetidos a prostatectomia radical não poupadora de nervos. Métodos Estudo qualitativo, descritivo-exploratório, baseado na fenomenologia hermenêutica. Foram realizadas dezesseis entrevistas em profundidade entre fevereiro e dezembro de 2017 e análise indutiva dos dados. Resultados Dois temas que refletem as experiências dos pacientes emergiram da análise: (1) As mudanças sexuais como fator-chave da resposta adaptativa, com os subtemas "Dificuldades sexuais após a cirurgia" e "Questões com relacionamentos íntimos e bem-estar psicossocial"; (2) Prostatectomia: a necessidade de adaptação a uma sexualidade holística, com os subtemas "Questionamento das experiências a partir do comportamento sexual atual" e "Adaptação da sexualidade e a intimidade após a cirurgia". Conclusão Pacientes submetidos à prostatectomia enfrentam dificuldades sexuais como disfunção erétil. Essas mudanças causam problemas nos relacionamentos sexuais e íntimos que afetam seu bem-estar. Diante dessa situação, alguns participantes questionam suas práticas sexuais habituais e tentam se adaptar a uma sexualidade holística não centrada no coito, incorporando formas inovadoras de sexualidade. Conhecer as experiências de homens submetidos a prostatectomia radical pode ajudar os profissionais de saúde a oferecer novas estratégias para lidar com as mudanças ambientais envolvidas na cirurgia da próstata.

Resumen Objetivo El objetivo de este estudio fue analizar las experiencias y expectativas de pacientes sometidos a prostatectomía radical sin conservación de bandeletas neurovasculares. Métodos Estudio cualitativo, descriptivo-exploratorio, basado en la fenomenología hermenéutica. Se realizaron 16 entrevistas en profundidad entre febrero y diciembre de 2017 y un análisis inductivo de los datos. Resultados A partir del análisis, surgieron dos temas que reflejan las experiencias de los pacientes: (1) Los cambios sexuales como factor clave de la respuesta adaptativa, con dos subtemas "Dificultades sexuales después de la cirugía" y "Cuestiones sobre relaciones íntimas y bienestar psicosocial" y (2) Prostatectomía: necesidad de adaptación a una sexualidad holística, con los subtemas "Cuestionamiento de las experiencias a partir del comportamiento sexual actual" y "Adaptación de la sexualidad e intimidad después de la cirugía". Conclusión Los pacientes sometidos a prostatectomía enfrentan dificultades sexuales como disfunción eréctil. Estos cambios causan problemas en las relaciones sexuales e íntimas que afectan su bienestar. Ante esta situación, algunos participantes cuestionan sus prácticas sexuales habituales e intentan adaptarse a una sexualidad holística no centrada en el coito, incorporando formas innovadoras de sexualidad. Conocer las experiencias de hombres sometidos a prostatectomía radical puede ayudar a los profesionales de la salud a ofrecer nuevas estrategias para lidiar con los cambios ambientales relacionados con la cirugía de próstata.

Abstract Objective The aim of this study was to explore the experiences and expectations of patients who underwent non-nerve sparing radical prostatectomy. Methods A qualitative descriptive, exploratory, study, based on hermeneutic phenomenology. Sixteen in-depth interviews were carried out between February and December 2017. An inductive analysis of data was performed. Results Two themes reflecting the patients' experiences emerged from the analysis: (1) Sexual changes as a key factor of the adaptive response, with the subthemes "Sexual difficulties after surgery" and "Issues with intimate relationships and psychosocial wellbeing". (2) Prostatectomy: the need of adaptation to a holistic sexuality, with the subthemes "Questioning the experiences from the current sexual behavior" and "Adapting sexuality and intimacy after surgery". Conclusion Patients undergoing prostatectomy face sexual difficulties such as erectile dysfunction. These changes cause problems in sexual and intimate relationships that affect their wellbeing. Given this situation, some participants question their usual sexual practices and try to adapt to a holistic sexuality not centered on coitus by incorporating innovative forms of sexuality. Knowing the experiences of men who underwent radical prostatectomy might help healthcare workers to provide new strategies for coping with the environmental changes involved in prostate surgery.