Wastewater-based epidemiology for early warning of SARS-COV-2 circulation: A pilot study conducted in Sicily, Italy.

There is increasing evidence of the use of wastewater-based epidemiology to integrate conventional monitoring assessing disease symptoms and signs of viruses in a specific territory. We present the results of SARS-CoV-2 environmental surveillance activity in wastewater samples collected between September 2020 and July 2021 in 9 wastewater treatment plants (WTPs) located in central and western Sicily, serving over 570,000 residents. The presence of SARS-CoV-2, determined in 206 wastewater samples using RT-qPCR assays, was correlated with the notified and geo-referenced cases on the areas served by the WTPs in the same study period. Overall, 51% of wastewater samples were positive. Samples were correlated with 33,807 SARS-CoV-2 cases, reported in 4 epidemic waves, with a cumulative prevalence of 5.9% among Sicilian residents. The results suggest that the daily prevalence of SARS-CoV-2 active cases was statistically significant and higher in areas with SARS-CoV-2 positive wastewater samples. According to these findings, the proposed method achieves a good sensitivity profile (78.3%) in areas with moderate or high viral circulation (≥133 cases/100,000 residents) and may represent a useful tool in the management of epidemics based on an environmental approach, although it is necessary to improve the accuracy of the process.

Robotic-assisted laparoscopic prostatectomy in Italy: perioperative data on the first 150 patients.

BACKGROUND: In 2004 we performed the first robot-assisted laparoscopic prostatectomy (RALP) at Aosta, Italy. METHODS: Data about our first 150 RALPs are presented. RESULTS: Mean patient age was 65.2 (range 51-75) years; mean preoperative PSA level 7.9 (range 1.8-25.0) ng/ml. Nerve sparing was performed in 109 cases (72.7%) with lymph nodes dissection in 27 (18.0%). Operative time: patients 1-50, mean 213.3 (range 185-290) min; patients 51-100, 207.3 (range 185-335) min; patients 101-150, 171.7 (range 123-270) min. Two procedures were converted to open. Mean blood loss was 235 (range 20-1000) ml with two blood transfusions. One patient was reoperated for anastomotic leakage. Four patients were treated by colostomy and rectal suture for rectal injury (n = 3) or recto-urethral fistula (n = 1). The overall complication incidence was 13.3% (20 events), while mortality was nil. CONCLUSIONS: The incidence of complications is slightly higher than in major pilot centres but it is comparable, hence RALP also appears feasible in our italian peripheral centre.