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1.
Acta Neurochir (Wien) ; 165(7): 1881-1889, 2023 07.
Artículo en Inglés | MEDLINE | ID: mdl-37178247

RESUMEN

BACKGROUND: Since its approval by the US Food and Drug Administration (FDA) in 2018, the flow disruptor Woven EndoBridge (WEB) device has become increasingly popular for the endovascular treatment of unruptured and ruptured cerebral aneurysms. However, the occlusion rates seem rather low and the retreatment rates rather high compared to other treatment methods. For initially ruptured aneurysms, a retreatment rate of 13 % has been reported. A variety of retreatment strategies has been proposed; however, there is a paucity of data concerning microsurgical clipping of WEB-pretreated aneurysms, especially previously ruptured ones. Thus, we present a single-center series of five ruptured aneurysms treated with the WEB device and retreated with microsurgical clipping. METHODS: A retrospective study including all patients presenting with a ruptured aneurysm undergoing WEB treatment at our institution between 2019 and 2021 was performed. Subsequently, all patients with an aneurysm remnant or recurrence of the target aneurysm retreated with microsurgical clipping were identified. RESULTS: Overall, five patients with a ruptured aneurysm treated with WEB and retreated with microsurgical clipping were included. Besides one basilar apex aneurysm, all aneurysms were located at the anterior communicating artery (AComA) complex. All aneurysms were wide-necked with a mean dome-to-neck ratio of 1.5. Clipping was feasible and safe in all aneurysms, and complete occlusion was achieved in 4 of 5 aneurysms. CONCLUSIONS: Microsurgical clipping for initially ruptured WEB-treated aneurysms is a feasible, safe, and effective treatment method in well-selected patients.


Asunto(s)
Aneurisma Roto , Embolización Terapéutica , Procedimientos Endovasculares , Aneurisma Intracraneal , Humanos , Estudios Retrospectivos , Procedimientos Endovasculares/métodos , Aneurisma Intracraneal/diagnóstico por imagen , Aneurisma Intracraneal/cirugía , Resultado del Tratamiento , Aneurisma Roto/diagnóstico por imagen , Aneurisma Roto/cirugía , Embolización Terapéutica/métodos , Retratamiento
2.
AJNR Am J Neuroradiol ; 42(11): 1956-1961, 2021 11.
Artículo en Inglés | MEDLINE | ID: mdl-34556476

RESUMEN

BACKGROUND AND PURPOSE: The acute phase of aneurysmal SAH is characterized by a plethora of impending complications with the potential to worsen patients' outcomes. The aim of this study was to evaluate whether an elaborated CTP-based imaging protocol during the acute aneurysmal SAH phase is able to prevent delayed infarctions and contribute to a better outcome. MATERIALS AND METHODS: In 2012, an elaborated CTP-based protocol was implemented for the management of patients with aneurysmal SAH. Retrospective analysis of patients with aneurysmal SAH treated from 2010 to 2013 was performed, comparing the patients treated before (group one, 2010-2011) with those treated after the protocol implementation (group two, 2012-2013) with regard to delayed infarctions and outcome according to the mRS at 3-months' follow-up. RESULTS: A total of 133 patients were enrolled, of whom 57 were included in group 1, and 76, in group 2. There were no significant differences between the groups concerning baseline characteristics. In the multivariate analysis, independent predictors of a good outcome (mRS ≤ 2) were younger age (P < .001), lower World Federation of Neurosurgical Societies grade (P < .001), absence of delayed infarction (P = .01), and management according to the CTP protocol (P = .01). Larger or multiple infarctions occurred significantly more often in group 1 compared with group 2 (88% versus 33% of all delayed infarctions, P = .03). The outcome in group 2 was significantly better compared with group 1 (P = .005). CONCLUSIONS: The findings suggest that implementation of an elaborated CTP protocol is associated with a better outcome. An earlier initiation of further diagnostics and treatment with prevention of large territorial and/or multiple infarctions might have led to this finding.


Asunto(s)
Hemorragia Subaracnoidea , Humanos , Perfusión , Estudios Retrospectivos , Hemorragia Subaracnoidea/diagnóstico por imagen , Hemorragia Subaracnoidea/terapia , Tomografía Computarizada por Rayos X , Resultado del Tratamiento
3.
Acta Neurochir (Wien) ; 160(8): 1653-1660, 2018 08.
Artículo en Inglés | MEDLINE | ID: mdl-29948299

RESUMEN

BACKGROUND: Giant cavernous carotid aneurysms (GCCAs) usually exert substantial mass effect on adjacent intracavernous cranial nerves. Since predictors of cranial nerve deficits (CNDs) in patients with GCCA are unknown, we designed a study to identify associations between CND and GCCA morphology and the location of mass effect. METHODS: This study was based on data from the prospective clinical and imaging databases of the Giant Intracranial Aneurysm Registry. We used magnetic resonance imaging and digital subtraction angiography to examine GCCA volume, presence of partial thrombosis (PT), GCCA origins, and the location of mass effect. We also documented whether CND was present. RESULTS: We included 36 GCCA in 34 patients, which had been entered into the registry by eight participating centers between January 2009 and March 2016. The prevalence of CND was 69.4%, with one CND in 41.7% and more than one in 27.5%. The prevalence of PT was 33.3%. The aneurysm origin was most frequently located at the anterior genu (52.8%). The prevalence of CND did not differ between aneurysm origins (p = 0.29). Intracavernous mass effect was lateral in 58.3%, mixed medial/lateral in 27.8%, and purely medial in 13.9%. CND occurred significantly more often in GCCA with lateral (81.0%) or mixed medial/lateral (70.0%) mass effect than in GCCA with medial mass effect (20.0%; p = 0.03). After adjusting our data for the effects of the location of mass effect, we found no association between the prevalence of CND and aneurysm volume (odds ratio (OR) 1.30 (0.98-1.71); p = 0.07), the occurrence of PT (OR 0.64 (0.07-5.73); p = 0.69), or patient age (OR 1.02 (95% CI 0.95-1.09); p = 0.59). CONCLUSIONS: Distinguishing between medial versus lateral location of mass effect may be more helpful than measuring aneurysm volumes or examining aneurysm thrombosis in understanding why some patients with GCCA present with CND while others do not. CLINICAL TRIAL REGISTRATION NO: NCT02066493 ( clinicaltrials.gov ).


Asunto(s)
Angiografía de Substracción Digital/métodos , Arteria Carótida Interna/diagnóstico por imagen , Nervios Craneales/patología , Aneurisma Intracraneal/patología , Imagen por Resonancia Magnética/métodos , Adulto , Anciano , Arteria Carótida Interna/patología , Nervios Craneales/diagnóstico por imagen , Femenino , Humanos , Aneurisma Intracraneal/diagnóstico por imagen , Masculino , Persona de Mediana Edad
4.
Neurosurg Rev ; 40(4): 655-661, 2017 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-28185018

RESUMEN

Decompressive hemicraniectomy (DH) plus duroplasty was demonstrated to be effective for treating critically elevated intracranial pressure (ICP). In order to shorten operation time and to avoid the use of autologous or heterologous material, durotomy has been introduced as an alternative to duroplasty. Only limited data is available on the effect of DH and durotomy on the increased ICP in traumatic brain injury (TBI). Therefore, we collected consecutive intraoperative ICP readings during the different steps of DH and durotomy in TBI patients. Eighteen patients with TBI and uncontrollable ICP increase (measured by either an intraparenchymal or an intraventricular ICP probe) underwent DH and durotomy. ICP readings as well as mean arterial blood pressure (MAP) and arterial PCO2 were obtained during defined stages of the operation. Surgical complications of the durotomy itself and of cranioplasty after 3 months were recorded. The outcome was assessed prior to cranioplasty using the Glasgow Outcome Scale (GOS). ICP dropped significantly during surgery from a mean of 41 ( ± 16.2) mmHg at the beginning to a mean of 11.8 ( ± 7.5) mmHg at the end (p ≤ 0.001). A first significant ICP-decrease to a mean of 18 ( ± 10.8) mmHg (p ≤ 0.001) was detected after removal of the bone flap, and a second significant ICP-decrease to a mean of 10.6 ( ± 5.3) mmHg (p < 0.001) during durotomy. The mean operation time was 115.3 min ( ± 49.6). Five patients (28%) died; seven patients (39%) had a good outcome (GOS 5). There were no relevant complications associated to durotomy. Durotomy after DH is a safe and straightforward procedure, which significantly lowers critically increased ICP in patients with TBI. Although no graft is used, dural preparation for cranioplasty at 3 months is easily possible.


Asunto(s)
Lesiones Traumáticas del Encéfalo/cirugía , Craneotomía , Descompresión Quirúrgica , Hipertensión Intracraneal/cirugía , Adulto , Anciano , Lesiones Traumáticas del Encéfalo/complicaciones , Lesiones Traumáticas del Encéfalo/fisiopatología , Duramadre/cirugía , Femenino , Escala de Consecuencias de Glasgow , Humanos , Hipertensión Intracraneal/diagnóstico , Hipertensión Intracraneal/etiología , Presión Intracraneal/fisiología , Masculino , Persona de Mediana Edad , Procedimientos Neuroquirúrgicos , Colgajos Quirúrgicos , Resultado del Tratamiento
5.
Neurosurg Rev ; 38(1): 191-5; discussion 195, 2015 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-25242202

RESUMEN

Studies on immediate failed back surgery syndrome (iFBSS) following lumbar microdiscectomy are rare. Our aim is to describe the incidence and the causes of these immediate failures to define the value of radiological imaging for identification of the underlying pathology and to propose a management algorithm. We defined iFBSS as persistence, deterioration or recurrence (during hospital stay) of radicular pain and/or sensorimotor deficits and/or sphincter dysfunction after microdiscectomy, which was uneventful from the surgeon's perspective. The medical records of 1546 patients undergoing discectomy for mediolateral lumbar disc herniations were screened for iFBSS. The pre- and postoperative imaging, surgical records, therapy and outcome of patients with iFBSS were reviewed. Forty-four of 1546 patients (2.8%) with iFBSS were identified. All patients underwent reoperation. Overseen disc material/re-herniation (n = 22), epidural hematoma (n = 6), inadequate decompression of accompanying recessal stenosis (n = 2) and dural tear with fascicle herniation (n = 1) were found to be causative. In 13 patients, who revealed no clear pathology intraoperatively, we diagnosed a battered root syndrome (nerve root swelling due to excessive surgical manipulation). The correct diagnosis could be established by neuroradiological imaging in 25 of 43 radiologically investigated patients (57%). In our study, the radiological workup was of limited value for the correct differentiation of the various aetiologies of iFBSS. Therefore, the authors believe that the treatment strategy should strongly rely on the clinical presentation. To avoid unnecessary surgery in cases of battered root syndrome, we propose to proceed to reoperation only in patients with new or persistent radiculopathy despite adequate antiedematous medical therapy.


Asunto(s)
Discectomía , Desplazamiento del Disco Intervertebral/epidemiología , Vértebras Lumbares/cirugía , Adulto , Anciano , Descompresión Quirúrgica/métodos , Femenino , Humanos , Incidencia , Desplazamiento del Disco Intervertebral/complicaciones , Masculino , Persona de Mediana Edad , Reoperación , Resultado del Tratamiento
6.
Eur J Radiol ; 83(10): 1881-9, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-25052872

RESUMEN

OBJECT: Cerebral vasospasm (CV) following subarachnoid hemorrhage (SAH) implies high risk for secondary ischemia. It requires early diagnosis to start treatment on time. We aimed to assess the utility of "whole brain" VPCT for detecting localization and characteristics of arterial vasospasm. METHODS: 23 patients received a non-enhanced CT, VPCT and CTA of the brain. The distribution of ischemic lesions was analyzed on 3D-perfusion-parameter-maps of CBF, CBV, MTT, TTS, TTP, and TTD. CT-angiographic axial and coronal maximum-intensity-projections were reconstructed to determine arterial vasospasm. CT-data was compared to DSA, if performed additionally. Volume-of-interest placement was used to obtain quantitative mean VPCT values. RESULTS: 82% patients (n=19) had focal cerebral hypoperfusion. 100% sensitivity and 100% specificity was found for TTS (median 1.9s), MTT (median 5.9s) and TTD (median 7.6s). CBV showed no significant differences. In 78% (n=18) focal vessel aberrations could be detected either on CTA or DSA or on both. CONCLUSION: VPCT is a non-invasive method with the ability to detect focal perfusion deficits almost in the whole brain. While DSA remains to be the gold standard for detection of CV, VPCT has the potential to improve noninvasive diagnosis and treatment decisions.


Asunto(s)
Angiografía Cerebral/métodos , Imagenología Tridimensional , Hemorragia Subaracnoidea/diagnóstico por imagen , Tomografía Computarizada por Rayos X/métodos , Vasoespasmo Intracraneal/diagnóstico por imagen , Adulto , Anciano , Anciano de 80 o más Años , Medios de Contraste , Diagnóstico Diferencial , Femenino , Humanos , Yopamidol/análogos & derivados , Masculino , Persona de Mediana Edad , Interpretación de Imagen Radiográfica Asistida por Computador , Sensibilidad y Especificidad , Hemorragia Subaracnoidea/complicaciones , Vasoespasmo Intracraneal/etiología
7.
Berl Munch Tierarztl Wochenschr ; 114(3-4): 112-6, 2001.
Artículo en Alemán | MEDLINE | ID: mdl-11314581

RESUMEN

Possibilities for disinfection of the developmental stages from the housefly (Musca domestica) were investigated under laboratory conditions. The developmental stages (eggs, larvae I, II, III, pupae and adults) were sprayed with solutions of disinfectants on the basis of p-chlorine-m-cresol and o-phenylphenol at different concentrations (0.025%-3%). The effectiveness of both disinfectants was established by determining the emergence rate of eggs and pupae and of the live evidence of larvae and adults. Transmission electron microscopic investigations of the cover of the eggs revealed the bactericidal effect of both disinfectants. These results show the necessity of extending its use also to noxious arthropods.


Asunto(s)
Desinfectantes/farmacología , Moscas Domésticas , Animales , Compuestos de Bifenilo/farmacología , Cresoles/farmacología , Moscas Domésticas/efectos de los fármacos , Moscas Domésticas/crecimiento & desarrollo , Microscopía Electrónica , Resultado del Tratamiento
8.
Berl Munch Tierarztl Wochenschr ; 111(7-8): 291-4, 1998.
Artículo en Alemán | MEDLINE | ID: mdl-9741186

RESUMEN

The devitalizing effectiveness of 5 patterns of the disinfectant Neopredisan (active principle: p-chlorine-m-cresol) was tested to eggs of Ascaris suum in coincidence with the "guidelines for the testing of chemical disinfectants" of the German Veterinary Society (GVS). In the suspension-test all examined patterns indicated a 100% inhibition of development Ascaris suum-eggs. In the germ carrier test all patterns were in conformity with the necessities of the GVS-guidelines. Also in the modified testing method with a temperature in the refrigerator of +10 degrees C Neopredisan showed a reliable (100%) efficacy. The inhibition of development was clearly delayed with an admixture of 20% faeces to the suspension of eggs. In a suspension-test with Neopredisan 140 in 3% solution after 15 minutes with embryonated eggs a nearly 100% ovicide and larvicide respectively efficacy was proved. The investigations show, Neopredisan is of value as a suitable disinfectant in the complex of the combat against ascariasis in the breeding of pigs.


Asunto(s)
Ascaris suum/efectos de los fármacos , Cresoles/farmacología , Desinfectantes/farmacología , Animales , Óvulo/efectos de los fármacos , Estándares de Referencia , Porcinos
9.
J Cataract Refract Surg ; 24(7): 964-7, 1998 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-9682119

RESUMEN

PURPOSE: To compare the effect of latanoprost 0.005% with that of a placebo (balanced salt solution [BSS]) applied after phacoemulsification on intraocular pressure (IOP). SETTING: Pasco Eye Institute, New Port Richey, Florida, USA. METHODS: A group of patients having cataract extraction by phacoemulsification was randomized following surgery to receive one drop of latanoprost 0.005% (1.5 micrograms) or a placebo (BSS). Exclusion criteria included ocular diagnosis in addition to cataract, previous eye surgery, history of glaucoma, previous use of glaucoma medications, or vitreous loss during surgery. Standard phacoemulsification was performed through a scleral tunnel approach and a one-piece, poly(methyl methacrylate) intraocular lens implanted in the capsular bag. Approximately 24 hours after surgery, IOP was measured with a Goldmann applanation tonometer by the surgeon. The anterior chamber reaction was qualitatively graded from 1+ to 4+. RESULTS: The study included 103 eyes (latanoprost = 53; control = 50). Latanoprost treatment resulted in significantly lower postoperative (IOP) (16.4 mm Hg +/- 3.7 [SD]) than preoperative IOP (17.9 +/- 3.0 mm Hg) (P < .025). There was no decrease in postoperative IOP in the control group (18.2 +/- 3.5 mm Hg) compared with preoperative IOP (18.3 +/- 2.6 mm Hg). When two groups were compared, postoperative IOP after treatment with latanoprost was significantly less than control IOP (P < .01). Preoperative IOP was not significantly different in the placebo and latanoprost groups. Anterior chamber reaction was not increased by latanoprost; it averaged 1+ in both groups. CONCLUSIONS: Latanoprost may pharmacologically enhance uveoscleral outflow immediately after cataract extraction. In this study, latanoprost was a safe, effective method of reducing postoperative IOP.


Asunto(s)
Presión Intraocular/efectos de los fármacos , Hipertensión Ocular/tratamiento farmacológico , Facoemulsificación/efectos adversos , Prostaglandinas F Sintéticas/uso terapéutico , Método Doble Ciego , Femenino , Humanos , Latanoprost , Implantación de Lentes Intraoculares , Masculino , Hipertensión Ocular/etiología , Soluciones Oftálmicas , Polimetil Metacrilato , Prostaglandinas F Sintéticas/administración & dosificación , Tonometría Ocular , Resultado del Tratamiento
10.
South Med J ; 87(7): 685-8, 1994 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-8023199

RESUMEN

Lithium therapy for bipolar illness is well accepted, despite some difficulties with its use. The administration of anticonvulsants has increased over the past 20 years. This paper outlines advantages and problems associated with anticonvulsant therapy for mood disorders.


Asunto(s)
Afecto , Anticonvulsivantes/uso terapéutico , Trastorno Bipolar/tratamiento farmacológico , Litio/uso terapéutico , Trastorno Bipolar/clasificación , Trastorno Bipolar/psicología , Carbamazepina/uso terapéutico , Humanos , Ácido Valproico/uso terapéutico
11.
J Mol Biol ; 238(2): 187-98, 1994 Apr 29.
Artículo en Inglés | MEDLINE | ID: mdl-8158648

RESUMEN

Filamentous phage pIV is an outer membrane protein required for phage assembly and secretion. Chemical cross-linking and sedimentation experiments have been used to demonstrate that pIV from f1-infected Escherichia coli exists as a homo-multimer, probably composed of 10 to 12 subunits. pIV secreted from spheroplasts remains soluble and does not form multimers. Synthesis of pIV from distantly related filamentous phages or from a bacterial homolog that participates in a specialized form of extra-cellular protein secretion in the same cell with pIVf1 resulted in the formation of mixed multimers. This suggests that the homologous proteins themselves form homo-multimers. These structures could form gated channels that conduct assembling phage or specific substrate proteins across the outer membrane to the extracellular milieu.


Asunto(s)
Colifagos/química , Proteínas Virales/química , Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Biopolímeros , Centrifugación por Gradiente de Densidad , Reactivos de Enlaces Cruzados , Dickeya chrysanthemi/química , Escherichia coli/metabolismo , Inovirus/química , Pruebas de Precipitina , Esferoplastos/metabolismo , Proteínas Virales/metabolismo
12.
Appl Parasitol ; 34(4): 279-82, 1993 Nov.
Artículo en Alemán | MEDLINE | ID: mdl-8298660

RESUMEN

A strain of Babesia divergens was propagated by inoculation of 1.5 x 10(7) parasitized erythrocytes in 30 passages under same conditions on altogether 150 jirds. The developmental stages of Babesia divergens revealed pleomorphism in the erythrocytes of the jird. Single parasites were observed as being pyriform, globular, budding, ring-like or amoebid. The shape of dividing parasites varied between the spherical and the pyriform. The stages in the erythrocytes were localized predominantly intermedia. The reduction of the number of dividing forms and the increase of single forms occurred with increasing numbers of passages. Length and breadth of double pyriforms increased by approximately 20%.


Asunto(s)
Babesia/crecimiento & desarrollo , Gerbillinae/parasitología , Animales , Babesia/citología , Células Cultivadas , Modelos Animales de Enfermedad , Eritrocitos/parasitología , Pase Seriado
13.
Appl Parasitol ; 34(1): 11-8, 1993 Feb.
Artículo en Alemán | MEDLINE | ID: mdl-8508216

RESUMEN

The quantitative valuation of efficiency of an Eimeria tenella radiovaccine was performed on the basis of 21 tests under floor-pen conditions and on 34 tests under the conditions of industrial broiler production using the vaccination index, the recycling index and coccidiosis protection index. The immunogenic quality of the vaccine was classified quantitatively by the indices 1 to 4, in which the classes 1 and 2 fulfilled the requirements. Under floor-pen conditions the valuation in the classes 1 and 2 was ascertained on the vaccination index with 76.2%, on the recycling index with 44.4% and on the coccidiosis protection index with 100%. Under the conditions of the industrial mast these values amounted 66.7%, 64.7% and 66.7%.


Asunto(s)
Pollos , Coccidiosis/veterinaria , Eimeria tenella/inmunología , Enfermedades de las Aves de Corral/prevención & control , Vacunas Antiprotozoos , Animales , Coccidiosis/prevención & control , Estudios de Evaluación como Asunto , Vacunación/veterinaria
14.
Angew Parasitol ; 33(4): 230-4, 1992 Nov.
Artículo en Alemán | MEDLINE | ID: mdl-1456468

RESUMEN

A pure strain of Eimeria mitis was obtained from a crude field isolate by single oocyst inoculation of chicken. The strain was identified as Eimeria mitis by the morphology of the oocysts. Sporulated oocysts measured 16.1 +/- 2.1 microns in length and 13.6 +/- 1.0 microns in width. The shape index was 1.18. The prepatent period was 4 days and patency lasted 12 to 13 days. The highest number of oocysts was shed at day 6 after infection of chicken with 1 x 10(4), 5 x 10(4), or 1 x 10(5) oocysts and at day 8 after infection with 5 x 10(5) oocysts. A single infection of chicken with 1 x 10(4), 5 x 10(4), 1 x 10(5), or 5 x 10(5) oocysts resulted in reproduction values of 1:118,662, 1:15,720, 1:9,977 and 1:731, respectively.


Asunto(s)
Pollos/parasitología , Coccidiosis/veterinaria , Eimeria/ultraestructura , Enfermedades de las Aves de Corral/parasitología , Animales , Coccidiosis/parasitología , Eimeria/fisiología , Reproducción
15.
Gene ; 118(1): 93-5, 1992 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-1324873

RESUMEN

The TnphoA transposon constructed by Manoil and Beckwith [Proc. Natl. Acad. Sci. USA 82 (1985) 8129-8133] has been modified to permit easy isolation of single-stranded (ss) DNA of target plasmids. The intergenic region (IG) of filamentous phage f1, which consists of the phage origin of replication and packaging signal, was inserted into a nonessential region of TnphoA. This modified transposon should be useful for the analysis of genes cloned in plasmids that lack a filamentous phage IG. Transposition of TnphoA-IG into a plasmid carries the IG with it; subsequently, after infection with a filamentous helper phage, ss plasmid DNA suitable for sequence analysis and useful for oligodeoxyribonucleotide-mediated mutagenesis of TnphoA-generated fusions can be isolated. The utility of TnphoA-IG was confirmed by analysis of 'blue hops' into the bla (encoding beta-lactamase) and pspE (encoding phage shock protein) genes whose products are secreted into the Escherichia coli periplasm.


Asunto(s)
Clonación Molecular/métodos , Elementos Transponibles de ADN/genética , ADN de Cadena Simple/biosíntesis , Escherichia coli/genética , Mutagénesis Insercional/genética , Fosfatasa Alcalina/genética , Bacteriófagos/genética , Secuencia de Bases , ADN de Cadena Simple/genética , Proteínas de Choque Térmico/genética , Datos de Secuencia Molecular , Secuencias Reguladoras de Ácidos Nucleicos/genética , Replicación Viral , beta-Lactamasas/genética
17.
Angew Parasitol ; 32(3): 127-32, 1991 Aug.
Artículo en Alemán | MEDLINE | ID: mdl-1928795

RESUMEN

The serum dye test by Sabin-Feldman used in the toxoplasmosis serology was adapted to the Eimeria tenella system. The antibodies detected by this specific test reflect a protective immune response. All animals immunized with virulent and attenuated Eimeria tenella oocyst antigens demonstrated serologically immune reactions. Antigen reactivities over 50% correlate with the grade of protection of animals in challenge test.


Asunto(s)
Anticuerpos Antiprotozoarios/sangre , Eimeria tenella/inmunología , Animales , Pollos , Sueros Inmunes/inmunología
18.
Angew Parasitol ; 32(2): 65-73, 1991 May.
Artículo en Alemán | MEDLINE | ID: mdl-1888074

RESUMEN

The morphologic criteria length and breadth were used for the examination of the purity from 23 strains of different Eimeria species in the fowl (Eimeria tenella, E. acervulina, E. maxima, E. necatrix) by three methods: Tabulate graphic method, determination of the size of concentration, analysis with counter. The tabulate graphic method by use of peak value as only criterion of valuation has suitable proved for the screening test. A qualification of the degree of purity is reached in the form of classes of homogeneity with the criteria peak value, first and second classes of the neighbour by the determination of the size of concentration. The quantitative certainly of the assertion increases by the analysis with counter from six statistical parameters (frequency of peak value, frequency of the sum from peak value, first and second classes of the neighbour, number of taking classes in the specific range of species, coefficient of variation, coefficient of correlation, divergence of specific index of the form in the species).


Asunto(s)
Pollos/parasitología , Coccidiosis/veterinaria , Eimeria/clasificación , Enfermedades de las Aves de Corral/parasitología , Animales , Coccidiosis/parasitología , Eimeria/aislamiento & purificación
19.
Angew Parasitol ; 32(1): 39-41, 1991 Feb.
Artículo en Alemán | MEDLINE | ID: mdl-2039092

RESUMEN

In the course of the sporogony of Eimeria tenella 7 stages were differentiated: stage of rounded sporont, stage of knob formation, first four-globe stage, pyramid-stage, second four-globe stage, stage of sporozoit differentiation, stage of complete sporulation.


Asunto(s)
Eimeria/fisiología , Animales , Ciego/parasitología , Pollos/parasitología , Coccidiosis/parasitología , Coccidiosis/veterinaria , Enfermedades de las Aves de Corral/parasitología , Esporas
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