Tratamiento del cáncer de próstata y cáncer renal con fármacos orales (abiraterona y antiangiogénicos): posicionamiento de la Asociación Española de Urología / Treatment of prostate and renal cancer with oral drugs (abiratarone and antiangiogenic agents): Positioning statement from the Spanish Association of Urology

No disponible

Sedación con sevoflurano en un procedimiento fuera del quirófano mediante el dispositivo AnaConDa ® / Sedation with sevoflurane in a procedure outside the operating theatre using the AnaConDa ® device

No disponible

Hemorragia grave de la arteria radial secundaria a punción para gasometría en un insuficiente renal en tratamiento con antiagregantes / No disponible

No disponible

Carcass characteristics of suckling lambs protected by the PGI "Lechazo de Castilla y León" European quality label: Effect of breed, sex and carcass weight.

In the Region of "Castilla y León" (Spain), as in other Mediterranean areas, fresh suckling lamb meat is regarded as having a high edible quality. The EU granted this product a Protected Geographical Indication (PGI) "Lechazo de Castilla y León" in 1999. In this study, several carcass characteristics of suckling lambs protected by this PGI were determined, while the effects of breed, sex, and carcass weight were studied. Some differences in carcass characteristics within the three PGI authorized breeds have been detected. Neither carcass weight nor conformation measurements were good predictors of the composition of the PGI protected suckling lamb carcasses. The best predictors were fatness and composition of the leg and loin tissues, although dissection of these joints is not feasible on a daily basis. Lastly, some differences, due to sex and weight were identified in conformation, fat percentage, jointing and joint composition. Differences were, however, rather small, due to the lambs' young age and low variation in carcass weight.

Changes in glycogen and trehalose content of Streptomyces brasiliensis hyphae during growth in liquid cultures under sporulating and non-sporulating conditions.

Streptomyces brasiliensis ATCC 23727 showed extensive sporulation when cultured in a liquid medium containing galactose and glutamic acid as carbon and nitrogen sources. Under such conditions, glycogen and trehalose are accumulated in the hyphae coinciding with spore formation. The results reported here suggest that glycogen accumulated in sporogenic hyphae is converted into trehalose during the final period of spore maturation. Glycogen is also accumulated in the hyphae when S. brasiliensis is cultured under conditions which did not support sporulation. Under such conditions, however, glycogen degradation is not accompanied by accumulation of trehalose. This suggest that the conversion of glycogen into trehalose might be a sporulation-specific event in streptomycetes.

Mycelial differentiation and spore formation by Streptomyces brasiliensis in submerged culture.

Streptomyces brasiliensis ATCC 23727 showed extensive sporulation when cultured in a liquid medium containing galactose and glutamic acid as carbon and nitrogen sources. Sporogenic hyphae formed under these conditions were morphologically similar and developmentally equivalent to aerial hyphae and metamorphosed into chains of spores by following a sequence of ultrastructural changes similar to that observed during growth on solid media. In addition, our electron microscopy study revealed two previously unrecognized aspects of hyphal development in streptomycetes: the formation of sporogenic hyphae was always preceded by changes in the structure of the nucleoid, and the sheath that characteristically covered these hyphae was not deposited coincidently with wall formation in the apical growing portion of the hypha.

Streptomycetes: a new model to study cell death.

Colonies of streptomycetes are now viewed as multicellular entities containing morphologically and biochemically differentiated cell types which have specific functions and precise spatial relationships to one another. Like multicellular organisms, colony development in streptomycetes is also maintained by a tight balance between cell proliferation and cell death processes. This review describes the current state of knowledge concerning cell death in streptomycetes.

Hyphal death during colony development in Streptomyces antibioticus: morphological evidence for the existence of a process of cell deletion in a multicellular prokaryote.

During the life cycle of the streptomycetes, large numbers of hyphae die; the surviving ones undergo cellular differentiation and appear as chains of spores in the mature colony. Here we report that the hyphae of Streptomyces antibioticus die through an orderly process of internal cell dismantling that permits the doomed hyphae to be eliminated with minimum disruption of the colony architecture. Morphological and biochemical approaches revealed progressive disorganization of the nucleoid substructure, followed by degradation of DNA and cytoplasmic constituents with transient maintenance of plasma membrane integrity. Then the hyphae collapsed and appeared empty of cellular contents but retained an apparently intact cell wall. In addition, hyphal death occurred at specific regions and times during colony development. Analysis of DNA degradation carried out by gel electrophoresis and studies on the presence of dying hyphae within the mycelium carried out by electron microscopy revealed two rounds of hyphal death: in the substrate mycelium during emergence of the aerial hyphae, and in the aerial mycelium during formation of the spores. This suggests that hyphal death in S. antibioticus is somehow included in the developmental program of the organism.

[Anesthesia in a patient with along-standing congenital heart malformation of the complete atrioventricular canal type for non-cardiac surgery]. / Anestesia en una paciente con malformación cardíaca congénita tipo canal auriculoventricular completo de larga evolución para cirugía no cardíaca.

Atrioventricular canal defects are a class of malformation attributable to anomalies in embryonic development of the anterior and posterior endocardial cushions. In the absence of surgical correction, death usually ensues in the first few years of life. Defects as severe as those observed in our patient are rare in adults. We describe the anesthetic management (epidural anesthesia with spontaneous ventilation by laryngeal mask) for a 46-year-old woman with this malformation who underwent emergency laparotomy.

Autoradiographic study of hyphal growth during aerial mycelium development in Streptomyces antibioticus.

The pattern of growth of aerial mycelium in Streptomyces species was investigated by autoradiography. Colonies of Streptomyces antibiotics were labeled with N-acetyl-D-[1-3H] glucosamine to localize the sites of hyphal growth during the development of aerial mycelium. Autoradiographs obtained with sections of the colonies revealed that hyphal growth occurs not only at the top of the colony but also in the inner zones of the aerial mycelium.

Synchronous germination of Streptomyces antibioticus spores: tool for the analysis of hyphal growth in liquid cultures.

We have devised a method for obtaining synchronous and dispersed growth of Streptomyces antibioticus in liquid cultures. After ultrasonic treatment, most of the spores germinated at the same time, yielding hyphae very similar in length. Dispersed growth was achieved in media without Ca2+ and in which the levels of Fe2+ and Mg2+ were carefully controlled. Studies on the kinetics of growth carried out with synchronous cultures of young hyphae revealed a multiphasic pattern of hyphal elongation, with successive periods of linear growth and changes in growth rate at defined intervals.

Growth and morphogenesis in Streptomyces.

In this report we propose a model of apical growth for streptomycetes. The apical tip is considered as a multilayered wall that expands by an inside-to-outside mechanism of growth. It is also assumed that each layer is made up of peptidoglycan blocks, each of them being the result of the biosynthetic activity of a wall-synthesizing unit or membrane-associated growth zone. According to our model, apical growth occurs as follows: as a consequence of the hydrostatic pressure and the cleavage of some bonds, the layers are pushed and forced to slide (one with respect to the other), migrating from the center of the tip (at the inside of the wall) towards a peripheral location (at the outside of the wall). The model also incorporates a mechanism by which apical growth can be regulated and coordinated with the replication of the chromosome.