Intensive care unit patients' experiences of receiving music therapy sessions during invasive procedures: A qualitative phenomenological study.

BACKGROUND: Excessive noise in intensive care units poses a significant challenge, impacting both patients and staff by elevating stress, disrupting recovery, and impeding effective communication among healthcare professionals. Despite the World Health Organization recommending noise levels below 35 dB, alarms in these units often surpass these limits, contributing to consistently high noise levels. OBJECTIVES: The aim of this study is to explore intensive care unit patients' experiences with music therapy sessions during invasive procedures. METHODS: This study was conducted using a qualitative hermeneutic phenomenological methodology grounded in Heideggerian philosophy. Interventions were conducted with a music therapist, and 14 in-depth interviews were collected. Reflexive inductive thematic analysis was performed. RESULTS/FINDINGS: From the thematic analysis extracted from the 14 personal interviews, three themes were described that represent the bulk of the experiences and emotions of the study participants following the completion of the music therapy sessions. The most noteworthy results are described in the following, organised according to each theme: (i) music therapy against noise, sounds, and light; (ii) music therapy in the face of invasive tests and techniques; and (iii) music therapy as a strategy and tool. CONCLUSIONS: Music therapy has significant potential to enhance the quality of life for patients in the intensive care unit. Music therapy can promote relaxation, reduce stress and anxiety, alleviate pain and discomfort, and improve emotional and physical wellbeing during patients' stay and invasive procedures.

Consensus document for the kangaroo mother care method.

INTRODUCTION: Currently, kangaroo mother care (KMC) is an intervention whose implementation in clinical practice varies widely. The aim of this document is to gather the latest evidence-based recommendations in an attempt to reduce interprofessional variation and increase the quality of neonatal care. METHODS: The document was developed following the guidelines provided in the Methodological Manual for the Development of Clinical Practice Guidelines of the National Health System: formulation and prioritization of clinical questions, literature search, critical reading, development of the document and external review. The target population was preterm (PT) and/or low birth weight (LBW) newborn infants admitted to a neonatal unit. RECOMMENDATIONS: Based on the current evidence, recommendations have been issued to address 18 clinical questions regarding the impact of KMC (morbidity and mortality, physiological stability, neurodevelopment, feeding, pain, families), including infants with vascular access or respiratory support devices. It also describes the KMC procedure (transfer, positioning), the facilitators and barriers related to the implementation of KMC and how to implement KMC in extremely preterm newborns (less than 28 weeks of postmenstrual age in the first days of life). CONCLUSIONS: Kangaroo mother care is a beneficial practice for PT infants, LBW infants and their families. The implementation of these recommendations may be useful in everyday clinical practice and may improve KMC outcomes and the quality of care provided to neonatal patients.

Gut Microbial Metabolome and Dysbiosis in Neurodegenerative Diseases: Psychobiotics and Fecal Microbiota Transplantation as a Therapeutic Approach-A Comprehensive Narrative Review.

The comprehensive narrative review conducted in this study delves into the mechanisms of communication and action at the molecular level in the human organism. The review addresses the complex mechanism involved in the microbiota-gut-brain axis as well as the implications of alterations in the microbial composition of patients with neurodegenerative diseases. The pathophysiology of neurodegenerative diseases with neuronal loss or death is analyzed, as well as the mechanisms of action of the main metabolites involved in the bidirectional communication through the microbiota-gut-brain axis. In addition, interventions targeting gut microbiota restructuring through fecal microbiota transplantation and the use of psychobiotics-pre- and pro-biotics-are evaluated as an opportunity to reduce the symptomatology associated with neurodegeneration in these pathologies. This review provides valuable information and facilitates a better understanding of the neurobiological mechanisms to be addressed in the treatment of neurodegenerative diseases.

Coumaphos residue transfer to honey bee brood (Apis mellifera) in realistic scenarios.

Coumaphos is a veterinary treatment administered for the control of Varroa destructor in honey bee colonies. The detection of its residues, however, has been frequently reported in beeswax. This study is pioneer to investigate whether the honey bee brood is exposed to coumaphos via contact or by ingestion of food resources due to a residue transfer inside the bee hive. This field study addresses two scenarios: 1) after its administration according to the posology using strips inside the bee hives and, 2) placing contaminated wax containing coumaphos at 10 mg/Kg into the bee hives (simulating the use of recycled wax). In bee bread, the average concentrations of residues (mean ± s.d.) were 246.66 ± 772.29 ng/g and 192.55 ± 320.19 ng/g in scenario 1 and 2, respectively. In honey, residue concentration was 1.98 ± 5.41 ng/g and 1.93 ± 6.59 ng/g. In scenario 2, exposure has led to residue detection in all larval stages at concentrations ranging from 51.93 to 383.42 ng/g (larvae), 42.20-58.54 ng/g (prepupae), 18.35-26.24 ng/g (pupae) to 21.92-35.92 ng/g (born bee). This study shows that there is a high risk for the bee brood (larvae) by ingestion of bee bread when the residue concentration is >251.31 ng/g. Residue levels in larvae or in prepupae >42.20 ng/g give rise to a moderate risk.

Acaricide residues in beeswax. Implications in honey, brood and honeybee.

For beekeeping to be sustainable, the management of colonies for the production of bee products must be economically viable without endangering the lives of bees, and must include acceptable practices such as the treatment of hives with appropriate products. Occasionally, the use of acaricides to treat the hives against varroosis is uncontrolled and can accumulate in the hives, putting the colonies at risk. In this work, a screening of seven acaricides was carried out in different apiaries in Andalusia (Spain). Their distribution in beeswax, brood, honey, and bees from colonies in different surroundings was evaluated at different times. It was found that beeswax was highly contaminated but honey, brood and bees had acceptable levels, below their respective MRL or LD50, after a certain period following varrocide treatments. Acaricides banned for their use against Varroa, such as chlorfenvinphos, cypermethrin and especially acrinathrin, were found in the hives analysed.

Differences in Expression of IQSEC2 Transcript Isoforms in Male and Female Cases with Loss of Function Variants and Neurodevelopmental Disorder.

Pathogenic hemizygous or heterozygous mutations in the IQSEC2 gene cause X-linked intellectual developmental disorder-1 (XLID1), characterized by a variable phenotype including developmental delay, intellectual disability, epilepsy, hypotonia, autism, microcephaly and stereotypies. It affects both males and females typically through loss of function in males and haploinsufficiency in heterozygous females. Females are generally less affected than males. Two novel unrelated cases, one male and one female, with de novo IQSEC2 variants were detected by trio-based whole exome sequencing. The female case had a previously undescribed frameshift mutation (NM_001111125:c.3300dup; p.Met1101Tyrfs*5), and the male showed an intronic variant in intron 6, with a previously unknown effect (NM_001111125:c.2459+21C>T). IQSEC2 gene expression study revealed that this intronic variant created an alternative donor splicing site and an aberrant product, with the inclusion of 19bp, confirming the pathogenic effect of the intron variant. Moreover, a strong reduction in the expression of the long, but also the short IQSEC2 isoforms, was detected in the male correlating with a more severe phenotype, while the female case showed no decreased expression of the short isoform, and milder effects of the disease. This suggests that the abnormal expression levels of the different IQSEC2 transcripts could be implicated in the severity of disease manifestations.

Design and Validation of a Questionnaire on Risk Perception, Coping Behaviors and Preventive Knowledge against COVID-19 among Nursing Students.

As shown in the previous literature, in view of the future responsibilities of nursing professionals and the consequences for healthcare, it is of great interest to examine their risk perceptions, coping behaviors, and sense of coherency during the COVID-19 pandemic. The purpose of this study is to design and to validate a specific questionnaire that evaluates the factors relating to perceived risk, coping behaviors, and preventive knowledge against COVID-19 infection among nursing students from Spain. This is a psychometric study of a questionnaire's design and its validation in 1562 nursing students at 16 undergraduate nursing institutions in Spain. An ad-hoc survey was designed by a panel of six experts drawing from the literature. After a trial test, the questionnaire was formed with four scales (perception, risk, coping, and knowledge of preventive practices for COVID-19), with a total of 69 items. The final questionnaire was composed of 52 items grouped into four scales, with good psychometric properties to measure risk perception (Cronbach's alpha 0.735), factors related to perceived risk (Cronbach's alpha 0.653), coping behaviors (Cronbach's alpha 0.80), and knowledge of preventive practices against COVID-19 (Cronbach's alpha 0.77). This questionnaire, specifically designed and validated for nursing students, is the first to address four important areas in the development of preventive measures against COVID-19.

Digitized Prenatal Newsletter: Impact on Obstetric Patient Satisfaction and Loyalty.

The high demand for health information from pregnant women has encouraged the creation of an informative program through a weekly digital newsletter. The objective of this study is to evaluate its quality as a digital communication medium, in terms of satisfaction and loyalty to the pregnancy follow-up and delivery service. A cross-sectional, prospective study was carried out, surveying 179 patients by means of an online self-referral questionnaire including variables related to humanization, information needs, perceived accompaniment and satisfaction, as well as factors related to its influence on their decision to remain loyal to the center. A total of 81.2% of the participants showed high levels of satisfaction with the program. Satisfaction among nulliparous patients was significantly lower in several aspects. The resolution of doubts and the perception of peace of mind following the information received was positive for 54.8%. Of the patients in the program, 88.8% finally remained at the center, showing a strong influence of the program on their decision (mean value 75 on 1 to 100 scale). A weekly digital newsletter with specific information reduced the demand for information from pregnant women, generating high levels of satisfaction and positively influencing the decision to remain loyal to the Center.

Percepciones de los estudiantes de enfermería sobre el uso del portafolio en la integración teórico-práctica / Nursing students' perceptions on the use of the portfolio in theoretical-practical integration

Objetivo: explorar las percepciones de los estudiantes sobre el uso del portafolio y su potencial para integrar la teoría y la práctica. Metodología: estudio descriptivo e interpretativo con un enfoque cualitativo. Se realizó analizando los datos recopilados de los portafolios y cuestionarios de los estudiantes de enfermería de cuarto año, haciendo prácticas durante el año 2014-2015. Resultados: las percepciones de los estudiantes corroboran que el portafolio permite establecer un vínculo entre la teoría y la práctica, así como poner en práctica lo aprendido. Conclusiones: El análisis de los portafolios ha revelado que la teoría y la práctica se retroalimentan mutuamente, lo que hace que los estudiantes tengan el papel principal. El componente reflexivo ha contribuido a reducir el llamado choque de la realidad, permitiendo una transferencia de teoría a una práctica dinámica. (AU)

Objective: exploring the students' perceptions of the use of the portfolio, and its potential to integrate theory and practice. Methods: To illustrate that, a descriptive and interpretative study was carried out with a qualitative approach. The study was carried out analysing the data collected from the portfolios and questionnaires from the 4th year nursing students, doing practicum during the 2014-2015 academic year. Results: The students' perceptions corroborate that the portfolio allows to establish a link between theory and practice, as well as to put into practice what has been learned. Conclusions: The analysis of portfolios has revealed that theory and practice mutually feedback each other, having the students the leading role in that. The reflective component has contributed to reduce the so-called reality shock, allowing a transfer of theory into a fully dynamic practice. (AU)

Lateral Kangaroo Care in Hemodynamic Stability of Extremely Preterm Infants: Protocol Study for a Non-Inferiority Randomized Controlled Trial CANGULAT.

INTRODUCTION: This study aims to assess the efficacy of the modified kangaroo care lateral position on the thermal stability of preterm neonates versus conventional kangaroo care prone position. MATERIAL AND METHODS: A non-inferiority randomized parallel clinical trial. Kangaroo care will be performed in a lateral position for the experimental group and in a prone position for the control group preterm. The study will take place at the neonatal intensive care unit (NICU) of a University Hospital. The participants will be extremely premature infants (under 28 weeks of gestational age) along the first five days of life, hemodynamically stable, with mother or father willing to do kangaroo care and give their written consent to participate in the study. The sample size calculated was 35 participants in each group. When the premature infant is hemodynamically stable and one of the parents stays in the NICU, the patient will be randomized into two groups: an experimental group or a control group. The primary outcome is premature infant axillary temperature. Neonatal pain level and intraventricular hemorrhage are secondary outcomes. DISCUSSION: There is no scientific evidence on modified kangaroo care lateral position. Furthermore, there is little evidence of increased intraventricular hemorrhage association with the lateral head position necessary in conventional or prone kangaroo care in extremely premature newborns. Kangaroo care is a priority intervention in neonatal units increasing the time of use more and more, making postural changes necessary to optimize comfort and minimize risks with kangaroo care lateral position as an alternative to conventional prone position kangaroo care. Meanwhile, it is essential to ensure that the conventional kangaroo care prone position, which requires the head to lay sideways, is a safe position in terms of preventing intraventricular hemorrhage in the first five days of life of children under 28 weeks of gestational age. Trial registration at clinicaltrials.gov: NCT03990116.

Universal scaling laws rule explosive growth in human cancers.

Most physical and other natural systems are complex entities composed of a large number of interacting individual elements. It is a surprising fact that they often obey the so-called scaling laws relating an observable quantity with a measure of the size of the system. Here we describe the discovery of universal superlinear metabolic scaling laws in human cancers. This dependence underpins increasing tumour aggressiveness, due to evolutionary dynamics, which leads to an explosive growth as the disease progresses. We validated this dynamic using longitudinal volumetric data of different histologies from large cohorts of cancer patients. To explain our observations we put forward increasingly-complex biologically-inspired mathematical models that captured the key processes governing tumor growth. Our models predicted that the emergence of superlinear allometric scaling laws is an inherently three-dimensional phenomenon. Moreover, the scaling laws thereby identified allowed us to define a set of metabolic metrics with prognostic value, thus providing added clinical utility to the base findings.

Analysis of Multiclass Antibiotics in Lettuce by Liquid Chromatography-Tandem Mass Spectrometry to Monitor Their Plant Uptake.

The main entry routes of antibiotics in the environment are the application of organic wastes to improve soil quality and the irrigation with recycled water. Once in the environment, antibiotics can be introduced in the food chain through their uptake by crops. This paper describes the development of an analytical method based on ultrasound-assisted extraction for the determination of seven antibiotics in lettuce. The developed method was applied to evaluate antibiotic uptake by lettuce grown in pots fertilized with composted poultry litter doped with a mixture of antibiotics to reach a final concentration of 2.5 µg/g in soil. Lettuce were harvested after 21, 36, and 55 days. Five of the seven studied antibiotics were found in all samples. The highest uptake was found for lincomycin (51 ng/g fresh weight) followed by sulfamethoxazole (44 ng/g fresh weight) and sulfamethazine (21 ng/g fresh weight) in lettuce harvested after 21 days. An important decrease of their levels was observed after 36 days, but these levels remained similar after 55 days. Although levels found in lettuce were low, the presence of antibiotics demonstrates the need for further assessing food safety risks related with the use of soil amendments or irrigation water contaminated with antibiotics.

Rapid determination of antibiotic residues in cereals by liquid chromatography triple mass spectrometry.

Antibiotics may be present in agricultural soils through the application of organic amendments as fertilizers or by irrigation of fields with recycled water. As a result of these agricultural practices, antibiotics in soil can lead to their uptake by plants, entering in this way the food chain. Studies on the levels of antibiotics in cereal samples are scarce in the available literature. In this work, an analytical method was developed for the determination of 19 antibiotics (fluoroquinolones, sulfonamides, tetracyclines, and lincosamides) in four types of cereal grains (wheat, barley, rice, and oat). Ultrasound-assisted matrix solid-phase dispersion was selected as extraction technique with recoveries of target analytes ranging from 73 to 127% for the four cereals analyzed. Limits of quantification obtained ranged from 0.8 to 5.8 ng g-1. Compared with methods described for the analysis of antibiotics in cereals, the developed method uses a lower volume of extraction solvent and very good recoveries were obtained for all compounds. The validated method was applied to the analysis of different types of cereals samples, harvested from agricultural fields or purchased from local supermarkets. The analysis of the five cereal samples grown in fields with 3 years of consecutive organic amendments revealed that none of the nineteen antibiotics selected were found in any sample. Eleven commercial samples of cereals of different types and presentations were analyzed and enrofloxacin was detected in one rice sample; the presence of enrofloxacin in cereals or its incorporation into crops from soil or water not previously reported. Graphical abstract.

Persistence and availability of veterinary antibiotics in soil and soil-manure systems.

The availability and persistence of various antibiotics in soil and soil amended with composted poultry manure were investigated through laboratory incubation assays. Six veterinary antibiotics (one fluoroquinolone, two tetracyclines, two sulfonamides and one lincosamide) and one active metabolite (ciprofloxacin) were studied. The incubation assays were conducted at a controlled temperature of 25 °C with different water regimes, such as constant moisture content (80% of water holding capacity) and drying-rewetting cycles. The studied antibiotics were determined in soil and soil aqueous phase samples by LC-MS/MS using internal standards. The results indicated that the highest levels found in the soil aqueous phase were for sulfamethoxazole, followed by sulfamethazine and lincomycin, being very low the levels of chlortetracycline, doxycycline, ciprofloxacin and enrofloxacin (≤1.8%). A positive correlation was observed between the antibiotic concentrations and the content of the dissolved organic carbon in soil aqueous phase with the incubation time. An increase in the apparent sorption coefficients of these antibiotics, except chlortetracycline and lincomycin, was observed when the soil was amended with composted manure. Except for fluoroquinolones, with remaining residues around 70% after 90 days of incubation, a fast dissipation of antibiotics was observed during the assay, with half-lives ranging from 8 to 27 days. These values increased between 6% and 53% in manure amended soil; nevertheless, half-lives remained short (9 days and 27 days for lincomycin and sulfamethazine, respectively). Similar results were obtained with soil under drying-rewetting cycles showing somewhat lower values in soil aqueous phase and slightly shorter half-lives in some cases. The results obtained pointed out that the route of entry of antibiotics into the soil, through recycled water or manure, may have an important effect on their behavior, particularly regarding their availability in soil.

Impacto de la evaluación de competencias en la calidad del aprendizaje: percepción de discentes y docentes de Grado en Enfermería / Competency assessment impact in quality of learning: nursing degree learner's and teacher's perception

Introducción: El enfoque de educación basada en competencias pretende reducir la brecha entre educación y empleo buscando lograr un aprendizaje profundo, significativo, para toda la vida, transferible a situaciones reales, complejas y cambiantes. Conceptualizar la evaluación a partir de este enfoque implica asumir su carácter multidimensional diseñando una evaluación para el aprendizaje y no sólo del aprendizaje, en la búsqueda de formas de evaluación capaces de mejorar la calidad del mismo. La conciencia del impacto de la evaluación en el aprendizaje obliga a plantear la evaluación como un proceso compartido que sea simultáneamente causa y efecto de los aprendizajes. En este contexto, el objetivo de este estudio es conocer la percepción de discentes y docentes en relación a las prácticas actuales de evaluación de competencias y su impacto en la calidad del aprendizaje. Metodología: Estudio descriptivo interpretativo. Análisis cualitativo de los datos recogidos mediante cuestionarios abiertos y grupos de discusión de discentes y docentes del Grado en Enfermería. Resultados: Ambos grupos consideran que las prácticas actuales de evaluación de competencias condicionan el aprendizaje, sin embargo, existen grandes diferencias en sus percepciones. Los docentes perciben este impacto como negativo y afirman que para los estudiantes la evaluación consiste sólo en superar asignaturas. Los estudiantes manifiestan que la evaluación les influye positivamente al orientar su aprendizaje y ofrecerles oportunidades de mejora. Conclusiones: Los docentes perciben dificultades para superar la orientación tradicional de la evaluación, mientras los estudiantes perciben su función más formativa y demandan para ella un feedback suficiente y de calidad

Introduction: Competency based education offers the promise of reducing the gap between education and employment and concerns in Higher Education have revolved around how to achieve deep and meaningful learning, a long life learning, so that learning transfer to real situations, complex and changing, would be possible. Conceptualizing assessment from competency based education approach requires assuming its multidimensional character and designing an assessment for learning and not only assessment of learning seeking to improve learning quality. Awareness of the assessment impact on learning requires raising evaluation as a shared process able to be simultaneously cause and effect of learning. In this context, the aim of this study is to determine learners and teachers perceptions about current practices of competence assessment and its impact on the quality of learning. Methodology: Interpretative descriptive study. Qualitative analysis of data collected through open questionnaires and discussion groups with Degree in Nursing learners and teachers. Results: Both teachers and learners believe that current assessment practices determine learning skills, but there are big differences in their perceptions. Teachers perceive negatively this impact and claim that for student’s evaluation is only overcoming subjects while students state that assessment influences them positively, guiding their learning and offering improvement opportunities. Conclusions: teachers perceive difficulties in overcoming evaluation`s traditional, while students perceive its most formative function and demand for it sufficient and quality feedback

Analysis of emerging organic contaminants in poultry manure by gas chromatography-tandem mass spectrometry.

A multiresidue method was developed for the determination of 19 emerging organic contaminants (pharmaceutical drugs, personal care products, and bisphenol A) in poultry manure. Lyophilized samples of manure were extracted by ultrasound-assisted matrix solid-phase dispersion and the extracts were analyzed by gas chromatography with tandem mass spectrometry after derivatization. Analysis of spiked poultry manure samples, at levels ranging from 25 to 150 ng/g, gave satisfactory recovery results for all the compounds, with values from 67 to 106%. The developed procedure provided detection limits that ranged from 0.9 to 2.2 ng/g. Finally, the validated method was applied to poultry manure samples collected from 23 poultry farms in Spain. Salicylic acid was found in most of the samples analyzed at levels up to 2501 ng/g, whereas, methyl paraben, orthophenylphenol, ibuprofen, paracetamol, and carbamazepine were detected at levels up to 250 ng/g. Composting of manure showed an important decrease in the levels of the detected contaminants.

Percepciones de los estudiantes sobre el desarrollo del pensamiento crítico-reflexivo a través del portafolio de prácticas / Perceptions of Students about the Development of Critical-Reflective Thinking through the Portfolio of Practices / Percepções dos alunos sobre o desenvolvimento do pensamento crítico-reflexivo através da sua carteira de práticas

Introducción: la formación y adquisición de pensamiento crítico-reflexivo en los estudiantes de enfermería es fundamental para formar profesionales reflexivos, donde las intervenciones y acciones son resultado de la interrogación constante y del pensamiento crítico, para adquirir el principio de aprender a aprender. Uno de los métodos que han surgido para fomentar cualquier modalidad de pensamiento crítico-reflexivo en los estudiantes es el portafolio. Objetivo: conocer las percepciones de los estudiantes sobre el desarrollo del pensamiento crítico-reflexivo a través del portafolio de prácticas. Material y métodos: estudio descriptivo cualitativo. La recolección de los datos se realizó a través de los portafolios y los cuestionarios de los estudiantes de Enfermería de cuarto curso de la Escuela Universitaria de Enfermería Cruz Roja, que realizaron prácticas durante 2013-2014. Los resultados se examinaron con el análisis de contenido. Resultados: los estudiantes consideran que el pensamiento crítico generado al realizar el portafolio les ayuda principalmente para el razonamiento reflexivo en la acción y también a integrar el conocimiento. Conclusiones: los estudiantes perciben que el pensamiento crítico-reflexivo constituye un instrumento esencial en el proceso de superación de contradicciones que se encuentran presentes en los procesos de enseñanza-aprendizaje durante sus prácticas clínicas.

Introduction: Training and acquisition of critical-reflective thinking in nursing students is essential to form reflective practitioners, where interventions and actions are the result of constant questioning and critical thinking, to acquire the principle of learning to learn. One of the methods that have emerged to encourage any form of reflective critical thinking in students is the portfolio. Objective: To know the perceptions of students about the development of critical-reflective thinking through the portfolio of practices. Material and Methods: A qualitative descriptive study. Data collection was conducted through questionnaires portfolios and nursing students of 4th year of the School of Nursing Red Cross, who conducted practices during course 2013-2014. The results were analyzed using content analysis. Results: Students consider that critical thinking generated when performing portfolio, helps them mainly for reflective thinking in action and also to integrate knowledge. Conclusions: Students perceive that the critical-reflective thinking is an essential tool in the process of overcoming contradictions that are present in the teaching and learning processes during their clinical practice.

Introdução: formação e aquisição de pensamento crítico-reflexivo em estudantes de enfermagem é essencial para formar profissionais reflexivos, onde as intervenções e ações são o resultado da constante questionamento e pensamento crítico, para adquirir o princípio de aprender a aprender. Um dos métodos que surgiram para encorajar qualquer forma de pensamento crítico reflexivo em estudantes é a carteira. Objetivo: conhecer a percepção dos estudantes sobre o desenvolvimento do pensamento crítico-reflexivo através da sua carteira de práticas. Material e Métodos: estudo descritivo, qualitativo. A coleta de dados foi realizada por meio de questionários carteiras e estudantes de enfermagem de 4o ano da Escola de Enfermagem da Cruz Vermelha, que conduziu práticas durante o curso 2013-2014. Os resultados foram analisados por meio de análise de conteúdo. Resultados: Os alunos consideram que o pensamento crítico gerado durante a execução da carteira, ajuda-os principalmente para o aprofundamento da reflexão em ação e também para integrar o conhecimento. Conclusões: os alunos percebem que o pensamento crítico-reflexivo é uma ferramenta essencial no processo de superação das contradições que estão presentes em processos de ensino e aprendizagem durante a sua prática clínica.

Simultaneous determination of multiclass emerging contaminants in aquatic plants by ultrasound-assisted matrix solid-phase dispersion and GC-MS.

A multiresidue method was developed for the simultaneous determination of 31 emerging contaminants (pharmaceutical compounds, hormones, personal care products, biocides, and flame retardants) in aquatic plants. Analytes were extracted by ultrasound-assisted matrix solid-phase dispersion (UA-MSPD) and determined by gas chromatography-mass spectrometry after sylilation, The method was validated for different aquatic plants (Typha angustifolia, Arundo donax, and Lemna minor) and a semiaquatic cultivated plant (Oryza sativa) with good recoveries at concentrations of 100 and 25 ng g-1 wet weight, ranging from 70 to 120 %, and low method detection limits (0.3 to 2.2 ng g-1 wet weight). A significant difference of the chromatographic response was observed for some compounds in neat solvent versus matrix extracts, and therefore, quantification was carried out using matrix-matched standards in order to overcome this matrix effect. Aquatic plants taken from rivers located at three Spanish regions were analyzed, and the compounds detected were parabens, bisphenol A, benzophenone-3, cyfluthrin, and cypermethrin. The levels found ranged from 6 to 25 ng g-1 wet weight except for cypermethrin that was detected at 235 ng g-1 wet weight in O. sativa samples.

Application of matrix solid-phase dispersion followed by GC-MS/MS to the analysis of emerging contaminants in vegetables.

A multiresidue method for the determination of 17 emerging contaminants in vegetables was developed based on ultrasound-assisted matrix solid-phase dispersion (MSPD). The analysis was performed using isotope dilution gas chromatography tandem mass spectrometry. In the development of the MSPD procedure, different parameters such as sonication and the type of sorbent or extraction solvent were assayed. Manual and in situ derivatization was assayed and the chromatographic response was higher when the reaction takes place in the injection port. The limits of detection obtained for the studied compounds were in the range of 0.1-0.4ngg(-1) for the different vegetables analyzed. The developed method was applied to vegetables obtained from several local markets. At least one of the organophosphates was detected in the analyzed samples at levels ranging from 0.6 to 4.6ngg(-1) and bisphenol A was detected in all the samples at concentration up to 16ngg(-1).

El estudiante como protagonista de su aprendizaje: La necesidad del uso del portafolio en enfermería dentro del contexto de Educación Superior / The student as protagonists of their learning: the need to use the portfolio in nursing within the context of higher education

Las reformas promovidas por el proceso Bolonia han implicado cambios en las formas de enseñar y aprender dentro del marco del Espacio Europeo de Educación Superior. Esta amplia remodelación trae consigo por un lado un cambio estructural y, por otro, una reconceptualización del proceso de aprendizaje apoyada en las teorías constructivistas, a través de estrategias metodológicas de construcción del conocimiento desarrolladas por los estudiantes. El objetivo del presente artículo es presentar una reflexión teórica acerca del uso del portafolio como recurso didáctico para la enseñanza y aprendizaje dentro de este contexto. Se ha observado que la implicación de los estudiantes en la identificación de los problemas a través del portafolio, facilita a los docentes una mayor comprensión de los procesos de aprendizaje de los estudiantes, así como una oportunidad para éstos de efectuar un aprendizaje más reflexivo, autónomo y participativo, tan importante en disciplinas eminentemente prácticas como la Enfermería

The reforms promoted by the Bologna process have led to changes in the forms of teaching and learning within the framework of European Higher Education. This brings extensive remodeling on the one hand a structural change and on the other, a re-conceptualization of the learning process supported by constructivist theories, through methodological strategies developed knowledge construction by students. The aim of this paper is to present a theoretical reflection on using the portfolio as a teaching resource for teaching and learning in this context. It has been observed that the involvement of students in identifying problems through the portfolio, provides teachers with a greater understanding of the learning processes of students as well as an opportunity for them to make a more reflective learning, independent and participatory practices eminently important in disciplines such as Nursing