RESUMEN
PM1 and PM2.5 aerosol samples collected during four seasons were analysed for bioaccessibility of 21 elements and oxidative potential (OP) determined by the dithiothreitol (DTT) assay in three simulated lung fluids (SLFs): deionised water, simulated alveoli fluid and Gamble's solution. Most elements had higher bioaccessibility in the submicron fraction than in the fine size fraction. The bioaccessibility of the element not only depends on the aerosol size fraction, but also varies between the three SLFs. In addition, the bioaccessibility of elements depends on both their chemical compound and the composition of the SLF. A very high bioaccessibility (up to 98%) was observed for As, Sb and Cd in all studied SLFs. The lowest bioaccessibility was observed for Ti, Al and Fe. The OP of urban particulate matter (PM), was studied as a relevant metric for health effects. The difference of OP value in simulated alveoli fluid and Gamble's solution compared to deionised water indicate the crucial effect of individual SLFs' composition on the OP. The complexation of elements with different ligands present in the solution can influence OPDTT depletion and, therefore, the potential health effects of inhaled aerosol. The correlation coefficients between total or bioaccessible concentrations of studied elements and volume normalised OP were calculated to examine the relationship between the elements and the OP. The strong positive correlations between some elements (i.e. Cd, Pb, As, Zn, Sn, Cu, Co, Ni, Mn) and DTT activity suggest their participation in the oxidative activity of PM.
Asunto(s)
Contaminantes Atmosféricos , Monitoreo del Ambiente , Aerosoles , Contaminantes Atmosféricos/análisis , Pulmón , Estrés Oxidativo , Material Particulado/análisisRESUMEN
Lead nanoparticles (NPs) are released into air from metal processing, road transport or combustion processes. Inhalation exposure is therefore very likely to occur. However, even though the effects of bulk lead are well known, there is limited knowledge regarding impact of Pb NPs inhalation. This study focused on acute and subchronic exposures to lead oxide nanoparticles (PbO NPs). Mice were exposed to PbO NPs in whole body inhalation chambers for 4-72 h in acute experiment (4.05 × 106 PbO NPs/cm3), and for 1-11 weeks in subchronic experiment (3.83 × 105 particles/cm3 in lower and 1.93 × 106 particles/cm3 in higher exposure group). Presence of NPs was confirmed in all studied organs, including brain, which is very important considering lead neurotoxicity. Lead concentration gradually increased in all tissues depending on the exposure concentration and duration. The most burdened organs were lung and kidney, however liver and brain also showed significant increase of lead concentration during exposure. Histological analysis documented numerous morphological alterations and tissue damage, mainly in lung, but also in liver. Mild pathological changes were observed also in kidney and brain. Levels of glutathione (reduced and oxidized) were modulated mainly in lung in both, acute and subchronic exposures. Increase of lipid peroxidation was observed in kidney after acute exposure. This study characterized impacts of short to longer-term inhalation exposure, proved transport of PbO NPs to secondary organs, documented time and concentration dependent gradual increase of Pb concentration and histopathological damage in tissues.
Asunto(s)
Exposición por Inhalación/efectos adversos , Plomo/farmacocinética , Plomo/toxicidad , Peroxidación de Lípido/efectos de los fármacos , Nanopartículas/administración & dosificación , Nanopartículas/toxicidad , Óxidos/farmacocinética , Óxidos/toxicidad , Administración por Inhalación , Animales , Encéfalo/efectos de los fármacos , Glutatión/metabolismo , Riñón/efectos de los fármacos , Plomo/administración & dosificación , Plomo/química , Hígado/efectos de los fármacos , Pulmón/efectos de los fármacos , Masculino , Ratones , Nanopartículas/química , Óxidos/administración & dosificación , Óxidos/química , Distribución TisularRESUMEN
BACKGROUND: Lead is well known environmental pollutant, which can cause toxic effects in multiple organ systems. However, the influence of lead oxide nanoparticles, frequently emitted to the environment by high temperature technological processes, is still concealed. Therefore, we investigate lead oxide nanoparticle distribution through the body upon their entry into lungs and determine the microscopic and ultramicroscopic changes caused by the nanoparticles in primary and secondary target organs. METHODS: Adult female mice (ICR strain) were continuously exposed to lead oxide nanoparticles (PbO-NPs) with an average concentration approximately 106 particles/cm3 for 6 weeks (24 h/day, 7 days/week). At the end of the exposure period, lung, brain, liver, kidney, spleen, and blood were collected for chemical, histological, immunohistochemical and electron microscopic analyses. RESULTS: Lead content was found to be the highest in the kidney and lungs, followed by the liver and spleen; the smallest content of lead was found in brain. Nanoparticles were located in all analysed tissues and their highest number was found in the lung and liver. Kidney, spleen and brain contained lower number of nanoparticles, being about the same in all three organs. Lungs of animals exposed to lead oxide nanoparticles exhibited hyperaemia, small areas of atelectasis, alveolar emphysema, focal acute catarrhal bronchiolitis and also haemostasis with presence of siderophages in some animals. Nanoparticles were located in phagosomes or formed clusters within cytoplasmic vesicles. In the liver, lead oxide nanoparticle exposure caused hepatic remodeling with enlargement and hydropic degeneration of hepatocytes, centrilobular hypertrophy of hepatocytes with karyomegaly, areas of hepatic necrosis, occasional periportal inflammation, and extensive accumulation of lipid droplets. Nanoparticles were accumulated within mitochondria and peroxisomes forming aggregates enveloped by an electron-dense mitochondrial matrix. Only in some kidney samples, we observed areas of inflammatory infiltrates around renal corpuscles, tubules or vessels in the cortex. Lead oxide nanoparticles were dispersed in the cytoplasm, but not within cell organelles. There were no significant morphological changes in the spleen as a secondary target organ. Thus, pathological changes correlated with the amount of nanoparticles found in cells rather than with the concentration of lead in a given organ. CONCLUSIONS: Sub-chronic exposure to lead oxide nanoparticles has profound negative effects at both cellular and tissue levels. Notably, the fate and arrangement of lead oxide nanoparticles were dependent on the type of organs.
Asunto(s)
Contaminantes Ambientales/farmacocinética , Plomo/farmacocinética , Pulmón/metabolismo , Nanopartículas del Metal , Óxidos/farmacocinética , Animales , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Encéfalo/ultraestructura , Contaminantes Ambientales/administración & dosificación , Contaminantes Ambientales/química , Contaminantes Ambientales/toxicidad , Femenino , Exposición por Inhalación , Riñón/efectos de los fármacos , Riñón/metabolismo , Riñón/ultraestructura , Plomo/administración & dosificación , Plomo/química , Plomo/toxicidad , Hígado/efectos de los fármacos , Hígado/metabolismo , Hígado/ultraestructura , Pulmón/efectos de los fármacos , Pulmón/ultraestructura , Nanopartículas del Metal/administración & dosificación , Nanopartículas del Metal/química , Nanopartículas del Metal/toxicidad , Ratones Endogámicos ICR , Óxidos/administración & dosificación , Óxidos/química , Óxidos/toxicidad , Medición de Riesgo , Bazo/efectos de los fármacos , Bazo/metabolismo , Bazo/ultraestructura , Distribución Tisular , ToxicocinéticaRESUMEN
Cadmium nanoparticles can represent a risk in both industrial and environmental settings, but there is little knowledge on the impacts of their inhalation, especially concerning longer-term exposures. In this study, mice were exposed to cadmium oxide (CdO) nanoparticles in whole body inhalation chambers for 4 to 72 h in acute and 1 to 13 weeks (24 h/day, 7 days/week) in chronic exposure to investigate the dynamics of nanoparticle uptake and effects. In the acute experiment, mice were exposed to 2.95 × 106 particles/cm3 (31.7 µg CdO/m3). The same concentration and a lower one (1.18 × 106 particles/cm3, 12.7 µg CdO/m3) were used for the chronic exposure. Transmission electron microscopy documented distribution of nanoparticles into all studied organs. Major portion of nanoparticles was retained in the lung, but longer exposure led to a greater relative redistribution into secondary organs, namely the kidney, and also the liver and spleen. Accumulation of Cd in the lung and liver occurred already after 24 h and in the brain, kidney, and spleen after 72 h of exposure, and a further increase of Cd levels was observed throughout the chronic exposure. There were significant differences in both Cd accumulation and effects between the two exposure doses. Lung weight in the higher exposure group increased up to 2-fold compared to the control. Histological analyses showed dose-dependent alterations in lung and liver morphology and damage to their tissue. Modulation of oxidative stress parameters including glutathione levels and increased lipid peroxidation occurred mainly after the greater chronic exposure. The results emphasize risk of longer-term inhalation of cadmium nanoparticles, since adverse effects occurring after shorter exposures gradually progressed with a longer exposure duration.