1.
Braz J Microbiol
; 46(1): 113-6, 2015 Mar.
Artículo
en Inglés
| MEDLINE
| ID: mdl-26221095
RESUMEN
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
Asunto(s)
Aminohidrolasas/análisis , Colorimetría/métodos , Ensayos Analíticos de Alto Rendimiento/métodos , Hidroliasas/análisis , Amidohidrolasas/antagonistas & inhibidores , Concentración de Iones de Hidrógeno
2.
Braz. j. microbiol
; 46(1): 113-116, 05/2015. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-748237
RESUMEN
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.