Metal mixture exposures and multiplexed autoantibody screening in Navajo communities exposed to uranium mine wastes.

Background: Environmental exposures to metals in uranium mining wastes and drinking water were documented in more than half of the 1304 Navajo community members of the Diné Network for Environmental Health (DiNEH) Project, the first comprehensive assessment of exposures to these metals and community health on the Navajo Nation. Objective: Evaluate environmental exposures among participants who provided blood and urine samples using multiplexed autoantibody positivity as an early effect biomarker. Methods: Survey and geospatial location data, well water quality, and metals biomonitoring were used to assess exposures to mixed-metal wastes from 100 abandoned uranium waste sites. Results: We observed that the prevalence of multiplexed autoantibody positivity in 239 participants was more than double that reported for the U.S. population (27.2% v. 13.8%) even though the national prevalence was generated using a different assay, the HEp-2 cell-based antinuclear antibody test. Increased risk of multiplexed autoantibody screening positivity (OR = 3.07,95%CI 1.15-8.22) was found among DiNEH study people who lived close to uranium mine and milling wastes and consumed metals in drinking water. Associations for females were even stronger when they lived closed to contaminated uranium mining and milling sites. Anti-U1-RNP antibodies were associated with water consumption of nickel. Conclusion: Proximity to waste sites and consumption of metals in water even below current drinking water standards were associated with perturbations of immune tolerance. These findings are consistent with previous studies of autoimmunity in the local population and demonstrate that multiplexed autoantibody screening method has a potential as sentinel indicator of exposures to environmental metals. Impact statement: This is the first, community-engaged environmental health study in exposed Navajo communities that applied clinical multiplexed testing in risk assessment of environmental metals associated with abandoned, unremediated uranium mining and milling waste sites. Routine clinical autoimmunity measures could be used as early effect biomarkers of environmental metal exposures.

Cross-cultural applicability of eye-tracking in assessing attention to emotional faces in preschool-aged children.

Humans show an attention bias toward emotional versus neutral information, which is considered an adaptive pattern of information processing. Deviations from this pattern have been observed in children with socially withdrawn behaviors, with most research being conducted in controlled settings among children from urban areas. The goal of the current study was to examine the cross-cultural applicability of two eye-tracking-based measures in assessing attention biases and their relations to children's symptoms of socially withdrawn behaviors in two independent and diverse samples of preschool children. The cross-cultural comparison was conducted between the Navajo Birth Cohort study (NBCS), an indigenous cohort with relatively low socioeconomic status (SES), and the Illinois Kids Development study (IKIDS), a primarily Non-Hispanic White and high SES cohort. Children in both cohorts completed eye-tracking tasks with pictures of emotional faces, and mothers reported on children's symptoms of socially withdrawn behaviors. Results showed that general patterns of attention biases were mostly the same across samples, reflecting heightened attention toward emotional versus neutral faces. The differences across two samples mostly involved the magnitude of attention biases. NBCS children were slower to disengage from happy faces when these emotional faces were paired with neutral faces. Additionally, socially withdrawn children in the NBCS sample showed a pattern of attentional avoidance for emotional faces. The comparability of overall patterns of attention biases provides initial support for the cross-cultural applicability of the eye-tracking measures and demonstrates the robustness of these methods across clinical and community settings. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Environmental uranium exposures and cytokine profiles among mother-newborn baby pairs from the Navajo Βirth Cohort Study.

The Navajo Nation was heavily mined for uranium (U) during the cold-war leading to a legacy of >1100 abandoned U mining, milling and associated waste sites. The Navajo Birth Cohort Study was initiated to assess the effect of non-occupational legacy exposure to U during pregnancy on birth outcomes and child development. We report that 92% of babies with detectable urine U at birth were born from mothers who had urine U concentrations greater than national norms during pregnancy, indicative of prenatal exposure to U. To assess immune alterations associated with U exposure on both mothers and babies, we investigated associations between cytokine profiles and maternal U and associations of these measures with cytokine profiles in babies. Effect sizes for the differences in cytokine profiles were more evident among babies than mothers. Overall, there were seven cytokines (IFN-γ, IL-1ß, IL-2, IL-4, IL-10, GM-CSF, and TNF-α), for which the effect size for babies with higher than the national U concentrations was medium to large (ORs of 2.21 (1.08-4.52) through 1.71(0.76-3.83). In contrast, only three cytokines (IL-8, IL-12p70, and TNF-α) had effect sizes which almost reached medium strength (ORs of 1.64 (0.74-4.05) through 1.36 (0.65-2.87) in mothers with U above national norms. The effects of prenatal exposures to uranium and associated alterations in systemic immune responses resulting from U exposure could impact both maternal health as well as healthy child development through induction of inflammation, autoimmunity or other chronic diseases related to immune dysfunction that may affect long-term health.

Efficacy of hypochlorous acid (HOCl) fog in sanitizing surfaces against Enterococcus faecalis.

BACKGROUND: Fogging is an efficient method when disinfection of large areas is desired. METHODS: Two methods of ultrasonic fogging, pulsed and continuous, were compared on bacteria dried on either aluminum or polystyrene surfaces. We characterized commercial and home-made hypochlorous acid (HOCl) with respect to storage and means of production. RESULTS: We found that the initial chlorine concentration of the commercial solution was approximately 550 ppm, and when stored open under ambient conditions, the chlorine content decreased at a rate of 30% every 100 days. The HOCl produced using the home synthesizers had a maximum chlorine content of 257.6 ppm which decayed by 65% after 100 days. A second synthesizer produced a liquid with high chlorine content and pH, 750ppm and pH = 8.55. The anti-bacterial efficacy was probed using Enterococcus faecalis, a persistent source of infection in public and clinical spaces. Time course studies determined that E. faecalis could survive dry on surfaces for more than 12 weeks, but was easily eliminated in half the fogging time. CONCLUSIONS: The most effective mode of application was determined to be continuous fogging where a 6.59 log reduction was established in vertical geometry. The optimal pulsed fogging protocol produced a similar reduction, but required nearly 5 times as long. The home synthesized versions yielded much lower log bacterial reductions. No significant differences in outcome were determined between polymer or metal surfaces.

Marginal probabilities and point estimation for conditionally specified logistic regression.

Conditionally specified logistic regression (CSLR) models p binary response variables. It is shown that marginal probabilities can be derived for a CSLR model. We also extend the CSLR model by allowing third order interactions. We apply two versions of CSLR to simulated data and a set of real data, and compare the results to those from other modeling methods.

Active smoking, secondhand smoke exposure and serum cotinine levels among Cheyenne River Sioux communities in context of a Tribal Public Health Policy.

INTRODUCTION: American Indians and Alaska Natives face disproportionately high rates of smoking and secondhand smoke (SHS) exposure. The Cheyenne River Sioux Tribe (CRST) is among the few Tribal Nations controlling commercial tobacco exposures in public and work places. We had an opportunity to explore effects of the new commercial tobacco-free policy (implemented in 2015) in an environmental health study (2014-2016) that collected information about commercial tobacco use and SHS prevalence and examined predictor variables of serum cotinine concentrations. METHODS: Self-reported survey data were used in quantile regression statistical modelling to explore changes in cotinine levels, based on smoking status, smokeless tobacco consumption and SHS exposure. RESULTS: From enrolled 225 adults, 51% (N=114) were current smokers. Among 88 non-tobacco users, 35 (40%) reported current SHS exposure. Significant differences in cotinine median concentrations were found among participants with and without current SHS exposure. Extremely high cotinine concentrations (~100 times larger than the median) were detected in some non-tobacco users. After implementing the new smoke-free air Tribal policy, cotinine decreased in participants with intermediate (3-15 ng/mL, non-tobacco users with SHS exposure) and high (>15 ng/mL, mainly tobacco users) cotinine levels showing association with an abatement of opportunities for SHS exposure. Significant predictors of cotinine levels were sampling year, current smoking and tobacco chewing. No gender differences were observed in cotinine. CONCLUSIONS: Our results show decrease in cotinine concentrations in CRST participants since implementation of their 'Smoke-Free Clean Air Act' in 2015.

Arsenic association with circulating oxidized low-density lipoprotein in a Native American community.

More than 500 abandoned uranium (U) mines within the Navajo Nation contribute U, arsenic (As) and other metals to groundwater, soil and potentially air through airborne transport. The adverse cardiovascular health effects attributed to cumulative exposure to these metals remains uncertain. The aim of this study was to examine whether environmental exposure to these metals may promote or exacerbate the oxidation of low-density lipoprotein (LDL) cholesterol in this Native American population. The correlation of cardiovascular biomarkers (oxidized LDL (oxLDL) and C-reactive protein (CRP)) from a Navajo cohort (n = 252) with mean annual As and U intakes from water and urine metals was estimated using linear regression. Proof-of-concept assays were performed to investigate whether As and U directly oxidize human LDL. Mean annual As intake from water was positively and significantly associated with oxLDL, but not CRP in this study population, while U intake estimates were negatively associated with oxLDL. In an acellular system, As, but not U, directly oxidized the apolipoprotein B-100 component of purified human LDL. Neither metal promoted lipid peroxidation of the LDL particle. Both the population and lab results are consistent with the hypothesis that As promotes oxidation of LDL, a crucial step in vascular inflammation and chronic vascular disease. Conversely, for outcomes related to U, negative associations were observed between U intake and oxLDL, and U only minimally altered human LDL in direct exposure experiments. Only urine U was correlated with CRP, whereas no other metals in water or urine were apparently reliable predictors of this inflammatory marker.

Residential proximity to abandoned uranium mines and serum inflammatory potential in chronically exposed Navajo communities.

Members of the Navajo Nation, who possess a high prevalence of cardiometabolic disease, reside near hundreds of local abandoned uranium mines (AUM), which contribute uranium, arsenic and other metals to the soil, water and air. We recently reported that hypertension is associated with mine waste exposures in this population. Inflammation is a major player in the development of numerous vascular ailments. Our previous work establishing that specific transcriptional responses of cultured endothelial cells treated with human serum can reveal relative circulating inflammatory potential in a manner responsive to pollutant exposures, providing a model to assess responses associated with exposure to these waste materials in this population. To investigate a potential link between exposures to AUM and serum inflammatory potential in affected communities, primary human coronary artery endothelial cells were treated for 4 h with serum provided by Navajo study participants (n=145). Endothelial transcriptional responses of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and chemokine ligand 2 (CCL2) were measured. These transcriptional responses were then linked to AUM exposure metrics, including surface area-weighted AUM proximity and estimated oral intake of metals. AUM proximity strongly predicted endothelial transcriptional responses to serum including CCL2, VCAM-1 and ICAM-1 (P<0.0001 for each), whereas annual water intakes of arsenic and uranium did not, even after controlling for all major effect modifiers. Inflammatory potential associated with proximity to AUMs, but not oral intake of specific metals, additionally suggests a role for inhalation exposure as a contributor to cardiovascular disease.

Associations of Circulating Oxidized LDL and Conventional Biomarkers of Cardiovascular Disease in a Cross-Sectional Study of the Navajo Population.

The prevalences of cardiovascular disease (CVD) and type 2 diabetes (T2D) have increased among the Navajo Native American community in recent decades. Oxidized low-density lipoprotein (oxLDL) is a novel CVD biomarker that has never been assessed in the Navajo population. We examined the relationship of oxLDL to conventional CVD and T2D risk factors and biomarkers in a cross-sectional population of Navajo participants. This cross-sectional study included 252 participants from 20 Navajo communities from the Diné Network for Environmental Health Project. Plasma samples were tested for oxLDL levels by a sandwich enzyme-linked immunosorbent assay. Univariate and multivariate analyses were used to determine the relationship of oxLDL and oxidized- to non-oxidized lipoprotein ratios to glycated hemoglobin (HbA1c), C-reactive protein (CRP), interleukin 6 (IL6) and demographic and health variables. Type 2 diabetes, hypertension and obesity are very prevalent in this Navajo population. HbA1c, CRP, body mass index (BMI), high-density lipoprotein, and triglycerides were at levels that may increase risk for CVD and T2D. Median oxLDL level was 47 (36.8-57) U/L. Correlational analysis showed that although oxLDL alone was not associated with HbA1c, oxLDL/HDL, oxLDL/LDL and CRP were significantly associated with HbA1c and glucose. OxLDL, oxLDL/HDL and oxLDL/LDL were significantly associated with CRP. Multivariate analysis showed that triglycerides were a common and strong predictor of oxLDL, oxLDL/HDL and oxLDL/LDL. OxLDL was trended with HbA1c and glucose but did not reach significance, however, HbA1c was an independent predictor of OxLDL/HDL. CRP trended with oxLDL/HDL and was a weak predictor of oxLDL/LDL. This Navajo subset appears to have oxLDL levels comparable to subjects without evidence of CVD reported in other studies. The high prevalence of T2D, hypertension and obesity along with abnormal levels of other biomarkers including HbA1c indicate that the Navajo population has a worsening CVD risk profile.

Electricity generation and nutrients removal from high-strength liquid manure by air-cathode microbial fuel cells.

Air-cathode microbial fuel cells (MFCs) are widely tested to recover electrical energy from waste streams containing organic matter. When high-strength wastewater, such as liquid animal manure, is used as a medium, inhibition on anode and cathode catalysts potentially impairs the effectiveness of MFC performance in power generation and pollutant removal. This study evaluated possible inhibitive effects of liquid swine manure components on MFC power generation, improved liquid manure-fed MFCs performance by pretreatment (dilution and selective adsorption), and modeled the kinetics of organic matter and nutrients removal kinetics. Parameters monitored included pH, conductivity, chemical oxygen demand (COD), volatile fatty acids (VFAs), total ammoniacal nitrogen (TAN), nitrite, nitrate, and phosphate concentrations. The removals of VFA and TAN were efficient, indicated by the short half-life times of 4.99 and 7.84 d, respectively. The mechanism for phosphate decrease was principally the salt precipitation on cathode, but the removal was incomplete after 42-d operation. MFC with an external resistor of 2.2 kΩ and fed with swine wastewater generated relatively small power (28.2 µW), energy efficiency (0.37%) and Coulombic efficiency (1.5%). Dilution of swine wastewater dramatically improved the power generation as the inhibitory effect was decreased. Zeolite and granular activated carbon were effective in the selective adsorption of ammonia or organic matter in swine wastewater, and so substantially improved the power generation, energy efficiency, and Coulombic efficiency. A smaller external resistor in the circuit was also observed to promote the organic matter degradation and thus to shorten the treatment time. Overall, air-cathode MFCs are promising for generating electrical power from livestock wastewater and meanwhile reducing the level of organic matter and nutrients.

Mercury, autoimmunity, and environmental factors on cheyenne river sioux tribal lands.

Mercury (Hg), shown to induce autoimmune disease in rodents, is a ubiquitous toxicant throughout Cheyenne River Sioux Tribe (CRST) lands. CRST members may be exposed to Hg through fish consumption (FC), an important component of native culture that may supplement household subsistence. Our goals were to ascertain whether total blood Hg levels (THg) reflect Hg exposure through FC and smoking, and determine whether THg is associated with the presence of anti-nuclear antibody (ANA) and specific autoantibodies (sAuAb). We recruited 75 participants who regularly consume fish from CRST waters. Hg exposure through FC and smoking were assessed via questionnaires. Whole blood samples were collected from participants, and THg was measured using ICP-MS. ANA and sAuAb in serum were modeled using demographic and exposure information as predictors. Female gender, age, and FC were significant predictors of THg and sAuAb; self-reported smoking was not. 31% of participants tested positive for ANA ≥ 2+. Although ANA was not significantly associated with Hg, the interactions of gender with Hg and proximity to arsenic deposits were statistically significant (P < 0.05). FC resulted in a detectable body burden of Hg, but THg alone did not correlate with the presence of ANA or sAuAb in this population.

Pilot-scale field study for ammonia removal from lagoon biogas using an acid wet scrubber.

The anaerobic activities in swine slurry storage and treatment generate biogas containing gaseous ammonia component which is a chemical agent that can cause adverse environmental impacts when released to the atmosphere. The aim of this pilot plant study was to remove ammonia from biogas generated in a covered lagoon, using a sulfuric acid wet scrubber. The data showed that, on average, the biogas contained 43.7 ppm of ammonia and its concentration was found to be exponentially related to the air temperature inside the lagoon. When the air temperature rose to 35°C and the biogas ammonia concentration reached 90 ppm, the mass transfer of ammonia/ammonium from the deeper liquid body to the interface between the air and liquid became a limiting factor. The biogas velocity was critical in affecting ammonia removal efficiency of the wet scrubber. A biogas flow velocity of 8 to 12 mm s(-1) was recommended to achieve a removal efficiency of greater than 60%. Stepwise regression revealed that the biogas velocity and air temperature, not the inlet ammonia concentration in biogas, affected the ammonia removal efficiency. Overall, when 73 g L(-1) (or 0.75 M) sulfuric acid solution was used as the scrubber solution, removal efficiencies varied from 0% to 100% with an average of 55% over a 40-d measurement period. Mass balance calculation based on ammonium-nitrogen concentration in final scrubber liquid showed that about 21.3 g of ammonia was collected from a total volume of 1169 m(3) of biogas, while the scrubber solution should still maintain its ammonia absorbing ability until its concentration reaches up to 1 M. These results showed promising use of sulfuric acid wet scrubber for ammonia removal in the digester biogas.

In vitro enzymatic conversion of γ-aminobutyric acid immobilization of glutamate decarboxylase with bacterial cellulose membrane (BCM) and non-linear model establishment.

The work investigated the properties and feasibility of using bacterial cellulose membrane (BCM) as a new and environmental friendly support carrier to immobilize glutamate decarboxylase (GAD) (a unique enzyme in the conversion of γ-aminobutyric acid (GABA) production). During cultivation, the porosities of BCM decreased successively with more extended fibrils piling above one another in a criss-crossing manner thus forming condensed and spatial structure. The BCM with this ultrafine network structure was found to immobilize GAD best via covalent binding because of the highest efficiency of immobilization (87.56% of the enzyme was bonded) and a good operational stability. And the covalent binding efficiency (amount of enzyme immobilized versus lost) was closely related to the porosity or the inner network of the BCM, not to the surface area. The capacity per surface area (mg/cm(2)) increased from 1.267mg/cm(2) to 3.683mg/cm(2) when the porosity of BCM ranged from 49% to 73.80%, while a declining trend of the loss of GAD specific activity (from 29.30%/cm(2) to 7.38%/cm(2)) was observed when the porosity increased from 49.9% to 72.30%. Two non-linear regression relationships, between the porosity and loading capacity and between porosity and enzyme activity loss, were empirically modeled with the determination of coefficient R(2) of 0.980 and 0.977, respectively. Finally, the established in vitro enzymatic conversion process demonstrated 6.03g/L of GABA at 0.10mol/L Glu, 60min of retention time and 160mL of suspension volume after the 1st run and a loss of 4.15% after the 4th run. The productivity of GABA was 6.03gL(-1)h(-1), higher than that from other reported processes.

Kinetics study of fermentative hydrogen production from liquid swine manure supplemented with glucose under controlled pH.

Kinetics of H2 production from liquid swine manure supplemented with glucose by mixed anaerobic cultures was investigated using batch experiments under four different pH conditions (4.4, 5.0, 5.6, and uncontrolled). The temperature for the experiments was controlled at 37 ± 1°C and the length of experiments varied between 50 and 120 hours, depending upon the time needed for completion of each individual experiment. The modified Gompertz model was evaluated for its suitability for describing the H2 production potential, H2 production rate, and substrate consumption rate for all the experiments. The results showed that the Gompertz model could adequately fit the experimental results. The effect of pH was significant on all kinetic parameters for H2 production including yield, production rate and lag time, and the substrate utilization rate. The optimal pH was found to be 5.0, at which a maximum H2 production rate (0.64 L H2/h) was obtained, and deviation from the optimal pH could result in substantial reductions in H2 production rate (0.32 L H2/h for pH 4.0 and 0.43 L H2/h for pH 5.6). The results also showed that if pH was not controlled for the batch fermentation process, the substrate utilization efficiency could steeply decrease from 98.8% to 33.7%.

Aeration efficiency influenced by venturi aerator arrangement, liquid flow rate and depth of diffusing pipes.

The effect of aerator module configuration, liquid flow rate and diffuser submergence on oxygen transfer efficiency was examined in a surface aeration system with venturi injectors using the clean water test. Six venturi aerator modules were evaluated and the results indicated that better aeration efficiencies could be achieved by simply changing the way the venturi aerators were connected. Among all the configurations examined (modules a-f), two and three aerators connected in parallel (modules d, e and f) were able to bring more oxygen into water than the others. An increase in liquid flow rate led to an enhancement of the oxygen transfer coefficients, but the improvement was reduced if the liquid flow rate was too high. The oxygen transfer coefficient was found to have a relationship with the depth of diffusing pipes (surface aeration depth) for the surface aeration system and an optimal depth of around 40 cm was obtained from this study.

System establishment of ATPS for one-step purification of glutamate decarboxylase from E. coli after cell disruption.

The partition of glutamate decarboxylase (GAD) from Escherichia coli in polyethylene glycol (PEG) and sodium sulfate aqueous two-phase systems (ATPS) has been explored with the purpose of establishing a phase system for the purification of GAD after cell disruption. The results showed that the partitioning of GAD was slightly influenced by PEG molecular weight (MW) but depended on the tie line length (TLL) and NaCl and loading sample concentrations. The optimum system obtained for GAD purification was composed of a PEG MW of 4,000, TLL of 63.5%, a volume ratio of 2.31, a loading sample concentration of 0.4 g/mL, which produced a GAD recovery of 90% with the purification fold of 73. Furthermore, the feasibility of directly purifying GAD from the cell disrupts using ATPS was evaluated. The established ATPS for GAD purification exhibited an efficient integrated purification process compared to the reported purification process in terms of purification efficiency and recovery.

The stability of accumulating nitrite from Swine wastewater in a sequencing batch reactor.

Shortcut nitrification is the first step of shortcut nitrogen removal from swine wastewater. Stably obtaining an effluent with a significant amount of nitrite is the premise for the subsequent shortcut denitrification. In this paper, the stability of nitrite accumulation was investigated using a 1.5-day hydraulic retention time in a 10-L (working volume) activated sludge sequencing batch reactor (SBR) with an 8-h cycle consisted of 4 h 38 min aerobic feeding, 1 h 22 min aerobic reaction, 30 min settling, 24 min withdrawal, and 1 h 6 min idle. The nitrite production stability was tested using four different ammonium loading rates, 0.075, 0.062, 0.053, and 0.039 g NH(4)-N/g (mixed liquid suspended solid, MLSS) day in a 2-month running period. The total inorganic nitrogen composition in the effluent was not affected when the ammonium load was between 0.053 and 0.075 g NH(4)-N/g MLSS · day (64% NO(2)-N, 16% NO(3)-N, and 20% NH(4)-N). Under 0.039 g NH(4)-N/g MLSS · day, more NO(2)-N was transformed to NO(3)-N with an effluent of 60% NO(2)-N, 20% NO(3)-N, and 20% NH(4)-N. The reducing load test was able to show the relationship between a declining free nitrous acid (FNA) concentration and the decreasing nitrite production, indicating that the inhibition of FNA on nitrite oxidizing bacteria depends on its levels and an ammonium loading rate around 0.035 g NH(4)-N/g MLSS · day is the lower threshold for producing a nitrite dominance effluent in the activated sludge SBR under the current settings.

Biological and chemical phosphorus fractionalization in swine manure under aeration.

In this work, the dynamic responses of different phosphorous factions in swine manure to aeration treatment were investigated and profiled to provide insight on potential ways to improve the biological phosphorus removal process. Batch reactors fabricated from clear acrylic columns were filled with fresh swine manure containing a 4.6 % solids content, which was aerated continuously for 15 days at an airflow rate of 2 L/min. The results indicate that the treatment can reduce soluble phosphorus (P) by about 78 % after only one-day aeration due largely to chemical precipitations. At the end of the experiment, the average soluble inorganic P level was reduced by 12 % (from 91 down to 79 %), while the average soluble organic P was increased by 13 % (from 9 to 22 %). The biomass P (DNA/RNA/poly-P) was increased by 24 % in the first three days, but only by 14 % in the rest 12 days of aeration. Also increased by aeration was the lipid P (47 %) but not the protein P. The data reveal that the current aeration rate cannot maintain a stable oxygen level [represented by the oxidation-reduction potential (ORP)] in the treated manure, evidenced by the decrease in ORP from 250 mV at the beginning to almost zero at the conclusion of the experiment, which is considered the major factor hindering the growth of aerobes, including the phosphorus-accumulating organisms (PAOs). Therefore, it may be concluded that continuous aeration of swine manure at a constant rate will not guarantee the supply of sufficient oxygen to the growth of PAOs. On another front, it was observed that too much aeration might negatively impact the overall P removal by increasing the release of soluble organic P from dead cells.

Biogas and CH(4) productivity by co-digesting swine manure with three crop residues as an external carbon source.

Co-digesting swine manure with three agricultural residues, i.e., corn stalks, oat straw, and wheat straw, to enhance biogas productivity was investigated in this study. A 3x3 experimental design with duplicates was adopted (3 crop residuesx3 carbon/nitrogen ratios) to examine the improvement of batch digestion in terms of biogas volume produced, CH(4) content in the biogas, and net CH(4) volume. The crop residues were first cut into small sections and then ground into fine particles smaller than 40 mesh size (0.422mm) before being added to digesters. All the digesters were run simultaneously under controlled temperature at 37+/-0.1 degrees C. The length of experiment was 25days. The results showed that all crop residues significantly increased biogas production and net CH(4) volume at all C/N ratios, among which corn stalks performed the best with increase in daily maximum biogas volume by 11.4-fold as compared to the control, followed by oat straw (8.45-fold) and wheat straw (6.12-fold) at the C/N ratio of 20/1, which was found to be the optimal C/N ratio for co-digestion in the present study. In addition, corn stalks achieved the highest CH(4) content in the biogas ( approximately 68%), which was about 11% higher than that of oat straw ( approximately 57%), whereas wheat straw and the control both had produced biogas with approximately 47% CH(4) content. Wheat straw demonstrated a lower biogas productivity than corn stalks and oat straw even it had a higher carbon content (46%) than the latter two residues (39%).

Utilization of protein extract from dairy manure as a nitrogen source by Rhizopus oryzae NRRL-395 for l-lactic acid production.

Six levels of crude protein (0.21, 0.42, 0.84, 1.68, 2.52, and 3.36g/L) and six levels of protein hydrolysates from dairy manure, defined by degree of hydrolysis (DH, 6.9%, 17.2%, 25.9%, 33.8%, 36.1%, and 36.7%), were investigated as the nitrogen source for production of l-lactic acid by Rhizopus oryzae NRRL-395 with respect to the influence of nitrogen source on l-lactic acid yield and the correlation with biomass yield and mycelia morphology. Increases in crude protein from 0.21 to 1.68g/L led to an increase in l-lactic acid concentration in the culture media from 6.48 to 57.7g/L. However, further increases beyond 1.68g/L did not present continuing increases in l-lactic acid yields. The highest biomass yield was obtained at a crude protein nitrogen concentration of 2.52g/L. Hydrolysates with high DH resulted in high yields of l-lactic acid and biomass. At a nitrogen level of 0.42g/L (hydrolysates) with DH ranging from 33.8% to 36.7%, the l-lactic acid yield of 0.53-0.56g/g of glucose was achieved, coupled with a 13-14% yield of fungal biomass.