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Introduction: Sanjin tablets (SJT) are a well-known Chinese patent drug that have been used to treat urinary tract infections (UTIs) for the last 40 years. The drug consists of five herbs, but only 32 compounds have been identified, which hinders the clarification of its effective substances and mechanism. Methods: The chemical constituents of SJT and their effective substances and functional mechanism involved in the treatment of UTIs were investigated by using high performance liquid chromatography-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-ESI-IT-TOF-MSn), network pharmacology, and molecular docking. Results: A total of 196 compounds of SJT (SJT-MS) were identified, and 44 of them were unequivocally identified by comparison with the reference compounds. Among 196 compounds, 13 were potential new compounds and 183 were known compounds. Among the 183 known compounds, 169 were newly discovered constituents of SJT, and 93 compounds were not reported in the five constituent herbs. Through the network pharmacology method, 119 targets related to UTIs of 183 known compounds were predicted, and 20 core targets were screened out. Based on the "compound-target" relationship analysis, 94 compounds were found to act on the 20 core targets and were therefore regarded as potential effective compounds. According to the literature, 27 of the 183 known compounds were found to possess antimicrobial and anti-inflammatory activities and were verified as effective substances, of which 20 were first discovered in SJT. Twelve of the 27 effective substances overlapped with the 94 potential effective compounds and were determined as key effective substances of SJT. The molecular docking results showed that the 12 key effective substances and 10 selected targets of the core targets have good affinity for each other. Discussion: These results provide a solid foundation for understanding the effective substances and mechanism of SJT.
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Objective To evaluate the immune effect of hepatitis B vaccine by different immunization schedules among adults,and to provide evidence for developing immunization strategy.Methods Adults aged 1 6 to 49 years with negative HBsAg and anti -HBs in Tongxiang city voluntarily received domestic or imported hepatitis B vaccine.These subjects were divided into 4 groups (Group A:1 0 μg domestic vaccine,0 -1 -3 schedule;group B:1 0 μg domestic vaccine,0 -1 -6 schedule;group C:1 0 μg imported vaccine,0 -1 -3 schedule;group D:1 0 μg imported vaccine,0 -1 -6 schedule). Quantitative detection of anti -HBs was conducted in 1 month and 1 year,and the anti -HBs positive rate and geometric mean titers of anti -HBs (GMT)were evaluated.Results A total of 848 subjects were evaluated.The anti -HBs positive rate was 99.76% after immunization in 1 month,and no significant differences were observed among different groups (P >0.05).The GMT of anti -HBs in group A was significantly lower than that of group C and B (P <0.05),while no significant differences were observed among group B and D.The GMT of anti -HBs was significantly decreased with ages. The anti -HBs positive rate was 70.28% after immunization in 1 year,which was significantly lower than that in 1 month after immunization.Conclusion Better immune effect could be achieved by 1 0 μg domestic hepatitis B vaccine with 0 -1-3 schedule.
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Objective] To evaluate the immune effect of hepatitis B vaccine by different inocula-ting programs for adults as 0-1-3 month, 0-1-6 month and 0-1-12 month and to provide evidence for the development of adult immunization strategy. [ Methods] In Tongxiang City of Zhejiang Province, 10μg of hepatitis B vaccine was voluntarily inoculated by different immunization programs as 0 -1 -3 month, 0-1-6 month or 0 -1 -12 month among adults aged 16 to 49 years.Quantitative detection of anti-HBs was conducted after 1 month and 1 year of immunization, and the positive seroconversion rates and geometric mean titers of anti-HBs ( GMT) were evaluated. [ Results] Totally 682 subjects were evaluated.The anti-HBs positive rates were 99.85% after 1 month of immunization, and 70.23% after 1 year of immunization.It was observed that GMT of anti-HBs was higher with adults of younger age. [ Conclusion] Adults can achieve better immune effect by inoculation of 10μg of hepatitis B vaccine and when it is done in target population according to 0-1-6 month program,compliance is better and immune effect proves more persistent.It is suggested that hepatitis B vaccine should be inoculated for the adults as early as possible to ensure the effect and persistence of the vaccine.
