Experimental duration determines the effect of arbuscular mycorrhizal fungi on plant biomass in pot experiments: A meta-analysis.

Arbuscular mycorrhizal fungi (AMF) play various important roles in promoting plant growth. Numerous environmental and evolutionary factors influence the response of plants to AMF. However, the importance of the individual factors on the effects of AMF on plant biomass is not clearly understood. In this study, a meta-analysis using 1,640 observations from 639 published articles related to the influence of AMF on the plant shoot, root, and total biomass was performed; 13 different experimental setting factors that had an impact on the influence of AMF and their importance were quantitatively synthesized. The meta-analysis showed that AMF had positive effects on the plant shoot, root, and total biomass; moreover, the experimental duration, plant root-to-shoot ratio (R/S), AMF root length colonization, plant family, pot size, soil texture, and the soil pH all influenced the effects of AMF on the shoot, root, and total biomass. In addition, the plant root system and plant functional type had impacts on the effect of AMF on shoot biomass; AMF guild also impacted the effect of AMF on root biomass. Of these factors, the experimental duration, plant R/S, and pot size were the three most important predicting the effects of AMF on the plant shoot, root, and total biomass. This study comprehensively assessed the importance of the different factors that influenced the response of plants to AMF, highlighting that the experimental duration, plant R/S, and pot size should be taken into consideration in pot experiments in studies of the functions of AMF. Multiple unfavorable factors that may obscure or confound the observed functions of AMF should be excluded.

Pathogenic Microbes Increase Plant Dependence on Arbuscular Mycorrhizal Fungi: A Meta-Analysis.

Numerous studies have confirmed that arbuscular mycorrhizal fungi (AMF) can promote plant nitrogen and phosphorus absorption, and prime systemic plant defense to plant pathogenic microbes. Despite that, the information on the interaction between AMF and plant pathogenic microbes is limited, especially the influence of plant pathogenic microbes on the effect of AMF promoting plant growth. In this study, 650 independent paired-wise observations from 136 published papers were collected and used to calculate the different effect of AMF with plant pathogenic microbes (DAPP) in promoting plant growth through meta-analysis. The results showed that AMF had a higher effect size on plant growth with pathogenic microbes comparing to without pathogenic microbes, including the significant effects in shoot and total fresh biomass, and shoot, root, and total dry biomass. The results of the selection models revealed that the most important factor determining the DAPP on plant dry biomass was the harm level of plant pathogenic microbes on the plant dry biomass, which was negatively correlated. Furthermore, the change of AMF root length colonization (RLC) was the sub-important factor, which was positively correlated with the DAPP. Taken together, these results have implications for understanding the potential and application of AMF in agroecosystems.

Revegetation differentially influences microbial trophic groups in a Qinghai-Tibetan alpine steppe ecosystem.

Revegetation accelerates the recovery of degraded lands. Different microbial trophic groups underpin this acceleration from the aspects of soil structure stabilization, nutrient accumulation, and ecosystem functions. However, little is known about how revegetation influences the community and biodiversity of different soil microbial trophic groups. Here, six revegetation treatments with different plantings of plant species were established at an excavation pit in the Qinghai-Tibetan Plateau. Communities of plant, bacteria, and several key soil fungal groups were investigated after 12 years of revegetation. Plant and all microbial trophic group compositions were markedly influenced by revegetation treatments. Total fungal and pathogenic fungal compositions were not significantly predicted by any factor of plant and soil, but arbuscular mycorrhizal fungal composition could be mainly predicted by plant composition and plant P content. Bacterial composition was mainly determined by soil total N, organic carbon concentration, and moisture content; and saprotrophic fungal composition was mainly determined by soil organic carbon. Soil pH was the strongest factor to predict bacterial metabolic functions. Our findings highlight that even the differences of microbial compositions were because of different revegetation treatments, but each trophic microbial composition had different relations with plant and/or soil; especially, the bacterial community and metabolic functions and saprotrophic fungal community were more correlated with soil properties rather than plant community or characteristics per se.

Aphids Influence Soil Fungal Communities in Conventional Agricultural Systems.

Arbuscular mycorrhizal fungi (AMF) form symbioses with the roots of most plant species, including cereals. AMF can increase the uptake of nutrients including nitrogen (N) and phosphorus (P), and of silicon (Si) as well as increase host resistance to various stresses. Plants can simultaneously interact with above-ground insect herbivores such as aphids, which can alter the proportion of plant roots colonized by AMF. However, it is unknown if aphids impact the structure of AMF communities colonizing plants or the extent of the extraradical mycelium produced in the soil, both of which can influence the defensive and nutritional benefit a plant derives from the symbiosis. This study investigated the effect of aphids on the plant-AMF interaction in a conventionally managed agricultural system. As plants also interact with other soil fungi, the non-AMF fungal community was also investigated. We hypothesized that aphids would depress plant growth, and reduce intraradical AMF colonization, soil fungal hyphal density and the diversity of AM and non-AM fungal communities. To test the effects of aphids, field plots of barley enclosed with insect proof cages were inoculated with Sitobion avenae or remained uninoculated. AMF specific and total fungal amplicon sequencing assessed root fungal communities 46 days after aphid addition. Aphids did not impact above-ground plant biomass, but did increase the grain N:P ratio. Whilst aphid presence had no impact on AMF intraradical colonization, soil fungal hyphal length density, or AMF community characteristics, there was a trend for the aphid treatment to increase vesicle numbers and the relative abundance of the AMF family Gigasporaceae. Contrary to expectations, the aphid treatment also increased the evenness of the total fungal community. This suggests that aphids can influence soil communities in conventional arable systems, a result that could have implications for multitrophic feedback loops between crop pests and soil organisms across the above-below-ground interface.