RESUMEN
Antibodies against foot-and-mouth disease virus serotypes A, O and Asia-1 were detected by ELISA in liquid and strip-dried samples from vaccinated bovines. The results showed high concordance of the results in both types of serum samples (coefficient of correlation varied from 0.89 to 0.96). Stability studies showed that anti-foot-and-mouth disease virus antibodies can be detected in strip-dried serum samples stored at different temperatures on a level of that in native samples. Preliminary study in strip-dried whole blood samples demonstrated good potential of this sample pretreatment procedure for the following anti-foot-and-mouth virus antibodies testing.
Asunto(s)
Anticuerpos Antivirales/sangre , Pruebas con Sangre Seca/veterinaria , Virus de la Fiebre Aftosa/clasificación , Virus de la Fiebre Aftosa/inmunología , Animales , Biotecnología , Bovinos , Enfermedades de los Bovinos/inmunología , Enfermedades de los Bovinos/prevención & control , Pruebas con Sangre Seca/métodos , Ensayo de Inmunoadsorción Enzimática , Fiebre Aftosa/inmunología , Fiebre Aftosa/prevención & control , Serogrupo , Vacunación/veterinariaRESUMEN
Phylogenetic analyses of foot-and-mouth disease type A viruses in the Middle East during 2015-2016 identified viruses belonging to the A/ASIA/G-VII lineage, which originated in the Indian subcontinent. Changes in a critical antigenic site within capsid viral protein 1 suggest possible evolutionary pressure caused by an intensive vaccination program.