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Introduction Columnar lined epithelium (CLE) of the esophagus holds particular importance in diagnosing Barrett's esophagus (BE). In Asia, the prevalence of BE ranges from 0.06% to 6.2%. In India, prevalence estimates vary from 2.6% to 23%. The frequency of esophageal adenocarcinoma has also been observed to be increasing alarmingly over the past few decades. The length of CLE as a criterion for diagnosing BE is the subject of considerable debate. Changes in CLE length among different age groups may exist. Our study aims to measure the length of CLE, or the distance between the angle of His and the Z Line (AZ distance), in normal individuals from Northeast India, and to analyze its variation across different age groups. Materials and methods The study was conducted in the Department of Anatomy and the Department of Forensic Medicine and Toxicology at Gauhati Medical College, Guwahati, Assam, India, during the period 2017-2019. Once opened, each specimen was laid flat on a board. The distance between the A and Z lines was measured using a pair of vernier calipers. This distance was recorded as the AZ distance in millimeters (mm). Results The mean AZ Distance was found to be 12.4 ± 5.3 mm. A significant correlation between age and AZ distance was observed. Conclusion Our present study suggests that the length of the CLE increases with age. This observation offers an opportunity to revisit or revise the diagnostic criteria based on CLE length, taking into account the age of the individual.
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PURPOSE: The ER chaperone GRP78 translocates to the surface of tumor cells and promotes survival, metastasis, and resistance to therapy. An oncogenic function of cell surface GRP78 has been attributed to the activation of the phosphoinositide 3-kinase (PI3K) pathway. We intend to use a novel anti-GRP78 monoclonal antibody (MAb159) to attenuate PI3K signaling and inhibit tumor growth and metastasis. EXPERIMENTAL DESIGN: MAb159 was characterized biochemically. Antitumor activity was tested in cancer cell culture, tumor xenograft models, tumor metastasis models, and spontaneous tumor models. Cancer cells and tumor tissues were analyzed for PI3K activity. MAb159 was humanized and validated for diagnostic and therapeutic application. RESULTS: MAb159 specifically recognized surface GRP78, triggered GRP78 endocytosis, and localized to tumors but not to normal organs in vivo. MAb159 inhibited tumor cell proliferation and enhanced tumor cell death both in vitro and in vivo. In MAb159-treated tumors, PI3K signaling was inhibited without compensatory MAPK pathway activation. Furthermore, MAb159 halted or reversed tumor progression in the spontaneous PTEN-loss-driven prostate and leukemia tumor models, and inhibited tumor growth and metastasis in xenograft models. Humanized MAb159, which retains high affinity, tumor specific localization, and the antitumor activity, was nontoxic in mice, and had desirable pharmacokinetics. CONCLUSIONS: GRP78-specific antibody MAb159 modulates the PI3K pathway and inhibits tumor growth and metastasis. Humanized MAb159 will enter human trials shortly.
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Anticuerpos Monoclonales Humanizados/administración & dosificación , Anticuerpos Monoclonales/administración & dosificación , Proteínas de Choque Térmico/genética , Neoplasias/tratamiento farmacológico , Transducción de Señal/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Elafina/genética , Chaperón BiP del Retículo Endoplásmico , Células HT29 , Proteínas de Choque Térmico/inmunología , Humanos , Ratones , Metástasis de la Neoplasia , Neoplasias/inmunología , Neoplasias/patología , Proteínas Proto-Oncogénicas c-akt/genética , Transducción de Señal/inmunología , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
The Wnt/ß-catenin pathway, which signals through the Frizzled (Fzd) receptor family and several coreceptors, has long been implicated in cancer. Here we demonstrate a therapeutic approach to targeting the Wnt pathway with a monoclonal antibody, OMP-18R5. This antibody, initially identified by binding to Frizzled 7, interacts with five Fzd receptors through a conserved epitope within the extracellular domain and blocks canonical Wnt signaling induced by multiple Wnt family members. In xenograft studies with minimally passaged human tumors, this antibody inhibits the growth of a range of tumor types, reduces tumor-initiating cell frequency, and exhibits synergistic activity with standard-of-care chemotherapeutic agents.
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Anticuerpos Monoclonales/farmacología , Antineoplásicos/farmacología , Receptores Frizzled/metabolismo , Neoplasias/tratamiento farmacológico , Vía de Señalización Wnt/efectos de los fármacos , Animales , Anticuerpos Monoclonales/metabolismo , Antineoplásicos/metabolismo , Western Blotting , Células CHO , Cricetinae , Cricetulus , Sinergismo Farmacológico , Vectores Genéticos/genética , Células HEK293 , Humanos , Fragmentos Fab de Inmunoglobulinas/genética , Inmunohistoquímica , Lentivirus , Luciferasas , Neoplasias/metabolismo , Biblioteca de Péptidos , Vía de Señalización Wnt/fisiologíaRESUMEN
Focal adhesion kinase (FAK) is a cytoplasmic protein-tyrosine kinase that promotes cell migration, survival, and gene expression. Here we show that FAK signaling is important for tumor necrosis factor-alpha (TNFalpha)-induced interleukin 6 (IL-6) mRNA and protein expression in breast (4T1), lung (A549), prostate (PC-3), and neural (NB-8) tumor cells by FAK short hairpin RNA knockdown and by comparisons of FAK-null (FAK(-/-)) and FAK(+/+) mouse embryo fibroblasts. FAK promoted TNFalpha-stimulated MAPK activation needed for maximal IL-6 production. FAK was not required for TNFalpha-mediated nuclear factor-kappaB or c-Jun N-terminal kinase activation. TNFalpha-stimulated FAK catalytic activation and IL-6 production were inhibited by FAK N-terminal but not FAK C-terminal domain overexpression. Analysis of FAK(-/-) fibroblasts stably reconstituted with wild type or various FAK point mutants showed that FAK catalytic activity, Tyr-397 phosphorylation, and the Pro-712/713 proline-rich region of FAK were required for TNFalpha-stimulated MAPK activation and IL-6 production. Constitutively activated MAPK kinase-1 (MEK1) expression in FAK(-/-) and A549 FAK short hairpin RNA-expressing cells rescued TNFalpha-stimulated IL-6 production. Inhibition of Src protein-tyrosine kinase activity or mutation of Src phosphorylation sites on FAK (Tyr-861 or Tyr-925) did not affect TNFalpha-stimulated IL-6 expression. Moreover, analyses of Src(-/-), Yes(-/-), and Fyn(-/-) fibroblasts showed that Src expression was inhibitory to TNFalpha-stimulated IL-6 production. These studies provide evidence for a novel Src-independent FAK to MAPK signaling pathway regulating IL-6 expression with potential importance to inflammation and tumor progression.
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Proteína-Tirosina Quinasas de Adhesión Focal/metabolismo , Interleucina-6/metabolismo , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Transducción de Señal/fisiología , Factor de Necrosis Tumoral alfa/metabolismo , Animales , Línea Celular Tumoral , Activación Enzimática , Fibroblastos/citología , Fibroblastos/metabolismo , Proteína-Tirosina Quinasas de Adhesión Focal/genética , Interleucina-6/genética , Ratones , Ratones Noqueados , Proteínas Quinasas Activadas por Mitógenos/genética , FN-kappa B/metabolismo , Prolina/metabolismo , Interferencia de ARN , Tirosina/metabolismo , Familia-src Quinasas/metabolismoRESUMEN
Integrins can alter cellular behavior through the recruitment and activation of signaling proteins such as non-receptor tyrosine kinases including focal adhesion kinase (FAK) and c-Src that form a dual kinase complex. The FAK-Src complex binds to and can phosphorylate various adaptor proteins such as p130Cas and paxillin. In normal cells, multiple integrin-regulated linkages exist to activate FAK or Src. Activated FAK-Src functions to promote cell motility, cell cycle progression and cell survival. Recent studies have found that the FAK-Src complex is activated in many tumor cells and generates signals leading to tumor growth and metastasis. As both FAK and Src catalytic activities are important in promoting VEGF-associated tumor angiogenesis and protease-associated tumor metastasis, support is growing that FAK and Src may be therapeutically relevant targets in the inhibition of tumor progression.
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Proteína-Tirosina Quinasas de Adhesión Focal/metabolismo , Integrinas/metabolismo , Neoplasias/metabolismo , Transducción de Señal/fisiología , Familia-src Quinasas/metabolismo , Animales , Movimiento Celular/fisiología , Supervivencia Celular , Progresión de la Enfermedad , Activación Enzimática , Neoplasias/patologíaRESUMEN
The fibronectin binding integrins alpha5beta1 and alpha4beta1 generate signals pivotal for cell migration through distinct yet undefined mechanisms. For alpha5beta1, beta1-mediated activation of focal adhesion kinase (FAK) promotes c-Src recruitment to FAK and the formation of a FAK-Src signaling complex. Herein, we show that FAK expression is essential for alpha5beta1-stimulated cell motility and that exogenous expression of human alpha4 in FAK-null fibroblasts forms a functional alpha4beta1 receptor that promotes robust cell motility equal to the alpha5beta1 stimulation of wild-type and FAK-reconstituted fibroblasts. alpha4beta1-stimulated FAK-null cell spreading and motility were dependent on the integrity of the alpha4 cytoplasmic domain, independent of direct paxillin binding to alpha4, and were not affected by PRNK expression, a dominant-negative inhibitor of Pyk2. alpha4 cytoplasmic domain-initiated signaling led to a approximately 4-fold activation of c-Src which did not require paxillin binding to alpha4. Notably, alpha4-stimulated cell motility was inhibited by catalytically inactive receptor protein-tyrosine phosphatase alpha overexpression and blocked by the p50Csk phosphorylation of c-Src at Tyr-529. alpha4beta1-stimulated cell motility of triple-null Src(-/-), c-Yes(-/-), and Fyn(-/-) fibroblasts was dependent on c-Src reexpression that resulted in p130Cas tyrosine phosphorylation and Rac GTPase loading. As p130Cas phosphorylation and Rac activation are common downstream targets for alpha5beta1-stimulated FAK activation, our results support the existence of a novel alpha4 cytoplasmic domain connection leading to c-Src activation which functions as a FAK-independent linkage to a common motility-promoting signaling pathway.
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Movimiento Celular/fisiología , Fibroblastos/fisiología , Proteína-Tirosina Quinasas de Adhesión Focal/metabolismo , Integrina alfa4beta1/fisiología , Proteínas Tirosina Quinasas/fisiología , Secuencia de Aminoácidos , Animales , Proteína Tirosina Quinasa CSK , Línea Celular , Fibroblastos/metabolismo , Proteína-Tirosina Quinasas de Adhesión Focal/genética , Humanos , Integrina alfa4beta1/genética , Integrina alfa5beta1/metabolismo , Ratones , Datos de Secuencia Molecular , Fosforilación , Proteínas Proto-Oncogénicas c-fyn/genética , Proteínas Proto-Oncogénicas c-fyn/metabolismo , Proteínas Proto-Oncogénicas c-yes/genética , Proteínas Proto-Oncogénicas c-yes/metabolismo , Transducción de Señal/fisiología , Familia-src QuinasasRESUMEN
A central question in cell biology is how membrane-spanning receptors transmit extracellular signals inside cells to modulate cell adhesion and motility. Focal adhesion kinase (FAK) is a crucial signalling component that is activated by numerous stimuli and functions as a biosensor or integrator to control cell motility. Through multifaceted and diverse molecular connections, FAK can influence the cytoskeleton, structures of cell adhesion sites and membrane protrusions to regulate cell movement.
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Movimiento Celular/fisiología , Proteínas Tirosina Quinasas/fisiología , Animales , Membrana Celular/fisiología , Proteínas del Citoesqueleto/fisiología , Citoesqueleto/fisiología , Quinasa 1 de Adhesión Focal , Proteína-Tirosina Quinasas de Adhesión Focal , Humanos , Uniones Intercelulares/fisiología , Paxillin , Fosfoproteínas/fisiología , Fosforilación , Estructura Terciaria de Proteína , Proteínas/fisiología , Proteína p130 Similar a la del RetinoblastomaRESUMEN
Cell motility is stimulated by extracellular stimuli and initiated by intracellular signaling proteins that localize to sites of cell contact with the extracellular matrix termed focal contacts. Focal adhesion kinase (FAK) is an intracellular protein-tyrosine kinase (PTK) that acts to regulate the cycle of focal contact formation and disassembly required for efficient cell movement. FAK is activated by a variety of cell surface receptors and transmits signals to a range of targets. Thus, FAK acts as an integrator of cell motility-associated signaling events. We will review the stimulatory and regulatory mechanisms of FAK activation, the different signaling connections of FAK that are mediated by a growing number of FAK-interacting proteins, and the modulation of FAK function by tyrosine and serine phosphorylation. We will also summarize findings with regard to FAK function in vertebrate and invertebrate development as well as recent insights into the mechanistic role(s) of FAK in promoting cell migration. As increased FAK expression and tyrosine phosphorylation have been correlated with the progression to an invasive cell phenotype, there is growing interest in elucidating the important FAK-related signaling connections promoting invasive tumor cell movement. To this end, we will discuss the effects of FAK inhibition via the dominant-negative expression of the FAK C-terminal domain termed FAK-related non-kinase (FRNK) and how these studies have uncovered a distinct role for FAK in promoting cell invasion that may differ from its role in promoting cell motility.
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Movimiento Celular/fisiología , Invasividad Neoplásica/genética , Proteínas Tirosina Quinasas/fisiología , Secuencia de Aminoácidos , Animales , Sitios de Unión , Membrana Celular/química , Membrana Celular/metabolismo , Activación Enzimática , Fibroblastos/química , Fibroblastos/metabolismo , Fibroblastos/patología , Quinasa 1 de Adhesión Focal , Proteína-Tirosina Quinasas de Adhesión Focal , Adhesiones Focales/química , Adhesiones Focales/metabolismo , Humanos , Datos de Secuencia Molecular , Mutación , Metástasis de la Neoplasia , Fenotipo , Proteínas Tirosina Quinasas/química , Proteínas Tirosina Quinasas/genética , Proteínas Tirosina Quinasas/metabolismo , Transducción de SeñalRESUMEN
The ability of intracellular signaling networks to orchestrate a complex biological response such as cell motility requires that individual signaling proteins must act as integrators, responding to multiple extracellular inputs and regulating multiple signaling pathway outputs. In this review, we highlight recent findings that place focal adhesion kinase (FAK) in an important receptor-proximal position in the regulation of growth factor and integrin-stimulated cell motility. Emphasis is placed on the molecular mechanisms of FAK activation, connections of FAK to focal contact formation as well as turnover, and the potential that FAK function in promoting cell invasion may be distinct from its role in cell motility.
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Movimiento Celular/fisiología , Proteínas Tirosina Quinasas/fisiología , Transducción de Señal , Activación Enzimática , Fibroblastos/fisiología , Proteína-Tirosina Quinasas de Adhesión Focal , Integrinas/fisiología , FosforilaciónRESUMEN
Cell migration and invasion are fundamental components of tumor cell metastasis. Increased focal adhesion kinase (FAK) expression and tyrosine phosphorylation are connected with elevated tumorigenesis. Null mutation of FAK results in embryonic lethality, and FAK-/- fibroblasts exhibit cell migration defects in culture. Here we show that viral Src (v-Src) transformation of FAK-/- cells promotes integrin-stimulated motility equal to stable FAK reexpression. However, FAK-/- v-Src cells were not invasive, and FAK reexpression, Tyr-397 phosphorylation, and FAK kinase activity were required for the generation of an invasive cell phenotype. Cell invasion was linked to transient FAK accumulation at lamellipodia, formation of a FAK-Src-p130Cas-Dock180 signaling complex, elevated Rac and c-Jun NH2-terminal kinase activation, and increased matrix metalloproteinase expression and activity. Our studies support a dual role for FAK in promoting cell motility and invasion through the activation of distinct signaling pathways.