Biofilm and suspension-based cultivation of microalgae to treat anaerobic digestate food effluent (ADFE).

Anaerobic digestion of organic waste produces effluent (ADE) that requires further treatment. Biofilm-based microalgal cultivation is a favoured approach to ADE treatment. This study compared Chlorella sp. MUR 268 and Scenedesmus sp. MUR 269 in biofilm and suspension cultures to treat anaerobic digestate food effluent (ADFE). Chlorella sp. MUR 268 biofilm had significantly higher biomass (50.38 g m-2) than Scenedesmus sp. biofilm (9.39 g m-2). Conversely, Scenedesmus sp. yielded 1.5 times more biomass (1.2 g L-1) than Chlorella sp. in suspension. Chlorella sp. biofilm had 49.3 % higher areal productivity than suspension, while Scenedesmus sp. showed 87.3 % higher areal growth in suspension. Chlorella sp. MUR 268 and Scenedesmus sp. MUR 269 significantly removed nutrients in ADFE. In suspension, COD, ammoniacal nitrogen, and phosphate were reduced to 94.9, 5.2, and 5.98 mg L-1 for Chlorella sp. MUR 268, and 245, 2.89, and 3.22 mg L-1 for Scenedesmus sp. MUR 269, respectively. In biofilm, Chlorella sp. MUR 268 achieved reductions to 149.9, 1.16, and 3.57 mg L-1, while Scenedesmus sp. MUR 269 achieved 100.2, 6.9 and 2.07 mg L-1. Most of these values are below the recommended effluent discharge standard, highlighting the efficacy of this system in ADFE treatment. Biofilm cultures fixed 68-81 % of removed nitrogen in biomass, while in suspension, only 55-71 % ended in the biomass. Chlorella sp. MUR 268 biofilm fixed 88 % of removed phosphorus, while Scenedesmus sp. MUR 269 suspension fixed more phosphorus (55 %) than the biofilm counterpart (34 %). This biofilm design offers advantages like simplified, cost-effective operation, easy biomass recovery, and reduced water usage.

Biofilm cultivation of chlorella species. MUR 269 to treat anaerobic digestate food effluent (ADFE): Total ammonia nitrogen (TAN) concentrations effect.

Microalgal-based treatment of anaerobic digestate food effluent (ADFE) has been found to be efficient and effective. However, turbidity and high total ammonia nitrogen (TAN)) content of ADFE is a major setback, requiring significant dilution. Although the possibility of growing microalgae in a high-strength ADFE with minimal dilution has been demonstrated in suspension cultures, such effluents remain highly turbid and affect the light path in suspension cultures. Here, the feasibility of growing Chlorella sp.MUR 269 in biofilm to treat ADFE with high TAN concentrations was investigated. Six different TAN concentrations in ADFE were evaluated for their effects on biofilm growth and nutrient removal by Chlorella sp. MUR 269 using the perfused biofilm technique. Biomass yields and productivities of this alga at various TAN concentrations (mg N NH3 L-1) were 55a (108 g m-2 and 9.80 g m-2 d-1)>100b > 200c = 300c = 500c > 1000d. Growth was inhibited, resulting in a 28% reduction in yield of Chlorella biofilm when this alga was grown at 1000 mg N NH3 L-1. A survey of the photosynthetic parameters reveals evidence of stress occurring in the following sequence: 55 < 100<200 < 300<1000. A significant nutrient removal was observed across various TAN concentrations. The removal pattern also followed the concentration gradients except COD, where the highest removal occurred at 500 mg N NH3 L-1. Higher removal rates were seen at higher nutrient concentrations and declined gradually over time. In general, our results indicated that the perfused biofilm strategy is efficient, minimizes water consumption, offers easy biomass harvesting, and better exposure to light. Therefore, it can be suitable for treating turbid and concentrated effluent with minimal treatment to reduce the TAN concentration.

Nano-clay modified membranes: A promising green strategy for microalgal antifouling filtration.

Membrane fouling is a major challenge which limits the sustainable application of membrane filtration-based microalgal harvesting at industrial level. Membrane fouling leads to increased operational and maintenance costs and represents a major obstacle to microalgal downstream processing. Nano-clays are promising naturally occurring nanoparticles in membrane fabrication due to their low-cost, facile preparation, and their superior properties in terms of surface hydrophilicity, mechanical stability, and resistance against chemicals. The membrane surface modification using nano-clays is a sustainable promising approach to improve membranes mechanical properties and their fouling resistance. However, the positive effects of nano-clay particles on membrane fouling are often limited by aggregation and poor adhesion to the base polymeric matrix. This review surveys the recent efforts to achieve anti-fouling behavior using membrane surface modification with nano-clay fillers. Further, strategies to achieve a better incorporation of nano-clay in the polymer matrix of the membrane are summarised, and the factors that govern the membrane fouling, stability, adhesion, agglomeration and leaching are discussed in depth.

Commercial paper as a promising carrier for biofilm cultivation of Chlorella sp. for the treatment of anaerobic digestate food effluent (ADFE): Effect on the photosynthetic efficiency.

Microalgal technology is still economically unattractive due to the high cost associated with microalgal cultivation and biomass recovery from conventional suspension cultures. Biofilm-based cultivation is a promising alternative for higher biomass yield and cheap/easy biomass harvesting opportunities. Additionally, using anaerobic digestate food effluent (ADFE) as a nutrient source reduces the cultivation cost and achieves ADFE treatment as an added value. However, the search for locally available, inexpensive, and efficient support materials is still open to research. This study evaluates the potential of commercially available, low-cost papers as support material for biofilm cultivation of Chlorella sp. and treatment of ADFE. Among the four papers screened for microalgal attachment, quill board paper performed better in higher biomass yield and stability throughout the study period. The attached growth study was done in a modular food container vessel, using anaerobic digestate food effluent (ADFE) as a nutrient source and a basal medium as a control. The microalgae grew well on the support material with higher biomass yield and productivity of 108.64 g(DW) m-2 and 9.96 g (DW) m-2 d-1, respectively, in the ADFE medium compared with 85.87 g (DW) m-2 and 4.99 g (DW) m-2 d-1, respectively in the basal medium. Chlorophyll, a fluorescence (ChlF) probe, showed that cell density in the biofilm significantly changes the photosynthetic apparatus of the algae, with evidence of stress observed as the culture progressed. Also, efficient nutrient removal from the ADFE medium was achieved in the 100 %, 85 %, and 40.2 % ratios for ammoniacal nitrogen, phosphate, and chemical oxygen demand (COD). Therefore, using quill board paper as carrier material for microalgal cultivation offers promising advantages, including high biomass production, easy biomass harvesting (by scrapping or rolling the biomass with the paper), and efficient effluent treatment.