Clinical Efficacy of Modified Bushen Huoxuetang Combined with LCP in Treatment of Nonunion of Limb Long Bone Shaft Fracture / 中国实验方剂学杂志

Objective:To discuss the clinical efficacy of modified Bushen Huoxuetang combined with autologous bone grafting and locking compression plate （LCP） in treating nonunion of long bone fractures， and the effect on microcirculation， osteogenic differentiation factor and bone metabolism index. Method:A total of 70 patients were randomly divided into control group and observation group by random number table， with 35 cases in each group. Patients in both groups received LCP. Patients in control group got Dieda Shenggu granule， 10 g/time， 1 time/day. Patients in observation group got Bushen Huoxuetang， 1 dose/day. The course of treatment lasted for 3 months， and 3-month follow-up data were recorded. On a weekly basis， the main symptoms， such as pain， tenderness， longitudinal percussion pain and swelling were checked， and the time of disappearing of main symptoms and signs were compared. On a weekly basis， a X-ray examination was performed for callus formation and fracture line， and the fracture healing time was recorded. Before and after treatment， Fugl-Meyer （FMA） was scored， and levels of fibrinogen （FIB）， whole blood viscosity （BV） （high shear， low shear）， plasma viscosity （PV）， platelet aggregation rate （PAR）， <italic>D</italic>-Dimer （<italic>D</italic>-D）， bone morphogenetic protein-2 （BMP-2）， BMP-7， insulin-like growth factor-1 （IGF-1）， vascular endothelial growth factor （VEGF）， transforming growth factor-<italic>β</italic>₁ （TGF-<italic>β</italic>₁）， osteocalcin （BGP）， osteoprotegerin （OPG）， procollagen type Ⅰ N-terminal propeptideserum amino pro peptide （PINP）， serum type 1 collagen cross-linked C-terminal peptide （S-CTX） and serum tartrate resistant acid phosphatase （TRACP） of type I procollagen were detected， and the safety was evaluated. Result:Disappearance time of symptoms and signs and fracture healing time in observation group were all lower than those in control group （<italic>P</italic><0.01）. At the third month after treatment， and during the three-month follow-up， scores of callus and FMA （upper and lower limbs） in observation group were all higher than those in control group （<italic>P</italic><0.01）. Levels of <italic>D</italic>-D， FIB， PAR， BV and PV （high-cut and low-cut）， BMP-2， BMP-7， IGF-1， VEGF， TGF-<italic>β</italic>₁， S-CTX and TRACP were all lower than those in control group （<italic>P</italic><0.01）， whereas levels of BGP， OPG and PINP were higher than those in control group （<italic>P</italic><0.01）. The curative effect of fracture healing was better than that of control group （<italic>Z</italic>=1.977， <italic>P</italic><0.05）. And the limb function recovery was superior to that in control group （<italic>Z</italic>=1.970， <italic>P</italic><0.05）. Conclusion:Based on autogenous bone and LCP， modified Bushen Huoxuetang can promote the fracture healing， shorten the course of disease， and promote the recovery of limb function， with a good clinical efficacy. It can improve microcirculation， promote the expression of osteogenic differentiation factor， regulate bone metabolism， and play a role in promoting fracture healing， with a safety in clinical use.

Clinical Efficacy of Modified Shentong Zhuyutang in Treatment of Acute Discogenic Low Back Pain Due to Wind-Cold and Stasis Obstruction Syndrome / 中国实验方剂学杂志

Objective:To evaluate of modified Shentong Zhuyutang combined with celecoxib capsules in the treatment of acute discogenic low back pain due to wind-cold and stasis obstruction sydrome. Method:One hundred and forty eligible patients were randomly divided into control group （70 cases） and observation group （70 cases）. Patients in both groups took the celecoxib capsules orally， 200 mg/time. On this basis， patients in the control group were further treated with Tongluo Huoxue capsule orally， 6 capsules/time， 3 times/day， while those in the observation group received the modified Shentong Zhuyutang， 1 bag/day， for three weeks. The short-form McGillpain questionnaire （SF-MPQ）， including pain rating index （PRI）， present pain intensity （PPI）， and visual analog scale （VAS）， Oswestry disability index （ODI）， wind-cold obstruction and stasis syndrome， and abilities of daily living （ADL） scoring and the Schober's test were conducted before and after treatment. The tumor necrosis factor-<italic>α</italic> （TNF-<italic>α</italic>）， interleukin-1<italic>β</italic> （IL-1<italic>β</italic>）， IL-6， thromboxane B₂ （TXB₂）， and 6-keto prostaglandin F₁<italic>_α</italic> （6-keto-PGF₁<italic>_α</italic>） levels before and after treatment were measured， followed by the safety evaluation. Result:The PRI， VAS， and PPI scores in SF-MPQ of the observation group were reduced in contrast to those in the control group （<italic>P</italic><0.01）. The wind-cold and stasis obstruction syndrome and ODI scores in the observation group also declined as compared with those of the control group （<italic>P</italic><0.01）， while the ADL and Schober's test scores were elevated （<italic>P</italic><0.01）. The TNF-<italic>α</italic>， IL-1<italic>β</italic>， IL-6， and TXB₂ levels in the observation group were lower than those in the control group （<italic>P</italic><0.01）， whereas the 6-keto-PGF₁<italic>_α</italic> was higher （<italic>P</italic><0.01）. The excellent and good rate of the observation group was （51/63） 80.95%， significantly better than （39/63） 61.90% of the control group （<italic>χ</italic>²＝5.601， <italic>P</italic><0.05）. No adverse reactions occurred after the intake of Chinese medicinal prescriptions. Conclusion:The modified Shentong Zhuyutang combined with celecoxib capsules is efficient and safe in inhibiting inflammation， easing back pain， enhancing lumbar spine mobility， and improving the ADL of patients with acute DLBP of wind-cold obstruction syndrome.

Home range size and habitat use of the blue-crowned laughingthrush during the breeding season.

The home range size and habitat use of the blue-crowned laughingthrush (Garrulax courtoisi, hereafter BCLT), a critically endangered, subtropical, cooperative-breeding bird species in southeast China, were studied during its breeding period using radio telemetry at different sites during 3 consecutive years (2016-18, from May to June of each year). A total of 17 birds (12 males, four females, and one of unknown sex) were tagged, and a total 1515 locations (mean ± se = 89.12 ± 11.42) were obtained over 54 days of tracking. The average 100% minimum convex polygon (MCP) home range size was 10.05 ± 1.17 ha, and the estimated KDE core area (fiexed kernel density estimator, KDE) size was 7.84 ± 1.18 ha. According to the Wilcoxon rank sum tests, both the 100% MCP and KDE core area size of males did not significantly differ from those of females. There were no significant differences in the 100% MCP or KDE core area sizes of the three breeding sites. The available habitats in the breeding sites included water areas, shrubs, grass plots, woodland, residential areas, vegetable field, farmland, and sandy beaches; among them, only woodland was significantly preferred by BCLTs. Woodland (average use ratio was 45.86 ± 1.74%) was strongly preferred by BCLTs for nesting, foraging and roosting. Shrubs/grass plots (24.72 ± 3.39%) and vegetable plots (11.80 ± 1.83%) were used relatively more often than the other habitats, except woodland, since shrubs were always used as perches, and vegetable plots were rich in food resources. Vertically, the canopy layer was used most often from April to June, but it was used most in May when the birds were hatching and brooding. This result indicates that BCLT is predominantly active in the upper strata during the breeding season. In addition, broadleaved trees within or adjacent to villages were important activity areas for the breeding birds; protection and management measures should be increased in these areas.

[A comparative study of efficacy for treating conjunctivochalasis with positioning and quantified patch].

OBJECTIVE: To investigate the efficacy for treating conjunctivochalasis with conjunctival positioning and quantified patch. METHODS: It was a case-control study. A case-control study of 30 patients from the First Hospital of Jiaxing from 2007 to 2008 with binocular conjunctivochalasis. Every patient was treated one eye with simple logadectomy while the other eye with positioning and quantified logadectomy randomly. Subjective and objective evaluations were taken on the 1st, 3rd and 6th months post-operation. Subjective evaluations include effective rate and cure rate. Objective evaluations include slit-lamp biomicroscope morphological variety, tear meniscus height, break-up time (BUT), Schirmer I test (SIT), fluorescein stain test (Fl), the first fluorescein stain. Cure rate and efficacy rate were compared in two groups with chi-square test. RESULTS: Subjective evaluations were on the 1st month post-operation. There were 5 eyes (16.7%) curing, 13 eyes (43.3%) improved, 12 eyes (40.0%) ineffective in simply logadectomy group; While there were 12 eyes (40.0%) curing, 16 eyes (53.3%) improved, 2 eyes (6.7%) ineffective in the positioning and quantified logadectomy group. The effective rate (chi2=9.317, P<0.01) and cure rate(chi2=4.022, P<0.05)had statistic significance. Effective rate in simply logadectomy group was increasing gradually on the 3rd and 6th month post-operation while effective rate in positioning and quantified logadectomy group was stable. But the effective rate in this group was still higher than the simple logadectomy group. To objectively evaluate the cure rate on the 1st month post-operation: slit-lamp biomicroscope morphological variety: there were 10 eyes turn to normal in simply logadectomy group while 29 eyes (chi2=26.447, P<0.01) in the positioning and quantified logadectomy group. Tear meniscus height: there were 18 eyes turn to normal in simply logadectomy group while 30 eyes (chi2=15.000, P<0.01) in the positioning and quantified logadectomy group. BUT: There were 12 eyes turn to normal in simply logadectomy group while 28 eyes (chi2=19.200, P<0.01) in the positioning and quantified logadectomy group. SIT: there were 18 eyes turn to normal in simply logadectomy group while 25 eyes (chi2=4.022, P<0.05) in the positioning and quantified logadectomy group. Fl: there were 20 eyes turn to normal in simply logadectomy group while 28 eyes (chi2=6.667, P<0.05) in the positioning and quantified logadectomy group. The first fluorescein stain: there were 12 eyes turn to normal in simply logadectomy group while 24 eyes (chi2=10.000, P<0.01) in the positioning and quantified logadectomy group. The difference had statistic significance. On the 3rd month post-operation: SIT: there were 18 eyes turn to normal in simply logadectomy group while 19 eyes (chi2=0.071, P>0.05) in the positioning and quantified logadectomy group, without statistic significance. All the other objective evaluations indicatrix had statistic significance. On the 6th month post-operation: SIT: there were 20 eyes turn to normal in simply logadectomy group while 21 eyes (chi2=0.077, P>0.05) in the positioning and quantified logadectomy group, without statistic significance. Fl: there were 23 eyes turn to normal in simply logadectomy group while 28 eyes (chi2=3.268, P>0.05) in the positioning and quantified logadectomy group, without statistic significance. All the other objective evaluations indicatrix had statistic significance. CONCLUSIONS: Positioning and quantified patch resection method not only makes it easier and safer for treating the conjunctivochalasis, but also reduces the complications and creates good result for patients in early period.