Highly efficient porous magnetic polydopamine/copper phosphate with three-dimensional hierarchical nanoflower morphology as a selective platform for recombinant proteins separation.

The separation and purification of recombinant pharmaceutical proteins is a fundamental and challenging step in the biotechnology industry. Hierarchical nanostructures with unique features and high stability can be used as efficient adsorbents. In this study, hierarchical magnetic polydopamine-copper phosphate nanoflowers (Cu-PDA MNFs) were synthesized as high-performance magnetic adsorbents in a simple and low-cost method based on green chemistry. The prepared hybrid Cu-PDA MNFs revealed great performance for separating pure recombinant human growth hormone (rhGH) and the rhGH acquired from recombinant Pichia pastoris yeast fermentation. The analysis confirmed that Cu-PDA MNFs exhibited a high adsorption capacity of 257.4 mg rhGH g-1 Cu-PDA MNFs and a fast adsorption rate for approaching the adsorption equilibrium within less than 30 min with a recovery efficiency of 74% of rhGH from the real sample. In addition, recycling tests demonstrated the stability and recyclability of Cu-PDA MNFs for at least six cycles with almost constant adsorption capacity and no toxicity. Based on these results, Cu-PDA MNFs could be considered as a promising candidate for separation and purification of rhGH. This work presents a new approach to using organic-inorganic nanoflowers as the hierarchical nanostructure for purification of pharmaceutical proteins with high performance.

Mussel-Inspired Magnetic Nanoflowers as an Effective Nanozyme and Antimicrobial Agent for Biosensing and Catalytic Reduction of Organic Dyes.

Mussel-inspired chemistry has been embodied as a method for acquiring multifunctional nanostructures. In this research, a novel mussel-inspired magnetic nanoflower was prepared through a mussel-inspired approach. Herein, magnetic PDA-Cu nanoflowers (NFs) were assembled via incorporating magnetic Fe3O4@SiO2-NH2 core/shell nanoparticles (NPs) into mussel-inspired polydopamine (PDA) and copper phosphate as the organic and inorganic portions, respectively. Accordingly, the flower-like morphology of MNPs PDA-Cu NFs was characterized by scanning electron microscopy (SEM) images. X-ray diffraction (XRD) analysis confirmed the crystalline structure of magnetic nanoparticles (MNPs) and copper phosphate. Vibrating sample magnetometer (VSM) data revealed the superparamagnetic behavior of MNPs (40.5 emu/g) and MNPs PDA-Cu NFs (35.4 emu/g). Catalytic reduction of MNPs PDA-Cu NFs was evaluated through degradation of methylene blue (MB). The reduction of MB pursued the Langmuir-Hinshelwood mechanism and first-order kinetics, in which the apparent reduction rate K app of MB was higher than 1.44 min-1 and the dye degradation ability was 100%. MNPs PDA-Cu NFs also showed outstanding recyclability and reduction efficiency, for at least six cycles. Furthermore, the prepared MNPs PDA-Cu NFs demonstrated a peroxidase-like catalytic activity for catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) to a blue oxidized TMB (oxTMB) solution in the presence of H2O2. Antimicrobial assays for MNPs PDA-Cu and PDA-Cu NFs were conducted on both Gram-negative and Gram-positive bacteria. Moreover, we demonstrated how the existence of magnetic nanoparticles in PDA-Cu NFs influences the inhibition of an increasing zone. Based on the results, mussel-inspired magnetic nanoflowers appear to have great potential applications, including those relevant to biological, catalysis, and environmental research.