RESUMEN
Prangos uloptera DC. (Apiaceae) is indigenous to Iran. It has been used from ancient times for treatment of leukoplakia, digestive disorders and healing scars. In this article, we focus on the various biological screening techniques currently being used to evaluate the biological effects of the plant root extracts. Our results show that dichloromethane (DCM) extract exhibited high-cytotoxic effects on hela cell line, and high antibacterial properties against Staphylococcus aureus and Bacillus subtilis. All of the plant root extracts, n-hexane, DCM and methanol extracts, exhibited modest antioxidant and high-phytotoxic properties. It is assumed that the high biological potential of P. uloptera is related to the presence of coumarins in the plant roots.
Asunto(s)
Apiaceae/química , Extractos Vegetales/química , Extractos Vegetales/farmacología , Plantas Medicinales , Antibacterianos/química , Antibacterianos/farmacología , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/farmacología , Antioxidantes/química , Antioxidantes/farmacología , Bacterias/efectos de los fármacos , Compuestos de Bifenilo , Células HeLa , Humanos , Irán , Dosificación Letal Mediana , Picratos , Raíces de Plantas/químicaRESUMEN
A known furanocoumarin, 8-geranyloxy psoralen, was isolated from n-hexane extract of Prangos uloptera roots by the TLC method. Its structure was determined by comparison of the spectral data with the literature. Cytotoxic effects of the isolated compound were determined by MTT and Tripan blue assays. The antioxidant and antimicrobial potential of the compound were evaluated by DPPH assay and agar dilution method, respectively. The MTT assay results showed that 8-geranyloxy psoralen reduced cell viability of Hela and Mc-Coy cell lines with IC(50) values of 0.792 and 0.835 mM, respectively. Based on the Tripan blue assay, the compound has cytotoxic effects, with an IC(50) value of 1.26 mM for Mc-Coy cell line. The compound exhibited weak antioxidant potential, with an RC(50) value of 0.262 mg mL(-1) and high antimicrobial effects against Staphylococcus epidermidis and Candida kefyr.
Asunto(s)
Apiaceae/química , Furocumarinas/química , Furocumarinas/farmacología , Extractos Vegetales/química , Extractos Vegetales/farmacología , Raíces de Plantas/química , Antiinfecciosos/química , Antiinfecciosos/aislamiento & purificación , Antiinfecciosos/farmacología , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Compuestos de Bifenilo/química , Candida/efectos de los fármacos , Línea Celular , Cromatografía en Capa Delgada , Furocumarinas/aislamiento & purificación , Células HeLa , Hexanos/química , Humanos , Picratos/química , Extractos Vegetales/aislamiento & purificación , Staphylococcus epidermidis/efectos de los fármacosRESUMEN
Cytotoxic, phytotoxic, antimicrobial and antioxidant effects of quercetin 3-O-glucoside (Q3G) isolated by HPLC from aerial parts of Prangos ferulaceae was studied by MTT assay, lettuce germination assay, disk diffusion and DPPH method. Our results showed that Q3G exhibits high antioxidant effect with RC(50) of 22 microg/mL, it has low cytotoxicity and no antibacterial effects. Q3G exhibits high phytotoxic effect with IC(50) value of 282.7 microg/ml, as well. It is assumed that Q3G does not play a defense role in plants and it may act as an allelopatic agent.