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Hum Mutat ; 22(1): 43-50, 2003 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-12815592

RESUMEN

Mutations in the ATM gene are responsible for the autosomal recessive disorder ataxia-telangiectasia (A-T). Many different mutations have been identified using various techniques, with detection efficiencies ranging from 57 to 85%. In this study, we employed short tandem repeat (STR) haplotypes to enhance mutation identification in 55 unrelated A-T families of Iberian origin (20 Spanish, 17 Brazilian, and 18 Hispanic-American); we were able to identify 95% of the expected mutations. Allelic sizes were standardized based on a reference sample (CEPH 1347-2). Subsequent mutation screening was performed by PTT, SSCP, and DHPLC, and abnormal regions were sequenced. Many STR haplotypes were found within each population and six haplotypes were observed across several of these populations. Single nucleotide polymorphism (SNP) haplotypes further suggested that most of these common mutations are ancestrally related, and not hot spots. However, two mutations (8977C>T and 8264_8268delATAAG) may indeed be recurring mutational events. Common haplotypes were present in 13 of 20 Spanish A-T families (65%), in 11 of 17 Brazilian A-T families (65%), and, in contrast, in only eight of 18 Hispanic-American families (44%). Three mutations were identified that would be missed by conventional screening strategies. In all, 62 different mutations (28 not previously reported) were identified and their associated haplotypes defined, thereby establishing a new database for Iberian A-T families, and extending the spectrum of worldwide ATM mutations.


Asunto(s)
Pruebas Genéticas/métodos , Haplotipos/genética , Mutagénesis/genética , Proteínas Serina-Treonina Quinasas/genética , Ataxia Telangiectasia/epidemiología , Ataxia Telangiectasia/etnología , Ataxia Telangiectasia/genética , Proteínas de la Ataxia Telangiectasia Mutada , Brasil/epidemiología , Proteínas de Ciclo Celular , Costa Rica/epidemiología , Proteínas de Unión al ADN , Bases de Datos Genéticas , Efecto Fundador , Hispánicos o Latinos/genética , Humanos , Internet , Fosfatidilinositol 3-Quinasas/genética , Polimorfismo de Nucleótido Simple/genética , Portugal/epidemiología , España/epidemiología , Secuencias Repetidas en Tándem/genética , Proteínas Supresoras de Tumor , Estados Unidos/epidemiología
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