Effects of Tryptophan and Physical Exercise on the Modulation of Mechanical Hypersensitivity in a Fibromyalgia-like Model in Female Rats.

Though the mechanisms are not fully understood, tryptophan (Trp) and physical exercise seem to regulate mechanical hypersensitivity in fibromyalgia. Here, we tested the impact of Trp supplementation and continuous low-intensity aerobic exercise on the modulation of mechanical hypersensitivity in a fibromyalgia-like model induced by acid saline in female rats. Twelve-month-old female Wistar rats were randomly divided into groups: [control (n = 6); acid saline (n = 6); acid saline + exercise (n = 6); acid saline + Trp (n = 6); and acid saline + exercise + Trp (n = 6)]. Hypersensitivity was caused using two intramuscular jabs of acid saline (20 µL; pH 4.0; right gastrocnemius), 3 days apart. The tryptophan-supplemented diet contained 7.6 g/hg of Trp. The three-week exercise consisted of progressive (30-45 min) treadmill running at 50 to 60% intensity, five times (Monday to Friday) per week. We found that acid saline induced contralateral mechanical hypersensitivity without changing the levels of Trp, serotonin (5-HT), and kynurenine (KYN) in the brain. Hypersensitivity was reduced by exercise (~150%), Trp (~67%), and its combination (~160%). The Trp supplementation increased the levels of Trp and KYN in the brain, and the activity of indoleamine 2,3-dioxygenase (IDO), and decreased the ratio 5-HT:KYN. Exercise did not impact the assessed metabolites. Combining the treatments reduced neither hypersensitivity nor the levels of serotonin and Trp in the brain. In conclusion, mechanical hypersensitivity induced by acid saline in a fibromyalgia-like model in female rats is modulated by Trp supplementation, which increases IDO activity and leads to improved Trp metabolism via the KYN pathway. In contrast, physical exercise does not affect mechanical hypersensitivity through brain Trp metabolism via either the KYN or serotonin pathways. Because this is a short study, generalizing its findings warrants caution.

Climate and urbanization drive changes in the habitat suitability of Schistosoma mansoni competent snails in Brazil.

Schistosomiasis is a neglected tropical disease caused by Schistosoma parasites. Schistosoma are obligate parasites of freshwater Biomphalaria and Bulinus snails, thus controlling snail populations is critical to reducing transmission risk. As snails are sensitive to environmental conditions, we expect their distribution is significantly impacted by global change. Here, we used machine learning, remote sensing, and 30 years of snail occurrence records to map the historical and current distribution of forward-transmitting Biomphalaria hosts throughout Brazil. We identified key features influencing the distribution of suitable habitat and determined how Biomphalaria habitat has changed with climate and urbanization over the last three decades. Our models show that climate change has driven broad shifts in snail host range, whereas expansion of urban and peri-urban areas has driven localized increases in habitat suitability. Elucidating change in Biomphalaria distribution-while accounting for non-linearities that are difficult to detect from local case studies-can help inform schistosomiasis control strategies.

Climate and urbanization drive changes in the habitat suitability of Schistosoma mansoni competent snails in Brazil.

Schistosomiasis is a neglected tropical disease caused by Schistosoma parasites. Schistosoma are obligate parasites of freshwater Biomphalaria snails, so controlling snail populations is critical to reducing transmission risk. As snails are sensitive to environmental conditions, we expect their distribution is significantly impacted by global change. Here, we leveraged machine learning, remote sensing, and 30 years of snail occurrence records to map the historical and current distribution of competent Biomphalaria throughout Brazil. We identified key features influencing the distribution of suitable habitat and determined how Biomphalaria habitat has changed with climate and urbanization over the last three decades. Our models show that climate change has driven broad shifts in snail host range, whereas expansion of urban and peri-urban areas has driven localized increases in habitat suitability. Elucidating change in Biomphalaria distribution - while accounting for non-linearities that are difficult to detect from local case studies - can help inform schistosomiasis control strategies.

Trajetorias: a dataset of environmental, epidemiological, and economic indicators for the Brazilian Amazon.

The Trajetorias dataset is a harmonized set of environmental, epidemiological, and poverty indicators for all municipalities of the Brazilian Legal Amazon (BLA). This dataset is the result of a scientific synthesis research initiative conducted by scientists from several natural and social sciences fields, consolidating multidisciplinary indicators into a coherent dataset for integrated and interdisciplinary studies of the Brazilian Amazon. The dataset allows the investigation of the association between the Amazonian agrarian systems and their impacts on environmental and epidemiological changes, furthermore enhancing the possibilities for understanding, in a more integrated and consistent way, the scenarios that affect the Amazonian biome and its inhabitants.

The threshold value for identifying insulin resistance (HOMA-IR) in an admixed adolescent population: A hyperglycemic clamp validated study.

Objective: To validate the homeostasis model assessment (HOMA) of insulin resistance (IR) as a surrogate to the hyperglycemic clamp to measure IR in both pubertal and postpubertal adolescents, and determine the HOMA-IR cutoff values for detecting IR in both pubertal stages. Subjects and methods: The study sample comprised 80 adolescents of both sexes (aged 10-18 years; 37 pubertal), in which IR was assessed with the HOMA-IR and the hyperglycemic clamp. Results: In the multivariable linear regression analysis, adjusted for sex, age, and waist circumference, the HOMA-IR was independently and negatively associated with the clamp-derived insulin sensitivity index in both pubertal (unstandardized coefficient - B = -0.087, 95% confidence interval [CI] = -0.135 to -0.040) and postpubertal (B = -0.101, 95% CI, -0.145 to -0.058) adolescents. Bland-Altman plots showed agreement between the predicted insulin sensitivity index and measured clamp-derived insulin sensitivity index in both pubertal stages (mean =-0.00 for pubertal and postpubertal); all P > 0.05. The HOMA-IR showed a good discriminatory power for detecting IR with an area under the receiver operator characteristic curve of 0.870 (95% CI, 0.718-0.957) in pubertal and 0.861 (95% CI, 0.721-0.947) in postpubertal adolescents; all P < 0.001. The optimal cutoff values of the HOMA-IR for detecting IR were > 3.22 (sensitivity, 85.7; 95% CI, 57.2-98.2; specificity, 82.6; 95% CI, 61.2-95.0) for pubertal and > 2.91 (sensitivity, 63.6; 95% CI, 30.8-89.1, specificity, 93.7; 95%CI, 79.2-99.2) for postpubertal adolescents. Conclusion: The threshold value of the HOMA-IR for identifying insulin resistance was > 3.22 for pubertal and > 2.91 for postpubertal adolescents.

Carnauba wax utilization in salt encapsulation: application in bread.

The present work aims to develop encapsulated NaCl in carnauba wax for bread application, to reduce the salt utilization and assess its impacts on the rheological dough parameters and saltiness perception. Encapsulated salt was obtained blending salt crystals and molten Carnauba wax. Four different bread formulations were produced: 2.0% non-encapsulated salt; 2.0% Encapsulated Salt; 1.5% Encapsulated Salt; 1.0% Encapsulated Salt. Farinograph and alveograph analyses were performed to assess the dough rheology parameters and sensory analysis was conducted to evaluate saltiness. Encapsulation was effective to control Na+ ions release. They vary from 17 to 32 ppm the dissolution of sodium ions in the non-encapsulated and encapsulated samples, respectively. The alveograph and farinograph analyses showed that the 1.5E bread formulation was the closest to the control sample in rheological properties. Finally, a sensory analysis showed no difference in the saltiness perception between control and 1.5% encapsulated salt (4.65 and 4.69 respectively), indicating that carnauba wax encapsulated salt effectively reduced until 35% of salt the bread without changing the saltiness.

Psychotic disorders in the diagnostic stability of a Portuguese ward - Diagnostic retrospective analysis / Trastornos psicóticos en la estabilidad diagnóstica de un barrio portugués - Análisis retrospectivo diagnóstico

ABSTRACT Objectives: Psychiatric diagnosis is based on clinical manifestations, resulting from patients' internal state, their life situation, the evolution of their condition and the response to our interventions. There are currently few objective data which help to establish the diagnosis which is why this is based on diagnostic criteria such as the Diagnostic and Statistical Manual of Mental Disorders (DSM). The DSM defines entities by their diagnostic stability, however there are several causes of variability as categorised by Spritzer et al. (1987): subjects variance (changing in patients), occasions variance (different episodes), information variance (new information) and observation variance (different interpretations). This paper aims to determine the diagnostic stability of patients with Psychotic Disorders among patients readmitted to our Psychiatric Unit. Methods: Retrospective analysis of the diagnoses of patients with Psychotic Disorders who had been readmitted to our unit. We analysed data from the last 12 years - 5422 admission episodes with 507 patients with a relevant diagnosis in this period. Results: Psychiatric diagnosis does evolve over time, nevertheless some diagnostic groups show a relatively significant stability over time - Bipolar Disorder and Schizophrenia with 69% and 77% stability, respectively. Diagnosis such as Depressive Psychosis and Drug-induced psychosis show a significantly lower stability (8% and 21%, respectively). Conclusions: Knowing our own reality can make us aware that a cross-sectional view of patients can be insufficient and only time can determine a clear diagnosis. This study may help us to understand how psychotic disorders evolve.

RESUMEN Objetivos: El diagnóstico psiquiátrico se basa en las manifestaciones clínicas, consecuencia del estado interno del paciente, de su situación vivencial, de la evolución de su enfermedad y de la respuesta a nuestras intervenciones. Actualmente, existen pocos datos objetivos que ayudan a establecer el diagnóstico por lo que éste se basa en criterios diagnósticos como el Manual Diagnóstico y Estadístico de los Trastornos Mentales (DSM). La DSM define diagnósticos por su estabilidad, sin embargo, existen varias causas de variabilidad caracterizadas por Spritzer et al. (1987): variación en el sujeto (cambio en el paciente), variación ocasional (diferentes episodios), variación en la información (nueva información) y variación en la observación (diferentes interpretaciones). Este trabajo pretende evaluar la estabilidad diagnóstica de los pacientes con Trastornos Psicóticos reinternados en nuestro internamiento psiquiátrico. Métodos: Análisis retrospectivo de los diagnósticos de los pacientes con Trastornos Psicóticos con reingresos en nuestro servicio. Se analizaron datos de los últimos 12 anos - 5422 admisiones con 507 pacientes con diagnóstico de interés en ese período. Resultados: El diagnóstico psiquiátrico se altera con el tiempo, sin embargo, algunos grupos revelan una mayor estabilidad a lo largo del tiempo - Perturbación Afectiva Bipolar y Esquizofrenia con el 69% y el 77%, respectivamente. Los diagnósticos como la depresión psicótica y la psicosis tóxica, revelan una estabilidad significativamente menor (8% y 21% respectivamente). Conclusiones: Conocer nuestra realidad nos hace conscientes de que una mirada transversal a los enfermos puede ser insuficiente y sólo el tiempo puede determinar un diagnóstico claro. Este trabajo puede ayudarnos a entender cómo evolucionan las enfermedades psicóticas.

Epidemiology, Biodiversity, and Technological Trajectories in the Brazilian Amazon: From Malaria to COVID-19.

The Amazon biome is under severe threat due to increasing deforestation rates and loss of biodiversity and ecosystem services while sustaining a high burden of neglected tropical diseases. Approximately two thirds of this biome are located within Brazilian territory. There, socio-economic and environmental landscape transformations are linked to the regional agrarian economy dynamics, which has developed into six techno-productive trajectories (TTs). These TTs are the product of the historical interaction between Peasant and Farmer and Rancher practices, technologies and rationalities. This article investigates the distribution of the dominant Brazilian Amazon TTs and their association with environmental degradation and vulnerability to neglected tropical diseases. The goal is to provide a framework for the joint debate of the local economic, environmental and health dimensions. We calculated the dominant TT for each municipality in 2017. Peasant trajectories (TT1, TT2, and TT3) are dominant in ca. fifty percent of the Amazon territory, mostly concentrated in areas covered by continuous forest where malaria is an important morbidity and mortality cause. Cattle raising trajectories are associated with higher deforestation rates. Meanwhile, Farmer and Rancher economies are becoming dominant trajectories, comprising large scale cattle and grain production. These trajectories are associated with rapid biodiversity loss and a high prevalence of neglected tropical diseases, such as leishmaniasis, Aedes-borne diseases and Chagas disease. Overall, these results defy simplistic views that the dominant development trajectory for the Amazon will optimize economic, health and environmental indicators. This approach lays the groundwork for a more integrated narrative consistent with the economic history of the Brazilian Amazon.

Brazilian Consortium for the Study on Renal Diseases Associated With COVID-19: A Multicentric Effort to Understand SARS-CoV-2-Related Nephropathy.

Kidney involvement appears to be frequent in coronavirus disease 2019 (COVID-19). Despite this, information concerning renal involvement in COVID-19 is still scarce. Several mechanisms appear to be involved in the complex relationship between the virus and the kidney. Also, different morphological patterns have been described in the kidneys of patients with COVID-19. For some authors, however, this association may be just a coincidence. To investigate this issue, we propose assessing renal morphology associated with COVID-19 at the renal pathology reference center of federal university hospitals in Brazil. Data will come from a consortium involving 17 federal university hospitals belonging to Empresa Brasileira de Serviços Hospitalares (EBSERH) network, as well as some state hospitals and an autopsy center. All biopsies will be sent to the referral center for renal pathology of the EBSERH network. The data will include patients who had coronavirus disease, both alive and deceased, with or without pre-existing kidney disease. Kidney biopsies will be analyzed by light, fluorescence, and electron microscopy. Furthermore, immunohistochemical (IHC) staining for various inflammatory cells (i.e., cells expressing CD3, CD20, CD4, CD8, CD138, CD68, and CD57) as well as angiotensin-converting enzyme 2 (ACE2) will be performed on paraffinized tissue sections. In addition to ultrastructural assays, in situ hybridization (ISH), IHC and reverse transcription-polymerase chain reaction (RT-PCR) will be used to detect Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) in renal tissue. For the patients diagnosed with Collapsing Glomerulopathy, peripheral blood will be collected for apolipoprotein L-1 (APOL1) genotyping. For patients with thrombotic microangiopathy, thrombospondin type 1 motif, member 13 (ADAMTS13), antiphospholipid, and complement panel will be performed. The setting of this study is Brazil, which is second behind the United States in highest confirmed cases and deaths. With this complete approach, we hope to help define the spectrum and impact, whether immediate or long-term, of kidney injury caused by SARS-CoV-2.

Development, environmental degradation, and disease spread in the Brazilian Amazon.

The Amazon is Brazil's greatest natural resource and invaluable to the rest of the world as a buffer against climate change. The recent election of Brazil's president brought disputes over development plans for the region back into the spotlight. Historically, the development model for the Amazon has focused on exploitation of natural resources, resulting in environmental degradation, particularly deforestation. Although considerable attention has focused on the long-term global cost of "losing the Amazon," too little attention has focused on the emergence and reemergence of vector-borne diseases that directly impact the local population, with spillover effects to other neighboring areas. We discuss the impact of Amazon development models on human health, with a focus on vector-borne disease risk. We outline policy actions that could mitigate these negative impacts while creating opportunities for environmentally sensitive economic activities.

In vitro efficacy of Metarhizium anisopliae sensu lato against unfed Amblyomma parvum (Acari: Ixodidae).

Amblyomma parvum Aragão (Acari: Ixodidae) is a tick species found with wide distribution in the Neotropical region. Even though it is a wildlife-related tick, it is also a frequent parasite of domestic animals, is aggressive to human beings and may harbor pathogenic microorganisms. Therefore, it is a target species for control on domestic animals, particularly those at the rural-wildlife interface. Herein, the efficacy of two isolates (E9 and IBCB 425) of an entomopathogenic fungus, Metarhizium anisopliae sensu lato, already evaluated for ticks that parasitize domestic animals, was tested against unfed A. parvum adults. Both isolates displayed high acaricidal efficacy after immersion in fungal conidial suspensions for 5 min. Isolate E9 killed all ticks by the 7th day post-treatment, and isolate IBCB 425 did so by the 11th day. Tick mortality of 80 and 90% was achieved as early as the 3rd and 4th days, respectively, with both treatments. Thus, if a commercial M. anisopliae s.l. acaricide against domestic animal ticks is developed, it would also be effective against A. parvum.

The Urban Gradient in Malaria-Endemic Municipalities in Acre: Revisiting the Role of Locality.

Urbanization has altered the distribution of diseases of public health importance along gradients of human occupation. Adopting dichotomous urban/rural categories to explain differences in the risk of exposure, as well as the prevention of diseases is insufficient. In this paper, we present the potential of representations based on the gradient perspective to characterize the living spaces of municipalities where malaria is endemic in northwest Acre. Inventoried data in 40 localities in the Mâncio Lima and Rodrigues Alves municipalities and information on land use and land cover obtained from the TerraClass Database were used to characterize the urban spatial forms and their social content. Results showed a gradient of intensities: from municipal seats to the most connected localities through the road network to riverine communities. Based on the results, we hope to advance the discussion about the use of normative definitions of “urban” and “rural” for public policies and actions to control and eliminate malaria, considering the position of each locality in its own locally referenced urban gradient.

Mixoma auricular: uma causa rara de acidente vascular cerebral isquêmico / Atrial myxoma: a rare cause of ischemic stroke

O mixoma auricular é uma causa rara de acidente vascular cerebral isquêmico, sendo mais frequente em mulheres jovens. Sua etiologia é desconhecida, e as manifestações clínicas ocorrem por obstrução valvular, embolismo ou sintomas constitucionais. Descrevemos o caso de uma mulher de 62 anos com hemiparesia esquerda e ataxia da marcha, com evidência de lesões isquêmicas bilaterais na ressonância magnética craniencefálica. No ecocardiograma, identificou-se massa auricular esquerda sugestiva de mixoma, submetida à ressecção cirúrgica e confirmação histológica. Posteriormente, foi diagnosticada fibrilação auricular, sendo esta a complicação pós-operatória mais comum. Na suspeita de causa cardiogênica, o ecocardiograma deve ser prontamente realizado, pois o mixoma é potencialmente tratável e tem baixo risco de recorrência após a ressecção cirúrgica. Este caso realça a importância do estudo complementar no acidente vascular cerebral isquêmico e a necessidade de excluir uma fonte embólica proximal na presença de isquemia cerebral em territórios vasculares diferentes.(AU)

The atrial myxoma is a rare cause of ischemic stroke, and is more common in young women. Its etiology is unknown, and clinical manifestations occur due to valve obstruction, embolism or constitutional symptoms. We describe the case of a 62-year-old woman with left hemiparesis and gait ataxia, with evidence of bilateral ischemic lesions on brain magnetic resonance imaging. On the echocardiogram, a left atrial mass suggestive of myxoma was identified; it was resected, and histologically confirmed. Later, an atrial fibrillation was diagnosed, which is the most common postoperative complication. On suspicion of a cardiogenic cause, the echocardiogram should be promptly performed because the myxoma is potentially treatable with a low risk of recurrence after surgical resection. This case highlights the importance of complementary study in ischemic stroke, and the need to rule out a proximal embolic source in the presence of cerebral ischemia in different vascular territories.(AU)

Os processos de urbanização e conversão florestal na Amazônia paraense ­ um estudo multiescalar / The urbanization and forest conversion processes in the Paraense Amazon ­ a multiescalar study / Los procesos de urbanización y conversión selvática en la Amazonia Paraenanse ­ un estudio multiescalar

Resumo Na Amazônia brasileira, as dinâmicas de urbanização e conversão florestal possuem lógicas complexas, dependentes de fatores e agentes atuando em diferentes escalas. Este artigo explora a evolução espacial e temporal da urbanização e da conversão florestal na Amazônia, a partir de elementos mensuráveis presentes nestes processos: a expansão urbana, dimensão observável dos processos de urbanização; e o desmatamento, uma medida dos processos de conversão florestal. A identificação de padrões espaço-temporais similares, avaliados quanto às tendências de evolução temporal do grau de urbanização e incremento de desmatamento em 2000, 2010 e 2014, foi base para a proposta de uma tipologia das relações entre expansão urbana e desmatamento para os estados da Amazônia Legal e municípios do Pará. O estudo explorou também as relações entre expansão urbana e desmatamento, com uso de regressões geograficamente ponderadas (GWR), observando duas unidades espaciais de análise para o estado do Pará: os limites municipais e uma grade celular (20x20km). Esta abordagem multiescalar evidenciou a dificuldade de se estabelecerem relações conclusivas quanto à co-ocorrência, ou concordância, da evolução dos processos de urbanização e conversão florestal. Os resultados contribuem para o estabelecimento de uma agenda sobre modelos de desenvolvimento regional que retome o urbano como essencial para uma Amazônia socialmente justa e ambientalmente responsável.

Abstract In the Brazilian Amazon, dynamics of urbanization and forest conversion have complex logics, and are dependent on factors and agents operating at different levels. This article explores the spatial and temporal evolution of urbanization and forest conversion in the Amazon region from measurable elements present in these processes: urban expansion, observable dimensions of urbanization processes, and deforestation, a measure of forest conversion processes. The identification of similar spatiotemporal patterns, evaluated according to trends in the evolution of the degree of urbanization and the increase of deforestation in the years 2000, 2010 and 2014, was the basis for proposing a typology of relation between urban expansion and deforestation for the states of the Brazilian Legal Amazon and municipalities of Pará State. The study also explored the relation between urban expansion and deforestation, using Geographically Weighted Regression (GWR), observing two spatial units of analysis for Pará State: the municipal boundaries and a regular grid (20x20km). This multiscale approach evidenced the difficulty in establishing conclusive relationships regarding co-occurrence, or agreement, between the evolution of urbanization and forest conversion processes. Our results aim to establish an agenda on regional development models which take the urban phenomenon as essential for a socially just and environmentally responsible Amazon.

Resumen En la Amazonia brasileña las dinámicas de urbanización y conversión selvática poseen lógicas complejas que dependen de factores y agentes que actúan en diferentes escalas. Este artículo explora la evolución espacial y temporal de la urbanización y la conversión selvática en la Amazonia a partir de elementos mensurables presentes en estos procesos: la expansión urbana, dimensión observable de los procesos de urbanización, y la deforestación, una medida de los procesos de conversión selvática. La identificación de patrones espaciotemporales similares, evaluados en cuanto a las tendencias de evolución temporal del grado de urbanización y del incremento de deforestación en los años 2000, 2010 y 2014 fueron la base para la propuesta de una tipología de las relaciones entre expansión urbana y deforestación para los municipios del Pará. El estudio exploró también las relaciones entre expansión urbana y deforestación, mediante el uso de la regresión geográficamente ponderada (GWR), observando dos unidades espaciales de análisis para el Pará: los límites municipales y una rejilla con celdas (20x20km). Este enfoque multiescalar evidenció la dificultad para establecer relaciones concluyentes en cuanto a la coocurrencia, o la concordancia, entre los procesos de urbanización y conversión selvática. Los resultados contribuyen al establecimiento de una agenda sobre modelos de desarrollo regional que retome lo urbano como esencial para una Amazonia socialmente justa y ambientalmente responsable.

Compatibility of Metarhizium anisopliae with liposoluble photoprotectants and protective effect evaluation against solar radiation / Compatibilidade de Metarhizium anisopliae com fotoprotetores lipossolúveis e avaliação do efeito protetor contra a radiação solar

The aim of this study was to analyze the compatibility of the Metarhizium anisopliae JAB 68 isolate with liposoluble photoprotectants and the photoprotection conferred to its conidia. The fungus was cultivated in a medium with the photoprotectants Neo Heliopan® BB, Neo Heliopan® E 1000, Eusolex® 6007 and Neo Heliopan® AV in various concentrations. Vegetative growth, sporulation and germination were assessed to determine compatibility. Then, conidia were suspended in oil containing the compatible photoprotectants, in their respective concentrations, and exposed to 0-7 hours of light from a solar simulator operating at 1,000 W m-2. Neo Heliopan BB® and Neo Heliopan® E 1000 were compatible with M. anisopliae at 2% and 1%, respectively. Eusolex® 6007 and Neo Heliopan® AV were compatible up to 8% a 7%, respectively. Conidia exposed to radiation from the solar simulator, with photoprotectant-free oil as its carrier, presented germination of 78.12% after five hours. When adding any photoprotectant to the conidia oily suspension, the germination percentage was always higher than that obtained in the control. Neo Heliopan® BB at 1 and 2% and Neo Heliopan® AV at 0.5% increased the germination. After 7 hours of exposure the germination percentages were 93.77, 94.55 and 98.82%, respectively. Eusolex® 6007 was not as efficient as previous products, but in the same exposure period was able to protect the conidia (88.60% germination) when used at a 1% concentration. Adding photoprotectants in oily formulations of M. anisopliae protects against the harmful effects of UV radiation contributing to the fungus efficiency in the field.

O objetivo desse trabalho foi analisar a compatibilidade do isolado JAB 68 de Metarhizium anisopliae com fotoprotetores lipossolúveis e a fotoproteção conferida aos seus conídios. O fungo foi cultivado em meio contendo os fotoprotetores Neo Heliopan® BB, Neo Heliopan® E 1000, Eusolex® 6007, Neo Heliopan® AV em várias concentrações. O crescimento vegetativo, a esporulação e a germinação foram avaliados para determinar a compatibilidade. Conídios foram suspensos em óleo contendo os fotoprotetores compatíveis, em suas respectivas concentrações, e submetidos por 0 a 7 horas à luz de um simulador solar na potência de 1.000W m-2. Neo Heliopan BB® e Neo Heliopan® E 1000 foram compatíveis com M. anisopliae à 2% e 1%, respectivamente. Eusolex® 6007 e Neo Heliopan® AV foram compatíveis até 8% e 7%, respectivamente. Conídios de M. anisopliae expostos à radiação do simulador solar, tendo óleo isento de fotoprotetor como veículo, apresentaram germinação de 78,12% após cinco horas. Adicionando-se qualquer fotoprotetor à suspensão oleosa de conídios a porcentagem de germinação foi sempre maior que a obtida no controle. Neo Heliopan® BB a 1 e 2% e Neo Heliopan® AV a 0,5% incrementaram a germinação. Após 7 horas de exposição as porcentagens de germinação foram de 93,77, 94,55 e 98,82%, respectivamente. Eusolex® 6007 não foi tão eficiente quanto os produtos anteriores, mas no mesmo período de exposição foi capaz de proteger os conídios (88,60% de germinação) quando usado na concentração de 1%. A adição de fotoprotetores em formulações oleosas de M. anisopliae confere proteção aos raios UV, contribuindo com a eficiência do fungo no campo.

Demografia, planejamento territorial e a questão habitacional: prognóstico da situação habitacional do Litoral Norte Paulista / Demography, territorial planning and housing: prognosis of the housing situation in the North Coast of São Paulo / Demografía, planificación territorial y vivienda: prognosis de la situación de la vivienda en la Costa Norte de São Paulo

Técnicas de análise demográfica são indispensáveis para o planejamento urbano, pois mudanças nos regimes populacionais se relacionam diretamente à produção e reprodução do espaço nas cidades. A questão habitacional é central nessa relação, já que o ambiente construído é predominantemente composto de residências. Todavia esses temas ainda não têm sido frequentemente abordados de forma conjunta. O objetivo desse artigo é discutir tais relações e mostrar, em um exercício prático de prognóstico para a região do Litoral Norte Paulista, como técnicas de projeção da demanda futura por habitação podem ser úteis às análises territoriais. Observam-se uma tendência de envelhecimento da população e o aumento do número de mulheres nos municípios do Litoral Norte, o que significa que grupos com autonomia para constituir um novo domicílio tendem a aumentar em proporção. Os resultados da projeção da demanda futura por habitação refletem este aumento, que terá influência direta na ocupação do território.

Demographic analysis techniques are indispensable for urban planning because changes in population regimes are directly related to the production and reproduction of space in cities. The housing issue is central in this relationship because the man-made environment is predominantly composed by residences. However, these themes have not been tackled together yet. The objective of this article is to discuss these relationships and to show, in a practical exercise of prognosis for the region of the North Coast of São Paulo, how techniques for projecting future housing demands can be useful for territorial analyzes. There is a trend towards an aging population and an increase in the number of women in these towns, it means that groups that have the autonomy to establish a new home tend to increase in proportion. The results of future demand for housing projections reflect this increase and will have a direct influence on the occupation of the territory.

Las técnicas de análisis demográfico son indispensables para la planificación urbana porque los cambios en los regímenes de población se relacionan directamente con la producción y reproducción del espacio en las ciudades. La vivienda es uma cuestión central en esta relación, ya que el entorno construido está compuesto principalmente por residencias. Sin embargo, estos temas aún no han sido abordados conjuntamente. El objetivo de este artículo es discutir estas relaciones y mostrar, en un ejercicio práctico de pronóstico para la región de la costa norte de São Paulo, cómo las técnicas para proyectar la demanda futura de vivienda pueden ser útiles para los análisis territoriales. Hay una tendencia hacia un envejecimiento de la población y un aumento en el número de mujeres en este tipo de ciudades, lo que significa que grupos que tienen autonomía para establecer un nuevo hogar tienden a aumentar en proporción. Los resultados de la proyección de la demanda futura de vivienda reflejan este aumento, que tendrá una influencia directa en la ocupación del territorio.

An observatory to gather and disseminate information on the health-related effects of environmental and climate change.

This report sought to critically examine proposals, potentials, and challenges of environmental health observatories with an emphasis on climate change processes. A critical review of existing environmental health observatories was performed, examining their purposes, potential audiences, and technological platforms. The implementation of the Brazilian Climate and Health Observatory (C&HO) is described, and two stages are defined: (i) the requirement analysis and negotiation stage that identified the national and regional institutional players and their roles as data producers/users; and (ii) thematic health-related workshops that reviewed water-related diseases, vector-borne diseases, extreme climate events, and health problems derived from forest fires. The C&HO is an example of making information on climate and health available through an Internet site where data from different origins can be accessed on a common platform. Complex queries are made by users and can be executed over multiple sites, geographically distributed, with all technical details hidden from the end user. At this stage of the C&HO prototype, alongside the queries, users can also produce semi-qualitative graphs and maps. A multi-scale approach was developed using the platform by setting up sentinel sites. Building a successful observatory is a participatory process that involves choosing indicators, data sources, information technology, and languages to best reach different audiences, such as researchers, citizens, public health professionals, and decisionmakers.

An observatory to gather and disseminate information on the health-related effects of environmental and climate change / Un observatorio para recopilar y difundir información sobre los efectos del cambio ambiental y climático relacionados con la salud

This report sought to critically examine proposals, potentials, and challenges of environmental health observatories with an emphasis on climate change processes. A critical review of existing environmental health observatories was performed, examining their purposes, potential audiences, and technological platforms. The implementation of the Brazilian Climate and Health Observatory (C&HO) is described, and two stages are defined: (i) the requirement analysis and negotiation stage that identified the national and regional institutional players and their roles as data producers/users; and (ii) thematic health-related workshops that reviewed water-related diseases, vector-borne diseases, extreme climate events, and health problems derived from forest fires. The C&HO is an example of making information on climate and health available through an Internet site where data from different origins can be accessed on a common platform. Complex queries are made by users and can be executed over multiple sites, geographically distributed, with all technical details hidden from the end user. At this stage of the C&HO prototype, alongside the queries, users can also produce semi-qualitative graphs and maps. A multi-scale approach was developed using the platform by setting up sentinel sites. Building a successful observatory is a participatory process that involves choosing indicators, data sources, information technology, and languages to best reach different audiences, such as researchers, citizens, public health professionals, and decisionmakers.

Este informe tiene el propósito de examinar críticamente las propuestas, las posibilidades y los retos de los observatorios de salud ambiental con especial interés en los procesos del cambio climático. Se realizó un examen crítico de los observatorios de salud ambiental existentes, para lo cual se evaluó su finalidad, los posibles destinatarios y las plataformas tecnológicas. En este informe se describe la ejecución del Observatorio Nacional de Clima y Salud del Brasil dividida en dos fases: 1) la fase de análisis y negociación de los requisitos, cuyo fin fue definir las instituciones nacionales y regionales que tendrían a su cargo la producción y el aprovechamiento de los datos; y 2) los talleres celebrados sobre temas de salud acerca de las enfermedades relacionadas con el agua, las enfermedades transmitidas por vectores, los acontecimientos climáticos extremos y los problemas de salud derivados de los incendios forestales. El Observatorio es un ejemplo de plataforma de internet que ofrece información y datos sobre el clima y la salud provenientes de diferentes fuentes. Los usuarios pueden plantear consultas complejas en varios sitios, localizados en distintas zonas geográficas y todos los detalles técnicos se mantienen ocultos para el usuario. En esta fase de prototipo del Observatorio es posible generar gráficos y mapas semicualitativos junto a las consultas. Además, la plataforma permitió generar una estrategia de varias escalas a través del establecimiento de centros centinela. La creación de un observatorio eficaz es un proceso participativo que comprende la elección de los indicadores, las fuentes de los datos, la tecnología de la información y los idiomas que serán empleados con el propósito de llegar a diferentes públicos, como investigadores, ciudadanos, profesionales de la salud pública y encargados de adoptar las decisiones.