RESUMEN
EEF1D (eukaryotic translation elongation factor 1δ) is a subunit of the elongation factor 1 complex of proteins that mediates the elongation process during protein synthesis via enzymatic delivery of aminoacyl-tRNAs to the ribosome. Although the functions of EEF1D in the translation process are recognized, EEF1D expression was found to be unbalanced in tumours. In the present study, we demonstrate the overexpression of EEF1D in OSCC (oral squamous cell carcinoma), and revealed that EEF1D and protein interaction partners promote the activation of cyclin D1 and vimentin proteins. EEF1D knockdown in OSCC reduced cell proliferation and induced EMT (epithelial-mesenchymal transition) phenotypes, including cell invasion. Taken together, these results define EEF1D as a critical inducer of OSCC proliferation and EMT.
Asunto(s)
Carcinoma de Células Escamosas/genética , Proliferación Celular/genética , Transición Epitelial-Mesenquimal/genética , Regulación Neoplásica de la Expresión Génica/genética , Neoplasias de Cabeza y Cuello/genética , Neoplasias de la Boca/genética , Factor 1 de Elongación Peptídica/genética , Carcinoma de Células Escamosas/diagnóstico , Línea Celular Tumoral , Movimiento Celular/genética , Neoplasias de Cabeza y Cuello/diagnóstico , Humanos , Neoplasias de la Boca/diagnóstico , Neoplasias de la Boca/metabolismo , Neoplasias de la Boca/patología , Fenotipo , Carcinoma de Células Escamosas de Cabeza y CuelloRESUMEN
O estudo comparou in vitro a resistência à fratura em dentes tratados endodonticamente reforçados com pinos intra-radiculares pré-fabricados, utilizando diferentes agentes cimentantes. Cinquenta dentes humanos permanentes unirradiculares obturados, foram divididos em 5 grupos: G I-controle (sem pino); G II- pino de fibra de carbono + cimento Rely X; G III- pino de fibra de carbono + cimento Enforce; G IV- pino de fibra de vidro + cimento Rely X; G V- pino de fibra de vidro + cimento En-force. Após cimentação dos pinos e restauração coronal, as raízes foram incluídas em resina acrílica quimicamente ativada e submetidos à carga de compressão em uma Máquina Universal de Ensaios (Instron 5582). Os dados obtidos foram analisados estatisticamente pelos testes Exato de Fisher e o teste F (ANOVA), a um nível de signifcância de 5,0%, os quais demonstraram que em relação ao local da interface no grupo total, a maioria (67,5%) das amostras teve fratura na interface dente/agente cimentante; destacou-se também que a freqüência de fraturas no dente/agente cimentante variou de 4 (grupo V) a 10 (grupo III), diferenças estas significante entre os grupos. Concluiu-se que o uso dos pinos pré-fabricados não proporcionou aumento na resistência dos dentes tratados endodonticamente; o pino de fibra de vidro em associação ao sistema Enforce mostrou-se superior na resistência à fratura, sendo estatisticamente signifcante quando comparado ao grupo Enforce / pino de fibra de carbono; em relação à resistência de união, o maior comprometimento se deu na interface agente cimentante/dente quando comparada ao agente cimentante/pino.
The study compared in vitro the resistance to fracture of teeth endodontically treated reinforced with prefabricated intra-radicular posts, cemented with diferent cementation agents. Fifty permanents human teeth which had only one root was filled and divided in five groups: G I- control (without pin); G II carbon-fiber post + Rely X cement; G III carbon-fiber post + Enforce cement; G- IV fiber core post + Rely-X cement; G-V f- ber core post + Enforce cement. After cementation of this posts and coronal restoration, the root was included in self-activated acrylic resin and was submited to compressive axial loading in a Universal Testing Machine (Instron 5582). The results were statistically analyzed by Exato de Fisher test and test F (ANOVA), with 5,0 % signifcance, which demonstrated that in relation to the interface place in the total group, the majority (67,5%) of the samples had fracture on interface tooth/cementation agent ; the frequency of fractures on teeth/cementation agent varied from 4 (group V) to 10 (group III), and these diferences were significant among the groups. It was concluded that the use of prefabricated intra radicular posts couldn´t intensify the resistance of endodontically treated teeth; fiber core post associated to Enforce cement was more resistant to fracture, and statistically significant than carbon-fiber post and Enforce cement group. About bond strength the interface cement agent/teeth was less resistant than cement agent/pin.
