Micobacteriosis sistémica por mycobacterium avium en paciente con SIDA / Systemic mycobacteriosis for Mycobacterium avium in a AIDS patient

Se analizaron repetidas muestras procedentes de un paciente cubano con SIDA, para descartar la presencia de bacilos ácido alcohol resistente (BAAR), pasadas 2-3 semana en las muestras de esputo, líquido cefalorraquídeo y de hemocultivo que habían sido procesadas y cultivadas se detecta la presencia de algunas colonias, como resultado se obtuvo el aislamiento de una cepa micobacteriana no pigmentada, de crecimiento lento perteneciente al Grupo III de Runyon, esta cepas fueron clasificadas como Mycobacterium avium por los métodos convencionales establecidos para la identificación de cepas micobacterianas, como técnica confirmativa diagnóstica se utilizó el análisis de las fracciones de ácido micólicos por la técnica de cromatografía en capa delgada bidimensional. El objetivo fundamental de este estudio ha sido reportar el primer caso de micobacteriosis sistémica en un paciente cubano con SIDA

Several sputum and blood culture simples from a Cuban HIV/AIDS patient were analyzed to discard the presence of alcohol acid resistant bacillus. After 2-3 weeks the culture revealed in both kinds of samples some colonies from non-pigmented mycobacterium strain with slow growth and belonging to III Runyon Group. This strain was classified as Mycobacterium avium by conventional methods established for mycobacterium identification. To diagnostic confirmative method was used the analysis of fraction mycolic acid by bi-dimensional thin layer chromatography. The main objective of this study was to report the first case of systemic mycobacteriosis in a Cuban HIV/AIDS patient

[Evaluation of the pyrazinamidase test for the detection of susceptibility of Mycobacterium tuberculosis to pyrazinamide]. / Evaluación de la prueba de la pirazinamidasa para la determinación de la susceptibilidad de Mycobacterium tuberculosis a la pirazinamida.

The pyrazinamidase test was used to determine susceptibility to pyrazinamide in 55 strains of Mycobacterium tuberculosis received at the National Laboratory of Reference and Research of Tuberculosis and Mycobacteria of the "Pedro Kourí" Institute of Tropical Medicine during the first semester of 1997. On comparing the results obtained with the method of proportions, it was attained a correlation of 83.6% of sensitivity with 16.4% of false positives. It was proved that the pyrazinamidase test should not replace the method of proportions.

[The identification of Mycobacterium tuberculosis strains using paper strips for the niacin technic]. / Identificación de cepas de Mycobacterium tuberculosis utilizando tiras de papel para la técnica de niacina.

Mycobacterium tuberculosis strains were identified using paper strips for niacin technique; these paper strips were made at the National Reference Laboratory on Tuberculosis and Mycobacteria of the IPK. Results were compared with the conventional niacin technique and a 100% of coincidence was obtained; advantages, sensitivity and firmness of this method are reported.

[Mycobacterium fortuitum: the determination of its susceptibility by the disk diffusion technic]. / Mycobacterium fortuitum: determinación de la susceptibilidad mediante la técnica de difusión por disco.

A study was carried out on 40 Mycobacterium fortuitum strains isolated from 39 symptomatic respiratory patients and 1 from a chronic skin ulcer, the susceptibility of whom to different antimicrobial agents was determined by the disk diffusion method. The strain showed sensitivity to aminoglucosides such as amikacin, gentamicin and kanamycin, and in all cases resistance to the penicillins ans cephalosporins used.

[Sensitivity of Mycobacterium fortuitum by the broth microdilution method determining the inhibitory minimal concentration]. / Estudio de la sensibilidad de Mycobacterium fortuitum por el método de la microdilución en caldo determinando la concentración mínima inhibitoria.

A study was conducted on 40 Mycobacterium fortuitum strains isolated from symptomatic patients suffering from respiratory diseases and skin lesions. Their susceptibility to different antimicrobial agents was determined by the broth microdilution method, measuring minimal inhibitory concentration. There was sensitivity of the strains to gentamicin and tetracycline as well as resistance to the tuberculostatic drugs used (isoniazid and ethambutol) and cefalotin.

[Non-tuberculous mycobacteria isolated in Cuba during the 1985-1989 period. Preliminary report]. / Estudio de micobacterias no tuberculosas aisladas en Cuba durante el período 1985-1989. Reporte preliminar.

A number of 1,061 strains of nontuberculous mycobacteria referred to "Pedro Kouri" Institute of Tropical Medicine during the period of 1985-1989 were studied. Strains were from Provincial Centers of Hygiene and Epidemiology of the country. According to the results obtained, most of the strains classified are found in groups III and IV according to the criteria of Runyon (54, 76, and 36%, respectively). Species with a greater frequency belong to the complex MAI and M. fortuitum.