Comparison between the traditional Manta net and an innovative device for microplastic sampling in surface marine waters.

Manta nets are commonly used for microplastics sampling although a number of limitations have emerged. In this study we compare the manta net to an innovative microplastic sampler, referred to as MuMi, registered as utility model. The results highlight the large variability that can exist in the outcomes of the different studies due to the lack of harmonization between methods and the differing factors such as sampling mesh size, representativeness or reproducibility of the sampling volumes. Control over the filtered volume is an issue to be improved in trawl sampling methods, while in the MuMi sampler the control over the sampling depth could be improved. Still, MuMi represents a highly advantageous sampling system in terms of ease of operation, lower cost, smaller microplastics target size and greater precision, all while maintaining the representativeness of the collected samples.

Microplastics, bisphenols, phthalates and pesticides in odontocete species in the Macaronesian Region (Eastern North Atlantic).

The gastrointestinal contents of twelve individuals from six odontocete species that stranded between 2018 and 2019 in the Macaronesian Region (Eastern North Atlantic) were examined for the presence of marine debris. In addition, concentrations of eleven organic persistent contaminants (nonylphenols, bisphenols, phthalates and pesticides) were analysed in muscle samples by liquid chromatography. No particles larger than 5 mm were found, except for two plastic labels that were found on the same dolphin. On the contrary, all animals contained microplastics of diverse sizes, most of them being fibres (98.06%, n = 708). The predominant detected pollutants were bisphenols (4-984 ng/g) and DEHP (102-1533 ng/g). Also, except for two individuals, all animals had pesticide levels in their tissues. This work has allowed the establishment of a protocol for the study of microplastic ingestion in cetaceans, and tests the potential of microRaman to improve the understanding of microplastic alteration processes.

Pump-underway ship intake: An unexploited opportunity for Marine Strategy Framework Directive (MSFD) microplastic monitoring needs on coastal and oceanic waters.

Broad scale sampling methods for microplastic monitoring in the open ocean waters remain a challenge in oceanography. A large number of samples is required to understand the distribution, abundance and fate of microplastic particles in the environment. Despite more than a decade of widespread study, there is currently no established time series of microplastic measurements and the research community is yet to establish a standardised set of methods that will allow data to be collected in a quick, affordable and interoperable way. We present a sampling technique involving the connection of a custom-built microplastic sampling device to the pump-underway ship intake system of a research vessel (RV) as an unexploited opportunity for oceanic monitoring needs concerning microplastic abundance and distribution. The method is cost effective, highly versatile and accurate, and is able to sample particles down to 50µm from opportunity platforms, thus contributing to an emerging area of study, and in particular helping to increase the monitoring reporting of data, and thereby serving as a valuable aid for the implementation of the Marine Strategy Framework Directive (MSFD). Sampling was performed during three consecutive oceanographic cruises in the subtropical NE Atlantic over a year, sampling subsurface waters (4 m depth) during navigation and while on coastal and oceanic stations. Microplastic particles were found in all stations and transects sampled. Fibres (64.42%) were predominant over fragments (35.58%), with the concentration values falling within the ranges of data reported for other areas of the Atlantic.