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1.
Artículo en Inglés | MEDLINE | ID: mdl-11250540

RESUMEN

The variability of sperm histones was compared in two species of sea urchin. Whole sperm specific histones (SpH), were isolated from Tetrapygus niger (Arbacoida) and Parechinus angulosus (Echinoida). Individual histones were purified by chromatography on BioGel P-60 followed by reverse high pressure liquid chromatography (HPLC). The heterogeneity of each major histone type from T. niger was established from their HPLC elution patterns and further confirmed by electrophoresis in polyacrylamide gels containing 6 mM Triton X-100 combined with a transverse urea gradient (0--8 M). In T. niger, as well as in P. angulosus, a single form of SpH1 and SpH2A were found. In contrast, SpH2B was found to be heterogeneous, but represented by one major form in both species. The relatedness between both sets of histones was determined by establishing their immunological cross-reactivity. In this context, polyclonal antibodies elicited against T. niger sperm histones were assayed against individual histones from P. angulosus. From the results obtained, it emerged that histone SpH2A was the more closely related protein between these two species, followed by histone SpH1. In contrast, histone SpH2B was found to be only moderately related. These results confirm that SpH2A did not co-evolve with SpH2B, as was predicted for most species.


Asunto(s)
Histonas/análisis , Erizos de Mar/química , Espermatozoides/química , Animales , Cromatografía Líquida de Alta Presión , Reacciones Cruzadas , Electroforesis en Gel de Poliacrilamida , Inmunoglobulina G/inmunología , Masculino , Conejos
2.
Biochem J ; 343 Pt 1: 95-8, 1999 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-10493916

RESUMEN

Fertilization in sea urchins is followed by the replacement of sperm-specific histones by cleavage-stage histone variants recruited from maternal stores. Such remodelling of zygote chromatin involves a cysteine proteinase that degrades the sperm-specific histones in a selective manner, leaving the maternal cleavage-stage histone variants intact. The mechanism that determines the selectivity of the sperm-histone-selective proteinase (SpH-proteinase) was analysed by focusing on the post-translational modification status of both sets of histones. It has previously been reported that only native cleavage-stage histones are poly(ADP-ribosylated), whereas the sperm-specific histones are not modified. To determine whether the poly(ADP-ribose) moiety afforded protection from degradation, the ADP-ribose polymers were removed from the cleavage-stage histones in vitro; these proteins were then assayed as potential substrates of the SpH-proteinase. Strikingly, the cleavage-stage histone variants were extensively degraded after the enzymic removal of their ADP-ribose moieties. In addition, the SpH cysteine proteinase was not inhibited by isolated poly(ADP-ribose) polymers. Consequently, only poly(ADP-ribosylated) cleavage-stage histone variants are protected from proteolysis. These results demonstrate a novel role for this type of post-translational modification, namely the protection of nuclear proteins against nuclear proteinases after fertilization.


Asunto(s)
Fertilización , Histonas/metabolismo , Poli Adenosina Difosfato Ribosa/metabolismo , Animales , Biopolímeros , Femenino , Erizos de Mar
3.
J Cell Biochem ; 76(2): 173-80, 1999 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-10618634

RESUMEN

At intermediate stages of male pronucleus formation, sperm-derived chromatin is composed of hybrid nucleoprotein particles formed by sperm H1 (SpH1), dimers of sperm H2A-H2B (SpH2A-SpH2B), and a subset of maternal cleavage stage (CS) histone variants. At this stage in vivo, the CS histone variants are poly(ADP-ribosylated), while SpH2B and SpH1 are phosphorylated. We have postulated previously that the final steps of sperm chromatin remodeling involve a cysteine-protease (SpH-protease) that degrades sperm histones in a specific manner, leaving the maternal CS histone variants unaffected. More recently we have reported that the protection of CS histones from degradation is determined by the poly(ADP-ribose) moiety of these proteins. Because of the selectivity displayed by the SpH-protease, the coexistence of a subset of SpH together with CS histone variants at intermediate stages of male pronucleus remodeling remains intriguing. Consequently, we have investigated the phosphorylation state of SpH1 and SpH2B in relation to the possible protection of these proteins from proteolytic degradation. Histones H1 and H2B were purified from sperm, phosphorylated in vitro using the recombinant alpha-subunit of casein kinase 2, and then used as substrates in the standard assay of the SpH-protease. The phosphorylated forms of SpH1 and SpH2B were found to remain unaltered, while the nonphosphorylated forms were degraded. On the basis of this result, we postulate a novel role for the phosphorylation of SpH1 and SpH2B that occurs in vivo after fertilization, namely to protect these histones against degradation at intermediate stages of male chromatin remodeling.


Asunto(s)
Fertilización/fisiología , Histonas/metabolismo , Espermatozoides/metabolismo , Secuencia de Aminoácidos , Animales , Cromatina/metabolismo , Endopeptidasas/metabolismo , Femenino , Histonas/genética , Histonas/aislamiento & purificación , Técnicas In Vitro , Masculino , Fosforilación , Erizos de Mar
4.
J Cell Biochem ; Suppl 32-33: 149-57, 1999.
Artículo en Inglés | MEDLINE | ID: mdl-10629114

RESUMEN

The role of proteolysis during fertilization has been investigated only to a very limited extent as compared with its role on the control of cell cycle progression. In this report, we discuss briefly the proteases involved in fertilization, their relevance in the egg-sperm interaction and in the chromatin remodeling that occurs before the reestablishment of the diploid condition of the zygote. We further emphasize how the post-translational modifications of target proteins modulate these proteolytic events. J. Cell. Biochem. Suppls. 32/33:149-157, 1999.


Asunto(s)
Endopeptidasas/metabolismo , Fertilización/fisiología , Procesamiento Proteico-Postraduccional , Animales , Cromatina/metabolismo , Cisteína Endopeptidasas/metabolismo , Femenino , Fertilización/genética , Histonas/metabolismo , Humanos , Masculino , Complejos Multienzimáticos/metabolismo , Óvulo/metabolismo , Complejo de la Endopetidasa Proteasomal , Espermatozoides/metabolismo , Ubiquitinas/metabolismo
5.
J Cell Biochem ; 63(4): 385-94, 1996 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-8978455

RESUMEN

To determine the changes in chromatin organization during male pronucleus remodeling, we have compared the composition of nucleoprotein particles (NP-ps) resulting from digestion with endogenous nuclease (ENase) and with micrococcal nuclease (MNase). Whole nuclei were isolated from sea urchin gametes and zygotes containing partially decondensed (15 min postinsemination, p.i.) or a fully decondensed (40 min p.i.) male pronucleus and digested with nucleases. The NP-ps generated were analyzed in agarose gels, and their histone composition was determined. Sperm core histones (SpH) and cleavage stage (CS) variants were identified by Western immunoblots revealed with specific antibodies. A single NP-ps was generated after digestion of sperm nucleus with MNase, which migrated in agarose gels between DNA fragments of 1.78-1.26 Kb. Sperm chromatin remained undigested after incubation in ENases activating buffer, indicating that these nuclei do not contain ENases. One type of NP-ps was obtained by digestion of unfertilized egg nuclei, either with ENase or MNase; the NP-ps was located in the region of the agarose gel corresponding to DNA fragments of 3.4-1.95 Kb [Imschenetzky et al. (1989): Exp Cell Res 182:436-444]. When whole nuclei from zygotes containing the female pronucleus and a partially remodeled male pronucleus were digested with ENase, a single NP-ps was generated, which migrated between DNA fragments of 2.5-1.9 Kb. This particle contained only CS histone variants. Alternatively, when these nuclei were digested with MNase, two NP-ps were generated; the slower migrating NP-ps (s) was located in the same position of the agarose gel as those resulting from ENase digestion and the faster migrating NP-ps (f) migrated between DNA fragments of 1.95-1.26 Kb. It was found that NP-ps (s) contained only CS histone variants, whereas NP-ps (f) were formed by a subset of SpH and by CS histone variants. When nuclei from zygotes containing a fully decondensed male pronucleus were digested either with ENase or MNase, a single type of NP-ps was observed, which migrated in the same position as NP-ps (s) in agarose gels. This particle contained only CS histone variants. On the basis of the histone compositions and on electrophoretic similarities, it was concluded that NP-ps (s) originated from the female pronucleus and that NP-ps (f) were generated from the partially remodeled male pronucleus. Consequently, our results indicate that at an intermediate stage of male pronucleus remodeling the chromatin is formed by NP-ps containing a subset of both SpH and of CS histone variants, whereas at final stages of male pronucleus decondensation chromatin organization is similar to that of the female pronucleus.


Asunto(s)
Núcleo Celular/química , Histonas/análisis , Nucleosomas/química , Espermatozoides/química , Cigoto/química , Animales , Western Blotting , Cromatina/metabolismo , ADN/aislamiento & purificación , Electroforesis en Gel de Agar , Endonucleasas/farmacología , Femenino , Variación Genética , Histonas/genética , Histonas/aislamiento & purificación , Masculino , Nucleasa Microcócica/farmacología , Nucleosomas/metabolismo , Oocitos/química , Erizos de Mar
6.
J Cell Biochem ; 61(1): 109-17, 1996 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-8726360

RESUMEN

Sea urchin CS histone variants are electrophoretically heterogeneous when analyzed in two dimensional polyacrylamide gels (2D-PAGE). Previous results suggested that this heterogeneity is due to the poly (ADP-ribosylation) of these proteins. Consequently, native CS histone variants were subjected to different treatments to remove the ADP-ribose moiety. The incubation in 1 M hydroxylamine was not effective in eliminating the polymers of ADP-ribose from CS variants, and the treatment with sodium hydroxide was deleterious to the proteins. In contrast, the ADP-ribose moiety was successfully removed from the CS variants by incubation with phosphodiesterase (PDE). To eliminate contamination of CS histone variants with PDE extract, the enzyme was covalently bound to Sepharose 4B prior to its utilization. Treatment of native CS histone variants with this immobilized phosphodiesterase removed around 85% of the total ADP-ribose moiety from these proteins. After S-PDE treatment the complex electrophoretic pattern of CS histone variants in 2-D PAGE decreases to five major fractions. From these results we conclude that the electrophoretic heterogeneity of native CS histone variants is mainly due to the extent to which five main CS histone variants are poly(ADP)-ribosylated).


Asunto(s)
Adenosina Difosfato Ribosa/metabolismo , Histonas/metabolismo , Animales , Western Blotting , Fase de Segmentación del Huevo , Compuestos de Dansilo/farmacología , Electroforesis en Gel de Poliacrilamida , Femenino , Colorantes Fluorescentes , Variación Genética , Glicosilación/efectos de los fármacos , Hidrazinas/farmacología , Hidrólisis , Hidroxilamina , Hidroxilaminas/farmacología , Masculino , Ácido Peryódico/farmacología , Fosfodiesterasa I , Hidrolasas Diéster Fosfóricas/farmacología , Poli Adenosina Difosfato Ribosa/análisis , Erizos de Mar , Hidróxido de Sodio/farmacología
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