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1.
Sci Rep ; 11(1): 6687, 2021 03 23.
Artículo en Inglés | MEDLINE | ID: mdl-33758295

RESUMEN

Intensive use of a few elite sires has increased the risk of the manifestation of deleterious recessive traits in cattle. Substantial genotyping data gathered using single-nucleotide polymorphism (SNP) arrays have identified the haplotypes with homozygous deficiency, which may compromise survival. We developed Japanese Black cattle haplotypes (JBHs) using SNP array data (4843 individuals) and identified deleterious recessive haplotypes using exome sequencing of 517 sires. We identified seven JBHs with homozygous deficiency. JBH_10 and JBH_17 were associated with the resuming of estrus after artificial insemination, indicating that these haplotypes carried deleterious mutations affecting embryonic survival. The exome data of 517 Japanese Black sires revealed that AC_000165.1:g.85341291C>G of IARS in JBH_8_2, AC_000174.1:g.74743512G>T of CDC45 in JBH_17, and a copy variation region (CNVR_27) of CLDN16 in JBH_1_1 and JBH_1_2 were the candidate mutations. A novel variant AC_000174.1:g.74743512G>T of CDC45 in JBH_17 was located in a splicing donor site at a distance of 5 bp, affecting pre-mRNA splicing. Mating between heterozygotes of JBH_17 indicated that homozygotes carrying the risk allele died around the blastocyst stage. Analysis of frequency of the CDC45 risk allele revealed that its carriers were widespread throughout the tested Japanese Black cattle population. Our approach can effectively manage the inheritance of recessive risk alleles in a breeding population.


Asunto(s)
Alelos , Genes Recesivos , Haplotipos , Mutación , Animales , Biomarcadores , Cruzamiento , Bovinos , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Variaciones en el Número de Copia de ADN , Desarrollo Embrionario , Homocigoto , Polimorfismo de Nucleótido Simple , Empalme del ARN , Secuenciación del Exoma
2.
Anim Sci J ; 91(1): e13415, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32671963

RESUMEN

Recessive missense mutation in the solute carrier family 12, member 1 (SLC12A1) gene (g.62382825G>A) is associated with hydrallantois, which is the accumulation of fluid in the allantoic cavity of a pregnant animal, and usually causes fetal death in Japanese Black cattle. However, the symptoms of a homozygote with this mutation that do not result in fetal death have not previously been tracked and evaluated. In the present study, we observed a homozygote with the SLC12A1 risk allele over a long-term period. The calf did not show any obvious clinical symptoms, although it did exhibit a slight growth retardation that accompanied mild calciuria. At 28 months of age, the homozygote showed renal dysfunction, which in turn resulted in hydronephrosis. The time course of the symptoms was consistent with the phenotype of Bartter syndrome in humans. Additionally, the risk heterozygous genotype did not any effects on carcass traits, which indicates that eliminating the risk allele would not have any unfavorable effects. Therefore, we emphasize that both the fetal- and late-stage symptoms associated with the SLC12A1 risk allele compromise animal welfare, and consequently may result in severe economic losses for individual farmers if the SLC12A1 risk allele is not eliminated from the population.


Asunto(s)
Síndrome de Bartter/genética , Síndrome de Bartter/veterinaria , Enfermedades de los Bovinos/genética , Bovinos/genética , Estudios de Asociación Genética/veterinaria , Homocigoto , Mutación Missense , Miembro 1 de la Familia de Transportadores de Soluto 12/genética , Alelos , Bienestar del Animal , Animales , Femenino , Hidronefrosis/genética , Hidronefrosis/veterinaria , Embarazo , Riesgo , Factores de Tiempo
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