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1.
J Vet Intern Med ; 30(6): 1882-1886, 2016 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-27731908

RESUMEN

BACKGROUND: Infection control is critical to providing high-quality patient care. Many veterinary teaching hospitals (VTHs) utilize footbaths or footmats at entrances and key control points throughout the facility to decrease trafficking of pathogenic microorganism on contaminated footwear. HYPOTHESIS/OBJECTIVES: To compare efficacy of 4 disinfectants used in footmats for decreasing bacterial contamination of footwear in a large animal hospital. ANIMALS: A single adult dairy cow was housed in a stall for 4 days to facilitate stall contamination with fecal material. METHODS: Overboots were experimentally contaminated with organic material in a standardized manner. Each boot was randomly assigned to 1 of 5 treatments (no treatment, or exposure to 1 of 4 disinfectants: an accelerated peroxygen [AHP], a peroxygen [VIRKON], a quaternary ammonium [QUAT], and a phenolic disinfectant [PHENOLIC]) by stepping on a soaked footmat and collecting samples from boot soles. Generalized linear modeling was used to analyze differences in bacterial counts. RESULTS: Reductions in colony-forming units (CFUs) on treated boots ranged from no detectable reduction to 0.45 log10 and varied by disinfectant. Percentage reductions in total bacterial counts generally were larger (albeit still modest) for AHP and QUAT disinfectants (range 37-45%) and smallest for the PHENOLIC (no detectable reduction). CONCLUSIONS AND CLINICAL IMPORTANCE: In general, use of disinfectant footmats was associated with significant reductions in viable bacteria on overboots-albeit with variable efficacy. Footmats may be useful adjuncts to cleaning and disinfection programs for decreasing trafficking of microorganisms throughout VTHs but should not be considered as a sole prevention method.


Asunto(s)
Bacterias/aislamiento & purificación , Recuento de Colonia Microbiana/veterinaria , Desinfectantes , Pisos y Cubiertas de Piso , Hospitales Veterinarios , Animales , Bovinos/microbiología , Heces/microbiología , Femenino , Humanos , Control de Infecciones/métodos , Peróxidos , Fenoles , Compuestos de Amonio Cuaternario , Zapatos
2.
J Vet Intern Med ; 30(4): 1197-203, 2016 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-27160025

RESUMEN

BACKGROUND: Histiocytic sarcoma (HS) is a rare but aggressive malignancy in humans that is poorly responsive to existing treatments. Although rare in most breeds of dogs, HS is common in Bernese mountain dogs (BMDs). OBJECTIVE: Determine risk factors associated with development of HS in BMD. ANIMALS: A total of 216 BMD were registered with the Berner-Garde Foundation. METHODS: An internet-based cross-sectional survey was used to collect information from owners of BMD diagnosed with HS and owners of disease-free littermates of dogs with HS. Mixed-effects logistic regression (MELR) and conditional logistic regression (CLR) were used in parallel to examine associations between potential risk factors and the occurrence of HS. RESULTS: When controlling for litter as a marker of relatedness, dogs diagnosed with orthopedic conditions were found to be more likely to develop HS (MELR, OR: 2.5, 95% CI: 1.5, 5.2; CLR, OR: 2.81, 95% CI: 1.1, 7.3), whereas dogs receiving prescription anti-inflammatory medications were found to be at considerably lower risk of developing HS (MELR, OR: 0.42, 95% CI: 0.2, 0.8; CLR, OR: 0.32, 95% CI: 0.1, 0.8). CONCLUSIONS AND CLINICAL IMPORTANCE: These results suggest inflammation may be a modifiable risk factor for the development of HS in BMD.


Asunto(s)
Enfermedades de los Perros/epidemiología , Sarcoma Histiocítico/veterinaria , Animales , Estudios Transversales , Enfermedades de los Perros/etiología , Enfermedades de los Perros/genética , Perros , Femenino , Sarcoma Histiocítico/epidemiología , Internet , Masculino , Linaje , Factores de Riesgo , Estados Unidos/epidemiología
3.
J Vet Intern Med ; 30(1): 215-22, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-26740174

RESUMEN

BACKGROUND: B-cell chronic lymphocytic leukemia (B-CLL) is the most common hematopoietic malignancy in humans in the developed world and the primary risk factor is genetic. Dogs also develop B-CLL, but there is no systematic description of the disease in dogs. Understanding the epidemiology of B-CLL in dogs may help practitioners recognize the disease and position the dog as a model for future genetic studies. OBJECTIVES: To describe B-CLL presentation in dogs, its clinicopathologic findings, and breed predisposition. ANIMALS: Four hundred and ninety-one dogs with B-CLL and 5,673 control dogs with suspicion of a lymphoproliferative disorder (LPD). METHODS: Retrospective cross-sectional study of dogs for which samples were submitted to the Colorado State University Clinical Immunology Laboratory for immunophenotyping between 2010 and 2014. To assess breed predilection, dogs with B-CLL were compared to those with suspicion of other LPDs using logistic regression. RESULTS: The median age was 11 years with no sex predilection. Half of the dogs presented with peripheral lymphadenopathy or splenomegaly and 26% had anemia. Eleven small-breed dogs had significantly increased odds of B-CLL. In addition, English Bulldogs had an increased risk and a unique presentation: these dogs were diagnosed at a median of 6 years and expressed lower class II MHC and CD25. CONCLUSIONS: B-cell chronic lymphocytic leukemia is overrepresented in small-breed dogs. Future genetic studies of these breeds may identify genetic risk factors. The unique presentation of English Bulldogs provides evidence of multiple forms of this disease. Additional studies are necessary to determine whether presenting signs are associated with survival.


Asunto(s)
Enfermedades de los Perros/genética , Predisposición Genética a la Enfermedad , Leucemia Linfocítica Crónica de Células B/veterinaria , Animales , Estudios Transversales , Enfermedades de los Perros/patología , Perros , Femenino , Leucemia Linfocítica Crónica de Células B/genética , Leucemia Linfocítica Crónica de Células B/patología , Masculino , Oportunidad Relativa , Estudios Retrospectivos
4.
J Vet Intern Med ; 29(2): 705-13, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25818224

RESUMEN

BACKGROUND: Mannheimia haemolytica is an important etiological agent in bovine respiratory disease. OBJECTIVES: Explore risk factors for recovery of susceptible and resistant M. haemolytica in feedlot cattle and explore associations with health outcomes. ANIMALS: Cattle (n = 5,498) from 4 feedlots sampled at arrival and later in feeding period. METHODS: Susceptibility of M. haemolytica isolates tested for 21 antimicrobials. Records of antimicrobial use and health events analyzed using multivariable regression. RESULTS: M. haemolytica recovered from 29% of cattle (1,596/5,498), 13.1% at arrival (95% CI, 12.3-14.1%), and 19.8% at second sampling (95% CI, 18.7-20.9%). Nearly half of study cattle received antimicrobial drugs (AMDs) parenterally, mostly as metaphylactic treatment at arrival. Individual parenteral AMD exposures were associated with decreased recovery of M. haemolytica (OR, 0.2; 95% CI, 0.02-1.2), whereas exposure in penmates was associated with increased recovery (OR, 1.5; 95% CI, 1.05-2.2). Most isolates were pan-susceptible (87.8%; 95% CI, 87.0-89.4%). AMD exposures were not associated with resistance to any single drug. Multiply-resistant isolates were rare (5.9%; 95% CI, 5.1-6.9%), but AMD exposures in pen mates were associated with increased odds of recovering multiply-resistant M. haemolytica (OR, 23.9; 95% CI, 8.4-68.3). Cattle positive for M. haemolytica on arrival were more likely to become ill within 10 days (OR, 1.7; 95% CI, 1.1-2.4). CONCLUSIONS AND CLINICAL IMPORTANCE: Resistance generally was rare in M. haemolytica. Antimicrobial drug exposures in penmates increased the risk of isolating susceptible and multiply-resistant M. haemolytica, a finding that could be explained by contagious spread.


Asunto(s)
Enfermedades de los Bovinos/microbiología , Mannheimia haemolytica/efectos de los fármacos , Infecciones por Pasteurellaceae/veterinaria , Animales , Antibacterianos/administración & dosificación , Antibacterianos/uso terapéutico , Bovinos , Enfermedades de los Bovinos/tratamiento farmacológico , Farmacorresistencia Bacteriana Múltiple , Análisis Multivariante , Infecciones por Pasteurellaceae/tratamiento farmacológico , Infecciones por Pasteurellaceae/microbiología , Factores de Riesgo , Estaciones del Año
5.
J Vet Intern Med ; 28(6): 1853-9, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25312330

RESUMEN

BACKGROUND: Salmonella enterica can significantly impact management of animal facilities. Comprehensive screening is essential for effective control in high-risk populations. Availability of reliable point-of-care diagnostic tests would facilitate these efforts. HYPOTHESIS/OBJECTIVES: Compare the ability of commercially available rapid diagnostic assays (2 lateral flow immunoassays [LFIs], DNA hybridization [DNAH], real-time PCR [qPCR]), and culture to detect common serotypes of S. enterica in feces. ANIMALS: n/a. METHODS: In an experimental study, 112 S. enterica isolates were randomly selected from the 10 most common serotypes recovered at a veterinary hospital. Archived isolates were amplified in broth and standardized inocula (100 colony forming units) were incubated with equine feces in tetrathionate broth (TET). Cultures were tested in a blinded fashion by using LFIs, DNAH, qPCR, and culture. RESULTS: The LFIs detected 84% and 67% of isolates, respectively, but reactivity varied among serotypes. Both reacted poorly with serotype Cerro (Group K) isolates, and 1 LFI did not react with any serotype Mbandaka (Group C1) or Montevideo (Group C1) isolates. DNAH detected 94% of isolates, whereas culture and qPCR most reliably detected all serotypes. False-positive results were obtained for 4 negative controls by using DNAH and 1 negative control by using qPCR, but LFIs and culture had no false-positive results. CONCLUSIONS AND CLINICAL IMPORTANCE: Culture, qPCR, and DNAH were effective in detecting most Salmonella isolates, but have limited application at point-of-care settings. LFIs are appealing as point-of-care tests because of low cost and ease of use, but limited detection of some serotypes needs to be evaluated with samples obtained from naturally infected animals.


Asunto(s)
Heces/microbiología , Enfermedades de los Caballos/microbiología , Salmonelosis Animal/microbiología , Salmonella enterica , Animales , Técnicas Bacteriológicas/veterinaria , Enfermedades de los Caballos/diagnóstico , Caballos , Inmunoensayo/veterinaria , Hibridación de Ácido Nucleico , Sistemas de Atención de Punto , Juego de Reactivos para Diagnóstico/veterinaria , Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria , Salmonelosis Animal/diagnóstico , Salmonella enterica/clasificación , Serotipificación/métodos , Serotipificación/veterinaria
6.
J Vet Intern Med ; 28(2): 538-46, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24495161

RESUMEN

BACKGROUND: Canine T-cell lymphoma (TCL) is conventionally considered an aggressive disease, but some forms are histologically and clinically indolent. CD4 TCL is reported to be the most common subtype of TCL. We assessed flow cytometric characteristics, histologic features when available, and clinical outcomes of CD4+ TCL to determine if flow cytometry can be used to subclassify this group of lymphomas. OBJECTIVE: To test the hypothesis that canine CD4+ T-cell lymphoma (TCL) is a homogeneous group of lymphomas with an aggressive clinical course. ANIMALS: Sixty-seven dogs diagnosed with CD4+ TCL by flow cytometry and treated at 1 of 3 oncology referral clinics. METHODS: Retrospective multivariable analysis of outcome in canine CD4+ TCL including patient characteristics, treatment, and flow cytometric features. RESULTS: The majority of CD4+ TCL were CD45+, expressed low class II MHC, and exhibited an aggressive clinical course independent of treatment regimen (median survival, 159 days). Histologically, CD4+ TCL were classified as lymphoblastic or peripheral T cell. Size of the neoplastic lymphocytes had a modest effect on both PFI and survival in this group. A small number of CD4+ TCL were CD45- and class II MHC high, and exhibited an apparently more indolent clinical course (median survival not yet reached). CONCLUSIONS AND CLINICAL IMPORTANCE: Although the majority of CD4+ TCL in dogs had uniform clinical and flow cytometric features and an aggressive clinical course, a subset had a unique immunophenotype that predicts significantly longer survival. This finding strengthens the utility of flow cytometry to aid in the stratification of canine lymphoma.


Asunto(s)
Recuento de Linfocito CD4/veterinaria , Enfermedades de los Perros/sangre , Linfoma de Células T/veterinaria , Animales , Antineoplásicos/uso terapéutico , Linfocitos T CD4-Positivos/patología , Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/tratamiento farmacológico , Enfermedades de los Perros/patología , Perros , Femenino , Citometría de Flujo/veterinaria , Linfoma de Células T/sangre , Linfoma de Células T/diagnóstico , Linfoma de Células T/tratamiento farmacológico , Linfoma de Células T/patología , Masculino , Estudios Retrospectivos , Resultado del Tratamiento
7.
J Vet Intern Med ; 27(6): 1535-42, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24112533

RESUMEN

BACKGROUND: Central nervous system blood vessel thrombosis is a part of the pathogenesis of equid herpesvirus-associated myeloencephalopathy (EHM). D-dimers (DD) are stable breakdown products of cross-linked fibrin, and increased DD-plasma concentrations could reflect the degree of systemic coagulation during EHV-1 infection. HYPOTHESIS: We hypothesized that blood DD concentrations will be increased during periods of EHV-1 fever and viremia, reflecting an activated coagulation cascade with fibrinolysis. ANIMALS: Twenty-eight equids were infected with EHV-1 in 3 experimental infection studies. Three (uninfected) horses were included in a separate study to evaluate methodology for DD concentration measurements. METHODS: Clinical data and quantitative viremia were evaluated, and DD concentrations were measured in blood samples on the day before the infection and during days 1-12 postchallenge. Uninfected horses were sampled every 3 hours for 48 hours. Logistic and linear regression was used to investigate the potential association between the fever and viremia with the presence or absence of DD concentrations in peripheral blood. RESULTS: DD concentrations were increased for 1-8 days in the majority of infected animals. Both viremia (odds ratio [OR] 6.3; 95% confidence interval [CI] 3.4-11.8; P = .0013) and fever (OR 4.9; CI 2.3-10.1; P = .001) were strongly associated with the likelihood of detecting DD in peripheral blood. CONCLUSIONS AND CLINICAL IMPORTANCE: EHV-1 viremia is associated with increases in DD concentration in horses and ponies. This indicates that EHV-1 viremia can lead to an activation of coagulation and fibrinolysis.


Asunto(s)
Productos de Degradación de Fibrina-Fibrinógeno/inmunología , Infecciones por Herpesviridae/veterinaria , Herpesvirus Équido 1/inmunología , Enfermedades de los Caballos/virología , Viremia/veterinaria , Animales , ADN Viral/química , ADN Viral/genética , Femenino , Productos de Degradación de Fibrina-Fibrinógeno/análisis , Infecciones por Herpesviridae/sangre , Infecciones por Herpesviridae/inmunología , Infecciones por Herpesviridae/virología , Enfermedades de los Caballos/inmunología , Caballos , Leucocitos Mononucleares , Masculino , Reacción en Cadena de la Polimerasa/veterinaria , Análisis de Regresión , Viremia/sangre , Viremia/inmunología , Viremia/virología
8.
J Vet Intern Med ; 27(6): 1392-9, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-24134779

RESUMEN

BACKGROUND: Expected rates of healthcare-associated infections (HCAI) have not been established in veterinary hospitals. Baseline rates are critically needed as benchmarks for quality animal care. OBJECTIVE: To estimate the occurrence of events related to HCAI identified using a standardized syndromic surveillance system in small animals in critical care cases at referral hospitals. ANIMALS: Weaned dogs and cats (n = 1,951) that were hospitalized in the critical care unit of referral teaching hospitals during a 12-week period. METHODS: Multicenter, prospective longitudinal study. A survey was completed for all enrolled animals to record basic demographics, information about procedures and treatments that animals received, and to document the occurrence of defined nosocomial syndromes. Data were analyzed to identify risk factors associated with the occurrence of these nosocomial syndromes. RESULTS: Controlling for hospital of admission, 16.3% of dogs (95% confidence intervals [CI], 14.3-18.5) and 12% of cats (95% CI, 9.3-15.5) were reported to have had ≥ 1 nosocomial syndrome occur during hospitalization. Risk factors found to have a positive association with the development of a nosocomial syndrome were longer hospital stays, placement of a urinary catheter, surgical procedures being performed, and the administration of antiulcer medications and antimicrobial drugs excluding those given perioperatively. CONCLUSIONS AND CLINICAL IMPORTANCE: Syndromic surveillance systems can be successfully standardized for use across multiple hospitals to effectively collect data pertinent to HCAI rates and risk factors for occurrence.


Asunto(s)
Enfermedades de los Gatos/epidemiología , Infección Hospitalaria/veterinaria , Enfermedades de los Perros/epidemiología , Monitoreo Epidemiológico/veterinaria , Animales , Gatos , Infección Hospitalaria/epidemiología , Perros , Femenino , Hospitales Veterinarios , Unidades de Cuidados Intensivos , Estudios Longitudinales , Masculino , Estudios Prospectivos , Factores de Riesgo
9.
Arch Virol ; 146(6): 1063-74, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11504416

RESUMEN

We reported previously that equine-2 influenza A virus (H3N8) had evolved into two genetically and antigenically distinct "Eurasian" and "American" lineages. Phylogenetic analysis, using the HA1 gene of more recent American isolates, indicated a further divergence of these viruses into three evolution lineages: A South American lineage, a Kentucky lineage, and a Florida lineage. These multiple evolution pathways were not due to geographic barriers, as viruses from different lineages co-circulated. For the Kentucky lineage, the evolution rate was estimated to be 0.89 amino acid substitutions per year, which agreed with the previously estimated rate of 0.8. For the South American lineage, the evolution rate was estimated to be only 0.27 amino acid substitutions per year. This low evolution rate was probably due to a unique alternating Ser138 to Ala138 substitutions at antigenic site A. For the Kentucky lineage, there was a preference for sequential nonsynonymous substitutions at antigenic site B, which was also a "hot spot" for amino acid substitutions. Convalescent sera had minimal cross-reactivity to viruses of different lineages, indicating antigenic distinctions among these viruses. In contrast to human H3N2 viruses, our results suggested that the evolution of equine-2 influenza virus resembled the multiple evolution pathways of influenza B virus.


Asunto(s)
Evolución Molecular , Subtipo H3N8 del Virus de la Influenza A , Virus de la Influenza A/genética , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Animales , Antígenos Virales/genética , Florida , Genes Virales , Hemaglutininas Virales/genética , Caballos , Humanos , Virus de la Influenza A/clasificación , Virus de la Influenza A/inmunología , Virus de la Influenza A/aislamiento & purificación , Kentucky , Datos de Secuencia Molecular , América del Sur , Factores de Tiempo , Proteínas Virales/genética , Proteínas Virales/inmunología
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