[Use of cell cultures in the local treatment of burn wounds]. / Ispol'zovanie kletochnykh kul'tur pri mestnom lechenii ozhogovykh ran.

The article deals with the results of using cultivated allofibroblasts in topical treatment of burn wounds. Allofibroblasts obtained from the derma of burnt individuals were transplanted to the burn wounds of 28 patients. In 12 among 15 patients (80%) with IIIa-IIIb degree burns transplantation induced epithelialization of the burns in 12.1 +/- 2.4 days. Thirteen patients had deep burns, only in 3 of them with a circumscribed burn area transplantation proved effective. Complications of transplantation were encountered in 5 patients--rejection of the transplant in 2 and its suppuration in 3 patients. Allofibroblast transplantation may be applied as an independent method of treatment in IIIa, b degree burns.

[Treatment of chronic non-healing donor sites: transplantation of cultured human fibroblasts]. / Lechenie dlitelno ne zazhivaiushchikh donorskikh uchastkov: transplan tatsiia kultivirovannykh allofibroblastov cheloveka.

The authors discuss the first results of the use of cultivated human allofibroblasts in the treatment of persisting wounds of the donor areas in 13 patients with deep burns and cicatricial deformities caused by the burns. Transplantation of cell cultures led to complete epithelialization of the wounds in 6-10 days. Complication, suppuration of the grafted material, was recorded in one case. Reaction of graft rejection was not encountered. The authors believe that the effect of the transplantation is due to stimulation of the epithelialization processes by the cultivated fibroblasts.

[Cultured human skin cells in the treatment of burns]. / Lechenie ozhogov s ispolzovaniem kultivirovannykh kletok kozhi cheloveka.

The work discusses the results of experience of long-year's standing of the staff of the Vishnevsky Institute of Surgery in the creation of a biological bandage with the use of cultivated human skin cells. A graft of cultivated human allofibroblasts was used for closure of burn wounds for the first time. Its efficacy was studied in 86 cases of burns. The use of cultivated allofibroblasts is indicated in bordering III ab degree burns and sluggishly healing donor areas formed after autodermoplasty. Study of the efficacy of cultivated allofibroblasts in the treatment of deep burns in combination with dermoplasty with a 1:6 perforated skin autograft are continued. The low cost and technical simplicity of the method allow cultivated fibroblast grafts to be recommended for wide clinical practice.

[The modern method of treating burn wounds]. / Sovremennaia metodika lecheniia ozhogovykh ran.

An autoplasty method for treatment of burns was worked out by the authors. In lab conditions a monolayer of epithelium is grown out from a piece of owner skin. Its size is 10 times (and more) as big as the initial material. The article deals with the results of treatment of 12 patients who had the transplantation of monolayer epithelium. Indications and contraindications are enclosed. The article concludes by the appreciation of data which were obtained during this work.

[Allotransplantation of cultured fibroblasts on non-healing wounds after autodermatoplasty]. / Allotransplantatsiia kul'tivirovannykh fibroblastov na nezazhivaiushchie rany posle autodermoplastiki.

An allograft from man cultured fibroblasts has been received. Results of its transplantation on long not-healing donor wounds in 13 patients after autodermatoplastic operations have been studied. Expressed stimulating influence of cultured fibroblasts on donor wound epithelialization processes has been stated. In spite of allograft applications there has been no tearing away reactions. It has been connected with elimination of superficial cell antigens during cultivation. Microbe contamination of more than 10(2) is the contraindication for grafting.

[Use of cultured fibroblasts in the treatment of burn wounds]. / Ispol'zovanie kul'tivirovannykh fibroblastov pri lechenii ozhogovykh ran.

This paper deals with the possibility of closing of burn surfaces of skin wounds with cultivation of patients' fibroblasts. Transplantation of cultured fibroblasts was performed in 11 patients with 30-75% of a body surface burned. The results obtained indicate a prospect of this method of burn treatment.

[Cultivation of epidermocytes of experimental animals and man]. / Kul'tivirovanie épidermotsitov éksperimental'nykh zhivotnykh i cheloveka.

Experimental animal and human biopsy material has been evaluated for the possibility of culturing skin epidermocytes. Insulin and hydrocortisone were used as biostimulants of adequate cell proliferation. Morphological studies revealed that the culture obtained exists as a monolayer of epidermocytes free of other skin elements.

[Acute cholecystitis in severely burned patients]. / Ostryi kholetsistit u tiazheloobozhzhennykh.

Acute acalculous cholecystitis in burned patients develops due to general disturbances of microcirculation of polyetiological origin. Early laparotomy can facilitate diagnosis of acute acalculous cholecystitis in burned patients. Rapidly developing destruction of the gallbladder walls requires an active surgical tactics.

[Electron radioautographic study of a human infected wound]. / Elektronno-radioavtograficheskoe izuchenie infitsirovannoi rany cheloveka.

Biopsies from burn (IIIa-IV degree, 20-55% of body surface) and large traumatic wounds of 18 patients were studied. The use of electron-microscopic radioautography made it possible to determine the viability and functional activity of bacteria and host cells in various wound areas. In the necrotic area, apart from a number of viable bacterial cells (intensely labelled with 3H-uridine), unlabelled bacterial cells with various structural alterations were found. Bacteria were found less frequently in the demarcation area; they were detected occasionally in the granulation tissue and only in macrophages. Phagosomes with viable or dead bacteria were observed in few neutrophils of the demarcation area. Here, the majority of neutrophils did not contain bacteria, but degranulation and many phagosomes with tissue detritus were observed. Correlation between the rate of RNA synthesis and functional activity of neutrophils was observed. In the blood neutrophils the RNA synthesis was not detected at all. In the blood neutrophils which were cultivated in vitro with staphylococci and in the wound neutrophils phagocytizing bacteria, the RNA synthesis became evident but slow. The authors established that a sharp increase of the RNA synthesis rate occurs in the neutrophils phagocytizing the dead tissues.