RESUMEN
We study the effect of soft gluon emission in the hadroproduction of squark-antisquark and gluino-gluino pairs at the next-to-leading logarithmic (NLL) accuracy within the framework of the minimal supersymmetric model. The one-loop soft anomalous dimension matrices controlling the color evolution of the underlying hard-scattering processes are calculated. We present the resummed total cross sections and show numerical results for proton-proton collisions at 14 TeV. For the gluino-pair production, the theoretical uncertainty due to scale variation is reduced to the few-percent level.
RESUMEN
BACKGROUND: Administration of a chemically defined, liquid, elemental diet (CDD) results in intestinal microbial translocation, but the immunologic consequences of this process are unclear. This study evaluated the effects of CDD feeding and interleukin-4 (IL-4) administration on mesenteric lymphocyte, peritoneal macrophage (PMO), and hepatic Kupffer cell (KC) functions. METHODS: BALB/C mice (n = 60) were randomized to receive a paired feeding of regular diet (RD) or a CDD for 14 days. Mesenteric lymph nodes (MLN) and cecum were cultured for bacteria. Mixed lymphocyte response and cytotoxic T-lymphocyte function of MLN lymphocytes were assayed. PMO and KC were harvested to measure tumor necrosis factor production, macrophage binding of fluorescent-labeled lipopolysaccharide, and Candida albicans phagocytosis (CAP) and killing (CAK); KC-hepatocyte interaction was assessed by hepatocyte protein synthesis. In a second study 75 BALB/c mice received RD, CDD, and CDD+IL-4 (30,000 units/mouse intraperitoneally). MLN lymphocyte and PMO functions were measured and intestinal immunoglobulin A levels were determined. RESULTS: Oral feeding of a CDD resulted in significant impairment of mesenteric lymphocyte mixed lymphocyte response and cytotoxic T-lymphocyte functions and decreased PMO tumor necrosis factor production, fluorescent-labeled lipopolysaccharide binding, CAP, and CAK. KC function was preserved in CDD-fed mice. Administration of IL-4 significantly reduced the incidence of bacteria positive MLN and increased PMO superoxide production, CAP, CAK, and MLN lymphocyte mitogenesis. CONCLUSIONS: Use of IL-4 may be beneficial in situations where intestinal microbial translocation contributes to sepsis.
Asunto(s)
Fenómenos Fisiológicos Bacterianos , Alimentos Formulados , Interleucina-4/inmunología , Intestinos/microbiología , Animales , Células Cultivadas , Interleucina-4/farmacología , Macrófagos del Hígado/efectos de los fármacos , Macrófagos del Hígado/fisiología , Prueba de Cultivo Mixto de Linfocitos , Linfocitos/efectos de los fármacos , Linfocitos/fisiología , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/fisiología , Mesenterio/citología , Ratones , Ratones Endogámicos BALB C , Distribución Aleatoria , Linfocitos T Citotóxicos/fisiologíaAsunto(s)
Neoplasias/terapia , Nutrición Parenteral Total , Anorexia/inducido químicamente , Antineoplásicos/efectos adversos , Humanos , Neoplasias/tratamiento farmacológico , Neoplasias/metabolismo , Trastornos Nutricionales/etiología , Trastornos Nutricionales/terapia , Ensayos Clínicos Controlados Aleatorios como AsuntoRESUMEN
CHO-K1D cells electroporated in buffers containing [32P]NAD incorporated the label in a voltage-dependent manner. Electroporation with 650 V/cm at 1460 microF in Ham's F12 medium supplemented with 10 mM Hepes, pH 7.1, resulted in a greater than 20-fold increase in [32P]NAD uptake, while decreasing relative cellular survival by only 6%. Exposure of cells to gamma irradiation (20 Gy) prior to electroporation increased the steady-state level of poly(ADP-ribosylated) nuclear proteins two- to four-fold over that of unirradiated control cells. These data indicate that electrotransfer of [32P]NAD is a simple and rapid means of labeling the cellular NAD pool and should prove useful in the analysis of the relationship between poly(ADP-ribosylation) of nuclear proteins and DNA repair.
Asunto(s)
Adenosina Difosfato/análisis , NAD , Proteínas Nucleares/análisis , Ribosa/análisis , Adenosina Difosfato/metabolismo , Adenosina Difosfato/efectos de la radiación , Animales , Tampones (Química) , Células Cultivadas , Cricetinae , Electricidad , Electroforesis en Gel de Poliacrilamida , Rayos gamma , NAD/metabolismo , Proteínas Nucleares/metabolismo , Proteínas Nucleares/efectos de la radiación , Ribosa/metabolismo , Ribosa/efectos de la radiaciónRESUMEN
A method to detect single-stranded DNA damage from individual cells has been developed using a monoclonal anti-thymidine antibody (MoAb20B7). Initially, HL-60 cells were incubated with daunomycin at different concentrations, and processed by MoAb20B7. While 73.5% of the cells incubated with 5 micrograms/ml of daunomycin for 24 h reacted positively with MoAb20B7, 83.5% cells at 10 micrograms/ml daunomycin dose were positive. Next, this method was combined with unscheduled DNA synthesis to simultaneously measure repair and damage from individual cells. Finally, patients with acute myeloid leukemias were studied before and 24 h after therapy with a daunomycin containing regimen. In vivo damage could be determined in a prompt fashion.
Asunto(s)
Anticuerpos Monoclonales , Daño del ADN , ADN de Cadena Simple , Timidina/inmunología , Animales , Reparación del ADN , ADN de Cadena Simple/efectos de los fármacos , Daunorrubicina/farmacología , Humanos , Leucemia Mieloide Aguda/tratamiento farmacológico , Leucemia Mieloide Aguda/genética , Ratones , Ratones Endogámicos BALB CRESUMEN
The ability of a new monoclonal antibody against thymidine (MoAb 20B7) to detect chemotherapy induced single stranded DNA damage is described. HL-60 cells were used for these experiments. Damage to DNA was caused by incubation of cells with alkylating agents. Portions of DNA which is damaged are cleaved by cellular endonucleases, thus exposing thymidine on the opposite DNA strand. Processing of these samples by MoAb 20B7 showed that such damaged segments could be detected consistently. Furthermore, this method was combined with autoradiographic detection of unscheduled DNA synthesis thereby allowing for assessment of DNA repair simultaneously from the same cell.