RESUMEN
Recent studies have demonstrated that resveratrol can reduce blood sugar, improve insulin resistance, regulate abnormalities in lipid metabolism, and lower the secretion and expression of inflammatory factors. The present study investigated the antiinflammatory effects of resveratrol in animal models of acute pharyngitis, and its possible mechanisms. Commercial ELISA kits were used to measure tumor necrosis factorα, interleukin (IL)6, macrophage inflammatory protein2, cyclooxygenase2 levels and caspase3/9 activity. Tolllike receptor (TLR)4, myeloid differentiation primary response protein MyD88, phosphorylated (p)nuclear factor (NF)κB and pIκB were analyzed using western blotting. In a rabbit model of acute pharyngitis, it was demonstrated that resveratrol inhibited tumor necrosis factorα and interleukin6 serum levels, macrophage inflammatory protein2 and cyclooxygenase2 activity levels, reactive oxygen species production and caspase3/9 activity. Resveratrol suppressed NACHT, LRR and PYD domainscontaining protein 3 and caspase1 protein expression, and reduced IL1ß and IL18 protein expression in animal models of acute pharyngitis. Additionally, resveratrol suppressed TLR4 and myeloid differentiation primary response protein 88 protein expression, and reduced pNFκB and increased pIκB protein expression in animal models of acute pharyngitis. In conclusion, these findings indicated that the antiinflammatory activity of resveratrol prevents acute pharyngitisinduced inflammation by inhibiting NFκB in animal models. Therefore, these data suggested an important clinical application of resveratrol in preventing acute pharyngitis.