RESUMEN
Bupleurum plantagineum Desf. (Apiaceae) is a plant endemic to North Africa, including Algeria. It is used as natural medicine, due to its anti-inflammatory, antioxidant and hepatoprotective properties. In this work, we studied the chemical composition of the essential oil (EO) obtained by hydro-distillation from B. plantagineum leaves collected from the national park of Gouraya (Bejaïa), Algeria. Thirty components were identified in the EO by Gas chromatography-Mass Spectrometry(GC-MS) (relative abundance: 96.7%). Monoterpene hydrocarbons (56.8%) were the predominant, followed by oxygenated monoterpenes (36.7%). The main components were cis-chrysanthenyl acetate (33.5%), α-pinene (18.4%), myrcene (16.5%) and (E)-anethole (4.9%). This volatile profile differs from those reported for other Bupleurum spp. and for B. plantagineum from Algeria, suggesting the identification of a new chemotype. Overall, our results represent a contribution to the characterisation of natural products from Algeria and the preservation of its natural biodiversity. The same results will also represent a starting point for further studies on B. plantagineum.
RESUMEN
A bacteriocin-producing strain (9,000 AU/ml) was isolated from the rhizosphere of Algerian healthy plants Ononis angustissima Lam. and identified as Bacillus clausii strain GM17. The bacteriocin, called Bac-GM17, was purified from the culture supernatant after heat treatment, ammonium sulfate precipitation, Sephadex G-50 chromatography and Mono Q fast-performance liquid chromatography (FPLC). Based on matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis, the purified Bac-GM17 is a monomer protein with a molecular mass of 5,158.11 Da. The N-terminal sequencing allowed for the straightforward identification of its first 20 residues, which were of pure bacteriocin. It also revealed that this bacteriocin contained a unique sequence, namely DWTCSKWSCLVCDDCSVELT, which suggests the identification of a novel compound. Bac-GM17 was extremely heat stable (20 min at 120 °C) and was stable within the pH range (3-9). It was found to be resistant to the proteolytic action of trypsin, pepsin, papain, pronase E, and proteinase K. It was also noted to display a bactericidal mode of action against Agrobacterium tumefaciens C58 and a fungistatic mode of action against Candida tropicalis R2 CIP203.
