RESUMEN
Chemical investigation of Cordia myxa L. (Boraginaceae) resulted in the isolation of the following ten known compounds: 1-naphthaleneacetic-5-carboxy-1,2,3,4,4a,7,8,8a-octahydro-1,2,4a-trimethyl-[1S-(1α,2ß, 4a,8aα)]-acid (1), hexacosanoate-1-glyceryl (2), 3ß-urs-12,20(30)-diene-27,28-dioic acid (3), 3ß-D-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid (4), stigmasterol (5), stigmasterol-3-O-ß-D-glucopyranoside (6), oleanolic-acid (7), 3-O-acetyl-oleanolic acid (8), betulin (9) and spinasterol-3ß-O-D-glucopyranoside (10). The isolated compounds were characterised by using spectroscopic methods, 1D and 2D NMR, mass spectroscopy (ESI-MS) and by comparison with the literature data. To the best of our knowledge, compounds 1, 3, 4, 8 and 10 were isolated for the first time from the Cordia genus. This result improves the chemotaxonomy knowledge of the Cordia genus. The antibacterial activities were performed by the Muller-Hinton agar diffusion method. The antibacterial activities were studied on Salmonella typhi, Staphylococcus aureus, Vibrio cholerae, Pseudomonas aeruginosa and Escherichia coli ATCC 25922. Compounds 8 and 9, at 20.0 mg/mL resulted to be effective antimicrobial against E. coli, V. cholerae and P. aeruginosa.
RESUMEN
A new triterpenoid saponin, Ternifoliasaponin (1), together with four known compounds (2-5) chikusetsusaponin IVa (2), chikusetsusaponin IVa methyl ester (3), bonushenricoside B (4) and Dianoside C (5) were isolated from roots of Gardenia ternifolia Schumach. & Thonn (Rubiaceae). The structures of isolated compounds were elucidated on the basis of spectroscopic analysis and chemical methods. The antibacterial activities of compounds (3), and (4) were performed by the Muller-Hinton agar diffusion method. The antimicrobial activities of the compounds were studied on Salmonella typhi (Enterobacteriaceae), Staphylococcus aureus and Pseudomonas aeruginosa microorganisms. Compound (3) at 25 mg/mL, showed moderately sensitive effect (8.0 Ë DIZ Ë14.0 mm) on S. typhi, S. aureus and P. aeruginosa. Compound (4) at 25 mg/mL and compound (3) at 12.5 mg/mL exhibited moderately sensitive effect on S. typhi and S. aureus. Compound (4) inhibited moderately sensitive the S. typhi and P. aeruginosa colonies at 12.5 mg/mL.