Violence on social networks related to the body image of young women and its repercussions on health.

INTRODUCTION: Social networking sites perpetuate gender stereotypes, inequalities, and gender violence, generating adverse health effects. METHODS: This study aimed to analyze the relationship between Instagram, alcohol consumption and effects on health, through interviews and discussion groups involving adolescents (high school or university students and workers), during the period from the third to the sixth wave of the COVID-19 pandemic. RESULTS: A total of 118 individuals participated in the study. Three coding clusters were identified: (1) Inequality in the representation of men and women on SNSs; (2) Violence towards women's bodies and sexual violence on SNSs; and (3) Health problems derived from violence against women's body image on SNSs. The main focus of our analysis was the exhibition of the female body, in which eroticization of the body prevails and gender roles are perpetuated. Violence is generated towards women in the form of misogynistic attacks for daring to challenge conventional stereotypes. Many interviewees coincide that the main health effects include low self-esteem, anxiety and eating disorders. CONCLUSIONS: The institutional response to the abuses and violence committed on SNSs against women is weak, and public policies are needed to protect women in these environments where they are especially vulnerable.

Cross-Cultural Adaptation and Validation of the Portuguese Version of the Multidimensional Scale of Dating Violence 2.0 in Young University Students.

BACKGROUND: Dating violence has become a problem of social relevance with short- and long-term health consequences. Nurses are in a privileged position to detect and address this problem in health facilities and as school nurses in schools, providing health education and detecting this violence correctly. AIM: The aim of this study was to evaluate the cross-cultural validation of the Portuguese version of the Multidimensional Scale of Dating Violence-Short (MSDV 2.0). METHODS: A validation investigation was carried out in two phases: (1) cross-cultural adaptation of the items and content validation of the Portuguese version of MSDV 2.0 and (2) psychometric validation. RESULTS: Phase (1): The items of the original version include a cross-cultural translation from Spanish to Portuguese and analysed by a group of experts in gender violence and by the authors of the original scale, then a back translation was made and again reviewed by the experts. Young university students also participated for face validity, and a pilot test was carried out. Phase (2): Confirmatory factor analysis was performed using the robust maximum-likelihood estimation method, which confirmed the five-dimensional structure, obtaining good fit rates (chi-square significance (χ2) = 187.860 (p < 0.0001); root mean square error of approximation (RMSEA) = 0.049; comparative fit index (CFI) = 0.937; Tucker-Lewis index (TLI) = 0.923). Reliability analysis indicated adequate internal consistency (Cronbach's alpha (α) = 0.88 to 0.70). Finally, scores of the Portuguese versions MSDV 2.0 were correlated, as expected, positively with the Depression, Anxiety, and Stress Scale (DASS-21) (r = 0.36 to 0.16) and negatively with the Medical Outcomes Study Questionnaire Short Form 36, Health Survey (SF-36) (r = -0.30 to -0.14). CONCLUSIONS: To date, it is the only instrument that measures dating violence in a multidimensional way validated in the Portuguese university context.

"The COVID 19 pandemic worsened my living and working conditions". A qualitative research study of female Brazilian immigrants in Oporto.

Immigrant women face a double vulnerability, being a woman and an immigrant, a situation that hinders their entry into the labor market and worsens their working conditions and occupational health with respect to the native population. The objective of these women is to seek employment or educational opportunities and improving their own lives and those of their families. All this is affected by the axes of gender, ethnicity, migration and socioeconomic level, as well as being confronted with the patriarchal structure of exercise of power and domination. These situations of inequality have aggravated due to the COVID-19 pandemic, worsening working conditions and their occupational health. This study aims to explore the working conditions of the female Brazilian immigrant population living in Porto (Portugal) and how these conditions may affect their health. Qualitative research through semi-structured interviews conducted during the second wave of the COVID-19 pandemic was used. The results show of them had work overload, manifesting anxiety and stress. Physical affectations related to poor work ergonomics and the lack of occupational health examinations in working immigrant women is highlighted. The importance of strengthening migration policies related to occupational health is highlighted. In pandemic situations, the vulnerability of these women increases, worsening their overall health.

Acculturation, Health Behaviors, and Social Relations among Chinese Immigrants Living in Spain.

This study aims to identify acculturation experiences about social relations and health behaviors of first-generation Chinese immigrants in the South of Spain, including food patterns, physical exercise, and tobacco and alcohol use. A phenomenological qualitative study was conducted using semi-structured interviews, informal conversations, and field notes. All data were analyzed under the Berry's Model of Acculturation. A total of 133 Chinese immigrants were included. Our findings show that separation was the dominant acculturation strategy, followed by integration and assimilation, while marginalization was not present in this immigrant population. Most of the immigrant population maintains a link to the customs of their home country, favoring the process of identity and collective self-esteem. These results can help health managers and the government to further understand Chinese immigrants in Europe and to establish appropriate health interventions to this group.

Adherence to a Supplemented Mediterranean Diet Drives Changes in the Gut Microbiota of HIV-1-Infected Individuals.

OBJECTIVE: The health effects of a supplemented Mediterranean diet (SMD) with extra-virgin olive oil (EVOO) and nuts are well documented in non-HIV-infected individuals. We hypothesised that the benefits of an SMD could be mediated by changes in the gut microbiota, even in those with an altered intestinal microbiota such as people living with HIV. DESIGN: Individuals living with HIV (n = 102) were randomised to receive an SMD with 50 g/day of EVOO and 30 g/day of walnuts (SMD group) or continue with their regular diet (control group) for 12 weeks. METHODS: Adherence to the Mediterranean diet was assessed using the validated 14-item MD-Adherence-Screener (MEDAS) from the PREDIMED study. A sub-study classifying the participants according to their MEDAS scores was performed. RESULTS: The lipid profile was improved in the SMD group vs. that in the control group (delta-total cholesterol and delta-B-lipoprotein). The immune activation (CD4+HLADR+CD38+ and CD8+HLADR+CD38+ cells) and IFN-γ-producing T-cells significantly decreased at week 12 compared to the baseline in the SMD group but not in the control group. The gut microbiota in those from the high-adherence group presented significantly high diversity and richness at the end of the intervention. Succinivibrio and Bifidobacterium abundances were influenced by the adherence to the MD and significantly correlated with Treg cells. CONCLUSION: The Mediterranean diet improved metabolic parameters, immune activation, Treg function, and the gut microbiota composition in HIV-1-infected individuals. Further, Mediterranean diet increased the Bifidobacterium abundances after the intervention, and it was associated to a beneficial profile.

Food Patterns among Chinese Immigrants Living in the South of Spain.

This article aims to explore the food patterns of Chinese immigrants living in Spain and to understand the factors associated with these behaviors. This qualitative ethnographic study included 133 Chinese immigrants; using interviews with scripts were based on the National Health Survey of Spain. Three categories were defined: "Differences between Chinese food and Western food"; "Products and dishes consumed by Chinese immigrants" and "Modification of eating habits". Participants found a remarkable difference between eastern (i.e., vegetables and rice) and western (i.e., dairy, high-fat, bread) foods. They also experienced a change in their eating habits, mostly related to acculturation and lack of time. Chinese immigrants have different eating patterns as compared to the native population in Spain and this information could help in future healthcare strategies. Knowing the food culture could infer aspects, such as insertion or integration, and promoting health and well-being.

Importance of Mediation against Addictive, Affective-Emotional, and Sexual Behavior in Adolescents. Educational System versus Associations.

This article analyzes health mediation among equals as an educational strategy against risk behaviors in young people from both the educational and associative systems in Seville (Spain), based on qualitative research, with the aim of assessing and comparing its impact in those areas. To this end, interviews with 49 professionals and 427 adolescents were conducted in discussion groups. Results acknowledge mediation as individual or group intervention accepted by young people as advice and information on health issues and conflict resolution, but also as a method for data collection in order to obtain a community health diagnosis. The educational system implements this strategy, but in associations it seems to work better, particularly in the psycho-emotional and sexual spheres. Unfortunately, intervention programs are usually discontinuous due to lack of resources and territorial variability. And this is why mediators' support is highly valued by the target users, with preference for a male figure in the case of boys, and larger predisposition towards a female mediator in girls, except in cases where this agent has a special social relevance.

HIV-DNA content in pTfh cells is associated with residual viremia in elite controllers.

OBJECTIVE: The source of residual HIV viremia is highly debated and its potential relationship with the HIV reservoir has not been clarified. Herein, we analysed the cell-associated HIV-DNA content in two important cell compartments of the HIV reservoir, resting CD4+ T memory (Trm) and peripheral T follicular helper (pTfh) cells, and the association with the residual HIV viremia in individuals with spontaneous HIV replication control (elite controllers, EC group) and in individuals with antiretroviral therapy (ART)-mediated HIV replication control (cART group). DESIGN: A cross-sectional study. METHODS: Seventeen chronically HIV-infected patients with suppressed HIV replication were included. Cell-associated HIV-DNA was measured by ultrasensitive digital-droplet-PCR in purified Trm and pTfh cells. Residual HIV plasma viremia was quantified using a single-copy assay with a sensitivity of 0.3 HIV-RNA copies/ml. RESULTS: A significant and positive correlation was demonstrated between HIV-DNA levels in pTfh cells and residual plasma viral load (rpVL) (rho = 0.928, P = 0.008) in HIV-positive elite controllers, but not in HIV-positive treated patients, despite the lower levels of cell-associated HIV-DNA found in elite controllers compared with cART patients in pTfh cells [176 (77-882) vs. 608 (361-860) copies/million cells, respectively; P = 0.05]. CONCLUSION: This association suggests that pTfh cells could have an important contribution to persistent viremia in elite controllers. This could be the consequence of a more limited control of HIV replication in elite controllers with higher transcriptional activity of HIV in pTfh cells of elite controllers than that in cART patients.

The Occupational Health of Female Immigrant Caregivers: A Qualitative Approach.

In general, immigrants suffer poor working conditions. This is particularly true in the case of women, who constitute 48% of international migrants, and these poor conditions are closely linked to the sectors they mainly occupy, such as domestic and care-giving services. The aim of the present study was to investigate the working conditions of the female immigrant population living in southern Spain and how these conditions may affect their health. A qualitative study using semi-structured interviews and discussion groups was conducted over one year in 2019, with 61 immigrant women recruited. The sectors occupied by immigrant women were caregiving for dependent people and domestic services. Most of the female immigrants interviewed were working (63.94%), although the majority were employed in an irregular situation, with a very long working day. Among the main risks identified were biological risks, physical attacks, falls, wounds and musculoskeletal complaints related to handling patients and carrying out household chores. Most of them had not taken an occupational health test and did not report accidents occurring in the workplace for fear of losing their jobs. The main health problems were related to physical and mental health (such as musculoskeletal diseases and stress). These findings highlight the importance of making a major change in our perspective regarding the social value of including immigrant women in the labour market and the different aspects related to their health.

Consumption of Substances in Nightlife Settings: A Qualitative Approach in Young Andalusians (Spain).

Adolescence and youth are stages of exploration and experimentation, when the consumption of psychoactive substances for recreational or experimental purposes often begins. The general objective of this study was to explore youth consumption habits in nightlife settings and associated factors in Andalusia (Spain). To this end, we took into account young people's perceptions about patterns of drug polyconsumption in nightlife settings and the perceptions and actions of health and teaching professionals towards this issue. We carried out a qualitative methodology with 24 in-depth interviews and 3 discussion groups with Andalusian girls and boys aged between 16 and 22 (n = 45) and 13 in-depth interviews with social agents (health and teaching professionals). We performed narrative discourse analysis and triangulation of identified categories and measured the units of analysis. The results show information relating to gender, age of initiation, most commonly consumed substances, motivation and effects, peer group pressure and how they obtained the substances, and the perceptions held and main activities carried out in the educational institutions and health centers.

CD32 Expression is not Associated to HIV-DNA content in CD4 cell subsets of individuals with Different Levels of HIV Control.

A recent study has pointed out to CD32a as a potential biomarker of HIV-persistent CD4 cells. We have characterized the level and phenotype of CD32+ cells contained in different subsets of CD4 T-cells and its potential correlation with level of total HIV-DNA in thirty HIV patients (10 typical progressors naïve for cART, 10 cART-suppressed patients, and 10 elite controllers). Total HIV-DNA was quantified in different subsets of CD4 T-cells: Trm and pTfh cells. Level and immunephenotype of CD32+ cells were analyzed in these same subsets by flow cytometry. CD32 expression in Trm and pTfh subsets was similar in the different groups, and there was no significant correlation between the level of total HIV-DNA and the level of CD32 expression in these subsets. However, total HIV-DNA level was correlated with expression of CD127 (rho = -0.46, p = 0.043) and of CCR6 (rho = -0.418, p = 0.027) on CD32+ cells. Our results do not support CD32 as a biomarker of total HIV-DNA content. However, analyzing the expression of certain markers by CD32+ cells could improve the utility of this marker in the clinical setting, prompting the necessity of further studies to both validate our results and to explore the potential utility of certain markers expressed by CD32+ cells.

Razonamiento, diseño y metodología del estudio de identificación de los atributos físicos del síndrome de temor a caerse en ancianos (FISTAC) / Rationale, design and methodology of physical attributes identification of the fear of falling syndrome (FISTAC study)

Introducción. El objetivo del estudio es identificar los atributos físicos del síndrome de temor a caerse (STAC) en mayores con caídas previas. Metodología. Estudio observacional analítico sobre 183 sujetos mayores de 64 años que hayan sufrido al menos una caída en el último año, captados desde la consulta de geriatría del Complejo Hospitalario Universitario de Albacete. De ellos, 140 cumplían criterios de STAC, y los otros 43 no (grupo control). Como covariables se recogieron datos sociodemográficos, antropométricos, de comorbilidad y fármacos, situación funcional, función física, fragilidad, estado cognitivo y afectivo. Se determinó la masa muscular mediante bioimpedanciometría (BIA) y densitometría (DXA), la fuerza prensora mediante dinamómetro digital de JAMAR, la fuerza extensora (1RM) de miembros inferiores, la potencia muscular de miembros inferiores mediante el instrumento T-Force, la variabilidad de la marcha con el instrumento Gait-Rite, las alteraciones posturales mediante posturografía. Se analizará si el STAC se asocia con alteraciones físicas ajustado por las covariables de estudio. Resultados. Edad media 78,4 años, 147 mujeres. En 182 participantes se pudo realizar posturografía, en 146 se pudo determinar potencia muscular de piernas, en 117 se realizó DXA y en 165, BIA. El STAC se asoció a sexo femenino, estado de fragilidad, ánimo deprimido, riesgo social, fuerza y potencia muscular, función física, número de fármacos e hipotensión ortostática en la muestra global, pero al ajustar por sexo, solo la fragilidad, el ánimo deprimido y el consumo de fármacos se asociaron al STAC. Conclusiones. Se presenta el razonamiento, el diseño y la metodología del estudio FISTAC (AU)

Introduction. The aim of this study was to identify the physical determining factors of the Fear of Falling Syndrome (FoF) in older adults with a history of falls. Methods. An observational study was conducted on 183 subjects older than 64 years with a fall in the previous year, with data collected from the geriatrics outpatient clinic of the Complejo Hospitalario Universitario from Albacete, Spain. Sociodemographic and anthropometric data, as well as comorbidity, drugs usually taken, functional status, physical function, frailty, cognitive and affective status were collected. Muscle mass was measured using bioimpedancy meter (BIA), and densitometry (DXA), strength with digital hand-held JAMAR dynamometer and with a Leg-press machine, muscle potency with a T-Force instrument, gait variability with the Gait-Rite instrument, and postural stability with the Neurocom Balance Master posturograph were also determined. An analysis was performed to determine if the FoF is associated with physical impairments adjusted for the study covariates. Results. The study included 140 subjects with FoF, and 43 without it. The mean age was 78.4 years, and 147 were women. Posturography could be measured in 182 participants, DXA in 117, BIA in 165, and muscle potency in 146. FoF was associated with female sex, frailty, depressed mood, social risk, muscle strength and power, physical function, number of drugs used, and orthostatic hypotension in the overall sample. After adjusting for sex, only frailty, depressed mood, and number of drugs remained associated. Conclusions. Rationale, design, and methods of the FISTAC study are presented (AU)

[Rationale, design and methodology of physical attributes identification of the fear of falling syndrome (FISTAC study)]. / Razonamiento, diseño y metodología del estudio de identificación de los atributos físicos del síndrome de temor a caerse en ancianos (FISTAC).

INTRODUCTION: The aim of this study was to identify the physical determining factors of the Fear of Falling Syndrome (FoF) in older adults with a history of falls. METHODS: An observational study was conducted on 183 subjects older than 64 years with a fall in the previous year, with data collected from the geriatrics outpatient clinic of the Complejo Hospitalario Universitario from Albacete, Spain. Sociodemographic and anthropometric data, as well as comorbidity, drugs usually taken, functional status, physical function, frailty, cognitive and affective status were collected. Muscle mass was measured using bioimpedancy meter (BIA), and densitometry (DXA), strength with digital hand-held JAMAR dynamometer and with a Leg-press machine, muscle potency with a T-Force instrument, gait variability with the Gait-Rite instrument, and postural stability with the Neurocom Balance Master posturograph were also determined. An analysis was performed to determine if the FoF is associated with physical impairments adjusted for the study covariates. RESULTS: The study included 140 subjects with FoF, and 43 without it. The mean age was 78.4 years, and 147 were women. Posturography could be measured in 182 participants, DXA in 117, BIA in 165, and muscle potency in 146. FoF was associated with female sex, frailty, depressed mood, social risk, muscle strength and power, physical function, number of drugs used, and orthostatic hypotension in the overall sample. After adjusting for sex, only frailty, depressed mood, and number of drugs remained associated. CONCLUSIONS: Rationale, design, and methods of the FISTAC study are presented.

Multicentric study of the effect of pre-analytical variables in the quality of plasma samples stored in biobanks using different complementary proteomic methods.

Analytical proteomics has experienced exponential progress in the last decade and can be expected to lead research studies on diagnostic and therapeutic biomarkers in the near future. Because the development of this type of analysis requires the use of a large number of human samples with a minimum of quality requirements, our objective was to identify appropriate indicators for quality control of plasma samples stored in biobanks for research in proteomics. To accomplish this, plasma samples from 100 healthy donors were obtained and processed according to the pre-analytical variables of: a) time delay for the first centrifugation of the original blood sample (4 or 24h) and b) number of freeze/thaw cycles (1, 2 or 3) of the processed plasma samples. The analyses of samples were performed by different and complementary methods such as SPE MALDI-TOF, DIGE, shotgun (iTRAQ, nLC MALDI TOF/TOF) and targeted nLC MS/MS proteomic techniques (SRM). In general, because the distribution of proteins in all samples was found to be very similar, the results shown that delayed processing of blood samples and the number of freeze/thaw cycles has little or no effect on the integrity of proteins in the plasma samples. SIGNIFICANCE: The results of the present work indicate that blood proteins in plasma are broadly insensitive to such preanalytical variables as delayed processing or freeze/thaw cycles when analyzed at the peptide level. Although there are other studies related to protein stability of clinical samples with similar results, what is remarkable about our work is the large number of plasma samples examined and that our analyses assessed protein integrity by combining a wide set of complementary proteomic approaches performed at different proteomic platform participating laboratories that all yielded similar results. We believe our study is the most comprehensive performed to date to determine the changes in proteins induced by delayed sample processing and plasma freeze/thaw cycles.

Varón marroquí con espondilodiscitis de larga evolución, orquitis y endocarditis / Moroccan man with longstanding spondylodiscitis, orchitis and endocarditis

La afectación osteoarticular es la complicación más frecuente de la brucelosis. La espondilodiscitis es una complicación grave, ya que suele estar asociada con la formación de abscesos. Se presenta el caso de un varón de 43 años de edad, natural de Marruecos, con síntomas, signos y hallazgos imagenológicos de espondilodiscitis de larga evolución, orquitis y endocarditis. Mediante la realización de pruebas de laboratorio complementarias se llega al diagnóstico de infección por Brucella spp. El presente caso busca concientizar a la comunidad médica sobre la importancia de considerar la infección por Brucella spp. como diagnóstico diferencial en pacientes con sospecha de espondilodiscitis infecciosa, especialmente en aquellos pacientes con antecedentes epidemiológicos, permitiendo así realizar un acertado y oportuno diagnóstico. Este caso resulta interesante porque nunca antes se había descrito la presencia simultánea de estas tres manifestaciones de la brucelosis en un paciente inmuno competente. (Acta Med Colomb 2015; 40: 166-168).

Osteoarticular involvement is the most common complication of brucellosis. Spondylodiscitis is a serious complication as it is often associated with abscess formation. The case of a 43 years old man born in Morocco with symptoms, signs and imaging findings of longstanding spondylodiscitis, orchitis and endocarditis is presented. Through the implementation of complementary laboratory tests, diagnosis infection by Brucella spp. was made.This case aims to raise awareness in the medical community about the importance of considering Brucella spp. as a differential diagnosis in patients with suspected infectious spondylodiscitis, especially in those patients with epidemiological background, allowing make a successful and timely diagnosis. This case is interesting because never before had been described the simultaneous presence of these three manifestations of brucellosis in an immunocompetent patient. (Acta Med Colomb 2015; 40: 166-168).

The daily life of pregnant women: nursing promoting being healthy / Lo cotidiano de mujeres embarazadas: la enfermería promoviendo el ser saludable / O quotidiano de gestantes: a enfermagem promovendo o ser saudável

This is a qualitative, exploratory-descriptive study, grounded on the everyday life and interpretative sociology. The aim of the study was to understand the everyday life of pregnant women and their families, based on prenatal care. The study was conducted in a health center in southern Brazil, and it involved ten pregnant women. Data were collected by means of semi-structured interviews, using the intrafamilial genogram and ecomap, between March and May of 2011. Data analysis involved: preliminary analysis, ordering, key links, coding and categorization. The results show that the everyday life of pregnant women involves biological changes and emotional experiences that generate a need for changing their pace of life, whose implementation depends on social changes, requiring a support network that may contribute to promote health during pregnancy.

Se trata de un estudio cualitativo, exploratório-descriptivo, basado en la Sociología Comprensiva y el Cotidiano. Hemos tratado de entender el cotidiano de las mujeres en el ámbito familiar, desde el período prenatal. La investigación se realizó en un Centro de Salud en el sur de Brasil, con diez mujeres embarazadas. La recogida de datos fue a través de entrevistas semi-estructuradas utilizando el Genograma Intra-familiar y Eco-mapa, entre marzo y mayo de 2011. El análisis de los datos: análisis preliminar, clasificación, palabras clave, codificación y categorización. Los resultados muestran que la vida cotidiana de las mujeres embarazadas implica la modificación de algunas experiencias biológicas y emocionales que generan una necesidad de cambios en el ritmo de la vida, cuya implementación depende de cambios de orden social, que requieren una red de apoyo que pueden contribuir a la promoción de salud durante el embarazo.

Trata-se de uma pesquisa qualitativa, exploratório-descritiva, fundamentada na Sociologia Compreensiva e do Quotidiano. Buscou-se compreender o quotidiano das gestantes, no contexto familiar, a partir do pré-natal. A pesquisa foi realizada em Centro de Saúde do Sul do Brasil, com dez gestantes. A coleta de dados deu-se através de entrevistas semiestruturadas, utilizando o genograma intrafamiliar e ecomapa, entre março e maio de 2011. A análise de dados envolveu: análise preliminar, ordenação, ligações-chave, codificação e categorização. Os resultados mostram que o quotidiano das gestantes envolve modificações de ordem biológica e vivências de ordem emocional que geram uma necessidade de modificações no ritmo de vida, cuja concretização depende de modificações de ordem social, demandando uma rede de apoio que pode contribuir para a promoção da saúde na gestação.

Sexual behavior in the everyday life of adolescents and young adults from the hip hop culture / Comportamento sexual no cotidiano de adolescentes e jovens da cultura hip hop / Conductas sexuales en el cotidiano de adolescentes y jóvenes de la cultura hip hop

This is a quantitative, cross-sectional, descriptive study, conducted in two consecutive series in 2007 (S1) and 2008 (S2), comprised of 350 people between 15 and 29 years of age, with the aim to understand the profile of adolescents and young adults from the hip hop culture of Seville, Spain, and to detect risky sexual behavior in their everyday lives. Data were obtained by means of a self-administered questionnaire followed by a statistical analysis, corresponding to a sexually active population (91%), who initiated their sexual experiences before 16.5 years of age. Most state they are heterosexual and use contraception. In conclusion, the image of this group is tied to virile masculine stereotypes, constituting the imaginary of this body of people. Direct interventions are needed to promote health and disease prevention, before adolescence, involving health services, families and schools, which address sexual education and love, taking into account the social construct and everyday life.

Estudo quantitativo descritivo transversal, realizado em duas séries consecutivas, correspondendo a 2007 (S1) e 2008 (S2), abrangendo 350 pessoas, entre 15-29 anos, com objetivo de conhecer o perfil dos adolescentes e jovens da cultura hip hop em Sevilha, Espanha, detectando comportamentos sexuais de risco no cotidiano. Coletou-se dados através de questionário autoadministrado, com análise estatística. A população é sexualmente ativa (91%), iniciando suas experiências sexuais antes dos 16,5 anos. A maioria relata heterossexualidade e usa métodos contraceptivos. Conclui-se que a imagem deste coletivo liga-se a estereótipos masculinos viris, constituindo o imaginário e cotidiano do grupo. Faz-se necessário dirigir intervenções para promoção da saúde e prevenção de agravos, antes da adolescência, nos serviços de saúde, na família e escola, envolvendo educação sexual e amorosa, considerando o imaginário social e o cotidiano.

Estudio cuantitativo de corte transversal realizado en dos series consecutivas, correspondientes a 2007 (S1) y 2008 (S2), que comprende 350 personas, entre 15-29 años, con objetivo de conocer el perfil de los adolescentes y jóvenes en la cultura hip hop de Sevilla, España, detectar comportamientos sexuales de riesgo en la vida cotidiana. Los datos fueron recogidos a través de cuestionario autoaplicado con análisis estadístico. Población sexualmente activa (91%), iniciando sus experiencias sexuales antes de 16,5 años. La mayoría reportes heterosexualidad, usa anticonceptivos. Se concluye que la imagen de este colectivo se une a los estereotipos masculinos viriles, constituyendo el imaginario diario del grupo. Es necesario intervenciones directas para la promoción de la salud y prevención de enfermedades, antes de la adolescencia, de servicios de salud, la familia y la escuela, que implica la educación sexual y el amor, teniendo en cuenta el imaginario social y lo cotidiano.

Correlation between different methods to measure microbial translocation and its association with immune activation in long-term suppressed HIV-1-infected individuals.

INTRODUCTION: Microbial translocation (MT) has been proposed as one of the triggering mechanisms of persistent immune activation associated to HIV-1 infection. Our objectives were to determine the correlation between different measurements of MT in suppressed HIV-1-infected individuals and to evaluate its correlation with immune activation. METHODS: Eighteen suppressed HIV-1-infected patients with CD4+ T-cell count above 350 cells per cubic millimeter and undetectable plasma viral load, included in antiretroviral treatment intensification clinical trials, were evaluated. Samples obtained at baseline and at established time points during the trials were analyzed. Lipopolysaccharide (LPS), lipopolysaccharide binding protein (LBP), soluble CD14 (sCD14), and bacterial 16S ribosomal DNA (16S rDNA), and markers of immune activation were determined. RESULTS: We analyzed 126 plasma samples from the 18 patients. LPS significantly correlated with sCD14 (P < 0.001, r = 0.407) and LBP (P = 0.042, r = 0.260). Also, a significant correlation was found between sCD14 and LBP (P = 0.009, r = 0.325) but not between bacterial 16S rDNA and LPS, sCD14, or LBP (P = 0.346, P = 0.405, and P = 0.644). On the other hand, no significant correlation was found between LPS, sCD14, or LBP and CD4 (P = 0.418, P = 0.619, and P = 0.728) or CD8 T-cell activation (P = 0.352, P = 0.275, and P = 0.124). Bacterial 16S rDNA correlated with activated CD4 T cells (P = 0.005, r = 0.104) but not with activated CD8 T cells (P = 0.171). CONCLUSIONS: There is a good correlation in the quantification of LPS, sCD14, and LBP levels, but not with bacterial 16S rDNA, as measurements of MT. We are unable to ensure that MT directly triggers T-cell immune activation at least among these patients with relatively good immune recovery and under treatment intensification.

Nuevos diagnósticos de infección VIH en niños / New diagnosis of HIV infection in children

Introducción En la cohorte de niños infectados de la Comunidad de Madrid ha aumentado el número de niños de procedencia extranjera en los últimos años. Los objetivos fueron evaluar las características epidemiológicas y clínicas en los nuevos diagnósticos y describir los diferentes subtipos del VIH-1.Pacientes y métodos Se analizaron los nuevos diagnósticos desde el año 1997, dividiéndolos en 3 periodos: P1 (1997-2000), P2 (2001-2004), P3 (2005-2009). Se analizó la procedencia según regiones geográficas y país de procedencia, las diferencias clínicas e inmunovirológicas así como respuesta al tratamiento. Se evaluó el subtipo genético del VIH-1 mediante análisis filogenético de los genes de proteasa y de la retrotranscriptasa. Resultados Se identificaron 141 nuevos diagnósticos de infección VIH, siendo el porcentaje de procedencia extranjera en P1 (22,5%), P2 (50%) y P3 (68%). La procedencia ha cambiado de Latinoamérica en P1 a África subsahariana en P3. No hubo diferencias de la media de edad al diagnóstico entre autóctonos y extranjeros, el estadio clínico CDC A/B/C, carga viral, porcentaje de CD4 al diagnóstico y actuales. Había una tendencia de mejor respuesta virológica en extranjeros tras el primer ciclo de TARGA (terapia antirretroviral de gran actividad) independiente del tratamiento recibido. Se obtuvieron 66 subtipos, el 24% eran subtipos no-B (56% formas recombinantes). Todos los subtipos de los autóctonos (43) y latinoamericanos (5) eran subtipos B, sin embargo, todos los niños procedentes de África Subsahariana (14) eran subtipos no-B. Conclusión No se encontraron diferencias entre niños infectados por VIH extranjeros o autóctonos, salvo los diferentes subtipos de VIH-1 (AU)

Introduction The number of children of immigrant origin in the last few years has increased the cohort of HIV-infected children in the Community of Madrid. The objectives of the study were to evaluate the epidemiological and clinical characteristics of the new diagnosed children and describe the different subtypes of HIV-1.Patients and methods The new diagnosed children were analysed from the year 1997, divided into 3 periods: P1 (1997-2000), P2 (2001-2004), P3 (2005-2009). The regions and countries of origin, the clinical, immune and viral characteristics, as well as the response to treatment were analysed. The subtypes of HIV-1 were evaluated by phylogenetic analysis of protease genes and reverse transcriptase. Results We identified 141 new diagnoses of HIV infection, the percentage of immigrant origin in P1 was (22.5%), P2 (50%) and P3 (68%). The origin had changed from Latin America in P1 to sub-Saharan Africa in P3. There were no differences between Spanish and immigrant children in the age at diagnosis, the CDC clinical stage A/B/C, viral load, percentage of CD4 at diagnosis and actual. Better viral response was more likely in immigrants after the first regimen of HAART (Highly active antiretroviral treatment) independently of the treatment received. A total of 66 subtypes were obtained, 24% were subtypes non-B (56% recombinants forms). All subtypes of Spanish children (43) and Latin American (5) were subtypes B, and all the children from sub-Saharan Africa (14) were subtypes non-B. Conclusion There were no differences between immigrants and Spanish children infected by HIV, except the different subtypes of HIV-1 (AU)